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The map label for this gene is metG [H]

Identifier: 167038739

GI number: 167038739

Start: 67845

End: 69743

Strand: Direct

Name: metG [H]

Synonym: Teth514_0066

Alternate gene names: 167038739

Gene position: 67845-69743 (Clockwise)

Preceding gene: 167038738

Following gene: 167038740

Centisome position: 2.76

GC content: 35.76

Gene sequence:

>1899_bases
ATGAAAAAGACTTTTTACATTACGACACCTATCTATTATCCCAGTGATAAATTACATATTGGTCATTCATATACTACTGT
TGCTGCAGATGCGATGGCAAGATTTAAAAGGCTTACCGGATACGATGTCAAGTTTTTGACTGGTACTGATGAACATGGGC
AAAAAATTCAAAGAATAGCCAGAGAAAAAGGGATGTCTCCTAAAGAATATGTAGATGGGATAGTTGAATGGATAAAAGAT
TTATGGAAGATAATGGATATAAGTTATGACCAATTTATTCGCACAACTGATAAATATCATGAGGAAATAGTACAAAAAAT
ATTCACGAAATTATATGAAAAAGGAGATATATACAAGAGCAAATATGAAGGATGGTACTGTACTCCTTGTGAATCTTTCT
GGACTGAGAGCCAGCTTGTAGATGGGAAATGCCCAGACTGTGGTAGACAAGTAGAAAGGGTAAAAGAAGAAGGGTATTTC
TTTAGATTGTCTAAATATGGGGATAGGCTGTTACAATATTACGATGAACATCCTGATTTTATACAGCCAGAGTCAAGACG
CAATGAGATGATAAACTTTATAAAATCAGGTTTGGAGGATTTATTTGTTTCAAGGAGTTCGTTTGACTGGGGTATAAAGG
TACCTTTTGACCCAAAACATGTGATTTATGTTTGGATAGATGCTTTGTCAAATTACATAACTGCCTTAGGATATTCTACA
GACCATGACGACGATTTTAAAAAATACTGGCCAGCAGATGTTCATTTGGTTGGAAAAGAGATAATGAGGTTCCACACAAT
AATATGGCCAGCAATGCTTATGGCTCTTGACTTGCCTCTTCCTAAAAAAGTGTTTGGACACGGTTGGCTTATACTGGAAG
GAGGCAAGATGTCAAAATCTAAAGGTAATGTAGTAGACCCTAAAGAGTTAGTTAGTAAATATGGTGTAGATGCTGTAAGA
TATTTTCTTTTGAGAGAAGTTCCTTTTGGGGCGGATGGAGTGTTTTCTAACGAAGCTTTAATAAATAGAATAAATTCAGA
CCTTGCTAATGACCTTGGAAACTTGCTGAGTAGGACTGTTACAATGATTGAAAAATACTTTGGAGGCATTCTGCCAAAAC
CTTCACAAAAAGAAGAGGTAGATGATGACCTTATAACTACTGCACAGAATCTGCCGAAAACCGTTGAGGAGTATATGGAT
AAACTTCAGTTTTCCAACGCTTTAATTGAAATATGGAAATTGGTAAGACGAGCGAATAAATATATCGATGAAACGATGCC
TTGGGTTTTAGCTAAAGATGAAAGCAAAAAGGAAAGATTAGGTACAGTTTTATATAATTTGACGGAAGCTTTGAGGTTTA
TTGCAGTACTTATATCTCCTTTTATGCCCAATACTCCAAAAAAAATCTTCGAGCAGTTAGGAGTAGAAGAGAATTTAACT
ACTTGGGAAAGCTTAAAATTTGGCCTGTTAAAGGAAGGCACAAAGGTGAAAAGGGGAGAAATTATATTCCCAAGGATAGA
TGTAAAGAAGGAACTGGTTGCTATAGAAGAAAAAGCAAAGGAAGAGAAAAAGATAGATTACATCACTATTGAAGAGTTTG
CAAAAATTCAGTTGAGAGTAGCTGAAATTTTAGAAGCAGAAAGAGTGGAAGGTTCTGACAAACTTATAAAGATGAAGCTT
AAAGTGGGAGAAGAAATAAGACAAATTGTGGGAGGAATTGGTCGCCATTATGCTCCTAAAGAGCTTGTAGGAAAGAAAAT
AATTATTGTATATAATCTGGAGCCAAAGAAACTAAAGGGTATTGAATCTCAAGGAATGCTTTTGGCAGCATCTACCGAAG
GAAAAATGTCTCTTCTTACGGTAGACAGGGATATAGAAAGTGGGGCAAAGATAAGCTAA

Upstream 100 bases:

>100_bases
CATGCGTCGTGAGGTGTATGCGAAGAAGGGTGGTACCGCGACAATCTCGCCCCTTATGTGGAGGCGAGTTTTTTATTTAC
AAAAAAAGAGGAGGATGGAT

Downstream 100 bases:

>100_bases
GGAGTGATAAAATTGTTGGTGGATTCTCACGCTCATCTTGAAGATGAGAAATACGATAAAGATAGAGAAGAAGTTATTGA
AGAATGCAAAAAAGATCTCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVKFLTGTDEHGQKIQRIAREKGMSPKEYVDGIVEWIKD
LWKIMDISYDQFIRTTDKYHEEIVQKIFTKLYEKGDIYKSKYEGWYCTPCESFWTESQLVDGKCPDCGRQVERVKEEGYF
FRLSKYGDRLLQYYDEHPDFIQPESRRNEMINFIKSGLEDLFVSRSSFDWGIKVPFDPKHVIYVWIDALSNYITALGYST
DHDDDFKKYWPADVHLVGKEIMRFHTIIWPAMLMALDLPLPKKVFGHGWLILEGGKMSKSKGNVVDPKELVSKYGVDAVR
YFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTVTMIEKYFGGILPKPSQKEEVDDDLITTAQNLPKTVEEYMD
KLQFSNALIEIWKLVRRANKYIDETMPWVLAKDESKKERLGTVLYNLTEALRFIAVLISPFMPNTPKKIFEQLGVEENLT
TWESLKFGLLKEGTKVKRGEIIFPRIDVKKELVAIEEKAKEEKKIDYITIEEFAKIQLRVAEILEAERVEGSDKLIKMKL
KVGEEIRQIVGGIGRHYAPKELVGKKIIIVYNLEPKKLKGIESQGMLLAASTEGKMSLLTVDRDIESGAKIS

Sequences:

>Translated_632_residues
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVKFLTGTDEHGQKIQRIAREKGMSPKEYVDGIVEWIKD
LWKIMDISYDQFIRTTDKYHEEIVQKIFTKLYEKGDIYKSKYEGWYCTPCESFWTESQLVDGKCPDCGRQVERVKEEGYF
FRLSKYGDRLLQYYDEHPDFIQPESRRNEMINFIKSGLEDLFVSRSSFDWGIKVPFDPKHVIYVWIDALSNYITALGYST
DHDDDFKKYWPADVHLVGKEIMRFHTIIWPAMLMALDLPLPKKVFGHGWLILEGGKMSKSKGNVVDPKELVSKYGVDAVR
YFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTVTMIEKYFGGILPKPSQKEEVDDDLITTAQNLPKTVEEYMD
KLQFSNALIEIWKLVRRANKYIDETMPWVLAKDESKKERLGTVLYNLTEALRFIAVLISPFMPNTPKKIFEQLGVEENLT
TWESLKFGLLKEGTKVKRGEIIFPRIDVKKELVAIEEKAKEEKKIDYITIEEFAKIQLRVAEILEAERVEGSDKLIKMKL
KVGEEIRQIVGGIGRHYAPKELVGKKIIIVYNLEPKKLKGIESQGMLLAASTEGKMSLLTVDRDIESGAKIS
>Mature_632_residues
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVKFLTGTDEHGQKIQRIAREKGMSPKEYVDGIVEWIKD
LWKIMDISYDQFIRTTDKYHEEIVQKIFTKLYEKGDIYKSKYEGWYCTPCESFWTESQLVDGKCPDCGRQVERVKEEGYF
FRLSKYGDRLLQYYDEHPDFIQPESRRNEMINFIKSGLEDLFVSRSSFDWGIKVPFDPKHVIYVWIDALSNYITALGYST
DHDDDFKKYWPADVHLVGKEIMRFHTIIWPAMLMALDLPLPKKVFGHGWLILEGGKMSKSKGNVVDPKELVSKYGVDAVR
YFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTVTMIEKYFGGILPKPSQKEEVDDDLITTAQNLPKTVEEYMD
KLQFSNALIEIWKLVRRANKYIDETMPWVLAKDESKKERLGTVLYNLTEALRFIAVLISPFMPNTPKKIFEQLGVEENLT
TWESLKFGLLKEGTKVKRGEIIFPRIDVKKELVAIEEKAKEEKKIDYITIEEFAKIQLRVAEILEAERVEGSDKLIKMKL
KVGEEIRQIVGGIGRHYAPKELVGKKIIIVYNLEPKKLKGIESQGMLLAASTEGKMSLLTVDRDIESGAKIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=542, Percent_Identity=35.239852398524, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI14043022, Length=524, Percent_Identity=27.0992366412214, Blast_Score=180, Evalue=4e-45,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=28.82096069869, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1786861, Length=192, Percent_Identity=28.6458333333333, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=32.1100917431193, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=34.5029239766082, Blast_Score=291, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI71992781, Length=403, Percent_Identity=33.2506203473945, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17540794, Length=555, Percent_Identity=27.027027027027, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71997510, Length=168, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71997517, Length=168, Percent_Identity=32.1428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=32.1495327102804, Blast_Score=270, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=25.3597122302158, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6323414, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358483, Length=504, Percent_Identity=33.531746031746, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24655311, Length=557, Percent_Identity=26.3913824057451, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 72969; Mature: 72969

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVKFLTGTDEHGQKIQRIA
CCCEEEEECCEECCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
REKGMSPKEYVDGIVEWIKDLWKIMDISYDQFIRTTDKYHEEIVQKIFTKLYEKGDIYKS
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KYEGWYCTPCESFWTESQLVDGKCPDCGRQVERVKEEGYFFRLSKYGDRLLQYYDEHPDF
HCCCEEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCC
IQPESRRNEMINFIKSGLEDLFVSRSSFDWGIKVPFDPKHVIYVWIDALSNYITALGYST
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHCCCC
DHDDDFKKYWPADVHLVGKEIMRFHTIIWPAMLMALDLPLPKKVFGHGWLILEGGKMSKS
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCC
KGNVVDPKELVSKYGVDAVRYFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTV
CCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TMIEKYFGGILPKPSQKEEVDDDLITTAQNLPKTVEEYMDKLQFSNALIEIWKLVRRANK
HHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDETMPWVLAKDESKKERLGTVLYNLTEALRFIAVLISPFMPNTPKKIFEQLGVEENLT
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
TWESLKFGLLKEGTKVKRGEIIFPRIDVKKELVAIEEKAKEEKKIDYITIEEFAKIQLRV
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
AEILEAERVEGSDKLIKMKLKVGEEIRQIVGGIGRHYAPKELVGKKIIIVYNLEPKKLKG
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCHHHHCC
IESQGMLLAASTEGKMSLLTVDRDIESGAKIS
CCCCCEEEEECCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYDVKFLTGTDEHGQKIQRIA
CCCEEEEECCEECCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
REKGMSPKEYVDGIVEWIKDLWKIMDISYDQFIRTTDKYHEEIVQKIFTKLYEKGDIYKS
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KYEGWYCTPCESFWTESQLVDGKCPDCGRQVERVKEEGYFFRLSKYGDRLLQYYDEHPDF
HCCCEEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCC
IQPESRRNEMINFIKSGLEDLFVSRSSFDWGIKVPFDPKHVIYVWIDALSNYITALGYST
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHCCCC
DHDDDFKKYWPADVHLVGKEIMRFHTIIWPAMLMALDLPLPKKVFGHGWLILEGGKMSKS
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCC
KGNVVDPKELVSKYGVDAVRYFLLREVPFGADGVFSNEALINRINSDLANDLGNLLSRTV
CCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TMIEKYFGGILPKPSQKEEVDDDLITTAQNLPKTVEEYMDKLQFSNALIEIWKLVRRANK
HHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDETMPWVLAKDESKKERLGTVLYNLTEALRFIAVLISPFMPNTPKKIFEQLGVEENLT
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
TWESLKFGLLKEGTKVKRGEIIFPRIDVKKELVAIEEKAKEEKKIDYITIEEFAKIQLRV
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
AEILEAERVEGSDKLIKMKLKVGEEIRQIVGGIGRHYAPKELVGKKIIIVYNLEPKKLKG
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCHHHHCC
IESQGMLLAASTEGKMSLLTVDRDIESGAKIS
CCCCCEEEEECCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]