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The map label for this gene is cca [H]

Identifier: 167038738

GI number: 167038738

Start: 66106

End: 67509

Strand: Direct

Name: cca [H]

Synonym: Teth514_0065

Alternate gene names: 167038738

Gene position: 66106-67509 (Clockwise)

Preceding gene: 167038737

Following gene: 167038739

Centisome position: 2.69

GC content: 31.13

Gene sequence:

>1404_bases
GTGGCAACAATACCAAAATTAATAAGAAAATTAGGAAAAGAAGCATATAATTTGTATATTGTTGGGGGATACATAAGGGA
CAGGATTTTAAATAGAGAAGCTAAAGATTATGATTTTGTAGTTCAGGAAGATGCAGAGAAAATCGCCAAATTGGTGGCAG
AAAAATTAGGGGGTACTTTTGTGCCTTTTATGGCGGAAAAAGGCACATATAGGGTTGTGGTAGGAGATGAAATATTGGAT
TTTACTAATTTAAGGGGTGAAGACATATACATAGATTTAGCTCATAGAGACTTTACAATGAATGCTATGGCTATAAAACT
GACGGATTATTTTGAATTTGAATATATTATTGATCCTTTTGGTGGCATGACGGACATAAAAAACAGGAAAATAAAGCATG
TTGGAATGATGACTTTTAAAGAAGACCCTTTGAGGGCTTTGAGAGCTGTAAGATTTGCTGTAACTTACAAATTTGATATT
GATGAGAGTACAAAGGCTAAAATAAGAGAAGAAGCAGAGCTTATAAAACAAGTAGCGCCAGAGAGAATAATGAATGAAAT
TTTTATAATATTAAAAGAAAAGAATTCTCACAAATATTTAAGAATGATGGAAGATTTAAAACTTATGGAAAATATATTTG
AAGAAGTTGCTAAAATGAAAGAAGTAGGAAAGTGCTATTATCACCTTACGAATGCTTGGATACATTCACTAAAAACTGTA
GAAGAATACGAAAATATCATAAATTCTATGAGATTTCCCAAAGACGTATATGATATAGTTTCGGAGTATTTAGATAGAAA
GCTTTCTTCTGACAACAAAGTAAAGGACATAATAAAACTTGGAGCTCTTTTTCACGATATAGGCAAACCAGAATCCATAT
ACATTGATACTGAAAATAGAGTACATTTTTACAACCACGAGACAAAAGGAGCAGAAATCGTAAAAAAGATAGCATCAAGG
ATGAAAATGCCCAAAAAAGAGTCTTCTTTGCTAAAGAAGCTTGTTCTTTATCATATGAAGCCTTTGATGTACTATGTAAA
TGGCGGACCAACGAATAAGTCTCTTTTTAGATTTTTTTCCGAACTTAAGGATGATTCAATAGCGATTTTACTTTTATCTC
AGGCTGATTTTACTGCTACAAGGCTTAATTTAGGCAAAGAGGAAGAAATACCTAGGTATACAGATTTTATTACCAGACTT
TTAAAAAGGTATGTAGAATTTAAAGAAACAGAAAAACCATTACTTTCACCGCTGGATATAATAATAAATTTTGATGTAAA
GGATGGGAAAAAATTAGGACAGATTTTATATGAAATAAGGAAAAATAGATTTTACGGAGAAATTCAAGATAAAAACGATG
CGATTGAGTTTGTAAAAGAAAGAATGAAAATAGAGAAAGTATAA

Upstream 100 bases:

>100_bases
ATAGCAGATTATTTTTTTAATACTTGTATACTTTTTCACTTTTGTTATACTATATAGTGTAATGTTGTTTTGCCTTTTAA
AAGGAAAGGAATGATTTTTT

Downstream 100 bases:

>100_bases
AAGAAAAAGTAGTTGACAATACTTTTTATAAAGGGTATATTAAAGAATAAATAGATATTGTAAAAGGCAATGAAGGAAAG
AGTAAAAAGCTTGAACTTTT

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MATIPKLIRKLGKEAYNLYIVGGYIRDRILNREAKDYDFVVQEDAEKIAKLVAEKLGGTFVPFMAEKGTYRVVVGDEILD
FTNLRGEDIYIDLAHRDFTMNAMAIKLTDYFEFEYIIDPFGGMTDIKNRKIKHVGMMTFKEDPLRALRAVRFAVTYKFDI
DESTKAKIREEAELIKQVAPERIMNEIFIILKEKNSHKYLRMMEDLKLMENIFEEVAKMKEVGKCYYHLTNAWIHSLKTV
EEYENIINSMRFPKDVYDIVSEYLDRKLSSDNKVKDIIKLGALFHDIGKPESIYIDTENRVHFYNHETKGAEIVKKIASR
MKMPKKESSLLKKLVLYHMKPLMYYVNGGPTNKSLFRFFSELKDDSIAILLLSQADFTATRLNLGKEEEIPRYTDFITRL
LKRYVEFKETEKPLLSPLDIIINFDVKDGKKLGQILYEIRKNRFYGEIQDKNDAIEFVKERMKIEKV

Sequences:

>Translated_467_residues
MATIPKLIRKLGKEAYNLYIVGGYIRDRILNREAKDYDFVVQEDAEKIAKLVAEKLGGTFVPFMAEKGTYRVVVGDEILD
FTNLRGEDIYIDLAHRDFTMNAMAIKLTDYFEFEYIIDPFGGMTDIKNRKIKHVGMMTFKEDPLRALRAVRFAVTYKFDI
DESTKAKIREEAELIKQVAPERIMNEIFIILKEKNSHKYLRMMEDLKLMENIFEEVAKMKEVGKCYYHLTNAWIHSLKTV
EEYENIINSMRFPKDVYDIVSEYLDRKLSSDNKVKDIIKLGALFHDIGKPESIYIDTENRVHFYNHETKGAEIVKKIASR
MKMPKKESSLLKKLVLYHMKPLMYYVNGGPTNKSLFRFFSELKDDSIAILLLSQADFTATRLNLGKEEEIPRYTDFITRL
LKRYVEFKETEKPLLSPLDIIINFDVKDGKKLGQILYEIRKNRFYGEIQDKNDAIEFVKERMKIEKV
>Mature_466_residues
ATIPKLIRKLGKEAYNLYIVGGYIRDRILNREAKDYDFVVQEDAEKIAKLVAEKLGGTFVPFMAEKGTYRVVVGDEILDF
TNLRGEDIYIDLAHRDFTMNAMAIKLTDYFEFEYIIDPFGGMTDIKNRKIKHVGMMTFKEDPLRALRAVRFAVTYKFDID
ESTKAKIREEAELIKQVAPERIMNEIFIILKEKNSHKYLRMMEDLKLMENIFEEVAKMKEVGKCYYHLTNAWIHSLKTVE
EYENIINSMRFPKDVYDIVSEYLDRKLSSDNKVKDIIKLGALFHDIGKPESIYIDTENRVHFYNHETKGAEIVKKIASRM
KMPKKESSLLKKLVLYHMKPLMYYVNGGPTNKSLFRFFSELKDDSIAILLLSQADFTATRLNLGKEEEIPRYTDFITRLL
KRYVEFKETEKPLLSPLDIIINFDVKDGKKLGQILYEIRKNRFYGEIQDKNDAIEFVKERMKIEKV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=341, Percent_Identity=25.5131964809384, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081691, Length=236, Percent_Identity=27.5423728813559, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54844; Mature: 54712

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIPKLIRKLGKEAYNLYIVGGYIRDRILNREAKDYDFVVQEDAEKIAKLVAEKLGGTF
CCCHHHHHHHHCHHHEEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
VPFMAEKGTYRVVVGDEILDFTNLRGEDIYIDLAHRDFTMNAMAIKLTDYFEFEYIIDPF
EEHEECCCCEEEEECCHHHHHHCCCCCEEEEEEECCCEEECEEEEEEEHHEEEEEEECCC
GGMTDIKNRKIKHVGMMTFKEDPLRALRAVRFAVTYKFDIDESTKAKIREEAELIKQVAP
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
ERIMNEIFIILKEKNSHKYLRMMEDLKLMENIFEEVAKMKEVGKCYYHLTNAWIHSLKTV
HHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEYENIINSMRFPKDVYDIVSEYLDRKLSSDNKVKDIIKLGALFHDIGKPESIYIDTENR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
VHFYNHETKGAEIVKKIASRMKMPKKESSLLKKLVLYHMKPLMYYVNGGPTNKSLFRFFS
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHH
ELKDDSIAILLLSQADFTATRLNLGKEEEIPRYTDFITRLLKRYVEFKETEKPLLSPLDI
HCCCCCEEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
IINFDVKDGKKLGQILYEIRKNRFYGEIQDKNDAIEFVKERMKIEKV
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIPKLIRKLGKEAYNLYIVGGYIRDRILNREAKDYDFVVQEDAEKIAKLVAEKLGGTF
CCHHHHHHHHCHHHEEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
VPFMAEKGTYRVVVGDEILDFTNLRGEDIYIDLAHRDFTMNAMAIKLTDYFEFEYIIDPF
EEHEECCCCEEEEECCHHHHHHCCCCCEEEEEEECCCEEECEEEEEEEHHEEEEEEECCC
GGMTDIKNRKIKHVGMMTFKEDPLRALRAVRFAVTYKFDIDESTKAKIREEAELIKQVAP
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
ERIMNEIFIILKEKNSHKYLRMMEDLKLMENIFEEVAKMKEVGKCYYHLTNAWIHSLKTV
HHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEYENIINSMRFPKDVYDIVSEYLDRKLSSDNKVKDIIKLGALFHDIGKPESIYIDTENR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
VHFYNHETKGAEIVKKIASRMKMPKKESSLLKKLVLYHMKPLMYYVNGGPTNKSLFRFFS
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHH
ELKDDSIAILLLSQADFTATRLNLGKEEEIPRYTDFITRLLKRYVEFKETEKPLLSPLDI
HCCCCCEEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
IINFDVKDGKKLGQILYEIRKNRFYGEIQDKNDAIEFVKERMKIEKV
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA