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The map label for this gene is leuD [H]

Identifier: 167038691

GI number: 167038691

Start: 19087

End: 19569

Strand: Direct

Name: leuD [H]

Synonym: Teth514_0016

Alternate gene names: 167038691

Gene position: 19087-19569 (Clockwise)

Preceding gene: 167038690

Following gene: 167038692

Centisome position: 0.78

GC content: 35.2

Gene sequence:

>483_bases
ATGCAAGGGAAAGCCATAAAATATGGGGACAATATAGATACAGACGTTATAATACCAGCGAGGTTTTTAAACACTTCAGA
TCCACAGGAATTGGCACAGCATTGTATGGAAGACTTAGACAAAGACTTTAAGAATAAAGTTAAAGAAGGAGACATATTGG
TGGTAGGTGAAAATTTTGGTTGTGGCTCTTCCAGAGAGCACGCGCCAATCGCTATTAAAGCATCTGGTATATCCTGTATA
ATTGCTAAGTCGTTTGCAAGAATTTTTTTCAGAAATGCGATAAATATAGGGCTTCCGATATTAGAATGCAAAGAGGCAGT
AGATGGGATTGAAGAAGGTGATATTGTTTTGGTTGATACAGATAATGGAATTATAAAAAATTTAACTAAAGGGACAGAAT
TTAAAGCTCAACCCTTTCCTGACTTTATAAAAGAAATAATGAAATATGGTGGACTTATAAATTATGTCAGAGAGAAGGTG
TGA

Upstream 100 bases:

>100_bases
GAATGGGACATCCTGAAAGCGAAGTGTATCTTTCAAGTCCAGCTGTTGCGGCTGCTTCGGCTATTGCAGGATATATCGTA
AGTCCAGAGGAGGTGGAATG

Downstream 100 bases:

>100_bases
CATTGTTTAAAATTGCAGTGCTGCCTGGAGATGGAATTGGACCTGAGGTCATAGAAGAAGGGTTAAAAGTTTTGTCTGCT
ATAGAAGACAAGTACAAACT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MQGKAIKYGDNIDTDVIIPARFLNTSDPQELAQHCMEDLDKDFKNKVKEGDILVVGENFGCGSSREHAPIAIKASGISCI
IAKSFARIFFRNAINIGLPILECKEAVDGIEEGDIVLVDTDNGIIKNLTKGTEFKAQPFPDFIKEIMKYGGLINYVREKV

Sequences:

>Translated_160_residues
MQGKAIKYGDNIDTDVIIPARFLNTSDPQELAQHCMEDLDKDFKNKVKEGDILVVGENFGCGSSREHAPIAIKASGISCI
IAKSFARIFFRNAINIGLPILECKEAVDGIEEGDIVLVDTDNGIIKNLTKGTEFKAQPFPDFIKEIMKYGGLINYVREKV
>Mature_160_residues
MQGKAIKYGDNIDTDVIIPARFLNTSDPQELAQHCMEDLDKDFKNKVKEGDILVVGENFGCGSSREHAPIAIKASGISCI
IAKSFARIFFRNAINIGLPILECKEAVDGIEEGDIVLVDTDNGIIKNLTKGTEFKAQPFPDFIKEIMKYGGLINYVREKV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=128, Percent_Identity=36.71875, Blast_Score=70, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=106, Percent_Identity=46.2264150943396, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=44.6601941747573, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17680; Mature: 17680

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKAIKYGDNIDTDVIIPARFLNTSDPQELAQHCMEDLDKDFKNKVKEGDILVVGENFG
CCCCEEEECCCCCCCEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
CGSSREHAPIAIKASGISCIIAKSFARIFFRNAINIGLPILECKEAVDGIEEGDIVLVDT
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEC
DNGIIKNLTKGTEFKAQPFPDFIKEIMKYGGLINYVREKV
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MQGKAIKYGDNIDTDVIIPARFLNTSDPQELAQHCMEDLDKDFKNKVKEGDILVVGENFG
CCCCEEEECCCCCCCEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
CGSSREHAPIAIKASGISCIIAKSFARIFFRNAINIGLPILECKEAVDGIEEGDIVLVDT
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEC
DNGIIKNLTKGTEFKAQPFPDFIKEIMKYGGLINYVREKV
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]