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The map label for this gene is leuB [H]

Identifier: 167038692

GI number: 167038692

Start: 19572

End: 20639

Strand: Direct

Name: leuB [H]

Synonym: Teth514_0017

Alternate gene names: 167038692

Gene position: 19572-20639 (Clockwise)

Preceding gene: 167038691

Following gene: 167038693

Centisome position: 0.8

GC content: 37.08

Gene sequence:

>1068_bases
TTGTTTAAAATTGCAGTGCTGCCTGGAGATGGAATTGGACCTGAGGTCATAGAAGAAGGGTTAAAAGTTTTGTCTGCTAT
AGAAGACAAGTACAAACTTAAATTTGATGTAAAAAAGTATCCTTTTGGGGGAGAAGCTATAGACAAATATGGCGTTCCCT
ATCCGGAAGAGACAAAAAATGCATGTCTTTCTAGTGATGCTGTTCTTTTGGGAGCAGTAGGGGGGCCTAAATGGGATGAC
CTTGAGGGGGATAAAAGGCCAGAAGCGGGTCTTCTGGCTTTAAGAAAAAGCCTAGGGGTTTATGCAAATCTAAGACCTGC
TGTTCTTTATCCTCCTTTAAAAGAAGCTTCTCCTTTAAAAAACGAGTTATTACAAGAGGGTTTAGATATTCTTGTGGTAA
GAGAACTTACTGGTGGTATTTATTTTGGACCAAGAGGAATAGTTGCTATAGATTATGGCTTTAAGGCTTATGATACAGAA
GTGTACCAGACTTCAGAGATAGAAAGGATAGCGGTTATTGCCTTTGAAGCGGCTTTAAAGAGGAAAAAGAAAGTCACTTC
TGTAGACAAGGCAAACATTTTAGATTCTTCAAGATTGTGGAGAAAAACGGTAGAAGAAATAAGTAAGAGATATTCTGATG
TTGAGTTAAACCATATGTATGTAGATAACTGTGCTATGCAACTAATTAAAAATCCTTCTCAATTTGATGTAATTCTTACT
TCCAATATGTTTGGCGATATTTTAAGTGACGAGGCTTCCCAACTTACAGGTTCTATAGGTATGCTTCCTTCTGCTAGCTT
AAGAGAAGATAAAGTGGGACTTTATGAACCTATACACGGTTCTGCTCCAGATATTGCTGGAACAAAGAAGGCAAATCCTT
TAGCTACAATACTGTCAGTTGCTATGATGTTGGAATATTCTTTTAATCTACTTGATGCTGCTAATGACATAAAGGATGCA
GTAAATAAAGTTTTAGAGGCAGGTTATAGGACTTTTGATTTAGGAAAAGGAATAATTTTAAATACCCAACAAATGGGAGA
TAAAGTGGTGGAGTATATAAAGGGGTGA

Upstream 100 bases:

>100_bases
CTAAAGGGACAGAATTTAAAGCTCAACCCTTTCCTGACTTTATAAAAGAAATAATGAAATATGGTGGACTTATAAATTAT
GTCAGAGAGAAGGTGTGACA

Downstream 100 bases:

>100_bases
GGATATTGATAAGTGATAGCATAAAAAAGGGAGTTGAAAAGGCGCCACATAGGTCACTTTTATATGCTTTAGGCTTGACT
TATGAAGAAATAAAAAGGCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MFKIAVLPGDGIGPEVIEEGLKVLSAIEDKYKLKFDVKKYPFGGEAIDKYGVPYPEETKNACLSSDAVLLGAVGGPKWDD
LEGDKRPEAGLLALRKSLGVYANLRPAVLYPPLKEASPLKNELLQEGLDILVVRELTGGIYFGPRGIVAIDYGFKAYDTE
VYQTSEIERIAVIAFEAALKRKKKVTSVDKANILDSSRLWRKTVEEISKRYSDVELNHMYVDNCAMQLIKNPSQFDVILT
SNMFGDILSDEASQLTGSIGMLPSASLREDKVGLYEPIHGSAPDIAGTKKANPLATILSVAMMLEYSFNLLDAANDIKDA
VNKVLEAGYRTFDLGKGIILNTQQMGDKVVEYIKG

Sequences:

>Translated_355_residues
MFKIAVLPGDGIGPEVIEEGLKVLSAIEDKYKLKFDVKKYPFGGEAIDKYGVPYPEETKNACLSSDAVLLGAVGGPKWDD
LEGDKRPEAGLLALRKSLGVYANLRPAVLYPPLKEASPLKNELLQEGLDILVVRELTGGIYFGPRGIVAIDYGFKAYDTE
VYQTSEIERIAVIAFEAALKRKKKVTSVDKANILDSSRLWRKTVEEISKRYSDVELNHMYVDNCAMQLIKNPSQFDVILT
SNMFGDILSDEASQLTGSIGMLPSASLREDKVGLYEPIHGSAPDIAGTKKANPLATILSVAMMLEYSFNLLDAANDIKDA
VNKVLEAGYRTFDLGKGIILNTQQMGDKVVEYIKG
>Mature_355_residues
MFKIAVLPGDGIGPEVIEEGLKVLSAIEDKYKLKFDVKKYPFGGEAIDKYGVPYPEETKNACLSSDAVLLGAVGGPKWDD
LEGDKRPEAGLLALRKSLGVYANLRPAVLYPPLKEASPLKNELLQEGLDILVVRELTGGIYFGPRGIVAIDYGFKAYDTE
VYQTSEIERIAVIAFEAALKRKKKVTSVDKANILDSSRLWRKTVEEISKRYSDVELNHMYVDNCAMQLIKNPSQFDVILT
SNMFGDILSDEASQLTGSIGMLPSASLREDKVGLYEPIHGSAPDIAGTKKANPLATILSVAMMLEYSFNLLDAANDIKDA
VNKVLEAGYRTFDLGKGIILNTQQMGDKVVEYIKG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=342, Percent_Identity=34.2105263157895, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI28178821, Length=346, Percent_Identity=27.7456647398844, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178816, Length=342, Percent_Identity=27.4853801169591, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI4758582, Length=323, Percent_Identity=28.1733746130031, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=28.1733746130031, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI28178819, Length=171, Percent_Identity=34.5029239766082, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=56.6197183098592, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=35.8126721763085, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787381, Length=405, Percent_Identity=26.9135802469136, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=344, Percent_Identity=30.2325581395349, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=32.7635327635328, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=348, Percent_Identity=31.6091954022989, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=31.0650887573964, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=50.4132231404959, Blast_Score=310, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6322097, Length=375, Percent_Identity=36.5333333333333, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=342, Percent_Identity=32.4561403508772, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=347, Percent_Identity=31.9884726224784, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=31.4121037463977, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=31.4121037463977, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24661184, Length=348, Percent_Identity=28.448275862069, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281362242, Length=355, Percent_Identity=30.4225352112676, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648872, Length=355, Percent_Identity=30.4225352112676, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=28.7390029325513, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=28.7390029325513, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=28.7390029325513, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161078637, Length=341, Percent_Identity=28.7390029325513, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=250, Percent_Identity=31.2, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20130355, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38935; Mature: 38935

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIAVLPGDGIGPEVIEEGLKVLSAIEDKYKLKFDVKKYPFGGEAIDKYGVPYPEETKN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHCCCCCCHHHHH
ACLSSDAVLLGAVGGPKWDDLEGDKRPEAGLLALRKSLGVYANLRPAVLYPPLKEASPLK
HHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCEEECCCCCCCCHHH
NELLQEGLDILVVRELTGGIYFGPRGIVAIDYGFKAYDTEVYQTSEIERIAVIAFEAALK
HHHHHCCCCEEEEEECCCCEEECCCCEEEEECCCEECCCHHHCCCCHHHHHHHHHHHHHH
RKKKVTSVDKANILDSSRLWRKTVEEISKRYSDVELNHMYVDNCAMQLIKNPSQFDVILT
HHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCEEEEEE
SNMFGDILSDEASQLTGSIGMLPSASLREDKVGLYEPIHGSAPDIAGTKKANPLATILSV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AMMLEYSFNLLDAANDIKDAVNKVLEAGYRTFDLGKGIILNTQQMGDKVVEYIKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKIAVLPGDGIGPEVIEEGLKVLSAIEDKYKLKFDVKKYPFGGEAIDKYGVPYPEETKN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHCCCCCCHHHHH
ACLSSDAVLLGAVGGPKWDDLEGDKRPEAGLLALRKSLGVYANLRPAVLYPPLKEASPLK
HHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCEEECCCCCCCCHHH
NELLQEGLDILVVRELTGGIYFGPRGIVAIDYGFKAYDTEVYQTSEIERIAVIAFEAALK
HHHHHCCCCEEEEEECCCCEEECCCCEEEEECCCEECCCHHHCCCCHHHHHHHHHHHHHH
RKKKVTSVDKANILDSSRLWRKTVEEISKRYSDVELNHMYVDNCAMQLIKNPSQFDVILT
HHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCEEEEEE
SNMFGDILSDEASQLTGSIGMLPSASLREDKVGLYEPIHGSAPDIAGTKKANPLATILSV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AMMLEYSFNLLDAANDIKDAVNKVLEAGYRTFDLGKGIILNTQQMGDKVVEYIKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]