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The map label for this gene is potD [C]

Identifier: 163845212

GI number: 163845212

Start: 1079599

End: 1080681

Strand: Reverse

Name: potD [C]

Synonym: BSUIS_B1099

Alternate gene names: 163845212

Gene position: 1080681-1079599 (Counterclockwise)

Preceding gene: 163845213

Following gene: 163845198

Centisome position: 77.14

GC content: 55.59

Gene sequence:

>1083_bases
ATGATTGAAATAAAAAATAGAAGAGAGGGAAACATGTCCAAGAAAATAAAAACGATGACTGTGCTGGCCGCGGTTCTGCT
GGCTGGCGGTGCGCTTGCCGCAAAAGCCGATAGCATCGTCTACGCCACGACTGGCGGACAGATGCGCCAGACGCTTGAAC
GCGCATTCTACCAGCCTTTTGCAAAGGAAAGCGGCACGGACGTCATCCCATATGACATCGAAGTGCCCGACCAGTGGGCC
CGTGTCGAAGCGATGAAGCGGGCCGGAAAATACGATTTCGATATCGTTACGGCAACCGGCCCGGACCTGGTCGACAAGGC
CGACCTTCTTTTGACCATCGATTGTTCGAAACTGCCCAATGTGGAAGCGAAGGGCCTTGAGGGTTCCTGCAAGGAAAAAG
GCGTGGCCCGGACCACCGGCGGCATGCTGCTCGCCTATGATGCATCCGTGTATAAAGACAAGGTTTCGCAAACATGGGCT
GATTTTTGGGATGTGAAGACATTTCCCGGCCCGCGTGGCCTGCCCGATACCGGCGATCGCGACTGGTGGCTGCCGGCCAT
TGCCTTGATGGCGGACGGCGTGCCCCGCGACAAGATTTTTCCGATGGACCTTGATCGCGCCTACAAGAAGCTCGATGAGA
TCAAGCCCTATATCAGCGTGTGGTGGAAGACCGGCAATCAGGTTGCGCAGATCATGCGCGATCAGGAAGTGGCTATGACC
CTGTCCTATTCCGGGCGCGCCTTGACCGCGATGAACGAAGGCGCTCCCTTCCAGCTGAGCTGGAACGACGCCATCATGGA
TACGGGATATTTCGCCATTCTCAAGGATGCGCCAAATCCGGAAGGCGCCCTGAAATTCATCAATTATTTCTACGGTCACG
CCGAAGGGCACCCGGCCTTCATCAAGACTGCAAACTATGCGACCCATTCCAAGGATGGTATGGCGTTGCTTCCGCCGGAA
GAACAGAAGAATTATGCGACGGCGCCTGAGAATTTCAAACTGCTGATGGTGCCGGACTTCAAGTGGATCGGTGAAAACCG
CGACATGCTTCGCGAACGCTGGCAAAACTGGCTGGCCGAATGA

Upstream 100 bases:

>100_bases
CCGGCTATCGCGGTGATGCTGACGCTGCTGTCCATCCTCGTGCTGGTCGGCGCCGCCGGTCTTCGCACGCTGGCCGCGCG
CTCTCAAAAAGTAGTGTCCG

Downstream 100 bases:

>100_bases
TCGGCAAGAAAGCCGGCTTCGTTTGTGGAAGCCGGCTTTTGTCTTTTATGCGGCAAGCATCGGCGGTTTCCTTTGCGGCC
ACAAGGTCGCGCGGTGATAG

Product: hypothetical protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: Twin-Arginine Translocation Pathway Signal; Polyamine ABC Transporter; Spermidine/Putrescine ABC Transporter; Spermidine/Putrescine ABC Transporter Periplasmic Protein; Tat Pathway Signal Sequence Domain-Containing Protein; ABC Transporter; Spermidine/Putrescine ABC Transpoter; Polyamine ABC Transporter Exported Protein; Polyamine ABC Transporter Periplasmic Protein; ABC Transporter Periplasmic Protein; PotD-A

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MIEIKNRREGNMSKKIKTMTVLAAVLLAGGALAAKADSIVYATTGGQMRQTLERAFYQPFAKESGTDVIPYDIEVPDQWA
RVEAMKRAGKYDFDIVTATGPDLVDKADLLLTIDCSKLPNVEAKGLEGSCKEKGVARTTGGMLLAYDASVYKDKVSQTWA
DFWDVKTFPGPRGLPDTGDRDWWLPAIALMADGVPRDKIFPMDLDRAYKKLDEIKPYISVWWKTGNQVAQIMRDQEVAMT
LSYSGRALTAMNEGAPFQLSWNDAIMDTGYFAILKDAPNPEGALKFINYFYGHAEGHPAFIKTANYATHSKDGMALLPPE
EQKNYATAPENFKLLMVPDFKWIGENRDMLRERWQNWLAE

Sequences:

>Translated_360_residues
MIEIKNRREGNMSKKIKTMTVLAAVLLAGGALAAKADSIVYATTGGQMRQTLERAFYQPFAKESGTDVIPYDIEVPDQWA
RVEAMKRAGKYDFDIVTATGPDLVDKADLLLTIDCSKLPNVEAKGLEGSCKEKGVARTTGGMLLAYDASVYKDKVSQTWA
DFWDVKTFPGPRGLPDTGDRDWWLPAIALMADGVPRDKIFPMDLDRAYKKLDEIKPYISVWWKTGNQVAQIMRDQEVAMT
LSYSGRALTAMNEGAPFQLSWNDAIMDTGYFAILKDAPNPEGALKFINYFYGHAEGHPAFIKTANYATHSKDGMALLPPE
EQKNYATAPENFKLLMVPDFKWIGENRDMLRERWQNWLAE
>Mature_360_residues
MIEIKNRREGNMSKKIKTMTVLAAVLLAGGALAAKADSIVYATTGGQMRQTLERAFYQPFAKESGTDVIPYDIEVPDQWA
RVEAMKRAGKYDFDIVTATGPDLVDKADLLLTIDCSKLPNVEAKGLEGSCKEKGVARTTGGMLLAYDASVYKDKVSQTWA
DFWDVKTFPGPRGLPDTGDRDWWLPAIALMADGVPRDKIFPMDLDRAYKKLDEIKPYISVWWKTGNQVAQIMRDQEVAMT
LSYSGRALTAMNEGAPFQLSWNDAIMDTGYFAILKDAPNPEGALKFINYFYGHAEGHPAFIKTANYATHSKDGMALLPPE
EQKNYATAPENFKLLMVPDFKWIGENRDMLRERWQNWLAE

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40362; Mature: 40362

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKNRREGNMSKKIKTMTVLAAVLLAGGALAAKADSIVYATTGGQMRQTLERAFYQPF
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCEEEEECCHHHHHHHHHHHHCCH
AKESGTDVIPYDIEVPDQWARVEAMKRAGKYDFDIVTATGPDLVDKADLLLTIDCSKLPN
HHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCC
VEAKGLEGSCKEKGVARTTGGMLLAYDASVYKDKVSQTWADFWDVKTFPGPRGLPDTGDR
CCCCCCCCCHHHCCCEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DWWLPAIALMADGVPRDKIFPMDLDRAYKKLDEIKPYISVWWKTGNQVAQIMRDQEVAMT
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHEEEECCCHHHHHHHCCCCEEEE
LSYSGRALTAMNEGAPFQLSWNDAIMDTGYFAILKDAPNPEGALKFINYFYGHAEGHPAF
EECCCEEEEEECCCCCEEEECCCHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEE
IKTANYATHSKDGMALLPPEEQKNYATAPENFKLLMVPDFKWIGENRDMLRERWQNWLAE
EEECCCCCCCCCCEEECCCHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEIKNRREGNMSKKIKTMTVLAAVLLAGGALAAKADSIVYATTGGQMRQTLERAFYQPF
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCEEEEECCHHHHHHHHHHHHCCH
AKESGTDVIPYDIEVPDQWARVEAMKRAGKYDFDIVTATGPDLVDKADLLLTIDCSKLPN
HHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCC
VEAKGLEGSCKEKGVARTTGGMLLAYDASVYKDKVSQTWADFWDVKTFPGPRGLPDTGDR
CCCCCCCCCHHHCCCEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DWWLPAIALMADGVPRDKIFPMDLDRAYKKLDEIKPYISVWWKTGNQVAQIMRDQEVAMT
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHEEEECCCHHHHHHHCCCCEEEE
LSYSGRALTAMNEGAPFQLSWNDAIMDTGYFAILKDAPNPEGALKFINYFYGHAEGHPAF
EECCCEEEEEECCCCCEEEECCCHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEE
IKTANYATHSKDGMALLPPEEQKNYATAPENFKLLMVPDFKWIGENRDMLRERWQNWLAE
EEECCCCCCCCCCEEECCCHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA