BacMap

Definition	Brucella suis ATCC 23445 chromosome II, complete genome.
Accession	NC_010167
Length	1,400,844

Click here to switch to the map view.

The map label for this gene is echA8 [H]

Identifier: 163845198

GI number: 163845198

Start: 1060595

End: 1061443

Strand: Reverse

Name: echA8 [H]

Synonym: BSUIS_B1083

Alternate gene names: 163845198

Gene position: 1061443-1060595 (Counterclockwise)

Preceding gene: 163845212

Following gene: 163845197

Centisome position: 75.77

GC content: 56.42

Gene sequence:

>849_bases
ATGAAATTTAATTCCATTACTGGAGGTTCCACGTCGGGTACGATGTGGGACGGCATGGGGAACAAAATGAGCGAAGCTAC
TGATGTTGTGATAGAAACTCGTCCGGCTGACGGTGTTGCCCTCCTGGAACTCAACCGCCCTGATGCATTGAATGCCGTGA
ACATGGATGTGCGCCAGAAACTGGCTGCATCAGCCGACAGTCTCGTTGAGGATCCGGACATCCGGGTGATCGTCATTGCC
GGACGTGGCGGAAATTTCGCCGCGGGTTCAGATGTCAAGGTTTTTGCGCAAACCGGGGCGGGTAGTCTGCTTGCACAGCG
CATGCACCGCTATTGGGAAAGCCTCGCCCATTGCCCCAAGCCGGTTATCGCGGCCGTAGAGGGATATGCCCTAGGTGGCG
GGTGCGAGCTGGCAATGCACGCCGACATTATTGTAGCTGCAAGAACCGCCAGTTTCGGCCAGCCAGAAATAAAGCTCGGA
CTGATGCCTGGAGCAGGCGGCACGCAGCGCCTTTTGCGCGCGATCGGCAAGTACAAGACTATGCTGCTCGCATTGACGGG
CGAAATGCTGCCTGCAACGGAAGCTGAAAAATACGGACTTGTAAGCAGGCTCTCGGAAGAAGGTGAAGCGCTTGAAGAAG
CCTTGAAACTGGCGCGGAAAATCGCGCTTATGCCCGCGTTGGCTGCTGAACAGATAAAGGAGGCGGTCATGTACGGCGAG
GATGCGCCGCTGGAAACCGCACTCCGCCTGGAGCGCAAGGCATTTCAGCTCCTGTTCGATACGGAAGACAAGCGTGAAGG
CATCGATGCATTTTTGACCAAGCGCAAGCCGGCATTCAAAGGGCGCTGA

Upstream 100 bases:

>100_bases
ATCGAACAGTTTCCTGCCCGGGGCGGAAACGCTTTTCGATTGCTGAAATCTCCTATTGCTAAGCGAATGTTTCATAGAAT
ATTGTTCAACTCAACATAAA

Downstream 100 bases:

>100_bases
AATGGTCATTGAAACAGTGGCTTATGGTCAGATCGCAAAAGTCGCGATCATCGGCTCCGGTGTCATGGGAGCAGGCATCG
CCGAGACGATGGCAGCTGGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKFNSITGGSTSGTMWDGMGNKMSEATDVVIETRPADGVALLELNRPDALNAVNMDVRQKLAASADSLVEDPDIRVIVIA
GRGGNFAAGSDVKVFAQTGAGSLLAQRMHRYWESLAHCPKPVIAAVEGYALGGGCELAMHADIIVAARTASFGQPEIKLG
LMPGAGGTQRLLRAIGKYKTMLLALTGEMLPATEAEKYGLVSRLSEEGEALEEALKLARKIALMPALAAEQIKEAVMYGE
DAPLETALRLERKAFQLLFDTEDKREGIDAFLTKRKPAFKGR

Sequences:

>Translated_282_residues
MKFNSITGGSTSGTMWDGMGNKMSEATDVVIETRPADGVALLELNRPDALNAVNMDVRQKLAASADSLVEDPDIRVIVIA
GRGGNFAAGSDVKVFAQTGAGSLLAQRMHRYWESLAHCPKPVIAAVEGYALGGGCELAMHADIIVAARTASFGQPEIKLG
LMPGAGGTQRLLRAIGKYKTMLLALTGEMLPATEAEKYGLVSRLSEEGEALEEALKLARKIALMPALAAEQIKEAVMYGE
DAPLETALRLERKAFQLLFDTEDKREGIDAFLTKRKPAFKGR
>Mature_282_residues
MKFNSITGGSTSGTMWDGMGNKMSEATDVVIETRPADGVALLELNRPDALNAVNMDVRQKLAASADSLVEDPDIRVIVIA
GRGGNFAAGSDVKVFAQTGAGSLLAQRMHRYWESLAHCPKPVIAAVEGYALGGGCELAMHADIIVAARTASFGQPEIKLG
LMPGAGGTQRLLRAIGKYKTMLLALTGEMLPATEAEKYGLVSRLSEEGEALEEALKLARKIALMPALAAEQIKEAVMYGE
DAPLETALRLERKAFQLLFDTEDKREGIDAFLTKRKPAFKGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=42.6229508196721, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=35.4581673306773, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI31542718, Length=266, Percent_Identity=30.8270676691729, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI68989263, Length=278, Percent_Identity=28.4172661870504, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI70995211, Length=244, Percent_Identity=31.5573770491803, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=33.1428571428571, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI37594469, Length=206, Percent_Identity=31.5533980582524, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI37594471, Length=206, Percent_Identity=31.5533980582524, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=30.1960784313725, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=223, Percent_Identity=26.9058295964126, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI157694516, Length=223, Percent_Identity=26.9058295964126, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI45643119, Length=245, Percent_Identity=23.265306122449, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI260275230, Length=245, Percent_Identity=23.265306122449, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI260274832, Length=245, Percent_Identity=23.265306122449, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI157694520, Length=183, Percent_Identity=27.8688524590164, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=45.4918032786885, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=38.2470119521912, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=35.1778656126482, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=30.0751879699248, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=29.8804780876494, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790281, Length=287, Percent_Identity=27.5261324041812, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=44.2231075697211, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=40.4858299595142, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=34.3220338983051, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=31.496062992126, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=239, Percent_Identity=30.9623430962343, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=227, Percent_Identity=29.5154185022026, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=32.9479768786127, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=200, Percent_Identity=32.5, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=200, Percent_Identity=32.5, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=200, Percent_Identity=32.5, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=28.3950617283951, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=239, Percent_Identity=27.6150627615063, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=22.7272727272727, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=25.2577319587629, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=44.7154471544715, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=44.7154471544715, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=32.089552238806, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19920382, Length=264, Percent_Identity=31.4393939393939, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24650670, Length=246, Percent_Identity=28.8617886178862, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922422, Length=268, Percent_Identity=25.7462686567164, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357171, Length=248, Percent_Identity=27.8225806451613, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571730, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654903, Length=243, Percent_Identity=25.5144032921811, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583077, Length=183, Percent_Identity=29.5081967213115, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583079, Length=183, Percent_Identity=29.5081967213115, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921000, Length=183, Percent_Identity=29.5081967213115, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 30192; Mature: 30192

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNSITGGSTSGTMWDGMGNKMSEATDVVIETRPADGVALLELNRPDALNAVNMDVRQK
CCCCCCCCCCCCCCEECCCCCHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHH
LAASADSLVEDPDIRVIVIAGRGGNFAAGSDVKVFAQTGAGSLLAQRMHRYWESLAHCPK
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
PVIAAVEGYALGGGCELAMHADIIVAARTASFGQPEIKLGLMPGAGGTQRLLRAIGKYKT
HHHHHHCCEEECCCCEEEEECEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
MLLALTGEMLPATEAEKYGLVSRLSEEGEALEEALKLARKIALMPALAAEQIKEAVMYGE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAPLETALRLERKAFQLLFDTEDKREGIDAFLTKRKPAFKGR
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKFNSITGGSTSGTMWDGMGNKMSEATDVVIETRPADGVALLELNRPDALNAVNMDVRQK
CCCCCCCCCCCCCCEECCCCCHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHH
LAASADSLVEDPDIRVIVIAGRGGNFAAGSDVKVFAQTGAGSLLAQRMHRYWESLAHCPK
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
PVIAAVEGYALGGGCELAMHADIIVAARTASFGQPEIKLGLMPGAGGTQRLLRAIGKYKT
HHHHHHCCEEECCCCEEEEECEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
MLLALTGEMLPATEAEKYGLVSRLSEEGEALEEALKLARKIALMPALAAEQIKEAVMYGE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAPLETALRLERKAFQLLFDTEDKREGIDAFLTKRKPAFKGR
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]