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The map label for this gene is nikB [H]

Identifier: 163844928

GI number: 163844928

Start: 773636

End: 774580

Strand: Reverse

Name: nikB [H]

Synonym: BSUIS_B0795

Alternate gene names: 163844928

Gene position: 774580-773636 (Counterclockwise)

Preceding gene: 163844929

Following gene: 163844927

Centisome position: 55.29

GC content: 59.26

Gene sequence:

>945_bases
GTGTTCGCCTTCATTATCCGCCGGGTGATCCTGCTTGTCCCGATGTTGTTCGGGGCATCGCTGGTCATTTTCCTGATGCT
GCGGCTTGGCCCGAGCGACCCCGCCATGGATTATCTGCGCCTGTCGAAAGTGCCTCCAACGCCGCAGGCGTTGGAGAGCG
CCCGCCAGATGCTCGGGCTGGATCGGGCAATTACGACCCAGTATTTCGACTGGCTTGGAAAGGCGCTCCATGGTGATTTC
GGGATATCGTTTGCGACCCAACGGCCGGTCCTGCCGGATTTGCTCTACTTCTTGCCGGCCACGCTTCAGCTTGCAGGTGT
CGCGCTTGTGCTGACACTCGGCCTGTCCATTCCGCTCGGTATGTGGGCGGCCCGACACCGGGAGGGCTGGCAGGATCAGG
TGGTCCGGTTTGTTGCCTTCCTTGGCGTATCCATGCCCAACTTCTGGCTCGGCTTCCTGTTGGTTCTGGTGTTTTCCATC
CAGCTTGGCTGGTTGCCGCCAATGGGGCGCGGCGGCTTTGCGCATATGATTATGCCAGCCATCGCGATTTCCTTCATGTC
GCTCTCCATCAATGCGCGCTTGCTGAGGGCCAGTATGCTGGAAGTGTCAGGACAAAGGCATGTGCGTTATGCCCGCCTGC
GCGGCCTGTCTGAGCGCAGTGTAGAGCGCAGCCATGTGCTGAGAAATGCCTGGCTGCCGATCATCACGGCAACGGGGATG
CATATCGGTGAACTGATCGGCGGTACACTGGTCATCGAAAGCATTTTTGGCTGGCCGGGCGTCGGGCGCTATGCCGTCTC
GGCCATCCTGAACCGCGACTATCCCGTTATCCAGTGTTTTACGCTGGTTATGGTGACGATCTTTGTCATCTGCAATCTGA
TCGTGGATGTCGTTTATGCCTGGGCCGATCCGCGCATCCGCCTTTCTGCGGAGGGTGCGGAATGA

Upstream 100 bases:

>100_bases
ACCGCGATGGCGGTTGCGAAACCTGAGGTCGGCAAAATCACATTCGGAGCGATGTCGAGCGAGATCCCCTTTGAGAAACT
GACCCCGAAGGCGAACTGAG

Downstream 100 bases:

>100_bases
GTGATTCAGCTTCCATGGTTTGTGTCTCGAAGACGGGCTTTCTGTCGGTTCCGCGGCAGTGGCCGGTTCGTCTTGCCTGC
ATCATTGTCCTGTTATTATT

Product: nickel transporter permease NikB

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MFAFIIRRVILLVPMLFGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPQALESARQMLGLDRAITTQYFDWLGKALHGDF
GISFATQRPVLPDLLYFLPATLQLAGVALVLTLGLSIPLGMWAARHREGWQDQVVRFVAFLGVSMPNFWLGFLLVLVFSI
QLGWLPPMGRGGFAHMIMPAIAISFMSLSINARLLRASMLEVSGQRHVRYARLRGLSERSVERSHVLRNAWLPIITATGM
HIGELIGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLVMVTIFVICNLIVDVVYAWADPRIRLSAEGAE

Sequences:

>Translated_314_residues
MFAFIIRRVILLVPMLFGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPQALESARQMLGLDRAITTQYFDWLGKALHGDF
GISFATQRPVLPDLLYFLPATLQLAGVALVLTLGLSIPLGMWAARHREGWQDQVVRFVAFLGVSMPNFWLGFLLVLVFSI
QLGWLPPMGRGGFAHMIMPAIAISFMSLSINARLLRASMLEVSGQRHVRYARLRGLSERSVERSHVLRNAWLPIITATGM
HIGELIGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLVMVTIFVICNLIVDVVYAWADPRIRLSAEGAE
>Mature_314_residues
MFAFIIRRVILLVPMLFGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPQALESARQMLGLDRAITTQYFDWLGKALHGDF
GISFATQRPVLPDLLYFLPATLQLAGVALVLTLGLSIPLGMWAARHREGWQDQVVRFVAFLGVSMPNFWLGFLLVLVFSI
QLGWLPPMGRGGFAHMIMPAIAISFMSLSINARLLRASMLEVSGQRHVRYARLRGLSERSVERSHVLRNAWLPIITATGM
HIGELIGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLVMVTIFVICNLIVDVVYAWADPRIRLSAEGAE

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=314, Percent_Identity=68.7898089171974, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=38.6363636363636, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=34.9112426035503, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=35.4838709677419, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1787761, Length=329, Percent_Identity=35.258358662614, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=35.0943396226415, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788504, Length=364, Percent_Identity=23.6263736263736, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34921; Mature: 34921

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAFIIRRVILLVPMLFGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPQALESARQMLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
DRAITTQYFDWLGKALHGDFGISFATQRPVLPDLLYFLPATLQLAGVALVLTLGLSIPLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWAARHREGWQDQVVRFVAFLGVSMPNFWLGFLLVLVFSIQLGWLPPMGRGGFAHMIMPA
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IAISFMSLSINARLLRASMLEVSGQRHVRYARLRGLSERSVERSHVLRNAWLPIITATGM
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHCCH
HIGELIGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLVMVTIFVICNLIVDVVYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WADPRIRLSAEGAE
CCCCCEEEECCCCC
>Mature Secondary Structure
MFAFIIRRVILLVPMLFGASLVIFLMLRLGPSDPAMDYLRLSKVPPTPQALESARQMLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
DRAITTQYFDWLGKALHGDFGISFATQRPVLPDLLYFLPATLQLAGVALVLTLGLSIPLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWAARHREGWQDQVVRFVAFLGVSMPNFWLGFLLVLVFSIQLGWLPPMGRGGFAHMIMPA
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IAISFMSLSINARLLRASMLEVSGQRHVRYARLRGLSERSVERSHVLRNAWLPIITATGM
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHCCH
HIGELIGGTLVIESIFGWPGVGRYAVSAILNRDYPVIQCFTLVMVTIFVICNLIVDVVYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WADPRIRLSAEGAE
CCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]