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The map label for this gene is nikC [H]

Identifier: 163844927

GI number: 163844927

Start: 772767

End: 773639

Strand: Reverse

Name: nikC [H]

Synonym: BSUIS_B0794

Alternate gene names: 163844927

Gene position: 773639-772767 (Counterclockwise)

Preceding gene: 163844928

Following gene: 163844926

Centisome position: 55.23

GC content: 60.48

Gene sequence:

>873_bases
ATGAGTGATTCAGCTTCCATGGTTTGTGTCTCGAAGACGGGCTTTCTGTCGGTTCCGCGGCAGTGGCCGGTTCGTCTTGC
CTGCATCATTGTCCTGTTATTATTGGTCGCGGCTGTCGCTGGCCCCTGGATTACCCCTTATAACCCGGATGATGTCGATC
TGGTGCGCCGGCTTCAATCACCAAGCCTTGCCCATTGGCTGGGCACCGATCACCTTGGCCGCGACATGCTCTCACGCCTG
ATCGCAGGTGTGCGGGTATCGCTCGGTTCTGTTGTCATCACCCTGGCGCTGATCCTGGCCATGGGAATTGTCATCGGGGG
CGCGGCGGGCTTTCTCGGCGGGCGGGCGGATCAGGTCATCATGCGCATCACCGATGTGTTTCTCACCTTTCCAACGCTGG
TGCTGGCTCTGTTCATGGTCGGTATGCTCGGGACGGGGCTGGTGAATCTCATACTTGCCATCGCCTTGTCGCACTGGGCC
TGGTATGCGCGGATCGTACGCGGCATCGTAATGTCGCTCAGGCATCGGGAGTTTCTGCTTGCCGCGCGGATGAGCGGCGC
GGGTCCGGTCCGCACCTTCTGGGAGCATCTTCTGCCTACAACCTTTTCACAGCTTGTCGTCCTGGCAACCCTTGATGTCG
GGCATATCATGCTCCACGTCTCGGGCCTGTCTTTTCTCGGGCTTGGCGTCACGCCGCCGACCGCGGAATGGGGCGTGATG
ATCAACGATGCCCGCCAGTTCGTCTGGACCGCACCGATGCTGATCCTGTGGCCGGGGCTGGCGCTGTTTTTGAGCGTCAT
GGCCTTCAACATTCTGGGGGACGCCTTGCGTGACAGCCTCGACCCGCATCTCAAGGCGGAGCACAGCCATTGA

Upstream 100 bases:

>100_bases
CGCTGGTTATGGTGACGATCTTTGTCATCTGCAATCTGATCGTGGATGTCGTTTATGCCTGGGCCGATCCGCGCATCCGC
CTTTCTGCGGAGGGTGCGGA

Downstream 100 bases:

>100_bases
CCAGGAAGACACTCGCCATTGAAGGCCTGACCGCGACCACCGTGATAGACGGGCAGCAGCGCGTCCTTGTCGATAATCTT
TCTCTTGGCGTTCAACGCGG

Product: nickel transporter permease NikC

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSDSASMVCVSKTGFLSVPRQWPVRLACIIVLLLLVAAVAGPWITPYNPDDVDLVRRLQSPSLAHWLGTDHLGRDMLSRL
IAGVRVSLGSVVITLALILAMGIVIGGAAGFLGGRADQVIMRITDVFLTFPTLVLALFMVGMLGTGLVNLILAIALSHWA
WYARIVRGIVMSLRHREFLLAARMSGAGPVRTFWEHLLPTTFSQLVVLATLDVGHIMLHVSGLSFLGLGVTPPTAEWGVM
INDARQFVWTAPMLILWPGLALFLSVMAFNILGDALRDSLDPHLKAEHSH

Sequences:

>Translated_290_residues
MSDSASMVCVSKTGFLSVPRQWPVRLACIIVLLLLVAAVAGPWITPYNPDDVDLVRRLQSPSLAHWLGTDHLGRDMLSRL
IAGVRVSLGSVVITLALILAMGIVIGGAAGFLGGRADQVIMRITDVFLTFPTLVLALFMVGMLGTGLVNLILAIALSHWA
WYARIVRGIVMSLRHREFLLAARMSGAGPVRTFWEHLLPTTFSQLVVLATLDVGHIMLHVSGLSFLGLGVTPPTAEWGVM
INDARQFVWTAPMLILWPGLALFLSVMAFNILGDALRDSLDPHLKAEHSH
>Mature_289_residues
SDSASMVCVSKTGFLSVPRQWPVRLACIIVLLLLVAAVAGPWITPYNPDDVDLVRRLQSPSLAHWLGTDHLGRDMLSRLI
AGVRVSLGSVVITLALILAMGIVIGGAAGFLGGRADQVIMRITDVFLTFPTLVLALFMVGMLGTGLVNLILAIALSHWAW
YARIVRGIVMSLRHREFLLAARMSGAGPVRTFWEHLLPTTFSQLVVLATLDVGHIMLHVSGLSFLGLGVTPPTAEWGVMI
NDARQFVWTAPMLILWPGLALFLSVMAFNILGDALRDSLDPHLKAEHSH

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789889, Length=276, Percent_Identity=64.8550724637681, Blast_Score=340, Evalue=4e-95,
Organism=Escherichia coli, GI1787054, Length=267, Percent_Identity=42.3220973782771, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787760, Length=263, Percent_Identity=37.2623574144487, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1789964, Length=257, Percent_Identity=40.8560311284047, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1787498, Length=259, Percent_Identity=33.2046332046332, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1787549, Length=254, Percent_Identity=33.8582677165354, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788505, Length=229, Percent_Identity=27.5109170305677, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014157 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31481; Mature: 31350

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SDSASMVCVSKTGFLSVPRQWPVRLACIIVLLLLVAAVAGPWITPYNPDDVDLVRRLQS
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
PSLAHWLGTDHLGRDMLSRLIAGVRVSLGSVVITLALILAMGIVIGGAAGFLGGRADQVI
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MRITDVFLTFPTLVLALFMVGMLGTGLVNLILAIALSHWAWYARIVRGIVMSLRHREFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARMSGAGPVRTFWEHLLPTTFSQLVVLATLDVGHIMLHVSGLSFLGLGVTPPTAEWGVM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCHH
INDARQFVWTAPMLILWPGLALFLSVMAFNILGDALRDSLDPHLKAEHSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]