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The map label for this gene is nikA [H]

Identifier: 163844929

GI number: 163844929

Start: 774582

End: 776162

Strand: Reverse

Name: nikA [H]

Synonym: BSUIS_B0796

Alternate gene names: 163844929

Gene position: 776162-774582 (Counterclockwise)

Preceding gene: 163844931

Following gene: 163844928

Centisome position: 55.41

GC content: 54.9

Gene sequence:

>1581_bases
ATGTTGACGGATAGGTTGCGCGGGCTGATGCTCGCATTTGCTTTTGTTTCTGTAGCGTTTGTTTCCGGCGCTCATGCAGA
TCCAAAGCTGAATTTTTCCTGGCCGGTGAATGTAGGGCCGCTCAATCCGCACCTTTATTCTCCGAACCAGATGTTTGCCC
AGAACATGGTTTACGAGCCGCTGGTTCATTATAATGCTGACGGCACCGTTGGCCCCTGGCTGGCAGAATCCTGGGAAGCG
TCTCAGGATGGCCGATCCTACACATTCAAGCTTCGGGAGGACGTCAAGTTCTCGAATGGCGAGGTTTTCGATGCGGCTGC
GGTCAAGGCAAATATTGATACCGTATTGCAAAACCGCCCACGTCATAACTGGCTCGAACTTGTTAACCAGATGGTCTCTG
CCGAGGTTGTCGGTCCTTACAAGGTGCGGATTAATCTCAAAAAACCTTATTACCCGTTATTGCAGGAGCTTTCCCTGCCG
CGCCCCTTCCGCTTCATTGCCCCGTCGCAGTTCAAAAATGGCGGCACCGCAGATGGCATTGTGGCGCCGATTGGTACGGG
CCCGTGGAAATTGACCGAGACCAAACTTGGCGAGCACGACGTATTTACCCGCAATGATAGCTACTGGGGTCCCAAGCCTG
CTTATGAGCAGATAACTGTCAAGGTGATACCCGATCCGAATACACGGGCGATTGCCTTCGAGGCTGGCGAAATAGACCTG
ATCTATGGAACGGAAGGGCCGATTTCCCCCGATACTTTCGAGCGCTTCCAGAAAATGGGCATCTATAATACCGAGCTTTC
GGAGCCGTTGGAAACGCGCGTTTTGGCGCTCAACACCAATCACGGCGCAACAAAAGACCTCGCTGTCCGCAAGGCCATCA
ATCATGCAGTCGATAAGGACACGATGATTGCCACCGTGCTCTACGGGACACAGAAGCGTGCAGATACCCTGTTTGCCGAT
AATGTTCCCTATGCCAATATCGGCCTGAAGCCTTATGCTTTCGACCCGGCTCTGGCTGCCCGGCTTCTGGATGAAGCCGG
ATGGACAGCCAAGGCAAGCGGCGATATCCGGGAGAAGGATGGGCAGCCTCTGGCGATCGAGTTGTGCTTCATCGGCACGG
ATGCGATCAGCAAATCGATGGCCGAGATCGTGCAGGCGGATCTGCGTAAGGTCGGTATCGATGTCAAGCTGACGGGCGAG
GAGGAAAGCAGCATCTATGCTCGCCAGCGCGATGGTCGTTTCGATATGATTTTCAACCAGACCTGGGGTGCGCCCTACGA
TCCGCACGCTTTTGTCAGCTCCATGCGCGTTCCCTCCCATGCCGATTATCAGGCGCAGCTCGGCCTGCCGGACAAGGCGA
AAATCGATGCGGAGATCGGTCAGGTTCTTGTCTCGACTGATGAGACGGCAAGGCAGGCGCTTTACAAGGATATCCTGACC
CGTCTTCACGAGGAAGCGGTTTACCTTCCGCTCACCTCTGTCACCGCGATGGCGGTTGCGAAACCTGAGGTCGGCAAAAT
CACATTCGGAGCGATGTCGAGCGAGATCCCCTTTGAGAAACTGACCCCGAAGGCGAACTGA

Upstream 100 bases:

>100_bases
TGCGCTCTAATGAAATTGACATGTTGCCGTTGACTCTGACTGTATATGATGTTTTTAGCAAATAATCATACTTACTACTT
GATTCATTAGAGGCTTATAG

Downstream 100 bases:

>100_bases
GGTGTTCGCCTTCATTATCCGCCGGGTGATCCTGCTTGTCCCGATGTTGTTCGGGGCATCGCTGGTCATTTTCCTGATGC
TGCGGCTTGGCCCGAGCGAC

Product: nickel ABC transporter, periplasmic nickel-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MLTDRLRGLMLAFAFVSVAFVSGAHADPKLNFSWPVNVGPLNPHLYSPNQMFAQNMVYEPLVHYNADGTVGPWLAESWEA
SQDGRSYTFKLREDVKFSNGEVFDAAAVKANIDTVLQNRPRHNWLELVNQMVSAEVVGPYKVRINLKKPYYPLLQELSLP
RPFRFIAPSQFKNGGTADGIVAPIGTGPWKLTETKLGEHDVFTRNDSYWGPKPAYEQITVKVIPDPNTRAIAFEAGEIDL
IYGTEGPISPDTFERFQKMGIYNTELSEPLETRVLALNTNHGATKDLAVRKAINHAVDKDTMIATVLYGTQKRADTLFAD
NVPYANIGLKPYAFDPALAARLLDEAGWTAKASGDIREKDGQPLAIELCFIGTDAISKSMAEIVQADLRKVGIDVKLTGE
EESSIYARQRDGRFDMIFNQTWGAPYDPHAFVSSMRVPSHADYQAQLGLPDKAKIDAEIGQVLVSTDETARQALYKDILT
RLHEEAVYLPLTSVTAMAVAKPEVGKITFGAMSSEIPFEKLTPKAN

Sequences:

>Translated_526_residues
MLTDRLRGLMLAFAFVSVAFVSGAHADPKLNFSWPVNVGPLNPHLYSPNQMFAQNMVYEPLVHYNADGTVGPWLAESWEA
SQDGRSYTFKLREDVKFSNGEVFDAAAVKANIDTVLQNRPRHNWLELVNQMVSAEVVGPYKVRINLKKPYYPLLQELSLP
RPFRFIAPSQFKNGGTADGIVAPIGTGPWKLTETKLGEHDVFTRNDSYWGPKPAYEQITVKVIPDPNTRAIAFEAGEIDL
IYGTEGPISPDTFERFQKMGIYNTELSEPLETRVLALNTNHGATKDLAVRKAINHAVDKDTMIATVLYGTQKRADTLFAD
NVPYANIGLKPYAFDPALAARLLDEAGWTAKASGDIREKDGQPLAIELCFIGTDAISKSMAEIVQADLRKVGIDVKLTGE
EESSIYARQRDGRFDMIFNQTWGAPYDPHAFVSSMRVPSHADYQAQLGLPDKAKIDAEIGQVLVSTDETARQALYKDILT
RLHEEAVYLPLTSVTAMAVAKPEVGKITFGAMSSEIPFEKLTPKAN
>Mature_526_residues
MLTDRLRGLMLAFAFVSVAFVSGAHADPKLNFSWPVNVGPLNPHLYSPNQMFAQNMVYEPLVHYNADGTVGPWLAESWEA
SQDGRSYTFKLREDVKFSNGEVFDAAAVKANIDTVLQNRPRHNWLELVNQMVSAEVVGPYKVRINLKKPYYPLLQELSLP
RPFRFIAPSQFKNGGTADGIVAPIGTGPWKLTETKLGEHDVFTRNDSYWGPKPAYEQITVKVIPDPNTRAIAFEAGEIDL
IYGTEGPISPDTFERFQKMGIYNTELSEPLETRVLALNTNHGATKDLAVRKAINHAVDKDTMIATVLYGTQKRADTLFAD
NVPYANIGLKPYAFDPALAARLLDEAGWTAKASGDIREKDGQPLAIELCFIGTDAISKSMAEIVQADLRKVGIDVKLTGE
EESSIYARQRDGRFDMIFNQTWGAPYDPHAFVSSMRVPSHADYQAQLGLPDKAKIDAEIGQVLVSTDETARQALYKDILT
RLHEEAVYLPLTSVTAMAVAKPEVGKITFGAMSSEIPFEKLTPKAN

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=524, Percent_Identity=63.3587786259542, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1787052, Length=496, Percent_Identity=24.3951612903226, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789966, Length=492, Percent_Identity=25.609756097561, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1787762, Length=469, Percent_Identity=22.8144989339019, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787495, Length=504, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082063, Length=397, Percent_Identity=25.1889168765743, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787551, Length=454, Percent_Identity=23.3480176211454, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081878, Length=469, Percent_Identity=24.3070362473348, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789397, Length=477, Percent_Identity=22.2222222222222, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58244; Mature: 58244

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDRLRGLMLAFAFVSVAFVSGAHADPKLNFSWPVNVGPLNPHLYSPNQMFAQNMVYEP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCHHHHHHHHHHCC
LVHYNADGTVGPWLAESWEASQDGRSYTFKLREDVKFSNGEVFDAAAVKANIDTVLQNRP
CEEECCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHCCC
RHNWLELVNQMVSAEVVGPYKVRINLKKPYYPLLQELSLPRPFRFIAPSQFKNGGTADGI
CHHHHHHHHHHHHHEECCCEEEEEEECCCHHHHHHHCCCCCCEEECCCHHCCCCCCCCCE
VAPIGTGPWKLTETKLGEHDVFTRNDSYWGPKPAYEQITVKVIPDPNTRAIAFEAGEIDL
EEECCCCCCEEECCCCCCCCCEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCEEE
IYGTEGPISPDTFERFQKMGIYNTELSEPLETRVLALNTNHGATKDLAVRKAINHAVDKD
EECCCCCCCHHHHHHHHHCCCCCCHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCC
TMIATVLYGTQKRADTLFADNVPYANIGLKPYAFDPALAARLLDEAGWTAKASGDIREKD
CEEEEEEECCCHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCC
GQPLAIELCFIGTDAISKSMAEIVQADLRKVGIDVKLTGEEESSIYARQRDGRFDMIFNQ
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCCCEEEEEEC
TWGAPYDPHAFVSSMRVPSHADYQAQLGLPDKAKIDAEIGQVLVSTDETARQALYKDILT
CCCCCCCHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHEEECCCHHHHHHHHHHHHH
RLHEEAVYLPLTSVTAMAVAKPEVGKITFGAMSSEIPFEKLTPKAN
HHHHCEEEEEHHHHHHHHCCCCCCCEEEECCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MLTDRLRGLMLAFAFVSVAFVSGAHADPKLNFSWPVNVGPLNPHLYSPNQMFAQNMVYEP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCHHHHHHHHHHCC
LVHYNADGTVGPWLAESWEASQDGRSYTFKLREDVKFSNGEVFDAAAVKANIDTVLQNRP
CEEECCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHCCC
RHNWLELVNQMVSAEVVGPYKVRINLKKPYYPLLQELSLPRPFRFIAPSQFKNGGTADGI
CHHHHHHHHHHHHHEECCCEEEEEEECCCHHHHHHHCCCCCCEEECCCHHCCCCCCCCCE
VAPIGTGPWKLTETKLGEHDVFTRNDSYWGPKPAYEQITVKVIPDPNTRAIAFEAGEIDL
EEECCCCCCEEECCCCCCCCCEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCEEE
IYGTEGPISPDTFERFQKMGIYNTELSEPLETRVLALNTNHGATKDLAVRKAINHAVDKD
EECCCCCCCHHHHHHHHHCCCCCCHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCC
TMIATVLYGTQKRADTLFADNVPYANIGLKPYAFDPALAARLLDEAGWTAKASGDIREKD
CEEEEEEECCCHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCC
GQPLAIELCFIGTDAISKSMAEIVQADLRKVGIDVKLTGEEESSIYARQRDGRFDMIFNQ
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCCCEEEEEEC
TWGAPYDPHAFVSSMRVPSHADYQAQLGLPDKAKIDAEIGQVLVSTDETARQALYKDILT
CCCCCCCHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHEEECCCHHHHHHHHHHHHH
RLHEEAVYLPLTSVTAMAVAKPEVGKITFGAMSSEIPFEKLTPKAN
HHHHCEEEEEHHHHHHHHCCCCCCCEEEECCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]