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The map label for this gene is ispA

Identifier: 16273345

GI number: 16273345

Start: 1526433

End: 1527320

Strand: Direct

Name: ispA

Synonym: HI1438

Alternate gene names: 16273345

Gene position: 1526433-1527320 (Clockwise)

Preceding gene: 16273344

Following gene: 16273346

Centisome position: 83.41

GC content: 38.06

Gene sequence:

>888_bases
ATGGGTCACTTTTCTGAAGAATTACAACAGGTTCAAACTCGTATCAATCGCTTTTTAGAAGCTCAATTTGAAGGCATTGA
AAGTCATAATGCGCCTTTGCTTGAGGCGATGAAATACGCATTATTACTTGGCGGTAAGCGAGTTCGCCCTTTCTTAGTTT
ACGCAACAGGTCAAATGCTTGGCGCAGAGAAACAAACCTTAGATTACGCTGCTGCTGCCATTGAAGCCATTCACGCCTAT
TCCTTAATTCACGATGATTTACCTGCAATGGATGATGACAATTTACGCCGTGGACATCCTACTTGTCATATCCAATTTGA
TGAAGCTACAGCTATTCTTGCGGGCGATGCACTGCAAAGTTTCGCATTTGAAATATTAACCAAAACACCGAATATTTCTA
CTGAACAAAAACTGGCTTTAATTCAAATTTTAGCGCAAGGCGCTGGTGTGCAAGGAATGTGTTTAGGGCAAAGTTTAGAT
CTTATTTCTGAGCATAAACAGATTAGTTTAAGTGAATTAGAATTAATTCATCGTAACAAAACGGGTGCATTGCTAATTGC
CGCATTGAAATTAGGTTTCATTTGTTCTCCGCATTTTACCGACAAAAGGTTAGAACAATCCTTAACACAATATGCGGAAG
CCATTGGTTTAGCCTTTCAAGTTCAAGACGATATTTTAGATATTGAAGGCGATAGTGCAGAAATTGGCAAACAGGTGGGT
GCCGATCTTGATTTAGATAAAAGTACATATCCAAAATTACTTGGATTAAGTGGTGCAAAACAAAAAGCGCAAGATCTATA
TCAAAGTGCGTTGTCTGAATTAGAAAAAATTCCTTTTGATACAACCGTTCGTGCATTAGCTGAATTCATTATTACCAGAA
AAAGTTAA

Upstream 100 bases:

>100_bases
GTTTACAACAAGCAGAACAACGTATTCAGATCTTATTGCAAAAAACAGAAGACGCGCCACTGAATGATTATAAAGGGAAT
GATTATGAAGGGAATGCTTA

Downstream 100 bases:

>100_bases
ATACCCACAAAAGTGCGGTCAAAATTTATCACGTTTTTAAAATGACTAACAATATGAACAATTATCCTCTTTTATCTTTA
ATTAATTCTCCAGAAGATTT

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MGHFSEELQQVQTRINRFLEAQFEGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQMLGAEKQTLDYAAAAIEAIHAY
SLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQSFAFEILTKTPNISTEQKLALIQILAQGAGVQGMCLGQSLD
LISEHKQISLSELELIHRNKTGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTVRALAEFIITRKS

Sequences:

>Translated_295_residues
MGHFSEELQQVQTRINRFLEAQFEGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQMLGAEKQTLDYAAAAIEAIHAY
SLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQSFAFEILTKTPNISTEQKLALIQILAQGAGVQGMCLGQSLD
LISEHKQISLSELELIHRNKTGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTVRALAEFIITRKS
>Mature_294_residues
GHFSEELQQVQTRINRFLEAQFEGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQMLGAEKQTLDYAAAAIEAIHAYS
LIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQSFAFEILTKTPNISTEQKLALIQILAQGAGVQGMCLGQSLDL
ISEHKQISLSELELIHRNKTGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVGA
DLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTVRALAEFIITRKS

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Homo sapiens, GI50659086, Length=231, Percent_Identity=29.4372294372294, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786623, Length=297, Percent_Identity=53.1986531986532, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1789578, Length=267, Percent_Identity=29.5880149812734, Blast_Score=101, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17505681, Length=186, Percent_Identity=29.5698924731183, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24651612, Length=219, Percent_Identity=29.6803652968037, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPA_HAEIN (P45204)

Other databases:

- EMBL:   L42023
- PIR:   C64123
- RefSeq:   NP_439590.1
- ProteinModelPortal:   P45204
- SMR:   P45204
- GeneID:   950833
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1438
- NMPDR:   fig|71421.1.peg.1372
- TIGR:   HI_1438
- HOGENOM:   HBG732667
- OMA:   KPTNHKI
- ProtClustDB:   CLSK870150
- BioCyc:   HINF71421:HI_1438-MONOMER
- BRENDA:   2.5.1.10
- GO:   GO:0005737
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10
- PANTHER:   PTHR12001

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.10

Molecular weight: Translated: 32407; Mature: 32276

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 46-46 BINDING 49-49 BINDING 78-78 BINDING 96-96 BINDING 97-97 BINDING 180-180 BINDING 181-181 BINDING 220-220 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHFSEELQQVQTRINRFLEAQFEGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQML
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHH
GAEKQTLDYAAAAIEAIHAYSLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
FAFEILTKTPNISTEQKLALIQILAQGAGVQGMCLGQSLDLISEHKQISLSELELIHRNK
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHCCC
TGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCEEECCCCHHHHHHHHC
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTVRALAEFIITRKS
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GHFSEELQQVQTRINRFLEAQFEGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQML
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHH
GAEKQTLDYAAAAIEAIHAYSLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
FAFEILTKTPNISTEQKLALIQILAQGAGVQGMCLGQSLDLISEHKQISLSELELIHRNK
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHCCC
TGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCEEECCCCHHHHHHHHC
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTVRALAEFIITRKS
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800