Click here to switch to the map view.

The map label for this gene is purN

Identifier: 16273333

GI number: 16273333

Start: 1516529

End: 1517167

Strand: Reverse

Name: purN

Synonym: HI1428

Alternate gene names: 16273333

Gene position: 1517167-1516529 (Counterclockwise)

Preceding gene: 16273334

Following gene: 16273329

Centisome position: 82.9

GC content: 40.06

Gene sequence:

>639_bases
ATGAAAAAAATTGCCGTCCTTATTTCTGGTCAAGGAACAAATTTACAAACTATTATTGATGCCTGCCATTCAGGCGATAT
TCCCGCCAAAATTGCTTGTGTGATAAGTAACAAAGCAGATGCTTACGGCTTAGTTCGAGCCAAACAAGCACAGATTCCGC
AAGCGGTCTTTCTTCGCAAAAATTTTTCCAATAACCTTGAAATGGACGACGCAATTGGCGATTATTTGCAAAGCCTAGCT
GTAGATTTGATCGTCCTTGCTGGTTATATGAAAATTCTTACCCCAAAATTTACCCAACGTTTTGCAGGGAAAATTTTAAA
TATCCATCCTTCTCTTCTTCCTAAATACGCAGGCTTAAACACCTACCAACGTGCGATTGAAGCAGGCGACAATGAACACG
GCACAACCGTTCATTTTGTCAATGAAGAAGTAGATGGTGGTGCGATTGTCTTACAAGCAAAAGTGCCAATCTTCCCTGAA
GATAGCATCGAAGAAGTGGAGGCTAGAACCCGTGAGCAAGAATATCAAATTTATCCGCTTGTGATTAAATGGTTTACCGA
AGGGCGGTTAAGACTTAAAGATAATTTGGCATATTTAGATGGGAAAGCTCTGCCAAAATCTGGATATGCAAATGAATAA

Upstream 100 bases:

>100_bases
AATTGAACATCTTGGCGAAAGCGAGGCACAGGTTGAGATTCAATAATAAAATCTTAAATTTAAATTCAGCCCTTTTGGGC
TGATTTCTATAAGGAAGAAA

Downstream 100 bases:

>100_bases
CTTAGAATTTAGACAGACTATTTCTGTATTTTTTATTTAATTAGGATGTAAAAAGTGCGGTGAAATTTTACCGCACTTTT
AAAATTAGTGATGATGGTGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLRKNFSNNLEMDDAIGDYLQSLA
VDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPE
DSIEEVEARTREQEYQIYPLVIKWFTEGRLRLKDNLAYLDGKALPKSGYANE

Sequences:

>Translated_212_residues
MKKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLRKNFSNNLEMDDAIGDYLQSLA
VDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPE
DSIEEVEARTREQEYQIYPLVIKWFTEGRLRLKDNLAYLDGKALPKSGYANE
>Mature_212_residues
MKKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLRKNFSNNLEMDDAIGDYLQSLA
VDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPE
DSIEEVEARTREQEYQIYPLVIKWFTEGRLRLKDNLAYLDGKALPKSGYANE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=42.4870466321244, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=42.4870466321244, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=42.4870466321244, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=207, Percent_Identity=52.6570048309179, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1787483, Length=176, Percent_Identity=30.6818181818182, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=37.2881355932203, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=33.8383838383838, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=44.973544973545, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR3_HAEIN (P43846)

Other databases:

- EMBL:   L42023
- PIR:   F64122
- RefSeq:   NP_439577.1
- ProteinModelPortal:   P43846
- SMR:   P43846
- GeneID:   950331
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1428
- NMPDR:   fig|71421.1.peg.1360
- TIGR:   HI_1428
- HOGENOM:   HBG649382
- OMA:   AGDSEHG
- ProtClustDB:   PRK05647
- BioCyc:   HINF71421:HI_1428-MONOMER
- BRENDA:   2.1.2.2
- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607
- Gene3D:   G3DSA:3.40.50.170
- TIGRFAMs:   TIGR00639

Pfam domain/function: PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: =2.1.2.2

Molecular weight: Translated: 23463; Mature: 23463

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00373 GART

Important sites: ACT_SITE 109-109 BINDING 107-107 BINDING 145-145 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLRK
CCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHEEHHHHCCCHHHHHHH
NFSNNLEMDDAIGDYLQSLAVDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCHHHHHHHCCCH
TYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPEDSIEEVEARTREQEYQIYPL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEE
VIKWFTEGRLRLKDNLAYLDGKALPKSGYANE
EEEEECCCCEEEECCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLRK
CCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHEEHHHHCCCHHHHHHH
NFSNNLEMDDAIGDYLQSLAVDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCHHHHHHHCCCH
TYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPEDSIEEVEARTREQEYQIYPL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEE
VIKWFTEGRLRLKDNLAYLDGKALPKSGYANE
EEEEECCCCEEEECCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800