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The map label for this gene is cafA

Identifier: 16273263

GI number: 16273263

Start: 1430514

End: 1431989

Strand: Direct

Name: cafA

Synonym: HI1353

Alternate gene names: 16273263

Gene position: 1430514-1431989 (Clockwise)

Preceding gene: 16273260

Following gene: 16273264

Centisome position: 78.16

GC content: 39.5

Gene sequence:

>1476_bases
ATGGATGCTGTCGAGTTATTGATGAACGTAACGCCTAACGAAACACGCATTGCGCTAGTAGAAACAGGAATGTTGCGCGA
AGTGCATATTGAACGCCAAGCTAAACGAGGAATTGTCGGAAACATTTATAAAGGTCGTGTCACTCGAGTGTTACCAGGAA
TGCAGTCGGCTTTTGTTGATATTGGTTTGGAAAAAGCGGCTTTTTTACACGCTGCGGACATTGTATCCCATACAGAATGT
GTAGATGAAAATGAACAAAAGCAATTTAAAGTTAAGAGCATTTCAGAATTAGTACGAGAAGGGCAGGATATTGTTGTACA
AGTGGTGAAAGAGCCGCTAGGTACAAAAGGTGCACGTTTAACAACGGATATCACATTGCCATCGCGTCATCTTGTTTTTA
TGCCTGAAAATAGCCACGTAGGCGTTTCACAACGCATTGAAAGCGAAGAAGAACGCGCCCGTTTAAAAGCATTAGTTGAA
CCTTTTTGTGATGAACTCGGCGGTTTTATTATTCGTACCGCCACAGAAGGGGCAAGCGAAGAAGAATTACGTCAAGACGC
TGAATTTTTAAAACGCTTATGGCGTAAAGTTTTAGAACGTAAATCTAAGTACCCAACCAAATCAAAAATTTACGGCGAGC
CAGCACTTCCACAACGTATTTTGCGCGATTTTATCGGCACAAACTTAGAAAAAATTCGTATCGACTCTAAACTTTGCTTT
GGCGAAGTAAAAGAGTTTACCGATGAATTTATGCCTGAATTAAGCGATAAACTTGTTCTTTATTCTGGCAATCAACCTAT
CTTTGATGTGTACGGGGTTGAAAATGCGATCCAAACAGCCTTAGATAAACGTGTTAATCTCAAATCGGGCGGCTATTTAA
TTATCGAGCAAACAGAAGCCATGACCACCATTGATATTAATACAGGCGCATTTGTGGGGCATCGTAATCTAGAAGAAACC
ATCTTCAATACCAATATTGAAGCAACTAAAGCCATTGCGCACGAACTCCAACTACGTAATCTTGGTGGCATAATCATTAT
TGATTTTATTGATATGCAAACAGATGAACATCGCAATCGAGTATTGCAATCTTTATGTGATGCGCTTTCTAAAGATCGTA
TGAAAACTAATGTGAATGGCTTTACGCAATTAGGCCTTGTAGAAATGACTCGTAAAAGAACCCGCGAAAGCCTTGAACAC
GTGCTATGCGATGAATGTCCAACTTGTCATGGACGTGGTCGGGTGAAAACTGTTGAGACAGTTTGCTACGAAATTATGCG
TGAAATTATTCGGGTGTATCATTTATTCAGTAGTGAACAATTTGTTGTTTATGCCTCGCCAGCAGTTTCAGAATATCTTA
TTAATGAAGAATCTCACGGTTTACTGCCAGAAGTGGAAATGTTTATTGGAAAACGAGTAAAAGTAAAAACAGAACAGTTT
TATAACCAAGAACAGTTTGATGTGGTTGTAATGTAA

Upstream 100 bases:

>100_bases
TGGGTTAAAAATTTCAGAAAGGGTGCATTTTACCTTATTTCGGGCTATTATTCTATTTAGACTAAATATTTGATGAAATT
TAAGAAATTAGGAATATAAA

Downstream 100 bases:

>100_bases
ACTCTATTTCAATAAAGTGCGGTAAAAATTTTTGAGACTTTCACCGCACTTTTTATTTATAAAAACAATATTTCCTTTAT
ATTTTATCTATATGACGTAA

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MDAVELLMNVTPNETRIALVETGMLREVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHAADIVSHTEC
VDENEQKQFKVKSISELVREGQDIVVQVVKEPLGTKGARLTTDITLPSRHLVFMPENSHVGVSQRIESEEERARLKALVE
PFCDELGGFIIRTATEGASEEELRQDAEFLKRLWRKVLERKSKYPTKSKIYGEPALPQRILRDFIGTNLEKIRIDSKLCF
GEVKEFTDEFMPELSDKLVLYSGNQPIFDVYGVENAIQTALDKRVNLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLEET
IFNTNIEATKAIAHELQLRNLGGIIIIDFIDMQTDEHRNRVLQSLCDALSKDRMKTNVNGFTQLGLVEMTRKRTRESLEH
VLCDECPTCHGRGRVKTVETVCYEIMREIIRVYHLFSSEQFVVYASPAVSEYLINEESHGLLPEVEMFIGKRVKVKTEQF
YNQEQFDVVVM

Sequences:

>Translated_491_residues
MDAVELLMNVTPNETRIALVETGMLREVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHAADIVSHTEC
VDENEQKQFKVKSISELVREGQDIVVQVVKEPLGTKGARLTTDITLPSRHLVFMPENSHVGVSQRIESEEERARLKALVE
PFCDELGGFIIRTATEGASEEELRQDAEFLKRLWRKVLERKSKYPTKSKIYGEPALPQRILRDFIGTNLEKIRIDSKLCF
GEVKEFTDEFMPELSDKLVLYSGNQPIFDVYGVENAIQTALDKRVNLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLEET
IFNTNIEATKAIAHELQLRNLGGIIIIDFIDMQTDEHRNRVLQSLCDALSKDRMKTNVNGFTQLGLVEMTRKRTRESLEH
VLCDECPTCHGRGRVKTVETVCYEIMREIIRVYHLFSSEQFVVYASPAVSEYLINEESHGLLPEVEMFIGKRVKVKTEQF
YNQEQFDVVVM
>Mature_491_residues
MDAVELLMNVTPNETRIALVETGMLREVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLEKAAFLHAADIVSHTEC
VDENEQKQFKVKSISELVREGQDIVVQVVKEPLGTKGARLTTDITLPSRHLVFMPENSHVGVSQRIESEEERARLKALVE
PFCDELGGFIIRTATEGASEEELRQDAEFLKRLWRKVLERKSKYPTKSKIYGEPALPQRILRDFIGTNLEKIRIDSKLCF
GEVKEFTDEFMPELSDKLVLYSGNQPIFDVYGVENAIQTALDKRVNLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLEET
IFNTNIEATKAIAHELQLRNLGGIIIIDFIDMQTDEHRNRVLQSLCDALSKDRMKTNVNGFTQLGLVEMTRKRTRESLEH
VLCDECPTCHGRGRVKTVETVCYEIMREIIRVYHLFSSEQFVVYASPAVSEYLINEESHGLLPEVEMFIGKRVKVKTEQF
YNQEQFDVVVM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI87082235, Length=487, Percent_Identity=71.2525667351129, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=455, Percent_Identity=36.043956043956, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNG_HAEIN (P45175)

Other databases:

- EMBL:   L42023
- PIR:   F64118
- RefSeq:   NP_439504.1
- ProteinModelPortal:   P45175
- SMR:   P45175
- GeneID:   950270
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1353
- NMPDR:   fig|71421.1.peg.1289
- TIGR:   HI_1353
- HOGENOM:   HBG756312
- OMA:   HQRQVLR
- ProtClustDB:   PRK11712
- BioCyc:   HINF71421:HI_1353-MONOMER
- GO:   GO:0005737
- GO:   GO:0005856
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316
- TIGRFAMs:   TIGR00757

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55929; Mature: 55929

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVELLMNVTPNETRIALVETGMLREVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVD
CCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHCHHHHHCCCHHHHHHH
IGLEKAAFLHAADIVSHTECVDENEQKQFKVKSISELVREGQDIVVQVVKEPLGTKGARL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEE
TTDITLPSRHLVFMPENSHVGVSQRIESEEERARLKALVEPFCDELGGFIIRTATEGASE
EEEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
EELRQDAEFLKRLWRKVLERKSKYPTKSKIYGEPALPQRILRDFIGTNLEKIRIDSKLCF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHH
GEVKEFTDEFMPELSDKLVLYSGNQPIFDVYGVENAIQTALDKRVNLKSGGYLIIEQTEA
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEEECCC
MTTIDINTGAFVGHRNLEETIFNTNIEATKAIAHELQLRNLGGIIIIDFIDMQTDEHRNR
EEEEECCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
VLQSLCDALSKDRMKTNVNGFTQLGLVEMTRKRTRESLEHVLCDECPTCHGRGRVKTVET
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
VCYEIMREIIRVYHLFSSEQFVVYASPAVSEYLINEESHGLLPEVEMFIGKRVKVKTEQF
HHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEHHHH
YNQEQFDVVVM
CCCCCEEEEEC
>Mature Secondary Structure
MDAVELLMNVTPNETRIALVETGMLREVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVD
CCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHCHHHHHCCCHHHHHHH
IGLEKAAFLHAADIVSHTECVDENEQKQFKVKSISELVREGQDIVVQVVKEPLGTKGARL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEE
TTDITLPSRHLVFMPENSHVGVSQRIESEEERARLKALVEPFCDELGGFIIRTATEGASE
EEEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
EELRQDAEFLKRLWRKVLERKSKYPTKSKIYGEPALPQRILRDFIGTNLEKIRIDSKLCF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHH
GEVKEFTDEFMPELSDKLVLYSGNQPIFDVYGVENAIQTALDKRVNLKSGGYLIIEQTEA
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEEECCC
MTTIDINTGAFVGHRNLEETIFNTNIEATKAIAHELQLRNLGGIIIIDFIDMQTDEHRNR
EEEEECCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
VLQSLCDALSKDRMKTNVNGFTQLGLVEMTRKRTRESLEHVLCDECPTCHGRGRVKTVET
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
VCYEIMREIIRVYHLFSSEQFVVYASPAVSEYLINEESHGLLPEVEMFIGKRVKVKTEQF
HHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEHHHH
YNQEQFDVVVM
CCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800