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The map label for this gene is glnS [H]

Identifier: 16273264

GI number: 16273264

Start: 1432409

End: 1434082

Strand: Direct

Name: glnS [H]

Synonym: HI1354

Alternate gene names: 16273264

Gene position: 1432409-1434082 (Clockwise)

Preceding gene: 16273263

Following gene: 16273265

Centisome position: 78.27

GC content: 39.43

Gene sequence:

>1674_bases
ATGATGAGCCATACAGAGACATCTTTGGGTGCAGAAAATACCCGCACTCATAATTTTATTACCCAAATTATTGATGAAGA
TTTAGCTTCGGGTAAACATAAAAGCGTTCATACCCGTTTTCCGCCAGAGCCGAATGGTTATTTGCATATTGGTCACGCAA
AGTCTATTTGCTTGAATTTTGGCTTGGCAAAAGAATACCAAGGTTTATGTAACCTACGTTTTGACGATACCAATCCAGTA
AAAGAAGACGTGGAATATGTAGATTCTATCAAAGCGGATGTGGAATGGTTAGGTTTTAAATGGGAGGGCGAACCGCGTTA
TGCATCTGATTACTTCGATGCGCTTTATGGCTATGCCGTTGAATTAATTAAAAAAGGTTTGGCTTATGTTGATGAGCTTT
CTCCAGATGAAATGCGTGAATATCGTGGTACCTTAACCGAACCTGGTAAAAACAGCCCATATCGTGACCGCACTATTGAA
GAAAACTTAGCGTTATTTGAAAAAATGAAAAATGGCGAATTTGCCGAGGGTAAAGCAAGTTTACGTGCAAAAATTGATAT
GGCTTCGCCATTTATGGTCATGCGTGAACCAGTAATTTATCGTATTAAATTCTCTAGCCATCACCAAACAGGTGACAAAT
GGTGTATTTACCCAATGTACGATTTCACGCACTGTATCTCTGATGCGATTGAGCGTATTACACACTCAATCTGTACATTA
GAATTCCAAGATAACCGTCGTTTATATGACTGGGTGTTGGAAAATATCAGTATTGAACGTCCATTACCGCATCAATATGA
ATTCTCACGTTTGAATTTAGAAGGCACTTTAACCTCTAAACGTAAATTATTGAAATTGGTAAATGATGAAATCGTGGATG
GTTGGAACGATCCACGTATGCCAACCATTTCAGGTTTACGCCGTCGTGGTTATACACCAGCTTCGTTACGTGAATTTTGT
CGTCGTATCGGTGTGACTAAACAAGATAACGTCGTAGAGTATTCAGCACTTGAAGCCTGCATTCGTGAGGATTTAAATGA
AAATGCACCACGCGCAATGGCAGTGATTGATCCTGTTCGTGTGGTAATTGAAAATTTTGAAAGCGAAGCGGTTTTAACTG
CCCCAAATCATCCAAATCGTCCTGAATTAGGGGAACGTCAATTGCCGTTCACGAAAGAACTTTATATTGATCGAGCTGAC
TTCCGTGAAGAAGCGAACAAACAATATAAACGCTTAGTATTAGGCAAAGAAGTGCGTTTGCGTAATGCTTATGTGATTAA
AGCTGAACGTGTAGAAAAAGATGCAAATGGTGAAATCACCACGATCTTCTGTACTTACGATCCTGAAACTTTAGGTAAAA
ATCCAGCAGATGGACGTAAAGTGAAAGGTGTCATTCATTGGGTTTCTGCGGTTAATAATCATCCAGCTGAATTCCGTTTG
TATGATCGCTTATTTACTGTGCCAAATCCAGGGGCTGAAGATGATATCGAAAGCGTGTTAAACCCAAATTCTTTAGTGAT
AAAACAAGGTTTTGTAGAGCAAAGCCTCGCAAATGCAGAAGCAGAGAAAGGCTACCAATTTGAACGTGAGGGTTATTTCT
GTGCAGATAGTAAAGATAGCCGACCTGAACATTTGGTATTTAACTTAACAGTAAGCTTAAAAGAAGGCTTCTAA

Upstream 100 bases:

>100_bases
TTAAATCTAGTATTTCTATGGCAAGAACAGGAGATGAACCTTTATTTTCCCTTAGATTTGTATAGAATAGCCGAATATTT
TTTTATTTTAACGAGAGAAA

Downstream 100 bases:

>100_bases
TTTTAATCAACCAAGTGCGGTCAAAACAAACGTGATTTTGACCGCACTTTTTATTTGTATCAACTATGCAATCCAATATG
CAACTTGAACCCAATTTCTG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MMSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPV
KEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAVELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIE
ENLALFEKMKNGEFAEGKASLRAKIDMASPFMVMREPVIYRIKFSSHHQTGDKWCIYPMYDFTHCISDAIERITHSICTL
EFQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMPTISGLRRRGYTPASLREFC
RRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRAD
FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDANGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRL
YDRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF

Sequences:

>Translated_557_residues
MMSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPV
KEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAVELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIE
ENLALFEKMKNGEFAEGKASLRAKIDMASPFMVMREPVIYRIKFSSHHQTGDKWCIYPMYDFTHCISDAIERITHSICTL
EFQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMPTISGLRRRGYTPASLREFC
RRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRAD
FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDANGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRL
YDRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF
>Mature_557_residues
MMSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPV
KEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAVELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIE
ENLALFEKMKNGEFAEGKASLRAKIDMASPFMVMREPVIYRIKFSSHHQTGDKWCIYPMYDFTHCISDAIERITHSICTL
EFQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRMPTISGLRRRGYTPASLREFC
RRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRAD
FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDANGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRL
YDRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=47.3584905660377, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI62241042, Length=517, Percent_Identity=33.2688588007737, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1786895, Length=546, Percent_Identity=76.5567765567766, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=303, Percent_Identity=22.4422442244224, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=562, Percent_Identity=40.3914590747331, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17511007, Length=521, Percent_Identity=31.8618042226488, Blast_Score=244, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6324742, Length=563, Percent_Identity=41.3854351687389, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321192, Length=526, Percent_Identity=35.5513307984791, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17864488, Length=542, Percent_Identity=42.9889298892989, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24649466, Length=519, Percent_Identity=34.2967244701349, Blast_Score=268, Evalue=9e-72,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 64079; Mature: 64079

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHC
GLAKEYQGLCNLRFDDTNPVKEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAV
CCHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
ELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEENLALFEKMKNGEFAEGKAS
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
LRAKIDMASPFMVMREPVIYRIKFSSHHQTGDKWCIYPMYDFTHCISDAIERITHSICTL
CEEEEECCCCHHHHCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEE
EFQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRM
EECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCC
PTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVR
CHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
VVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRADFREEANKQYKRLVLGKEVRL
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEE
RNAYVIKAERVEKDANGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRL
CCEEEEEHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
YDRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDS
EEHEEECCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHCCCEECCCCEEECCCCCC
RPEHLVFNLTVSLKEGF
CCCEEEEEEEEEEECCC
>Mature Secondary Structure
MMSHTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHC
GLAKEYQGLCNLRFDDTNPVKEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYGYAV
CCHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
ELIKKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRTIEENLALFEKMKNGEFAEGKAS
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
LRAKIDMASPFMVMREPVIYRIKFSSHHQTGDKWCIYPMYDFTHCISDAIERITHSICTL
CEEEEECCCCHHHHCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEE
EFQDNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNDEIVDGWNDPRM
EECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCC
PTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVR
CHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
VVIENFESEAVLTAPNHPNRPELGERQLPFTKELYIDRADFREEANKQYKRLVLGKEVRL
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEE
RNAYVIKAERVEKDANGEITTIFCTYDPETLGKNPADGRKVKGVIHWVSAVNNHPAEFRL
CCEEEEEHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
YDRLFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLANAEAEKGYQFEREGYFCADSKDS
EEHEEECCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHCCCEECCCCEEECCCCCC
RPEHLVFNLTVSLKEGF
CCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA