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The map label for this gene is rumA [H]

Identifier: 16272285

GI number: 16272285

Start: 359839

End: 361155

Strand: Direct

Name: rumA [H]

Synonym: HI0333

Alternate gene names: 16272285

Gene position: 359839-361155 (Clockwise)

Preceding gene: 16272284

Following gene: 16272286

Centisome position: 19.66

GC content: 37.89

Gene sequence:

>1317_bases
ATGGTTCTTCTTTATACCCCAAAGCAAAAAACAAAAAATGTTCAAACCATTACGGCTGATATTCTTGATTTAGATTATCA
AGGTTTAGGCGTGGCGAAAATTAATGGTAAAACTTGGTTTATTGAAAATGCGCTTCCGCACGAAAAAGTGGAATGTCGTA
TTTTGGAAGATAAACGCCAATATGGACACGCCATTGTAAAAAAATGGCGTGTAAAAAGCCCTGAACGTTTAGAACCTAAA
TGTGCTCATTTTATGCGTTGTGGTGGTTGCCAAGGACAGCATATTCCTATTGAAATGCAGAGAAAAGCGAAAGAATCTGC
ATTGTTTAAAAGATTAAGTAAATTACAGTCAGAACCGATTTCATTTCAACCTATGATTTGTGGCGATGCTTGGGCGTATC
GTCGTCGTGTGCGTTTGAGTTTATGGTTTAACCCAAGCACAAAACAAATCGAAATGGGCTTTCGTCAGAAAAATACCAAC
GATTTAATACCCGTTCAATCTTGCGAAGTAGCAGAACCTGCAATTAATTATTTACTGCCTAAATTAACCGCACTTTTAGA
AAAATTTTCAGCACCGAAACAGCTTGGGCATATTGAATTGGTGGCTGCCGATAATGGTGTCGCAATGTTGTTACGTTACA
CCAAAAACCTTGCGGAAATTGACCGCACTTTATTGCTTAAATTTGCTGAACAAGAAAAGTTAATGCTGTTTTTACAAAGC
GACAAAGGCATTGAACAAATCTATGGCGATGCACCTTATTATCAATTTTCTGATGGCATAAAATTACATTTTGATATTCG
TGATTTTATTCAAGTAAATAGTGCATTAAATGAACCTATGGTCAATACAGCGTTGGATTGGCTTGAATTGAGCCAACAGG
ATTGTGTGTTAGATTTATTTTGCGGCATGGGAAATTTTACGCTTCCTCTCGCCAAACGCGTGAAAAGTGCGGTAGGAATT
GAAGGTGTTTTTGAAATGGTACAAAAAGCAGCGCAAAATGCCGAGAGAAACCAAATCAAAAATATTGAATTTTTCCAAGC
GGATCTTGACCAATCCTTTGTTGAACAACCTTGGGCTAATCAGTCGTTCAATAAAATTTTGCTTGATCCTCCACGCAGTG
GCGCAGCTTTCGCGTTAAATGCGTTGTGCGAGTTGAAAGCAGAGAAAATTTTATATGTGTCTTGTAATCCCGCAACATTA
GTGCGCGATGCGGAAATTTTATGTAATTTTGGCTACAAGATTGAAAAAAGTGCGGTGATAGATATGTTCCCTCATACGGG
GCATTTGGAATCGATAACCTTATTCACTACAAAATAA

Upstream 100 bases:

>100_bases
CGGCAAAACGTTTTACTCGTATTGCACTGAAACCTTATCTTGGCGATAAACCGCTAAAAAGCCGAGAGTTATTCACTCAA
AATATTTTACTTTTAAAATA

Downstream 100 bases:

>100_bases
CTCGGTTATTCGGTACAATATCCACCCAAAATAAAAGCGACAGGAAGTTGCTTGCTCTTCATCAACAAGGGGGTATTATG
GTTGCTGTTCGTGGTTCTCA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVLLYTPKQKTKNVQTITADILDLDYQGLGVAKINGKTWFIENALPHEKVECRILEDKRQYGHAIVKKWRVKSPERLEPK
CAHFMRCGGCQGQHIPIEMQRKAKESALFKRLSKLQSEPISFQPMICGDAWAYRRRVRLSLWFNPSTKQIEMGFRQKNTN
DLIPVQSCEVAEPAINYLLPKLTALLEKFSAPKQLGHIELVAADNGVAMLLRYTKNLAEIDRTLLLKFAEQEKLMLFLQS
DKGIEQIYGDAPYYQFSDGIKLHFDIRDFIQVNSALNEPMVNTALDWLELSQQDCVLDLFCGMGNFTLPLAKRVKSAVGI
EGVFEMVQKAAQNAERNQIKNIEFFQADLDQSFVEQPWANQSFNKILLDPPRSGAAFALNALCELKAEKILYVSCNPATL
VRDAEILCNFGYKIEKSAVIDMFPHTGHLESITLFTTK

Sequences:

>Translated_438_residues
MVLLYTPKQKTKNVQTITADILDLDYQGLGVAKINGKTWFIENALPHEKVECRILEDKRQYGHAIVKKWRVKSPERLEPK
CAHFMRCGGCQGQHIPIEMQRKAKESALFKRLSKLQSEPISFQPMICGDAWAYRRRVRLSLWFNPSTKQIEMGFRQKNTN
DLIPVQSCEVAEPAINYLLPKLTALLEKFSAPKQLGHIELVAADNGVAMLLRYTKNLAEIDRTLLLKFAEQEKLMLFLQS
DKGIEQIYGDAPYYQFSDGIKLHFDIRDFIQVNSALNEPMVNTALDWLELSQQDCVLDLFCGMGNFTLPLAKRVKSAVGI
EGVFEMVQKAAQNAERNQIKNIEFFQADLDQSFVEQPWANQSFNKILLDPPRSGAAFALNALCELKAEKILYVSCNPATL
VRDAEILCNFGYKIEKSAVIDMFPHTGHLESITLFTTK
>Mature_438_residues
MVLLYTPKQKTKNVQTITADILDLDYQGLGVAKINGKTWFIENALPHEKVECRILEDKRQYGHAIVKKWRVKSPERLEPK
CAHFMRCGGCQGQHIPIEMQRKAKESALFKRLSKLQSEPISFQPMICGDAWAYRRRVRLSLWFNPSTKQIEMGFRQKNTN
DLIPVQSCEVAEPAINYLLPKLTALLEKFSAPKQLGHIELVAADNGVAMLLRYTKNLAEIDRTLLLKFAEQEKLMLFLQS
DKGIEQIYGDAPYYQFSDGIKLHFDIRDFIQVNSALNEPMVNTALDWLELSQQDCVLDLFCGMGNFTLPLAKRVKSAVGI
EGVFEMVQKAAQNAERNQIKNIEFFQADLDQSFVEQPWANQSFNKILLDPPRSGAAFALNALCELKAEKILYVSCNPATL
VRDAEILCNFGYKIEKSAVIDMFPHTGHLESITLFTTK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=259, Percent_Identity=29.3436293436293, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI51173878, Length=259, Percent_Identity=29.3436293436293, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1789148, Length=437, Percent_Identity=46.6819221967963, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1787083, Length=375, Percent_Identity=25.6, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24666579, Length=210, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49870; Mature: 49870

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLYTPKQKTKNVQTITADILDLDYQGLGVAKINGKTWFIENALPHEKVECRILEDKRQ
CEEEECCCHHHCCHHEEEEHHHCCCCCCCEEEEECCEEEEEECCCCCCCEEEEEEHHHHH
YGHAIVKKWRVKSPERLEPKCAHFMRCGGCQGQHIPIEMQRKAKESALFKRLSKLQSEPI
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
SFQPMICGDAWAYRRRVRLSLWFNPSTKQIEMGFRQKNTNDLIPVQSCEVAEPAINYLLP
CCCEEECCCHHHHHEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLTALLEKFSAPKQLGHIELVAADNGVAMLLRYTKNLAEIDRTLLLKFAEQEKLMLFLQS
HHHHHHHHHCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DKGIEQIYGDAPYYQFSDGIKLHFDIRDFIQVNSALNEPMVNTALDWLELSQQDCVLDLF
CCCHHHHCCCCCCEEECCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHH
CGMGNFTLPLAKRVKSAVGIEGVFEMVQKAAQNAERNQIKNIEFFQADLDQSFVEQPWAN
HCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCC
QSFNKILLDPPRSGAAFALNALCELKAEKILYVSCNPATLVRDAEILCNFGYKIEKSAVI
CCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHCHHHHCCCCCEECCCEEE
DMFPHTGHLESITLFTTK
EECCCCCCCCEEEEEEEC
>Mature Secondary Structure
MVLLYTPKQKTKNVQTITADILDLDYQGLGVAKINGKTWFIENALPHEKVECRILEDKRQ
CEEEECCCHHHCCHHEEEEHHHCCCCCCCEEEEECCEEEEEECCCCCCCEEEEEEHHHHH
YGHAIVKKWRVKSPERLEPKCAHFMRCGGCQGQHIPIEMQRKAKESALFKRLSKLQSEPI
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
SFQPMICGDAWAYRRRVRLSLWFNPSTKQIEMGFRQKNTNDLIPVQSCEVAEPAINYLLP
CCCEEECCCHHHHHEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLTALLEKFSAPKQLGHIELVAADNGVAMLLRYTKNLAEIDRTLLLKFAEQEKLMLFLQS
HHHHHHHHHCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DKGIEQIYGDAPYYQFSDGIKLHFDIRDFIQVNSALNEPMVNTALDWLELSQQDCVLDLF
CCCHHHHCCCCCCEEECCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHH
CGMGNFTLPLAKRVKSAVGIEGVFEMVQKAAQNAERNQIKNIEFFQADLDQSFVEQPWAN
HCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCC
QSFNKILLDPPRSGAAFALNALCELKAEKILYVSCNPATLVRDAEILCNFGYKIEKSAVI
CCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHCHHHHCCCCCEECCCEEE
DMFPHTGHLESITLFTTK
EECCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA