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The map label for this gene is hcaT

Identifier: 16272263

GI number: 16272263

Start: 341452

End: 342618

Strand: Direct

Name: hcaT

Synonym: HI0308

Alternate gene names: 16272263

Gene position: 341452-342618 (Clockwise)

Preceding gene: 16272262

Following gene: 16272264

Centisome position: 18.66

GC content: 40.96

Gene sequence:

>1167_bases
ATGCAAGTCCGTCCTTTTTATTGGCTCGCACTGAGCTTTCTAGGTTACTACTGCGCTTATGGCGTGTTTTTGCCGTTCTT
CCCCGCATGGTTGAAATCGCAACAATATGGCGAAGAAATGATTGGTTTGGTGATCGGTAGTGCTTATATTTTCCGATTTG
CGGGCGGATTATTTTTTTCATCGCTGATTAAAAAAGCCAATCATATTATTATTGCCTTACGCCTATTGGCGTTAGCATCG
GCAATTATTATGGCGGCTATGAGTTTAGTTGCGCATAATTTTTGGCTGTTATTTATTGCTATCGGTTTGTATGCTTCTGT
GAATTCAGCAGGAATGCCGATTGGGGATTCGTTGGCATCCACTTGGCAACGCCAAATCGGCTTGGATTACGGCAAAGTAC
GTTTAATTGGCTCTGCTGCTTTTATCCTTGGCGTAGTGGTGTTCGGCGGCATGATTGGCTGGGTTGGCGAGCAAAATATC
GTGTGGATTTTAACCGCACTTTTATCTTTCTATACCATCATTCAGCTTTTAAAACCCACCATTCCCCCAAAAGATGAAAT
ACCTGAAGATAGCGTTCAAAATGATGTTGGTTTTATTGCTTTACTGAAAAATCCGATGACATTGCGAGTTATGATAGCAG
TAGGGCTGATTCAAGGCTCTCATGCGGCTTATTATGTTTATAGCACGATTTATTGGACAAGTATTGGGATCTCCGTTTCT
CAAACTAGCTTACTTTGGGGCATTGGCGTATTAGCGGAAATTGTATTATTTTTCTTCTCTCGTCGCTTATTCCAAAATAT
TTCTATCAGTGTGCTGCTTTATATTTCTGCGCTTGCGTGCGTTGGACGCTGGGCTGTAATAGGATATATTGAAGATTTCT
GGTTGATATTCTTATTGCAACTTATGCACAGTTTAACCTATGCCGTTTGCCATTATGCGATTGTTCGCTATATCACCACG
CAACCGCAAAGCCATATTGCGAAGTTACAAGGTTTGTATAATGGTTTATCCAATGGTGTGCTCATTGCTATTTTCACTGC
GATTGCTGGCATGATTTATCCAACGTCGCCAGCGATGACATTTGTATTTATGAGTATCATTGCAGCTGCTGCATTTTTTG
TGATTCCACGTAAGTTAAATGCCTTTTTGATTGTTCAGCATAAATAG

Upstream 100 bases:

>100_bases
CAGCGTTAGCAGTATTTGACGCACAACATTTTAATCAAACAATAGAGCAAGCTATGCAGGCTTGTTTGTCTCGTTCACAA
GAAATGGAAACTTTATTCTA

Downstream 100 bases:

>100_bases
GACGCATTGCTTTCCTTGTAGAAAAGTAATATGAAAAATCTTGCGCTCATTGATTTGTTTCTTAACGAATATTGGATTGA
AAAAGGGCTTTCAGAAAATA

Product: putative 3-phenylpropionic acid transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MQVRPFYWLALSFLGYYCAYGVFLPFFPAWLKSQQYGEEMIGLVIGSAYIFRFAGGLFFSSLIKKANHIIIALRLLALAS
AIIMAAMSLVAHNFWLLFIAIGLYASVNSAGMPIGDSLASTWQRQIGLDYGKVRLIGSAAFILGVVVFGGMIGWVGEQNI
VWILTALLSFYTIIQLLKPTIPPKDEIPEDSVQNDVGFIALLKNPMTLRVMIAVGLIQGSHAAYYVYSTIYWTSIGISVS
QTSLLWGIGVLAEIVLFFFSRRLFQNISISVLLYISALACVGRWAVIGYIEDFWLIFLLQLMHSLTYAVCHYAIVRYITT
QPQSHIAKLQGLYNGLSNGVLIAIFTAIAGMIYPTSPAMTFVFMSIIAAAAFFVIPRKLNAFLIVQHK

Sequences:

>Translated_388_residues
MQVRPFYWLALSFLGYYCAYGVFLPFFPAWLKSQQYGEEMIGLVIGSAYIFRFAGGLFFSSLIKKANHIIIALRLLALAS
AIIMAAMSLVAHNFWLLFIAIGLYASVNSAGMPIGDSLASTWQRQIGLDYGKVRLIGSAAFILGVVVFGGMIGWVGEQNI
VWILTALLSFYTIIQLLKPTIPPKDEIPEDSVQNDVGFIALLKNPMTLRVMIAVGLIQGSHAAYYVYSTIYWTSIGISVS
QTSLLWGIGVLAEIVLFFFSRRLFQNISISVLLYISALACVGRWAVIGYIEDFWLIFLLQLMHSLTYAVCHYAIVRYITT
QPQSHIAKLQGLYNGLSNGVLIAIFTAIAGMIYPTSPAMTFVFMSIIAAAAFFVIPRKLNAFLIVQHK
>Mature_388_residues
MQVRPFYWLALSFLGYYCAYGVFLPFFPAWLKSQQYGEEMIGLVIGSAYIFRFAGGLFFSSLIKKANHIIIALRLLALAS
AIIMAAMSLVAHNFWLLFIAIGLYASVNSAGMPIGDSLASTWQRQIGLDYGKVRLIGSAAFILGVVVFGGMIGWVGEQNI
VWILTALLSFYTIIQLLKPTIPPKDEIPEDSVQNDVGFIALLKNPMTLRVMIAVGLIQGSHAAYYVYSTIYWTSIGISVS
QTSLLWGIGVLAEIVLFFFSRRLFQNISISVLLYISALACVGRWAVIGYIEDFWLIFLLQLMHSLTYAVCHYAIVRYITT
QPQSHIAKLQGLYNGLSNGVLIAIFTAIAGMIYPTSPAMTFVFMSIIAAAAFFVIPRKLNAFLIVQHK

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily

Homologues:

Organism=Escherichia coli, GI1788886, Length=377, Percent_Identity=35.8090185676393, Blast_Score=221, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HCAT_HAEIN (P44629)

Other databases:

- EMBL:   L42023
- PIR:   F64147
- RefSeq:   NP_438475.1
- ProteinModelPortal:   P44629
- GeneID:   950679
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0308
- NMPDR:   fig|71421.1.peg.290
- TIGR:   HI_0308
- HOGENOM:   HBG551193
- OMA:   HGVTFAM
- ProtClustDB:   PRK11128
- BioCyc:   HINF71421:HI_0308-MONOMER
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005276
- TIGRFAMs:   TIGR00902

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43031; Mature: 43031

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xaf6eb7c)-; HASH(0xaf8eda0)-; HASH(0x405b277c)-; HASH(0xc97f310)-; HASH(0xc861bc4)-; HASH(0xc334b24)-; HASH(0xb627d34)-; HASH(0xb0b67e4)-; HASH(0xc23d3cc)-; HASH(0xbf98ce0)-; HASH(0xc4899f4)-; HASH(0xb0b2b48)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVRPFYWLALSFLGYYCAYGVFLPFFPAWLKSQQYGEEMIGLVIGSAYIFRFAGGLFFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIKKANHIIIALRLLALASAIIMAAMSLVAHNFWLLFIAIGLYASVNSAGMPIGDSLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TWQRQIGLDYGKVRLIGSAAFILGVVVFGGMIGWVGEQNIVWILTALLSFYTIIQLLKPT
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
IPPKDEIPEDSVQNDVGFIALLKNPMTLRVMIAVGLIQGSHAAYYVYSTIYWTSIGISVS
CCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCHH
QTSLLWGIGVLAEIVLFFFSRRLFQNISISVLLYISALACVGRWAVIGYIEDFWLIFLLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMHSLTYAVCHYAIVRYITTQPQSHIAKLQGLYNGLSNGVLIAIFTAIAGMIYPTSPAMT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHH
FVFMSIIAAAAFFVIPRKLNAFLIVQHK
HHHHHHHHHHHHHHHHHCCCEEEEEEEH
>Mature Secondary Structure
MQVRPFYWLALSFLGYYCAYGVFLPFFPAWLKSQQYGEEMIGLVIGSAYIFRFAGGLFFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIKKANHIIIALRLLALASAIIMAAMSLVAHNFWLLFIAIGLYASVNSAGMPIGDSLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TWQRQIGLDYGKVRLIGSAAFILGVVVFGGMIGWVGEQNIVWILTALLSFYTIIQLLKPT
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
IPPKDEIPEDSVQNDVGFIALLKNPMTLRVMIAVGLIQGSHAAYYVYSTIYWTSIGISVS
CCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCHH
QTSLLWGIGVLAEIVLFFFSRRLFQNISISVLLYISALACVGRWAVIGYIEDFWLIFLLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMHSLTYAVCHYAIVRYITTQPQSHIAKLQGLYNGLSNGVLIAIFTAIAGMIYPTSPAMT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHH
FVFMSIIAAAAFFVIPRKLNAFLIVQHK
HHHHHHHHHHHHHHHHHCCCEEEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800