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The map label for this gene is ldhA

Identifier: 16272059

GI number: 16272059

Start: 89182

End: 90177

Strand: Reverse

Name: ldhA

Synonym: HI0085

Alternate gene names: 16272059

Gene position: 90177-89182 (Counterclockwise)

Preceding gene: 16272060

Following gene: 16272058

Centisome position: 4.93

GC content: 39.16

Gene sequence:

>996_bases
ATGAAAATTGCAATCTATAGCACTAAAAGTTATGACAGAAAATATATTGAGCTCATTAACGCGAAATATAATTTCGATTT
AGAATTTTTTGATTTTATGTTGAACGAGAGCACTGTGCGTTTGGCAGAACATTGCGAAGTAGTATGCATTTTCGTGAATG
ATAATGGTAGCCGAAAAGTATTAGAAAAATTAGCCGCACTTGGTGTGAAAATTGTGGCTTTGCGCTGTGCCGGTTTCAAT
AATGTTGATTTAAAAGCAGCTCAAGAATTAGGCATCCAAGTTGTCCGCGTTCCAGCTTATTCGCCTGAAGCGGTGGCGGA
ACATACCATCGGTTTGATGATGACTCTGAACCGTCGAATTCATCGTGCTTATCAGCGTACCCGAGAAGCGAATTTCTCTC
TTGAGGGGTTAATTGGTTTCAATATGTACGGACGCACAGTCGGCGTAATCGGAACAGGAAAAATCGGGATCGCAGTGATG
CGAATTTTGAAAGGTTTTGGCATGAATATCTTGGCGTACGATCCTTTCAAAAATCCAGTAGTAGAAGAACTGGGGGGACA
ATATGTTGAATTAGATGAACTTTATGCTAAATCTCATGTGATTACGCTTCATTGCCCAGCAACGCCAGAAAACTATCATT
TATTAAATTGCGAGGCTTTTGCAAAAATGAAAGATGGCGTGATGATCGTTAATACGAGTCGCGGTTCTTTGATTGATACT
CAAGCTGCGATTGATGCGTTAAAACAGCGTAAAATCGGTGCGTTAGGTATGGATGTTTACGAGAATGAGCGTGATTTATT
CTTTGAAGATAAGTCTAACGAAGTGATTCAAGATGATATTTTCCGCCGTTTATCTTCTTGCCATAATGTATTGCTAACAG
GTCACCAAGCGTTTTTAACCGAAGAAGCACTAACCAATATTGCAGACGTGACTTTATCAAACATTTATAAATTGAAATCG
GGTAAAGTGTGTGAAAATATTGTTTTGCCGTCTTAG

Upstream 100 bases:

>100_bases
TAATACCTTGTTGCGTTTTTCAGTCGGTATTGAAGATGTAGAAGATATTAAAGCGGACTTATTACAAGCTTTTGCAAACT
TAAAATAATTGGAGCAAACC

Downstream 100 bases:

>100_bases
TTTTCGGCAATTAAAACGATTAGTTTACATTTTTTAAAACGGTGTTATTCTGCACCGCACTTGTAAGTTTAAAAAAAGCC
CTTATATAGGGATTCCATTC

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKVLEKLAALGVKIVALRCAGFN
NVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRIHRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVM
RILKGFGMNILAYDPFKNPVVEELGGQYVELDELYAKSHVITLHCPATPENYHLLNCEAFAKMKDGVMIVNTSRGSLIDT
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLTEEALTNIADVTLSNIYKLKS
GKVCENIVLPS

Sequences:

>Translated_331_residues
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKVLEKLAALGVKIVALRCAGFN
NVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRIHRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVM
RILKGFGMNILAYDPFKNPVVEELGGQYVELDELYAKSHVITLHCPATPENYHLLNCEAFAKMKDGVMIVNTSRGSLIDT
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLTEEALTNIADVTLSNIYKLKS
GKVCENIVLPS
>Mature_331_residues
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKVLEKLAALGVKIVALRCAGFN
NVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRIHRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVM
RILKGFGMNILAYDPFKNPVVEELGGQYVELDELYAKSHVITLHCPATPENYHLLNCEAFAKMKDGVMIVNTSRGSLIDT
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLTEEALTNIADVTLSNIYKLKS
GKVCENIVLPS

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Homologues:

Organism=Homo sapiens, GI61743967, Length=309, Percent_Identity=32.3624595469256, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI4557497, Length=309, Percent_Identity=32.3624595469256, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI145580578, Length=280, Percent_Identity=31.7857142857143, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557499, Length=280, Percent_Identity=31.7857142857143, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI145580575, Length=280, Percent_Identity=31.7857142857143, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI23308577, Length=212, Percent_Identity=33.9622641509434, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI6912396, Length=223, Percent_Identity=29.5964125560538, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=70.8588957055215, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI87082289, Length=286, Percent_Identity=29.7202797202797, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789279, Length=247, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=277, Percent_Identity=30.6859205776173, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=238, Percent_Identity=28.5714285714286, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=235, Percent_Identity=34.8936170212766, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324055, Length=211, Percent_Identity=36.0189573459716, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=235, Percent_Identity=34.0425531914894, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324964, Length=216, Percent_Identity=26.3888888888889, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=125, Percent_Identity=32, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321253, Length=189, Percent_Identity=27.5132275132275, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24646446, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24646448, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24646452, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24646450, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI62472511, Length=307, Percent_Identity=31.9218241042345, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=30.7053941908714, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28574286, Length=277, Percent_Identity=29.2418772563177, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45552429, Length=269, Percent_Identity=26.7657992565056, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585514, Length=269, Percent_Identity=26.7657992565056, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574282, Length=269, Percent_Identity=26.7657992565056, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574284, Length=269, Percent_Identity=26.7657992565056, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45551003, Length=269, Percent_Identity=26.7657992565056, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571528, Length=275, Percent_Identity=26.5454545454545, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDH_HAEIN (P44501)

Other databases:

- EMBL:   L42023
- PIR:   F64047
- RefSeq:   NP_438258.1
- ProteinModelPortal:   P44501
- SMR:   P44501
- GeneID:   950977
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0085
- NMPDR:   fig|71421.1.peg.84
- TIGR:   HI_0085
- HOGENOM:   HBG731446
- OMA:   AFDKMKD
- ProtClustDB:   CLSK869554
- BioCyc:   HINF71421:HI_0085-MONOMER
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: 1.1.1.28

Molecular weight: Translated: 37088; Mature: 37088

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 234-234 ACT_SITE 263-263 ACT_SITE 295-295 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKV
CEEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCHHH
LEKLAALGVKIVALRCAGFNNVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRI
HHHHHHCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVMRILKGFGMNILAYDPFKNPV
HHHHHHHHHCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHH
VEELGGQYVELDELYAKSHVITLHCPATPENYHLLNCEAFAKMKDGVMIVNTSRGSLIDT
HHHHCCCEEEHHHHHHCCEEEEEECCCCCCCEEEEEHHHHHHCCCCEEEEECCCCCEEHH
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLT
HHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCHHHHH
EEALTNIADVTLSNIYKLKSGKVCENIVLPS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIAIYSTKSYDRKYIELINAKYNFDLEFFDFMLNESTVRLAEHCEVVCIFVNDNGSRKV
CEEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCHHH
LEKLAALGVKIVALRCAGFNNVDLKAAQELGIQVVRVPAYSPEAVAEHTIGLMMTLNRRI
HHHHHHCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYQRTREANFSLEGLIGFNMYGRTVGVIGTGKIGIAVMRILKGFGMNILAYDPFKNPV
HHHHHHHHHCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHH
VEELGGQYVELDELYAKSHVITLHCPATPENYHLLNCEAFAKMKDGVMIVNTSRGSLIDT
HHHHCCCEEEHHHHHHCCEEEEEECCCCCCCEEEEEHHHHHHCCCCEEEEECCCCCEEHH
QAAIDALKQRKIGALGMDVYENERDLFFEDKSNEVIQDDIFRRLSSCHNVLLTGHQAFLT
HHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEECCHHHHH
EEALTNIADVTLSNIYKLKSGKVCENIVLPS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800