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The map label for this gene is metB [H]

Identifier: 16272060

GI number: 16272060

Start: 90190

End: 91335

Strand: Reverse

Name: metB [H]

Synonym: HI0086

Alternate gene names: 16272060

Gene position: 91335-90190 (Counterclockwise)

Preceding gene: 16272061

Following gene: 16272059

Centisome position: 4.99

GC content: 38.31

Gene sequence:

>1146_bases
GTGCGGTCAATTTTTTCATTATTTTTAGAGGATGTTATGACACAACAATATGCTATCGATACTTTATTAGCTCAAGCAGG
CAATCGCAGTGATGAGCGTACGGGCGCGGTTTCTGCACCAATTTTTCTTTCTACGGCTTATGGTCATTGTGGTATCGGTG
AAAGTACGGGATTTGATTACACACGTACCAAAAATCCAACTCGCACAGTATTAGAAGAAACGATAGCTAAATTAGAGAAT
GGAGATCGTGGTTTTGCGTTTTCTTCTGGTATGGCTGCAATTCAAGTTTTGATGACGCTTTTTACCGCCCCAGATGAATG
GATTGTTTCTAGCGATGTGTATGGTGGTACTTATCGATTATTAGATTTTTCTTATAAAAATAACAATAGTGTAAAACCTG
TTTATGTTAATACAGCATCTGCTTCAGCAATTGAGGCTGCCATTAACCCAAATACGAAAGCGATTTTTATTGAAACCCCA
TCGAATCCTTTAATGGAAGAATGTGATGTGGTTGAAATTGCAAAACTTGCGAAAAAACATAACTTAATGTTGATCGTGGA
TAATACGTTTTTAACGCCTGTGCTTTCTCGTCCATTAGATTTAGGTGCGGATGTGGTAATTCACAGCGGAACTAAATATA
TTGCTGGGCATAATGATGCACTTGTTGGTTTAATTGTTGCGAAAGGGCAAGAACTTTGTGATCGCATTGCTTATATTCAA
AATGGTGCAGGGGCGGTGCTTTCTCCTTTTGATTCTTGGCTGACTATTCGTGGTATGAAGACCCTTTCTTTGCGTATGAA
ACGCCATCAAGAAAATGCACAAGCTATTGCGGAATTTTTAAAAGCTCAACCACAAGTGGAATCGGTGCTTTATCCGAATA
AAGGTGGGATGTTGTCTTTCCGTTTGCAAGATGAAGCTTGGGTTAATACATTTTTAAAATCCATCAAATTGATTACTTTT
GCAGAAAGTCTAGGCGGCACAGAAAGTTTTATTACTTATCCTGCCACTCAAACTCATATGGATATTCCAGAATCCGAGCG
TGTAGCTCGTGGGATTACTAATACCTTGTTGCGTTTTTCAGTCGGTATTGAAGATGTAGAAGATATTAAAGCGGACTTAT
TACAAGCTTTTGCAAACTTAAAATAA

Upstream 100 bases:

>100_bases
AGAAAATGATGGAAAAATGAAATTTTATGTAATTTTCTAATTTCTACTTTTATCACTTTTTCCTTTGTGCCAAAATGCTA
AACGTTTTTTAACACGTAAA

Downstream 100 bases:

>100_bases
TTGGAGCAAACCATGAAAATTGCAATCTATAGCACTAAAAGTTATGACAGAAAATATATTGAGCTCATTAACGCGAAATA
TAATTTCGATTTAGAATTTT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRSIFSLFLEDVMTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLEETIAKLEN
GDRGFAFSSGMAAIQVLMTLFTAPDEWIVSSDVYGGTYRLLDFSYKNNNSVKPVYVNTASASAIEAAINPNTKAIFIETP
SNPLMEECDVVEIAKLAKKHNLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQ
NGAGAVLSPFDSWLTIRGMKTLSLRMKRHQENAQAIAEFLKAQPQVESVLYPNKGGMLSFRLQDEAWVNTFLKSIKLITF
AESLGGTESFITYPATQTHMDIPESERVARGITNTLLRFSVGIEDVEDIKADLLQAFANLK

Sequences:

>Translated_381_residues
MRSIFSLFLEDVMTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLEETIAKLEN
GDRGFAFSSGMAAIQVLMTLFTAPDEWIVSSDVYGGTYRLLDFSYKNNNSVKPVYVNTASASAIEAAINPNTKAIFIETP
SNPLMEECDVVEIAKLAKKHNLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQ
NGAGAVLSPFDSWLTIRGMKTLSLRMKRHQENAQAIAEFLKAQPQVESVLYPNKGGMLSFRLQDEAWVNTFLKSIKLITF
AESLGGTESFITYPATQTHMDIPESERVARGITNTLLRFSVGIEDVEDIKADLLQAFANLK
>Mature_381_residues
MRSIFSLFLEDVMTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLEETIAKLEN
GDRGFAFSSGMAAIQVLMTLFTAPDEWIVSSDVYGGTYRLLDFSYKNNNSVKPVYVNTASASAIEAAINPNTKAIFIETP
SNPLMEECDVVEIAKLAKKHNLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQ
NGAGAVLSPFDSWLTIRGMKTLSLRMKRHQENAQAIAEFLKAQPQVESVLYPNKGGMLSFRLQDEAWVNTFLKSIKLITF
AESLGGTESFITYPATQTHMDIPESERVARGITNTLLRFSVGIEDVEDIKADLLQAFANLK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=345, Percent_Identity=40.2898550724638, Blast_Score=264, Evalue=9e-71,
Organism=Homo sapiens, GI299473759, Length=345, Percent_Identity=36.5217391304348, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI25453487, Length=344, Percent_Identity=35.4651162790698, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=40.2088772845953, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=29.5739348370927, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538055, Length=347, Percent_Identity=42.3631123919308, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17560056, Length=347, Percent_Identity=42.3631123919308, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI71980708, Length=392, Percent_Identity=33.9285714285714, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=378, Percent_Identity=39.6825396825397, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=30.7692307692308, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=31.4814814814815, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=282, Percent_Identity=31.5602836879433, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=386, Percent_Identity=25.3886010362694, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19922554, Length=373, Percent_Identity=39.6782841823056, Blast_Score=278, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41810; Mature: 41810

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIFSLFLEDVMTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
TRTKNPTRTVLEETIAKLENGDRGFAFSSGMAAIQVLMTLFTAPDEWIVSSDVYGGTYRL
CCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEE
LDFSYKNNNSVKPVYVNTASASAIEAAINPNTKAIFIETPSNPLMEECDVVEIAKLAKKH
EEEEECCCCCCCEEEEECCCCHHEEEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHHC
NLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQ
CEEEEECCCHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCHHHHHHHHHHH
NGAGAVLSPFDSWLTIRGMKTLSLRMKRHQENAQAIAEFLKAQPQVESVLYPNKGGMLSF
CCCCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCEEEE
RLQDEAWVNTFLKSIKLITFAESLGGTESFITYPATQTHMDIPESERVARGITNTLLRFS
EECHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
VGIEDVEDIKADLLQAFANLK
CCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSIFSLFLEDVMTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
TRTKNPTRTVLEETIAKLENGDRGFAFSSGMAAIQVLMTLFTAPDEWIVSSDVYGGTYRL
CCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEE
LDFSYKNNNSVKPVYVNTASASAIEAAINPNTKAIFIETPSNPLMEECDVVEIAKLAKKH
EEEEECCCCCCCEEEEECCCCHHEEEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHHC
NLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQ
CEEEEECCCHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCHHHHHHHHHHH
NGAGAVLSPFDSWLTIRGMKTLSLRMKRHQENAQAIAEFLKAQPQVESVLYPNKGGMLSF
CCCCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCEEEE
RLQDEAWVNTFLKSIKLITFAESLGGTESFITYPATQTHMDIPESERVARGITNTLLRFS
EECHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
VGIEDVEDIKADLLQAFANLK
CCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]