Click here to switch to the map view.

The map label for this gene is fdxH

Identifier: 16271982

GI number: 16271982

Start: 8750

End: 9688

Strand: Direct

Name: fdxH

Synonym: HI0007

Alternate gene names: 16271982

Gene position: 8750-9688 (Clockwise)

Preceding gene: 16271978

Following gene: 16271983

Centisome position: 0.48

GC content: 41.43

Gene sequence:

>939_bases
ATGGCTGGAACTGCTCAAGGCGTTCAAACGCAAGACGTTATTAAAATCTCCGCAACATCTGGTTTAACACCAGCACCACA
AGCGAGAGATCATAAAGTTGAAATCGCAAAATTAATTGATGTTTCAACTTGTATAGGATGTAAAGCCTGTCAAGTGGGTT
GTTCAGAGTGGAATGATATTCGCTCTGATATCAATGCACAATGTGTTGGGGTTTATGACAATCCAGTTGATCTTGATGCA
AAAGCATGGACAGTGATGCGTTTTAACGAAGTTGAAGAAAACGATCGTTTAGAATGGCTGATTCGTAAAGATGGCTGTAT
GCACTGCACAGAACCTGGTTGTTTAAAGGCTTGTCCTGCACCCGGTGCAATCATTCAATATGCTAACGGTATTGTAGATT
TCCAATCCGATAAATGTATTGGTTGTGGTTATTGTATTGCAGGCTGTCCATTCAATATTCCACGAATGAATCCTGAAGAC
AATCGTGTATACAAATGTACTCTTTGTGTAGATCGTGTTTCTGTGGGACAAGAGCCCGCTTGCGTGAAAACCTGTCCAAC
AGGTGCTATTCGTTTCGGCTCTAAAGAAGAAATGAAAATTTACGCAGAACAACGCGTGGCAGATTTAAAATCCCGTGGTT
ATGAAAACGCAGGACTTTACGATCCTCCAGGTGTAGGCGGTACACACGTAATGTATGTGTTGCATCACGCAGATAAACCT
GAGCTTTACAATGGTCTTCCAAAAGATCCTCAAATTGATTTGAGTGTGACCTTATGGAAAGATGTATTGAAACCAGTCGC
TGCAGTTGCAATGGGTGGTCTAGCTCTAGCAGAAGTTGCGCACTACTTAACCGTTGGCCCAAATGTAGAAGAAGATGTGG
AAGATCATCATCACGAATTTGAAGAAAATAAACCATCTAAGGGGGAAAATAATGAGTAA

Upstream 100 bases:

>100_bases
TCTACGAATACCTTAACACCATCTTGGGGTGAGGCAATCACGCAAACACCAGAATACAAAACATTCTTGGTAAATATTGA
AAAAGTTGGGGAGGCATAAT

Downstream 100 bases:

>100_bases
AATTGAAATTAGCAACGATACTCGCATTATCCGCCATAGAACCCCAGCACGTATCAGTCACTGGATGTTGGTTATTTGCT
TTTTTATGACGATGTTCACT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MAGTAQGVQTQDVIKISATSGLTPAPQARDHKVEIAKLIDVSTCIGCKACQVGCSEWNDIRSDINAQCVGVYDNPVDLDA
KAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPED
NRVYKCTLCVDRVSVGQEPACVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
ELYNGLPKDPQIDLSVTLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEFEENKPSKGENNE

Sequences:

>Translated_312_residues
MAGTAQGVQTQDVIKISATSGLTPAPQARDHKVEIAKLIDVSTCIGCKACQVGCSEWNDIRSDINAQCVGVYDNPVDLDA
KAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPED
NRVYKCTLCVDRVSVGQEPACVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
ELYNGLPKDPQIDLSVTLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEFEENKPSKGENNE
>Mature_311_residues
AGTAQGVQTQDVIKISATSGLTPAPQARDHKVEIAKLIDVSTCIGCKACQVGCSEWNDIRSDINAQCVGVYDNPVDLDAK
AWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPEDN
RVYKCTLCVDRVSVGQEPACVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKPE
LYNGLPKDPQIDLSVTLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEFEENKPSKGENNE

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1790326, Length=291, Percent_Identity=73.1958762886598, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1787749, Length=293, Percent_Identity=73.7201365187713, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1789370, Length=273, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787122, Length=162, Percent_Identity=32.7160493827161, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI2367345, Length=183, Percent_Identity=30.0546448087432, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI226510944, Length=219, Percent_Identity=26.4840182648402, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=28.9017341040462, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=36, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787740, Length=100, Percent_Identity=36, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787963, Length=176, Percent_Identity=24.4318181818182, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87082180, Length=185, Percent_Identity=27.5675675675676, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FDXH_HAEIN (P44450)

Other databases:

- EMBL:   L42023
- PIR:   A64042
- RefSeq:   NP_438180.1
- ProteinModelPortal:   P44450
- SMR:   P44450
- GeneID:   950904
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0007
- NMPDR:   fig|71421.1.peg.6
- TIGR:   HI_0007
- HOGENOM:   HBG726839
- OMA:   TSISLWK
- ProtClustDB:   CLSK870084
- BioCyc:   HINF71421:HI_0007-MONOMER
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246
- PIRSF:   PIRSF036298
- TIGRFAMs:   TIGR01582

Pfam domain/function: PF09163 Form-deh_trans

EC number: 1.2.1.2

Molecular weight: Translated: 34069; Mature: 33938

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTAQGVQTQDVIKISATSGLTPAPQARDHKVEIAKLIDVSTCIGCKACQVGCSEWNDI
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
RSDINAQCVGVYDNPVDLDAKAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPA
HHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
PGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPEDNRVYKCTLCVDRVSVGQEPA
CCHHHHHHCCEEEECCCCEEECCCEECCCCCCCCCCCCCCCCEEEEEEEEEHHHCCCCCC
CVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
EEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ELYNGLPKDPQIDLSVTLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEF
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EENKPSKGENNE
HCCCCCCCCCCC
>Mature Secondary Structure 
AGTAQGVQTQDVIKISATSGLTPAPQARDHKVEIAKLIDVSTCIGCKACQVGCSEWNDI
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
RSDINAQCVGVYDNPVDLDAKAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPA
HHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
PGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPEDNRVYKCTLCVDRVSVGQEPA
CCHHHHHHCCEEEECCCCEEECCCEECCCCCCCCCCCCCCCCEEEEEEEEEHHHCCCCCC
CVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
EEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ELYNGLPKDPQIDLSVTLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEF
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EENKPSKGENNE
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800