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The map label for this gene is purB [H]

Identifier: 162448170

GI number: 162448170

Start: 1390334

End: 1391626

Strand: Direct

Name: purB [H]

Synonym: ACL_1332

Alternate gene names: 162448170

Gene position: 1390334-1391626 (Clockwise)

Preceding gene: 162448166

Following gene: 162448205

Centisome position: 92.88

GC content: 33.18

Gene sequence:

>1293_bases
ATGATAAAAAGATATCGAACACCATTATTTGAAACTTTATGGAGTGATCAAAATCGCTATGACACTTTTTTGACAATTGA
ACTAGCTGTTGCCAAAGCGTGGTATTTATTAGGCAAATATGATGAAACAACTTACAATAAACTTACTAAAGCAACCTTTG
ATTTAGAATCAATCTATGCATTAGAAATAGAAACAAAACATGATATGATTGCATTTACAAGAACTGTCTCTAAAAGTTTG
GGTGCTGAGAAAATGTGGCTACACTATGGTTTAACATCTACAGATGTTGTCGACAGTGCACTAGCACTTATACTCAGGCA
AGCCAATCAAGCAATTAGATCAAAAATTAATAAGTTATTGTCAGTCTTAAAACAAAAAGCTTATACTTATAAAGACCAAC
CAACCATAGGTCGTACCCATGGCATCCACGCTGAAATTACATCTTTTGGATTAAAATTTGCACTATGGTACAGTGATCTA
AAACGGCTACTTTCTGTATTTGATGCTGCCTGTAAAAACATCGAAGTGATTAAAATATCAGGCGCTGTTGGCAACTATGC
GGCCAACACACCTCAACTAGAAAAAACAGTTTCCCGTCTATTAGATATAGCATCTGCAGACATTTCTACACAAGTACTTC
AAAGAGACAGACATGCAAACTACATTTCAAGTATTGCACTATTGGGTAGTGAACTAGAAAAAATAGCCACTGAAATTAGA
CATTTATCACGTACTGAAGTATCTGAGGTAAATGAATACTTCAGTGTAGATCAAAAAGGTTCTAGTGCAATGCCACATAA
AAAAAATCCGGTATCTAGTGAAAATATATCTGGATTATCTAGAGTCTTAAGAGGTTATGTTTTAACAGCTTTTGAAAATA
TTAATTTATGGCATGAAAGAGATATTTCCCACTCCTCTGCTGAGCGTATCATATTAGCAGATGCAACAACATTAATTGAT
TATATGTTAGATCGTATAACAAATACGCTATCTAACTTAGAGGTCCACGCATTAAAACTACAAAACAATATAGATATAAC
TGGTGGTATCGTATTTAGTCAACATGTACTATCTTTATTGATTGATAAAGGACTTACAAGAGATCATGCGTATGATTTGG
TTCAAAAGATTGCGACTGATGCCATGAGCAATCAAAAAGATTTTAAAACAGAACTTTTAAAAAGTTCAGATATCATGAAG
TTTATGACACCAGATGAACTGGATAAAGCGTTTGACTCAATCTATATATTAAGACATGTCGATACCATATACTTAAAAGT
CTTTAAAACATAA

Upstream 100 bases:

>100_bases
CAAAATTACAGAACTTACAAAAATACCATTAGCTATATTTTCAGTGGGTCCAAGGCGTGACCAAACAATAGAAGTATCAT
CTATATTTGAGGATTAATTT

Downstream 100 bases:

>100_bases
TAAAAGTCACATATCTATTTGATGTGTGACTTTTATTATCCCTTTACTTTAGGATTTTTTTGATTATATTTTCTTTGGAC
TTTGTATATGGTGGATAGAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIKRYRTPLFETLWSDQNRYDTFLTIELAVAKAWYLLGKYDETTYNKLTKATFDLESIYALEIETKHDMIAFTRTVSKSL
GAEKMWLHYGLTSTDVVDSALALILRQANQAIRSKINKLLSVLKQKAYTYKDQPTIGRTHGIHAEITSFGLKFALWYSDL
KRLLSVFDAACKNIEVIKISGAVGNYAANTPQLEKTVSRLLDIASADISTQVLQRDRHANYISSIALLGSELEKIATEIR
HLSRTEVSEVNEYFSVDQKGSSAMPHKKNPVSSENISGLSRVLRGYVLTAFENINLWHERDISHSSAERIILADATTLID
YMLDRITNTLSNLEVHALKLQNNIDITGGIVFSQHVLSLLIDKGLTRDHAYDLVQKIATDAMSNQKDFKTELLKSSDIMK
FMTPDELDKAFDSIYILRHVDTIYLKVFKT

Sequences:

>Translated_430_residues
MIKRYRTPLFETLWSDQNRYDTFLTIELAVAKAWYLLGKYDETTYNKLTKATFDLESIYALEIETKHDMIAFTRTVSKSL
GAEKMWLHYGLTSTDVVDSALALILRQANQAIRSKINKLLSVLKQKAYTYKDQPTIGRTHGIHAEITSFGLKFALWYSDL
KRLLSVFDAACKNIEVIKISGAVGNYAANTPQLEKTVSRLLDIASADISTQVLQRDRHANYISSIALLGSELEKIATEIR
HLSRTEVSEVNEYFSVDQKGSSAMPHKKNPVSSENISGLSRVLRGYVLTAFENINLWHERDISHSSAERIILADATTLID
YMLDRITNTLSNLEVHALKLQNNIDITGGIVFSQHVLSLLIDKGLTRDHAYDLVQKIATDAMSNQKDFKTELLKSSDIMK
FMTPDELDKAFDSIYILRHVDTIYLKVFKT
>Mature_430_residues
MIKRYRTPLFETLWSDQNRYDTFLTIELAVAKAWYLLGKYDETTYNKLTKATFDLESIYALEIETKHDMIAFTRTVSKSL
GAEKMWLHYGLTSTDVVDSALALILRQANQAIRSKINKLLSVLKQKAYTYKDQPTIGRTHGIHAEITSFGLKFALWYSDL
KRLLSVFDAACKNIEVIKISGAVGNYAANTPQLEKTVSRLLDIASADISTQVLQRDRHANYISSIALLGSELEKIATEIR
HLSRTEVSEVNEYFSVDQKGSSAMPHKKNPVSSENISGLSRVLRGYVLTAFENINLWHERDISHSSAERIILADATTLID
YMLDRITNTLSNLEVHALKLQNNIDITGGIVFSQHVLSLLIDKGLTRDHAYDLVQKIATDAMSNQKDFKTELLKSSDIMK
FMTPDELDKAFDSIYILRHVDTIYLKVFKT

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:
GO:0003824: Adenylosuccinate lyase
GO:0004018: Adenylosuccinate lyase
GO:0009152: Adenylosuccinate lyase
GO:0016829: Adenylosuccinate lyase
GO:0070626: Adenylosuccinate lyase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=431, Percent_Identity=26.6821345707657, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.0839895013123, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787376, Length=314, Percent_Identity=25.796178343949, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=382, Percent_Identity=25.9162303664921, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=314, Percent_Identity=27.0700636942675, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=434, Percent_Identity=25.3456221198157, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647570, Length=402, Percent_Identity=25.6218905472637, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48788; Mature: 48788

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYRTPLFETLWSDQNRYDTFLTIELAVAKAWYLLGKYDETTYNKLTKATFDLESIYA
CCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEE
LEIETKHDMIAFTRTVSKSLGAEKMWLHYGLTSTDVVDSALALILRQANQAIRSKINKLL
EEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVLKQKAYTYKDQPTIGRTHGIHAEITSFGLKFALWYSDLKRLLSVFDAACKNIEVIKIS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GAVGNYAANTPQLEKTVSRLLDIASADISTQVLQRDRHANYISSIALLGSELEKIATEIR
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSRTEVSEVNEYFSVDQKGSSAMPHKKNPVSSENISGLSRVLRGYVLTAFENINLWHER
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHC
DISHSSAERIILADATTLIDYMLDRITNTLSNLEVHALKLQNNIDITGGIVFSQHVLSLL
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEECHHHHHHHHHHHH
IDKGLTRDHAYDLVQKIATDAMSNQKDFKTELLKSSDIMKFMTPDELDKAFDSIYILRHV
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
DTIYLKVFKT
HEEHEEEECC
>Mature Secondary Structure
MIKRYRTPLFETLWSDQNRYDTFLTIELAVAKAWYLLGKYDETTYNKLTKATFDLESIYA
CCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEE
LEIETKHDMIAFTRTVSKSLGAEKMWLHYGLTSTDVVDSALALILRQANQAIRSKINKLL
EEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVLKQKAYTYKDQPTIGRTHGIHAEITSFGLKFALWYSDLKRLLSVFDAACKNIEVIKIS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GAVGNYAANTPQLEKTVSRLLDIASADISTQVLQRDRHANYISSIALLGSELEKIATEIR
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSRTEVSEVNEYFSVDQKGSSAMPHKKNPVSSENISGLSRVLRGYVLTAFENINLWHER
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHC
DISHSSAERIILADATTLIDYMLDRITNTLSNLEVHALKLQNNIDITGGIVFSQHVLSLL
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEECHHHHHHHHHHHH
IDKGLTRDHAYDLVQKIATDAMSNQKDFKTELLKSSDIMKFMTPDELDKAFDSIYILRHV
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
DTIYLKVFKT
HEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA