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The map label for this gene is priA [H]

Identifier: 162447190

GI number: 162447190

Start: 333175

End: 335439

Strand: Direct

Name: priA [H]

Synonym: ACL_0324

Alternate gene names: 162447190

Gene position: 333175-335439 (Clockwise)

Preceding gene: 162447187

Following gene: 162447191

Centisome position: 22.26

GC content: 29.62

Gene sequence:

>2265_bases
ATGATTGCTCGCGTGATTGTGGATATAAAACATCAAAGTGTGAATCAAACATTTGATTATTTAGTTCACGAAAAAGATCA
ACTTCATATTCAAAAAGGTATGCGTGTAATTGTACCTTTTACAGATAATAATATATTACGTTTAGGTTTTGTCTATGATA
TTATAAGTGCATCTGATTTAGCACATAAGTATGTTCATGAAATATTAGACATTGAACCTATTTTTAATGATGAATTATTT
TTAATGATGGATAAACTGATAGAAGACCCAAATGCACTCATTGCAGAAGCCTTTGAAACCGTGATACCTAAACAATTATT
AATTCATTATGAGAAAAAGGCTACTTTGTTAGAACCTAAACAACTACCGGATGATTTAGTGCCGTTTTTCAAACAAGGTG
TTTGGACTTTGCTTAAAAAAGATGAAGTTTATTATAACCGTTTAAAAACATTAGAACAAAAAGGTATAGTTGAATTAAAA
ACCGTATTAAAGACAAGAAATAAGAAACCTTTAATAACCTATGTAAGAATCGATAACAGAAACTATCATGGTACACCAAA
ACAACACGAAGTACTAGATATTTTAAGTGCGCACTCACAAATGAAAAAAGCAGATCTCGTAGCAAACTCTAGTGCATCGA
TTGTAAATACGTTAATCAAAAAAGCTGTTATTGAAGTATTTTATGAATCAGATAAGTTAGAAGTAGCTAATTTTAACAAA
ACAACAAAACTACCTGTGATTAATGAAGATTTAAAAGACCAAATCAAAGATATGATGGATGCAAAACATCAAGTTTATGT
GAGTCAGTATCAAGATACCAAGTTAGACCCTTTCTTTTTCCATTTGATTCATGAAGTGATTTCACAGGGTAAGCAAGTGC
TTATCATGGTGCCTGAAAACTTTATGATTGCTTCTGTTAAGTCATCACTTGAAAAAGTCTTTAGGGAAGAACTTATTATT
GGTTTAAATCGTGGTCAAACAGATAAACAAATGATGTTAAAGTACAGTGCAATTTTAGATAATGAAACTAAAATTGTCGT
CGGTGCACGTTCATCTGTATTTACAAGTTTTAGTGATCTAGGTTTAATCATCATAACGGATAGTAATAATCACAGTTACC
GTGCACATGAAGGTATTTATTATCATGCATTAGAGATTGCTGAAATAAGAGCTAGATATCATGGTATACCACTTTATTTA
AGTGCGAGTTCAATATCCTTAGATAACTATGTTAAGATACAACAAAAAACACATGAGTTACTAAATCTACAAGAAGAAGT
CTATAAATCCATTGAACTTATAGATATGAAAGAAGAACTAAAAAAAGGAAATACCAAATTAGTTAGTCTAAGTTTAAATG
ATGCCATACTAAATACATTAGATCAAAATAAAAAAGTATTATTAATATTAAACCAAAAAGGGTATGCACCCTTTGTAATG
TGTAGGACATGTTCGCATGTACCTGTAGATCCAGAAACTGGAATCCCTTTAAGATTTGATGAAAAACAAAATATTTTAAA
ATCTAATTTGACAAAATATCAACTGGAGTTTACTAAAACATGCCCTGTATGCGGTAAACATACAGTGAAGTCTGTAGGTA
GTGGTATTGATCAACTTATAACTTACTTACATAAAGCATATCCTACGAAAGAGATATTAAAAGTCGATAGTGAGGCTATT
AGTAATAAGACTTATCATGATAAAATTGAAGATCTTAGTCAAATCGATATCATTGTAGGTACACAGATGGCACTGAAATC
TAATTTAGAAGATAAAATAGATTTAGTGGGTATTTTGATGATTGATCAGTGGTTAAAATTACCCAAATTTGATGCCTATG
AAGCAACTTATGAACTACTTAGCCAAGCAAAATTTATCACTAAAGATAAACTGATTATTCAAAGTTATGATCCAACACAT
TTTGTTTTAAAAAGCATTATAGGTGATGGAAGTACATATTATAAAGAAGAACTAAGTCGTAGAAGACTTTCTAAATTACC
ACCATATTATCAAGTGCTACAACTACGCATTGAAGGTATGAGTTATTTAAAGACTTTCCAATATGCACTCTCATTAAAAA
CTTCATTAGAAAAAATGGGACTTACTGTTTTAGGTCCAACCTCATCTGTATTACTAAAACAATTAGAAAATTATAGAGTT
TTATTAACGATTAAATACCAAGGTAGTTTAAAAACGTTTAATCATTTACTAAAATCTAACAAAGACATAAAATTATATAT
GAACCATGATTTATCATGGTATTAA

Upstream 100 bases:

>100_bases
ACCACTCAACTGTATAAACCTAGCTAAACAGTGTTAAAAACACTTAAAAACTGTAAAAATGCGCATAGAAATGATATAAT
TTATTATGGTAGGTGACTAG

Downstream 100 bases:

>100_bases
AGGAGTAAATTATGATTGTATTTATGGGTACCCCAGAGTTTTCAGTACCAATTCTAGAAATGTTGATTGAAGAAAAATAT
CCGGTTGGGCTAGTTGTTAC

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MIARVIVDIKHQSVNQTFDYLVHEKDQLHIQKGMRVIVPFTDNNILRLGFVYDIISASDLAHKYVHEILDIEPIFNDELF
LMMDKLIEDPNALIAEAFETVIPKQLLIHYEKKATLLEPKQLPDDLVPFFKQGVWTLLKKDEVYYNRLKTLEQKGIVELK
TVLKTRNKKPLITYVRIDNRNYHGTPKQHEVLDILSAHSQMKKADLVANSSASIVNTLIKKAVIEVFYESDKLEVANFNK
TTKLPVINEDLKDQIKDMMDAKHQVYVSQYQDTKLDPFFFHLIHEVISQGKQVLIMVPENFMIASVKSSLEKVFREELII
GLNRGQTDKQMMLKYSAILDNETKIVVGARSSVFTSFSDLGLIIITDSNNHSYRAHEGIYYHALEIAEIRARYHGIPLYL
SASSISLDNYVKIQQKTHELLNLQEEVYKSIELIDMKEELKKGNTKLVSLSLNDAILNTLDQNKKVLLILNQKGYAPFVM
CRTCSHVPVDPETGIPLRFDEKQNILKSNLTKYQLEFTKTCPVCGKHTVKSVGSGIDQLITYLHKAYPTKEILKVDSEAI
SNKTYHDKIEDLSQIDIIVGTQMALKSNLEDKIDLVGILMIDQWLKLPKFDAYEATYELLSQAKFITKDKLIIQSYDPTH
FVLKSIIGDGSTYYKEELSRRRLSKLPPYYQVLQLRIEGMSYLKTFQYALSLKTSLEKMGLTVLGPTSSVLLKQLENYRV
LLTIKYQGSLKTFNHLLKSNKDIKLYMNHDLSWY

Sequences:

>Translated_754_residues
MIARVIVDIKHQSVNQTFDYLVHEKDQLHIQKGMRVIVPFTDNNILRLGFVYDIISASDLAHKYVHEILDIEPIFNDELF
LMMDKLIEDPNALIAEAFETVIPKQLLIHYEKKATLLEPKQLPDDLVPFFKQGVWTLLKKDEVYYNRLKTLEQKGIVELK
TVLKTRNKKPLITYVRIDNRNYHGTPKQHEVLDILSAHSQMKKADLVANSSASIVNTLIKKAVIEVFYESDKLEVANFNK
TTKLPVINEDLKDQIKDMMDAKHQVYVSQYQDTKLDPFFFHLIHEVISQGKQVLIMVPENFMIASVKSSLEKVFREELII
GLNRGQTDKQMMLKYSAILDNETKIVVGARSSVFTSFSDLGLIIITDSNNHSYRAHEGIYYHALEIAEIRARYHGIPLYL
SASSISLDNYVKIQQKTHELLNLQEEVYKSIELIDMKEELKKGNTKLVSLSLNDAILNTLDQNKKVLLILNQKGYAPFVM
CRTCSHVPVDPETGIPLRFDEKQNILKSNLTKYQLEFTKTCPVCGKHTVKSVGSGIDQLITYLHKAYPTKEILKVDSEAI
SNKTYHDKIEDLSQIDIIVGTQMALKSNLEDKIDLVGILMIDQWLKLPKFDAYEATYELLSQAKFITKDKLIIQSYDPTH
FVLKSIIGDGSTYYKEELSRRRLSKLPPYYQVLQLRIEGMSYLKTFQYALSLKTSLEKMGLTVLGPTSSVLLKQLENYRV
LLTIKYQGSLKTFNHLLKSNKDIKLYMNHDLSWY
>Mature_754_residues
MIARVIVDIKHQSVNQTFDYLVHEKDQLHIQKGMRVIVPFTDNNILRLGFVYDIISASDLAHKYVHEILDIEPIFNDELF
LMMDKLIEDPNALIAEAFETVIPKQLLIHYEKKATLLEPKQLPDDLVPFFKQGVWTLLKKDEVYYNRLKTLEQKGIVELK
TVLKTRNKKPLITYVRIDNRNYHGTPKQHEVLDILSAHSQMKKADLVANSSASIVNTLIKKAVIEVFYESDKLEVANFNK
TTKLPVINEDLKDQIKDMMDAKHQVYVSQYQDTKLDPFFFHLIHEVISQGKQVLIMVPENFMIASVKSSLEKVFREELII
GLNRGQTDKQMMLKYSAILDNETKIVVGARSSVFTSFSDLGLIIITDSNNHSYRAHEGIYYHALEIAEIRARYHGIPLYL
SASSISLDNYVKIQQKTHELLNLQEEVYKSIELIDMKEELKKGNTKLVSLSLNDAILNTLDQNKKVLLILNQKGYAPFVM
CRTCSHVPVDPETGIPLRFDEKQNILKSNLTKYQLEFTKTCPVCGKHTVKSVGSGIDQLITYLHKAYPTKEILKVDSEAI
SNKTYHDKIEDLSQIDIIVGTQMALKSNLEDKIDLVGILMIDQWLKLPKFDAYEATYELLSQAKFITKDKLIIQSYDPTH
FVLKSIIGDGSTYYKEELSRRRLSKLPPYYQVLQLRIEGMSYLKTFQYALSLKTSLEKMGLTVLGPTSSVLLKQLENYRV
LLTIKYQGSLKTFNHLLKSNKDIKLYMNHDLSWY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=474, Percent_Identity=25.9493670886076, Blast_Score=182, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86871; Mature: 86871

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARVIVDIKHQSVNQTFDYLVHEKDQLHIQKGMRVIVPFTDNNILRLGFVYDIISASDL
CCEEEEEEECHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHH
AHKYVHEILDIEPIFNDELFLMMDKLIEDPNALIAEAFETVIPKQLLIHYEKKATLLEPK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHEECCCCCCCCCC
QLPDDLVPFFKQGVWTLLKKDEVYYNRLKTLEQKGIVELKTVLKTRNKKPLITYVRIDNR
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECC
NYHGTPKQHEVLDILSAHSQMKKADLVANSSASIVNTLIKKAVIEVFYESDKLEVANFNK
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
TTKLPVINEDLKDQIKDMMDAKHQVYVSQYQDTKLDPFFFHLIHEVISQGKQVLIMVPEN
CCCCCCCCHHHHHHHHHHHCCHHHEEHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
FMIASVKSSLEKVFREELIIGLNRGQTDKQMMLKYSAILDNETKIVVGARSSVFTSFSDL
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCC
GLIIITDSNNHSYRAHEGIYYHALEIAEIRARYHGIPLYLSASSISLDNYVKIQQKTHEL
EEEEEECCCCCCEEECCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
LNLQEEVYKSIELIDMKEELKKGNTKLVSLSLNDAILNTLDQNKKVLLILNQKGYAPFVM
HHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCHHHHHHHCCCCEEEEEEECCCCCCEEE
CRTCSHVPVDPETGIPLRFDEKQNILKSNLTKYQLEFTKTCPVCGKHTVKSVGSGIDQLI
EECCCCCCCCCCCCCCEEECHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHH
TYLHKAYPTKEILKVDSEAISNKTYHDKIEDLSQIDIIVGTQMALKSNLEDKIDLVGILM
HHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCHHHHHCCCCHHHHHHHHHH
IDQWLKLPKFDAYEATYELLSQAKFITKDKLIIQSYDPTHFVLKSIIGDGSTYYKEELSR
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHH
RRLSKLPPYYQVLQLRIEGMSYLKTFQYALSLKTSLEKMGLTVLGPTSSVLLKQLENYRV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCEE
LLTIKYQGSLKTFNHLLKSNKDIKLYMNHDLSWY
EEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MIARVIVDIKHQSVNQTFDYLVHEKDQLHIQKGMRVIVPFTDNNILRLGFVYDIISASDL
CCEEEEEEECHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHH
AHKYVHEILDIEPIFNDELFLMMDKLIEDPNALIAEAFETVIPKQLLIHYEKKATLLEPK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHEECCCCCCCCCC
QLPDDLVPFFKQGVWTLLKKDEVYYNRLKTLEQKGIVELKTVLKTRNKKPLITYVRIDNR
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECC
NYHGTPKQHEVLDILSAHSQMKKADLVANSSASIVNTLIKKAVIEVFYESDKLEVANFNK
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
TTKLPVINEDLKDQIKDMMDAKHQVYVSQYQDTKLDPFFFHLIHEVISQGKQVLIMVPEN
CCCCCCCCHHHHHHHHHHHCCHHHEEHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
FMIASVKSSLEKVFREELIIGLNRGQTDKQMMLKYSAILDNETKIVVGARSSVFTSFSDL
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCC
GLIIITDSNNHSYRAHEGIYYHALEIAEIRARYHGIPLYLSASSISLDNYVKIQQKTHEL
EEEEEECCCCCCEEECCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
LNLQEEVYKSIELIDMKEELKKGNTKLVSLSLNDAILNTLDQNKKVLLILNQKGYAPFVM
HHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCHHHHHHHCCCCEEEEEEECCCCCCEEE
CRTCSHVPVDPETGIPLRFDEKQNILKSNLTKYQLEFTKTCPVCGKHTVKSVGSGIDQLI
EECCCCCCCCCCCCCCEEECHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHH
TYLHKAYPTKEILKVDSEAISNKTYHDKIEDLSQIDIIVGTQMALKSNLEDKIDLVGILM
HHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCHHHHHCCCCHHHHHHHHHH
IDQWLKLPKFDAYEATYELLSQAKFITKDKLIIQSYDPTHFVLKSIIGDGSTYYKEELSR
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHH
RRLSKLPPYYQVLQLRIEGMSYLKTFQYALSLKTSLEKMGLTVLGPTSSVLLKQLENYRV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCEE
LLTIKYQGSLKTFNHLLKSNKDIKLYMNHDLSWY
EEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]