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The map label for this gene is fmt [H]

Identifier: 162447191

GI number: 162447191

Start: 335451

End: 336365

Strand: Direct

Name: fmt [H]

Synonym: ACL_0325

Alternate gene names: 162447191

Gene position: 335451-336365 (Clockwise)

Preceding gene: 162447190

Following gene: 162447192

Centisome position: 22.41

GC content: 33.22

Gene sequence:

>915_bases
ATGATTGTATTTATGGGTACCCCAGAGTTTTCAGTACCAATTCTAGAAATGTTGATTGAAGAAAAATATCCGGTTGGGCT
AGTTGTTACACAACCAGATAGATTTGTTGGTAGAAAAAAGATATTAACACCATCACCAGTTAAGTCGCTTGCTTTAGCAC
ATAACATTGAGGTTTTTCAACCTGAAAAATTACGTCAGGATTATCAACACATCATTGATTTAAAGCCCAGCTTAATTATT
ACAGCAAGCTATGGTCAAATACTTCCTAAAGCCCTTTTAGAAGCAATACCAGCAATTAACATTCATGGTTCATTATTACC
TAAATACCGTGGTGGTGCACCCATTCAATATGCATTATTTAATGGGGACGATAAAACCGGTATTACCTTAATGGAAATGG
TCTATAAAATGGATGCCGGTGCTATGATTAAAAAGGTAGAAGTAGACATAGAACCTCTAGATGATTACGGCACTTTATCT
AATAAGTTATCACTAGCAGGTAGAGATCTATTAAAAGAAAATCTAATGAGTGTTTTAAATAAAACGTATCAAAGCACACC
GCAAAATGAGGATGAAGTGACGTTTGCATTTACACTTAAATATAGTGATGAAGCACTTGATTTTAACAAGTCAGCATCAT
GGAATCATAACCGTATTAGAGGTTTAAGTCCTAACGTTGGTGCACATTTTAACATCAAAAACACTACGGTTAAGGTGTTT
AAATCAGCAATAAGTGATATAATAGACATTCAAAGAGGTGAAATTCGTATAGAAAATAAAAGAATATTTATTGGTACGAG
TGATAGAGCCATAGAGTTAATTGAATTACAACAATCAGGTAAAAAACAAATGAAGGTTAAAGATTATTTAAATGGGCAAA
GCCTATTTATTAACGGAGGAAAAATAGATGAGTAA

Upstream 100 bases:

>100_bases
ACCAAGGTAGTTTAAAAACGTTTAATCATTTACTAAAATCTAACAAAGACATAAAATTATATATGAACCATGATTTATCA
TGGTATTAAAGGAGTAAATT

Downstream 100 bases:

>100_bases
ACCGAAACTCTTATATAACCGAGATGAGATGTTAAAAATTAACATCGACACCTTGAAAAATAGATGTAATGTATCTGTTC
TGGATATTGCAAAGATTGCA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIVFMGTPEFSVPILEMLIEEKYPVGLVVTQPDRFVGRKKILTPSPVKSLALAHNIEVFQPEKLRQDYQHIIDLKPSLII
TASYGQILPKALLEAIPAINIHGSLLPKYRGGAPIQYALFNGDDKTGITLMEMVYKMDAGAMIKKVEVDIEPLDDYGTLS
NKLSLAGRDLLKENLMSVLNKTYQSTPQNEDEVTFAFTLKYSDEALDFNKSASWNHNRIRGLSPNVGAHFNIKNTTVKVF
KSAISDIIDIQRGEIRIENKRIFIGTSDRAIELIELQQSGKKQMKVKDYLNGQSLFINGGKIDE

Sequences:

>Translated_304_residues
MIVFMGTPEFSVPILEMLIEEKYPVGLVVTQPDRFVGRKKILTPSPVKSLALAHNIEVFQPEKLRQDYQHIIDLKPSLII
TASYGQILPKALLEAIPAINIHGSLLPKYRGGAPIQYALFNGDDKTGITLMEMVYKMDAGAMIKKVEVDIEPLDDYGTLS
NKLSLAGRDLLKENLMSVLNKTYQSTPQNEDEVTFAFTLKYSDEALDFNKSASWNHNRIRGLSPNVGAHFNIKNTTVKVF
KSAISDIIDIQRGEIRIENKRIFIGTSDRAIELIELQQSGKKQMKVKDYLNGQSLFINGGKIDE
>Mature_304_residues
MIVFMGTPEFSVPILEMLIEEKYPVGLVVTQPDRFVGRKKILTPSPVKSLALAHNIEVFQPEKLRQDYQHIIDLKPSLII
TASYGQILPKALLEAIPAINIHGSLLPKYRGGAPIQYALFNGDDKTGITLMEMVYKMDAGAMIKKVEVDIEPLDDYGTLS
NKLSLAGRDLLKENLMSVLNKTYQSTPQNEDEVTFAFTLKYSDEALDFNKSASWNHNRIRGLSPNVGAHFNIKNTTVKVF
KSAISDIIDIQRGEIRIENKRIFIGTSDRAIELIELQQSGKKQMKVKDYLNGQSLFINGGKIDE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=172, Percent_Identity=26.1627906976744, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789683, Length=308, Percent_Identity=37.987012987013, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788589, Length=269, Percent_Identity=31.5985130111524, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319458, Length=174, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571984, Length=230, Percent_Identity=25.6521739130435, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550868, Length=232, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34113; Mature: 34113

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVFMGTPEFSVPILEMLIEEKYPVGLVVTQPDRFVGRKKILTPSPVKSLALAHNIEVFQ
CEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHCCCEEC
PEKLRQDYQHIIDLKPSLIITASYGQILPKALLEAIPAINIHGSLLPKYRGGAPIQYALF
HHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEE
NGDDKTGITLMEMVYKMDAGAMIKKVEVDIEPLDDYGTLSNKLSLAGRDLLKENLMSVLN
CCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KTYQSTPQNEDEVTFAFTLKYSDEALDFNKSASWNHNRIRGLSPNVGAHFNIKNTTVKVF
HHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCEEEEECHHHHHHH
KSAISDIIDIQRGEIRIENKRIFIGTSDRAIELIELQQSGKKQMKVKDYLNGQSLFINGG
HHHHHHHHHCCCCEEEEECCEEEEECCCCEEEEEEEHHCCCHHEEHHHHCCCCEEEEECC
KIDE
CCCC
>Mature Secondary Structure
MIVFMGTPEFSVPILEMLIEEKYPVGLVVTQPDRFVGRKKILTPSPVKSLALAHNIEVFQ
CEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHCCCEEC
PEKLRQDYQHIIDLKPSLIITASYGQILPKALLEAIPAINIHGSLLPKYRGGAPIQYALF
HHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEE
NGDDKTGITLMEMVYKMDAGAMIKKVEVDIEPLDDYGTLSNKLSLAGRDLLKENLMSVLN
CCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KTYQSTPQNEDEVTFAFTLKYSDEALDFNKSASWNHNRIRGLSPNVGAHFNIKNTTVKVF
HHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCEEEEECHHHHHHH
KSAISDIIDIQRGEIRIENKRIFIGTSDRAIELIELQQSGKKQMKVKDYLNGQSLFINGG
HHHHHHHHHCCCCEEEEECCEEEEECCCCEEEEEEEHHCCCHHEEHHHHCCCCEEEEECC
KIDE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA