Click here to switch to the map view.

The map label for this gene is papS [H]

Identifier: 162447078

GI number: 162447078

Start: 198838

End: 199986

Strand: Direct

Name: papS [H]

Synonym: ACL_0208

Alternate gene names: 162447078

Gene position: 198838-199986 (Clockwise)

Preceding gene: 162447076

Following gene: 162447079

Centisome position: 13.28

GC content: 33.25

Gene sequence:

>1149_bases
ATGAGTTTAAAATCAGCGAAAAATTTAATCAGAGATTTAAAGAAACATGGCTATCAAGCTTACTTAGTTGGAGGCGTGGT
CAGAGACTTTCTAATGAAAGCCAAGTTTAGTGATATCGATATAACTACCAGTGCCAAACCATATGAAGTTATGAAACATT
TTAAGGCGGTCCCTACAGGTATTAAATATGGTACGATAACTATCATGTATGATAATGTATCCTTTGAAGTGACAACCTTT
AGAACAGATGGTCCTACATCTGACTTTAGACATCCGGATTCCGTCATTTACTCAGATAACGTTAATGATGATGTGATGCG
TCGTGATTTTACAATGAATGGTATCTTAATGGATGAGTATCAAAATATCTATGACTACGTAGGTGGTCAAAGAGATATCG
AACATAAAATCATCCGTGCGATTGGAGATCCAAACGAACGCTTTAAAGAAGATGCACTACGTATGTTACGAGCATTCTAT
TTCCAATCCAAATTAGGCTTTGAAATAGAACCTGTAACCAAACAAGCAATTCAAGATAATGCACATTTAATTAAAGAGTT
AGCGATGGAACGCATTCATGCTGAACTCATTAAAATACTTAAAGGTAAACATCTAAAAACGGCCTTAAACTCCATGATTG
AAACAGGTATACATAAAGTACTACCTGGATTAGAAAAAGGGATTATACATACCGCTAATTTAGAAGAAATGCCTTTTATT
GATACATTCTTTGCTTTATCATTTACTTTAAACAATGGATTTATTCCGGCTAAATGGACATTTTCAAATAGACACCGTCA
TAAATATCAAAAGGCAAGTGAACTTGCTTTAATGCACCCAACAACAATTCCAGATACTGCACTGTATCACTATGGATTAG
AGATATCTTTACTTGCTAATAAAGTGAACTTTAACTTAGGTAAACAAAGTTATCAAAATCAATCACTTCAAGAAAGATTT
AATAACTTACCACTAAAAAGTGAGTTGGATCTTGCACTGACAAGTCAAGAGATTATGAATATTGTAAATAAAAAAGCCGG
TGCTTGGTTAGGTAAATTAAAAGCACACATGATTGATCATATTCTAGAAAAGAAACTGTCTAACAATAAAGAAGCATTAA
CAAGTTTTGTTAGTACATATGAGGTGTAA

Upstream 100 bases:

>100_bases
TATAAGTTTTTCACACATTTATTGAGTCCTCCCTTTTTATAATGTGCTATTTTTGATATAATGTTAAAGGTATATAAAAT
AATTGGTAGGTTATAAAAAT

Downstream 100 bases:

>100_bases
CAGATGGACATAATTATTGGTAAAGTAGAATCTATTAATAAAGGTGACAACTATTGTAATGCATCTTTATCCTTAGAAGA
TAAAAAACATTTCAATGTGA

Product: poly(A) polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSLKSAKNLIRDLKKHGYQAYLVGGVVRDFLMKAKFSDIDITTSAKPYEVMKHFKAVPTGIKYGTITIMYDNVSFEVTTF
RTDGPTSDFRHPDSVIYSDNVNDDVMRRDFTMNGILMDEYQNIYDYVGGQRDIEHKIIRAIGDPNERFKEDALRMLRAFY
FQSKLGFEIEPVTKQAIQDNAHLIKELAMERIHAELIKILKGKHLKTALNSMIETGIHKVLPGLEKGIIHTANLEEMPFI
DTFFALSFTLNNGFIPAKWTFSNRHRHKYQKASELALMHPTTIPDTALYHYGLEISLLANKVNFNLGKQSYQNQSLQERF
NNLPLKSELDLALTSQEIMNIVNKKAGAWLGKLKAHMIDHILEKKLSNNKEALTSFVSTYEV

Sequences:

>Translated_382_residues
MSLKSAKNLIRDLKKHGYQAYLVGGVVRDFLMKAKFSDIDITTSAKPYEVMKHFKAVPTGIKYGTITIMYDNVSFEVTTF
RTDGPTSDFRHPDSVIYSDNVNDDVMRRDFTMNGILMDEYQNIYDYVGGQRDIEHKIIRAIGDPNERFKEDALRMLRAFY
FQSKLGFEIEPVTKQAIQDNAHLIKELAMERIHAELIKILKGKHLKTALNSMIETGIHKVLPGLEKGIIHTANLEEMPFI
DTFFALSFTLNNGFIPAKWTFSNRHRHKYQKASELALMHPTTIPDTALYHYGLEISLLANKVNFNLGKQSYQNQSLQERF
NNLPLKSELDLALTSQEIMNIVNKKAGAWLGKLKAHMIDHILEKKLSNNKEALTSFVSTYEV
>Mature_381_residues
SLKSAKNLIRDLKKHGYQAYLVGGVVRDFLMKAKFSDIDITTSAKPYEVMKHFKAVPTGIKYGTITIMYDNVSFEVTTFR
TDGPTSDFRHPDSVIYSDNVNDDVMRRDFTMNGILMDEYQNIYDYVGGQRDIEHKIIRAIGDPNERFKEDALRMLRAFYF
QSKLGFEIEPVTKQAIQDNAHLIKELAMERIHAELIKILKGKHLKTALNSMIETGIHKVLPGLEKGIIHTANLEEMPFID
TFFALSFTLNNGFIPAKWTFSNRHRHKYQKASELALMHPTTIPDTALYHYGLEISLLANKVNFNLGKQSYQNQSLQERFN
NLPLKSELDLALTSQEIMNIVNKKAGAWLGKLKAHMIDHILEKKLSNNKEALTSFVSTYEV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=239, Percent_Identity=32.2175732217573, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI87081691, Length=204, Percent_Identity=37.2549019607843, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71995920, Length=237, Percent_Identity=36.2869198312236, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281360112, Length=255, Percent_Identity=31.7647058823529, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357337, Length=255, Percent_Identity=31.7647058823529, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 43771; Mature: 43640

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKSAKNLIRDLKKHGYQAYLVGGVVRDFLMKAKFSDIDITTSAKPYEVMKHFKAVPTG
CCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCCCC
IKYGTITIMYDNVSFEVTTFRTDGPTSDFRHPDSVIYSDNVNDDVMRRDFTMNGILMDEY
CEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHCCCCCCEEHHHH
QNIYDYVGGQRDIEHKIIRAIGDPNERFKEDALRMLRAFYFQSKLGFEIEPVTKQAIQDN
HHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHH
AHLIKELAMERIHAELIKILKGKHLKTALNSMIETGIHKVLPGLEKGIIHTANLEEMPFI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEECCCCCCCCH
DTFFALSFTLNNGFIPAKWTFSNRHRHKYQKASELALMHPTTIPDTALYHYGLEISLLAN
HHHHHHEEEECCCEEEEEEECCCCHHHHHHHHHHEEEECCCCCCCHHHHHCCCEEEEEEE
KVNFNLGKQSYQNQSLQERFNNLPLKSELDLALTSQEIMNIVNKKAGAWLGKLKAHMIDH
HHCCCCCHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEKKLSNNKEALTSFVSTYEV
HHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SLKSAKNLIRDLKKHGYQAYLVGGVVRDFLMKAKFSDIDITTSAKPYEVMKHFKAVPTG
CHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCCCC
IKYGTITIMYDNVSFEVTTFRTDGPTSDFRHPDSVIYSDNVNDDVMRRDFTMNGILMDEY
CEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHCCCCCCEEHHHH
QNIYDYVGGQRDIEHKIIRAIGDPNERFKEDALRMLRAFYFQSKLGFEIEPVTKQAIQDN
HHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHH
AHLIKELAMERIHAELIKILKGKHLKTALNSMIETGIHKVLPGLEKGIIHTANLEEMPFI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEECCCCCCCCH
DTFFALSFTLNNGFIPAKWTFSNRHRHKYQKASELALMHPTTIPDTALYHYGLEISLLAN
HHHHHHEEEECCCEEEEEEECCCCHHHHHHHHHHEEEECCCCCCCHHHHHCCCEEEEEEE
KVNFNLGKQSYQNQSLQERFNNLPLKSELDLALTSQEIMNIVNKKAGAWLGKLKAHMIDH
HHCCCCCHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEKKLSNNKEALTSFVSTYEV
HHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA