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The map label for this gene is aspS

Identifier: 162319786

GI number: 162319786

Start: 3031963

End: 3033759

Strand: Reverse

Name: aspS

Synonym: amb2837

Alternate gene names: 162319786

Gene position: 3033759-3031963 (Counterclockwise)

Preceding gene: 83311937

Following gene: 83311934

Centisome position: 61.08

GC content: 65.39

Gene sequence:

>1797_bases
ATGCACGTCTATCGGTCACATACCTGCGGTCAGTTGAAGGCGGCGGATGCCGGCATCCAGGCTCGCCTCTCGGGCTGGGT
TCATCGCAAGCGCGACCACGGCAATCTGCTGTTCGTCGACCTGCGCGACCATTACGGCATCACCCAATGCGTGATCGACG
TGTCCAGCCCGGTCTTCGCCGCCCTGGACAAGGCCCGGCCGGAAAGCGTCATCACCGTCACCGGCAAGGTGGTCAAGCGC
TCGGCCGAGACCATCAATCCCCGCCTGCCCACCGGCGAGATCGAGCTGCAGGTGGCCGAGGTGGAGATTCAGTCCATCGC
CGACGTGCTGCCCATCCAGGTGGCCGGCGACCAGGAATATCCCGAGGACATGCGCCTGCGGTACCGCTTCCTCGACCTGC
GCCGCGAGGACGTGCACGCCAATATGATGCTGCGTTCGCGCGTCATCGCCTATCTGCGTCAGGCCATGATCGGCCAGGGC
TTCACCGAGTTCCAGACCCCGATCCTGACCGCCAGCAGCCCCGAGGGTGCCCGCGACTATCTGGTGCCGTCGCGCATCCA
TCCTGGCAAGTTCTACGCCCTGCCCCAGGCGCCGCAGCAGTTCAAGCAGCTGCTGATGGTCGCCGGCTTCGACAAGTACT
TCCAGATCGCGCCGTGCTTCCGTGACGAAGCCGGCCGCGCCGACCGGTCCCCGGGCGAGTTCTACCAGCTCGATTTCGAA
ATGAGCTACGTCACCCAGGACGACGTCTTCGCCGCCATCGAGCCGGTGCTGGAAGGCGTGTTCAAGGAATTCGGCAAGGG
CCGCGCCGTCACTCCGGCGCCGTTCCCCCGCATCACCTATGCGGATTCCATGCTGAAATACGGCTCGGACAAGCCCGACC
TGCGCAACCCCATCATCATCGCCGACGTCACCGAGCCCTTCCGCGGCTCGGGCTTCGGCCTGTTCGCCAAGCTGGTGGAC
AAGGGCGCCGTGGTGCGCGCCATTCCGGCCCCGGGGGCGGCGGGCCAGCCGCGCTCGTGGTTCGACAAGCTCAACGACTG
GGCGCGTGAGAACGGCGCCGGCGGTCTGGGCTACATCCAGTTCGCCGCCGATGGCCCCAAGGGTCCCATCGCCAAGAACC
TGGAGCCCGCCCGGGTCGAGGCCATCAAGGCCGCCGCCAACCTCAAGGACGGCGACGCGGTGTTCTTCGCCTGCGACAAG
GCGCTGCCGGCGGCCAAGTTCGCCGGCCTGGTCCGCACCAAGATCGGCAACGAGTTGGATCTGCTGGAAAAGGACGTCTT
CAAGTTCTGCTGGACCGTTGATTTCCCCATGTACGAGATCAACGAGGAGACGGGCCTTGTGGAGTTCTCCCACAATCCGT
TCTCCATGCCCCAGGGCGGCATGGATGCGCTGCTCAACCAGGACCCGCTGACCATCAACGCCTATCAGTATGACATCGTC
TGCAACGGCGTCGAACTGTCGTCGGGCGCCATCCGGAACCACCGCCCGGACATCATGTACAAGGCCTTCGAGATCGCCGG
CTATTCCGCCGCCCATGTGGAGGAGCATTTCGGCGGCATGCTCAACGCCTTCAAGTTCGGCGCCCCGCCCCACGGCGGCT
CGGCCCCCGGCGTCGACCGCATCGTCATGCTGCTGGCCGACCAGCCCAACATCCGCGAGATCATCCTGTTCCCCATGAAC
CAGCAGGCCCAGGACCTGCTGATGCAGGCCCCCGCCGAAATCGCCATGGAGCGCCTGCGCGAACTCCACATCAAGGTGGA
CCTGCCCAAGCCGAAGAAAGAGGTCAAGGAGGGCTGA

Upstream 100 bases:

>100_bases
TGTGGCCGGACTCGTAAATATTGGGTTATCCCGCCTTGACAAAATATGGCCCGGGATGCCTTCTTCCCGCTTTGGATTCA
AGCGCGCGTACGAGGGTTTC

Downstream 100 bases:

>100_bases
GGCCCTTCTATCCGTCGAGACGAGGAAGGGGGCCATTGGCCCCCTTTTTCATGGGCGCGGCCGCTGACACGCCACTCCGA
GTTTCCGTCTTTCGGCCGGG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MHVYRSHTCGQLKAADAGIQARLSGWVHRKRDHGNLLFVDLRDHYGITQCVIDVSSPVFAALDKARPESVITVTGKVVKR
SAETINPRLPTGEIELQVAEVEIQSIADVLPIQVAGDQEYPEDMRLRYRFLDLRREDVHANMMLRSRVIAYLRQAMIGQG
FTEFQTPILTASSPEGARDYLVPSRIHPGKFYALPQAPQQFKQLLMVAGFDKYFQIAPCFRDEAGRADRSPGEFYQLDFE
MSYVTQDDVFAAIEPVLEGVFKEFGKGRAVTPAPFPRITYADSMLKYGSDKPDLRNPIIIADVTEPFRGSGFGLFAKLVD
KGAVVRAIPAPGAAGQPRSWFDKLNDWARENGAGGLGYIQFAADGPKGPIAKNLEPARVEAIKAAANLKDGDAVFFACDK
ALPAAKFAGLVRTKIGNELDLLEKDVFKFCWTVDFPMYEINEETGLVEFSHNPFSMPQGGMDALLNQDPLTINAYQYDIV
CNGVELSSGAIRNHRPDIMYKAFEIAGYSAAHVEEHFGGMLNAFKFGAPPHGGSAPGVDRIVMLLADQPNIREIILFPMN
QQAQDLLMQAPAEIAMERLRELHIKVDLPKPKKEVKEG

Sequences:

>Translated_598_residues
MHVYRSHTCGQLKAADAGIQARLSGWVHRKRDHGNLLFVDLRDHYGITQCVIDVSSPVFAALDKARPESVITVTGKVVKR
SAETINPRLPTGEIELQVAEVEIQSIADVLPIQVAGDQEYPEDMRLRYRFLDLRREDVHANMMLRSRVIAYLRQAMIGQG
FTEFQTPILTASSPEGARDYLVPSRIHPGKFYALPQAPQQFKQLLMVAGFDKYFQIAPCFRDEAGRADRSPGEFYQLDFE
MSYVTQDDVFAAIEPVLEGVFKEFGKGRAVTPAPFPRITYADSMLKYGSDKPDLRNPIIIADVTEPFRGSGFGLFAKLVD
KGAVVRAIPAPGAAGQPRSWFDKLNDWARENGAGGLGYIQFAADGPKGPIAKNLEPARVEAIKAAANLKDGDAVFFACDK
ALPAAKFAGLVRTKIGNELDLLEKDVFKFCWTVDFPMYEINEETGLVEFSHNPFSMPQGGMDALLNQDPLTINAYQYDIV
CNGVELSSGAIRNHRPDIMYKAFEIAGYSAAHVEEHFGGMLNAFKFGAPPHGGSAPGVDRIVMLLADQPNIREIILFPMN
QQAQDLLMQAPAEIAMERLRELHIKVDLPKPKKEVKEG
>Mature_598_residues
MHVYRSHTCGQLKAADAGIQARLSGWVHRKRDHGNLLFVDLRDHYGITQCVIDVSSPVFAALDKARPESVITVTGKVVKR
SAETINPRLPTGEIELQVAEVEIQSIADVLPIQVAGDQEYPEDMRLRYRFLDLRREDVHANMMLRSRVIAYLRQAMIGQG
FTEFQTPILTASSPEGARDYLVPSRIHPGKFYALPQAPQQFKQLLMVAGFDKYFQIAPCFRDEAGRADRSPGEFYQLDFE
MSYVTQDDVFAAIEPVLEGVFKEFGKGRAVTPAPFPRITYADSMLKYGSDKPDLRNPIIIADVTEPFRGSGFGLFAKLVD
KGAVVRAIPAPGAAGQPRSWFDKLNDWARENGAGGLGYIQFAADGPKGPIAKNLEPARVEAIKAAANLKDGDAVFFACDK
ALPAAKFAGLVRTKIGNELDLLEKDVFKFCWTVDFPMYEINEETGLVEFSHNPFSMPQGGMDALLNQDPLTINAYQYDIV
CNGVELSSGAIRNHRPDIMYKAFEIAGYSAAHVEEHFGGMLNAFKFGAPPHGGSAPGVDRIVMLLADQPNIREIILFPMN
QQAQDLLMQAPAEIAMERLRELHIKVDLPKPKKEVKEG

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=34.8184818481848, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI45439306, Length=269, Percent_Identity=28.2527881040892, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI5031815, Length=266, Percent_Identity=27.4436090225564, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI194272210, Length=266, Percent_Identity=27.4436090225564, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI226491502, Length=258, Percent_Identity=23.2558139534884, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=40.2376910016978, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1789256, Length=320, Percent_Identity=26.5625, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790571, Length=317, Percent_Identity=26.1829652996845, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=596, Percent_Identity=33.5570469798658, Blast_Score=286, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=24.188790560472, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=330, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=24.188790560472, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=25.1798561151079, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=649, Percent_Identity=30.6625577812018, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321807, Length=290, Percent_Identity=24.4827586206897, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=275, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24584738, Length=605, Percent_Identity=34.7107438016529, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI17136276, Length=260, Percent_Identity=28.8461538461538, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640849, Length=253, Percent_Identity=25.296442687747, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640851, Length=253, Percent_Identity=25.296442687747, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921528, Length=290, Percent_Identity=25.5172413793103, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_MAGSA (Q2W3D5)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422199.2
- ProteinModelPortal:   Q2W3D5
- SMR:   Q2W3D5
- STRING:   Q2W3D5
- GeneID:   3802624
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2837
- NMPDR:   fig|342108.5.peg.2520
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   MMAG342108:AMB2837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66303; Mature: 66303

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVYRSHTCGQLKAADAGIQARLSGWVHRKRDHGNLLFVDLRDHYGITQCVIDVSSPVFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCHHHH
ALDKARPESVITVTGKVVKRSAETINPRLPTGEIELQVAEVEIQSIADVLPIQVAGDQEY
HHHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHHHHHCCEEEECCCCC
PEDMRLRYRFLDLRREDVHANMMLRSRVIAYLRQAMIGQGFTEFQTPILTASSPEGARDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCC
LVPSRIHPGKFYALPQAPQQFKQLLMVAGFDKYFQIAPCFRDEAGRADRSPGEFYQLDFE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCHHCCCCCCCCCCCCEEEEEEE
MSYVTQDDVFAAIEPVLEGVFKEFGKGRAVTPAPFPRITYADSMLKYGSDKPDLRNPIII
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEE
ADVTEPFRGSGFGLFAKLVDKGAVVRAIPAPGAAGQPRSWFDKLNDWARENGAGGLGYIQ
EECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
FAADGPKGPIAKNLEPARVEAIKAAANLKDGDAVFFACDKALPAAKFAGLVRTKIGNELD
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHH
LLEKDVFKFCWTVDFPMYEINEETGLVEFSHNPFSMPQGGMDALLNQDPLTINAYQYDIV
HHHHHHHHHHHEECCCCEEECCCCCEEEECCCCCCCCCCCHHHHCCCCCCEEEEEEEEEE
CNGVELSSGAIRNHRPDIMYKAFEIAGYSAAHVEEHFGGMLNAFKFGAPPHGGSAPGVDR
ECCEEECCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADQPNIREIILFPMNQQAQDLLMQAPAEIAMERLRELHIKVDLPKPKKEVKEG
EEEEECCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCHHHHCCC
>Mature Secondary Structure
MHVYRSHTCGQLKAADAGIQARLSGWVHRKRDHGNLLFVDLRDHYGITQCVIDVSSPVFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCHHHH
ALDKARPESVITVTGKVVKRSAETINPRLPTGEIELQVAEVEIQSIADVLPIQVAGDQEY
HHHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHHHHHCCEEEECCCCC
PEDMRLRYRFLDLRREDVHANMMLRSRVIAYLRQAMIGQGFTEFQTPILTASSPEGARDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCC
LVPSRIHPGKFYALPQAPQQFKQLLMVAGFDKYFQIAPCFRDEAGRADRSPGEFYQLDFE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCHHCCCCCCCCCCCCEEEEEEE
MSYVTQDDVFAAIEPVLEGVFKEFGKGRAVTPAPFPRITYADSMLKYGSDKPDLRNPIII
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEE
ADVTEPFRGSGFGLFAKLVDKGAVVRAIPAPGAAGQPRSWFDKLNDWARENGAGGLGYIQ
EECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
FAADGPKGPIAKNLEPARVEAIKAAANLKDGDAVFFACDKALPAAKFAGLVRTKIGNELD
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHH
LLEKDVFKFCWTVDFPMYEINEETGLVEFSHNPFSMPQGGMDALLNQDPLTINAYQYDIV
HHHHHHHHHHHEECCCCEEECCCCCEEEECCCCCCCCCCCHHHHCCCCCCEEEEEEEEEE
CNGVELSSGAIRNHRPDIMYKAFEIAGYSAAHVEEHFGGMLNAFKFGAPPHGGSAPGVDR
ECCEEECCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADQPNIREIILFPMNQQAQDLLMQAPAEIAMERLRELHIKVDLPKPKKEVKEG
EEEEECCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA