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The map label for this gene is murF [H]

Identifier: 162148957

GI number: 162148957

Start: 3273863

End: 3275272

Strand: Direct

Name: murF [H]

Synonym: GDI_3187

Alternate gene names: 162148957

Gene position: 3273863-3275272 (Clockwise)

Preceding gene: 162148956

Following gene: 162148958

Centisome position: 83.01

GC content: 72.2

Gene sequence:

>1410_bases
ATGACGGTTCTGTGGACCCGCGCGGCACTGCAGGACGCGACCGGCGGCACGTTCACGGGGCAGGCGCCGGTTGCAGTCAC
CGGGGTGTCGATCGACACCCGCACGCTGGCCCCGGGCGACCTGTTCATCGCGCTGGCGGGCGAAAACAGCGATGGCCATG
CCCACGTCCGCGCGGCCCTGGACCAGGGCGCCGCCGCCGCCATGATCCATGACCCGACTGTCCTGGGCACCGATTCCGAC
GATCCGCGCCTGCTGCGCGTGCCCGACACGATGGCGGCGCTGAACGCGCTGGGGCGCTATGCCCGCACCCGCTTCACCGG
CCGGATGGTCGCCGTCACCGGCAGCGTGGGCAAGACCACGACCAAGGAGATGCTGCGCCTGGCGCTGGGCGCGATCGGGC
CGACCCATTGCGCCGCCGCGTCCTACAACAACCATTGGGGCGTACCGCTGACGCTGGCCCGCCTGCCGATGGATGCCGCC
TTCTGCGTCTGCGAGGTGGGCATGAACCACCCCGGAGAAATCGCGCCGCTGGCGGTGATGGTCCGGCCGCTTGTCGCCCT
CGTCACCACCATCGCCGGCGCCCATCTGGGCCATATGGGCAGCCTGGAGGCCATCGCGAGGGAAAAATCCGACCTGATCG
CGACCCTGCCGCCCGCCGGCATCGCCATTGTCCCCGATGACGCCACGGGCCAGCCGATCTTCGCCGAACAGGCGGCGCGG
GCACGGGCCACATTGTGGCGGGCCGGCGAACGGACCGACAGCACGGTGGCCGTGTCGGACGTGGCGCTGGATGCCGAGGG
CAGCAGCTTCACCGTCACCATCGACGGACGGTCCGTCCCCGTACGCCTGAACGCCCCGGGCCGCCACCTGGTCCGCAACG
CCGCCACGGCGCTGGCGGCGGTGGCCGCCCTGGGCGCCGACATCGCGACCGCCGCCACGGCGCTGGCGGATTTCCGGCCC
GGCGCCGGGCGCGGCGCGCTGAAGCCGATCCTGGGCGGCCGCGCCGCCCTGCTGGATGAAAGCTACAACGCCTCGGTCCT
GTCCGTCCGCTCGTCCCTGGCGGTGCTGGGGCTGCTGCCCGCGACACGGCGCATGGCCGTGCTGGGCGATATCCGCGAAC
TCGGCGCCTTCGCGCGCGCCGAACATGAATCCCTCCTGTCCGCCGTGCTGGCGCATGCGGACCTCGTCTTCTGCTCCGGC
CCCGATATGAAATATCTGTTCGACCTGCTTCCCCCCTCCCGACGCGGCGCATGGGCGCCGGACGCCGCCAGCCTGGCGCC
GCTGGTGCGCGATGCCGTGGCCCAGGACGACGTGATCCTGGTCAAGGGCAGCTTCGGCAGCCGCATGCGCGATGTCGTCT
CGGTGCTGACCGCGCCCGCGACGGCACCCTCCAAGGCGGGTGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGTGGCCGGCAAGGGCCACGAACAGGGCCAGACCATCGGCACCGCGACGCTGCCCTTCGATGACGCATCGGTCATTCGC
GACCTGGCCGGGGTGCGGGC

Downstream 100 bases:

>100_bases
TGCTCTACGACCTGATCCAGCATCACGGGTCGGCACACGTCACCGTCCTGAACCTGTTCCGCTACATCACCTTCCGCGCC
GGGGCGGCGTGCCTCACGGC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTVLWTRAALQDATGGTFTGQAPVAVTGVSIDTRTLAPGDLFIALAGENSDGHAHVRAALDQGAAAAMIHDPTVLGTDSD
DPRLLRVPDTMAALNALGRYARTRFTGRMVAVTGSVGKTTTKEMLRLALGAIGPTHCAAASYNNHWGVPLTLARLPMDAA
FCVCEVGMNHPGEIAPLAVMVRPLVALVTTIAGAHLGHMGSLEAIAREKSDLIATLPPAGIAIVPDDATGQPIFAEQAAR
ARATLWRAGERTDSTVAVSDVALDAEGSSFTVTIDGRSVPVRLNAPGRHLVRNAATALAAVAALGADIATAATALADFRP
GAGRGALKPILGGRAALLDESYNASVLSVRSSLAVLGLLPATRRMAVLGDIRELGAFARAEHESLLSAVLAHADLVFCSG
PDMKYLFDLLPPSRRGAWAPDAASLAPLVRDAVAQDDVILVKGSFGSRMRDVVSVLTAPATAPSKAGAA

Sequences:

>Translated_469_residues
MTVLWTRAALQDATGGTFTGQAPVAVTGVSIDTRTLAPGDLFIALAGENSDGHAHVRAALDQGAAAAMIHDPTVLGTDSD
DPRLLRVPDTMAALNALGRYARTRFTGRMVAVTGSVGKTTTKEMLRLALGAIGPTHCAAASYNNHWGVPLTLARLPMDAA
FCVCEVGMNHPGEIAPLAVMVRPLVALVTTIAGAHLGHMGSLEAIAREKSDLIATLPPAGIAIVPDDATGQPIFAEQAAR
ARATLWRAGERTDSTVAVSDVALDAEGSSFTVTIDGRSVPVRLNAPGRHLVRNAATALAAVAALGADIATAATALADFRP
GAGRGALKPILGGRAALLDESYNASVLSVRSSLAVLGLLPATRRMAVLGDIRELGAFARAEHESLLSAVLAHADLVFCSG
PDMKYLFDLLPPSRRGAWAPDAASLAPLVRDAVAQDDVILVKGSFGSRMRDVVSVLTAPATAPSKAGAA
>Mature_468_residues
TVLWTRAALQDATGGTFTGQAPVAVTGVSIDTRTLAPGDLFIALAGENSDGHAHVRAALDQGAAAAMIHDPTVLGTDSDD
PRLLRVPDTMAALNALGRYARTRFTGRMVAVTGSVGKTTTKEMLRLALGAIGPTHCAAASYNNHWGVPLTLARLPMDAAF
CVCEVGMNHPGEIAPLAVMVRPLVALVTTIAGAHLGHMGSLEAIAREKSDLIATLPPAGIAIVPDDATGQPIFAEQAARA
RATLWRAGERTDSTVAVSDVALDAEGSSFTVTIDGRSVPVRLNAPGRHLVRNAATALAAVAALGADIATAATALADFRPG
AGRGALKPILGGRAALLDESYNASVLSVRSSLAVLGLLPATRRMAVLGDIRELGAFARAEHESLLSAVLAHADLVFCSGP
DMKYLFDLLPPSRRGAWAPDAASLAPLVRDAVAQDDVILVKGSFGSRMRDVVSVLTAPATAPSKAGAA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=462, Percent_Identity=35.7142857142857, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48214; Mature: 48083

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLWTRAALQDATGGTFTGQAPVAVTGVSIDTRTLAPGDLFIALAGENSDGHAHVRAAL
CEEEEEHHHHHCCCCCEECCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHH
DQGAAAAMIHDPTVLGTDSDDPRLLRVPDTMAALNALGRYARTRFTGRMVAVTGSVGKTT
HCCCEEEEEECCEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
TKEMLRLALGAIGPTHCAAASYNNHWGVPLTLARLPMDAAFCVCEVGMNHPGEIAPLAVM
HHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHEECCCCCCCCHHHHHHH
VRPLVALVTTIAGAHLGHMGSLEAIAREKSDLIATLPPAGIAIVPDDATGQPIFAEQAAR
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHH
ARATLWRAGERTDSTVAVSDVALDAEGSSFTVTIDGRSVPVRLNAPGRHLVRNAATALAA
HHHHHHHCCCCCCCEEEEEEEEEECCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHH
VAALGADIATAATALADFRPGAGRGALKPILGGRAALLDESYNASVLSVRSSLAVLGLLP
HHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCC
ATRRMAVLGDIRELGAFARAEHESLLSAVLAHADLVFCSGPDMKYLFDLLPPSRRGAWAP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCCC
DAASLAPLVRDAVAQDDVILVKGSFGSRMRDVVSVLTAPATAPSKAGAA
CHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TVLWTRAALQDATGGTFTGQAPVAVTGVSIDTRTLAPGDLFIALAGENSDGHAHVRAAL
EEEEEHHHHHCCCCCEECCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHH
DQGAAAAMIHDPTVLGTDSDDPRLLRVPDTMAALNALGRYARTRFTGRMVAVTGSVGKTT
HCCCEEEEEECCEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
TKEMLRLALGAIGPTHCAAASYNNHWGVPLTLARLPMDAAFCVCEVGMNHPGEIAPLAVM
HHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHEECCCCCCCCHHHHHHH
VRPLVALVTTIAGAHLGHMGSLEAIAREKSDLIATLPPAGIAIVPDDATGQPIFAEQAAR
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHH
ARATLWRAGERTDSTVAVSDVALDAEGSSFTVTIDGRSVPVRLNAPGRHLVRNAATALAA
HHHHHHHCCCCCCCEEEEEEEEEECCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHH
VAALGADIATAATALADFRPGAGRGALKPILGGRAALLDESYNASVLSVRSSLAVLGLLP
HHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCC
ATRRMAVLGDIRELGAFARAEHESLLSAVLAHADLVFCSGPDMKYLFDLLPPSRRGAWAP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCCC
DAASLAPLVRDAVAQDDVILVKGSFGSRMRDVVSVLTAPATAPSKAGAA
CHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]