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The map label for this gene is nemA [H]

Identifier: 162148549

GI number: 162148549

Start: 2859436

End: 2860530

Strand: Reverse

Name: nemA [H]

Synonym: GDI_2773

Alternate gene names: 162148549

Gene position: 2860530-2859436 (Counterclockwise)

Preceding gene: 162148550

Following gene: 162148548

Centisome position: 72.53

GC content: 66.3

Gene sequence:

>1095_bases
ATGTCCATGCTTTTCACGCCTTTCAGGGCAGGCGCGCTGTCGCTCGGTCATCGGGTCGTCATGGCGCCGCTCACCCGCAT
GCGTTCGGAACCCGGCGACAAGGCCGGTGCGCTCATGCGCGAATATTACGCCCAACGCACATCGCATGGCGGCCTCATCG
TGTCGGAAGCCACGCCAGTCGCGCGGGAAGGATACGGTTATGCCGGAGCACCCGGCATCTACGACGATGCGCAGATCGAG
GGCTGGCGCACGGTGACCGACGCCGTGCATGCCAGCGGCGGCAAGATCGTCATGCAGCTCTGGCACGTCGGACGGCAGTC
CCACCGCGATCTTCAGCCGGACGGCGGCGCACCCGTGGCACCCTCCGCCATCCAGGCGGAAGGCGATGCCTACACGCCGA
ACGGGCCGGCGCCGTTCTCGATGCCGCGCGCGCTGGCGCTTCATGAAATTCCCGGCGTGATCGAGCAGTTCCGCCAGGGC
GCGGCGCGTGCGAAAGCGGCCGGTTTCGACGGTGTGGAAATCCACGGTGCGAATGGTTACCTGCCCGACCAGTTTTTGCA
GGACGGCACCAACCACCGCACTGACGCGTATGGCGGCCCGATCGAAAACCGCGCGCGCTTCCTGCTGGAAGTGACGCAGG
CGGCCATCGATGTGTGGGGCGCGGATCGCGTCGGCGTGCGGCTGAGCCCAAGCGGCACTTACGGTTCGATGTCCGACAGC
AACCCGCAGGAGACCTTCGGCTATGTCGCGGAGAAGCTGGACGAAATGGGCATCGCCTATCTCCATGTCGTCGAGCCGCG
CATCAAGGGCACGGAACTGGTCGCCGAGAGCGATGCCGTCGCGGCCCGGCATTTGCGCGGCGCCTTCAGCGGCACGCTCA
TTGCCGCCGGCGGTTTTGATGGCGCCAGTGCCGAGGCGATCATCCGGGCAGGCGACGCCGATGCCGTCGCTTTCGGGCGG
GCGTTCATCAGCAATCCCGATCTGCCGGAGCGTCTGCGACGCAATCTGCCGCTGAACCCGTATGACCGTTCGACCTTCTA
CGGCGGCGATGCGCATGGCTACACCGATTATCCGGCGCTTGAATCCGTCGGGTGA

Upstream 100 bases:

>100_bases
TACGAGACGGTCGAAGCACGCCCGGCCGTGCAACGCGGCATCGCCCGCGTCGAAGCGCTCGTCCCGCAAGACTGACGTCA
TCCCTTTCAAGGAAAACATC

Downstream 100 bases:

>100_bases
GATCTCATTCCGGCCCGGAATGACCAATAGTCGTCCGAGCCGTCATCCCCCATGCAGCAGACCGCTCTATCTCAAGCATG
TCGCTACGACTGACAAAGGA

Product: N-ethylmaleimide reductase, FMN-linked

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSMLFTPFRAGALSLGHRVVMAPLTRMRSEPGDKAGALMREYYAQRTSHGGLIVSEATPVAREGYGYAGAPGIYDDAQIE
GWRTVTDAVHASGGKIVMQLWHVGRQSHRDLQPDGGAPVAPSAIQAEGDAYTPNGPAPFSMPRALALHEIPGVIEQFRQG
AARAKAAGFDGVEIHGANGYLPDQFLQDGTNHRTDAYGGPIENRARFLLEVTQAAIDVWGADRVGVRLSPSGTYGSMSDS
NPQETFGYVAEKLDEMGIAYLHVVEPRIKGTELVAESDAVAARHLRGAFSGTLIAAGGFDGASAEAIIRAGDADAVAFGR
AFISNPDLPERLRRNLPLNPYDRSTFYGGDAHGYTDYPALESVG

Sequences:

>Translated_364_residues
MSMLFTPFRAGALSLGHRVVMAPLTRMRSEPGDKAGALMREYYAQRTSHGGLIVSEATPVAREGYGYAGAPGIYDDAQIE
GWRTVTDAVHASGGKIVMQLWHVGRQSHRDLQPDGGAPVAPSAIQAEGDAYTPNGPAPFSMPRALALHEIPGVIEQFRQG
AARAKAAGFDGVEIHGANGYLPDQFLQDGTNHRTDAYGGPIENRARFLLEVTQAAIDVWGADRVGVRLSPSGTYGSMSDS
NPQETFGYVAEKLDEMGIAYLHVVEPRIKGTELVAESDAVAARHLRGAFSGTLIAAGGFDGASAEAIIRAGDADAVAFGR
AFISNPDLPERLRRNLPLNPYDRSTFYGGDAHGYTDYPALESVG
>Mature_363_residues
SMLFTPFRAGALSLGHRVVMAPLTRMRSEPGDKAGALMREYYAQRTSHGGLIVSEATPVAREGYGYAGAPGIYDDAQIEG
WRTVTDAVHASGGKIVMQLWHVGRQSHRDLQPDGGAPVAPSAIQAEGDAYTPNGPAPFSMPRALALHEIPGVIEQFRQGA
ARAKAAGFDGVEIHGANGYLPDQFLQDGTNHRTDAYGGPIENRARFLLEVTQAAIDVWGADRVGVRLSPSGTYGSMSDSN
PQETFGYVAEKLDEMGIAYLHVVEPRIKGTELVAESDAVAARHLRGAFSGTLIAAGGFDGASAEAIIRAGDADAVAFGRA
FISNPDLPERLRRNLPLNPYDRSTFYGGDAHGYTDYPALESVG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=51.5068493150685, Blast_Score=341, Evalue=4e-95,
Organism=Escherichia coli, GI1789463, Length=334, Percent_Identity=33.8323353293413, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI72001454, Length=254, Percent_Identity=28.740157480315, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=354, Percent_Identity=25.7062146892655, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=310, Percent_Identity=28.7096774193548, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=299, Percent_Identity=28.0936454849498, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=361, Percent_Identity=25.7617728531856, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=261, Percent_Identity=30.6513409961686, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=384, Percent_Identity=38.5416666666667, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6325086, Length=378, Percent_Identity=39.1534391534392, Blast_Score=228, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38771; Mature: 38639

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLFTPFRAGALSLGHRVVMAPLTRMRSEPGDKAGALMREYYAQRTSHGGLIVSEATPV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCH
AREGYGYAGAPGIYDDAQIEGWRTVTDAVHASGGKIVMQLWHVGRQSHRDLQPDGGAPVA
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PSAIQAEGDAYTPNGPAPFSMPRALALHEIPGVIEQFRQGAARAKAAGFDGVEIHGANGY
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
LPDQFLQDGTNHRTDAYGGPIENRARFLLEVTQAAIDVWGADRVGVRLSPSGTYGSMSDS
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
NPQETFGYVAEKLDEMGIAYLHVVEPRIKGTELVAESDAVAARHLRGAFSGTLIAAGGFD
CHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCEEEECCCC
GASAEAIIRAGDADAVAFGRAFISNPDLPERLRRNLPLNPYDRSTFYGGDAHGYTDYPAL
CCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
ESVG
HCCC
>Mature Secondary Structure 
SMLFTPFRAGALSLGHRVVMAPLTRMRSEPGDKAGALMREYYAQRTSHGGLIVSEATPV
CCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCH
AREGYGYAGAPGIYDDAQIEGWRTVTDAVHASGGKIVMQLWHVGRQSHRDLQPDGGAPVA
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PSAIQAEGDAYTPNGPAPFSMPRALALHEIPGVIEQFRQGAARAKAAGFDGVEIHGANGY
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
LPDQFLQDGTNHRTDAYGGPIENRARFLLEVTQAAIDVWGADRVGVRLSPSGTYGSMSDS
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
NPQETFGYVAEKLDEMGIAYLHVVEPRIKGTELVAESDAVAARHLRGAFSGTLIAAGGFD
CHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCEEEECCCC
GASAEAIIRAGDADAVAFGRAFISNPDLPERLRRNLPLNPYDRSTFYGGDAHGYTDYPAL
CCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
ESVG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]