Click here to switch to the map view.

The map label for this gene is rbsB [H]

Identifier: 162148407

GI number: 162148407

Start: 2717964

End: 2718914

Strand: Reverse

Name: rbsB [H]

Synonym: GDI_2626

Alternate gene names: 162148407

Gene position: 2718914-2717964 (Counterclockwise)

Preceding gene: 162148408

Following gene: 162148405

Centisome position: 68.94

GC content: 65.3

Gene sequence:

>951_bases
ATGACGTTCAAATCCGTCCTTCTCGCCGGCCTGGCTGTACTCTCGCTGGCCGCGCCCTCTCTGGCTGTGACTGCGGCCCA
GGCCAAGCCGATCACCGGCATCGGCGTTTCGCTGGGCACATTGGGCAACCCCTATTTCGTGGCGCTGGTCAAGGGCGTCA
CCGCCGAGGCGGCGAAGGTCGCGCCGAACGCGCGCATCACATCGGTTTCGGCCGACTACGATTTGAACAAGCAGTTCTCG
CAGATCGATAATTTCATCGCATCGGGGGACAATCTCCTGCTGATCAACGCGGTCGATCCGCAGGCCATCCTGCCGGCCAT
CAAGCGCGCGCAGAAAGCGGGCGCCGTGGTGGTGGTGGTGGACGTGGGCGCGGCCGGCGCCGACGCGGTCGTCCAGACCG
ACAACGTGGCGGCGGGGCGCCTGTCCTGCGAATACCTGGCGCAGAAGATCGGCGGCAAGGGCAACGTCGCGATCCAGAAC
GGCCCGCAGGTGTCCTCGGTCCTCGACCGCGTCAAAGGCTGCAAGGAAGCACTGGCCAAGAGCCCCGGCATTACGATCGT
GACCGACGACCAGAACGGCCAGGGCTCGCGCGATGCCGGATTCGCCGTCATGCAGGGCTATGTCGTGCGCTTTCCCGACC
TGGCCGGCGTGTTCACCATCAACGACCCGCAGGCCATCGGCAGCGACCTCGCGGCCCGGCAGGCGCATCGGTCGGGCATC
GTCATCACCTCGGTGGACGGCGCGCCGGATATCGTGACGGCGCTGAAGGACAAGACGTCGTCGGTCCTTGCATCGTCCAG
CCAGGACCCGTACCAGATGGGCATCGATGCCGTCATTGCCGGGCAGGACCTGCTGGACGACAAGCTGAAACGCGGCACGG
TCCAGTTGATCATGCCCAAGCTGATCACCCGCGATAACGTGGACAGCTATCAGGGCTGGACCAACCACTGA

Upstream 100 bases:

>100_bases
ATCGTCGCCGTGGCGCTCGACCGCTTCCGCCAGCCCGCCGCGCGCACCTGATCGCGGCGTCCTCCCACTTTTCCTTCCCC
CCCTCATCGGACCTGTCAAC

Downstream 100 bases:

>100_bases
TCCGATTGCTGGATTTCCGGGGGATGCGTTCCCCCGGAAATTGACGTCAGGTCGCCCAGTTTTCCTGAAAATCGCCATAC
AGCGTCAGGCCGCCATCGAC

Product: D-ribose-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTFKSVLLAGLAVLSLAAPSLAVTAAQAKPITGIGVSLGTLGNPYFVALVKGVTAEAAKVAPNARITSVSADYDLNKQFS
QIDNFIASGDNLLLINAVDPQAILPAIKRAQKAGAVVVVVDVGAAGADAVVQTDNVAAGRLSCEYLAQKIGGKGNVAIQN
GPQVSSVLDRVKGCKEALAKSPGITIVTDDQNGQGSRDAGFAVMQGYVVRFPDLAGVFTINDPQAIGSDLAARQAHRSGI
VITSVDGAPDIVTALKDKTSSVLASSSQDPYQMGIDAVIAGQDLLDDKLKRGTVQLIMPKLITRDNVDSYQGWTNH

Sequences:

>Translated_316_residues
MTFKSVLLAGLAVLSLAAPSLAVTAAQAKPITGIGVSLGTLGNPYFVALVKGVTAEAAKVAPNARITSVSADYDLNKQFS
QIDNFIASGDNLLLINAVDPQAILPAIKRAQKAGAVVVVVDVGAAGADAVVQTDNVAAGRLSCEYLAQKIGGKGNVAIQN
GPQVSSVLDRVKGCKEALAKSPGITIVTDDQNGQGSRDAGFAVMQGYVVRFPDLAGVFTINDPQAIGSDLAARQAHRSGI
VITSVDGAPDIVTALKDKTSSVLASSSQDPYQMGIDAVIAGQDLLDDKLKRGTVQLIMPKLITRDNVDSYQGWTNH
>Mature_315_residues
TFKSVLLAGLAVLSLAAPSLAVTAAQAKPITGIGVSLGTLGNPYFVALVKGVTAEAAKVAPNARITSVSADYDLNKQFSQ
IDNFIASGDNLLLINAVDPQAILPAIKRAQKAGAVVVVVDVGAAGADAVVQTDNVAAGRLSCEYLAQKIGGKGNVAIQNG
PQVSSVLDRVKGCKEALAKSPGITIVTDDQNGQGSRDAGFAVMQGYVVRFPDLAGVFTINDPQAIGSDLAARQAHRSGIV
ITSVDGAPDIVTALKDKTSSVLASSSQDPYQMGIDAVIAGQDLLDDKLKRGTVQLIMPKLITRDNVDSYQGWTNH

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=300, Percent_Identity=29.3333333333333, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790674, Length=193, Percent_Identity=29.0155440414508, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790526, Length=274, Percent_Identity=27.007299270073, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32622; Mature: 32490

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKSVLLAGLAVLSLAAPSLAVTAAQAKPITGIGVSLGTLGNPYFVALVKGVTAEAAKV
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEECCCCCCCEEEEEECCCCHHHHHC
APNARITSVSADYDLNKQFSQIDNFIASGDNLLLINAVDPQAILPAIKRAQKAGAVVVVV
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEE
DVGAAGADAVVQTDNVAAGRLSCEYLAQKIGGKGNVAIQNGPQVSSVLDRVKGCKEALAK
ECCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHC
SPGITIVTDDQNGQGSRDAGFAVMQGYVVRFPDLAGVFTINDPQAIGSDLAARQAHRSGI
CCCEEEEECCCCCCCCCCCCHHHHHCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCE
VITSVDGAPDIVTALKDKTSSVLASSSQDPYQMGIDAVIAGQDLLDDKLKRGTVQLIMPK
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCHHHHHHHHCCHHEEHHHH
LITRDNVDSYQGWTNH
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFKSVLLAGLAVLSLAAPSLAVTAAQAKPITGIGVSLGTLGNPYFVALVKGVTAEAAKV
CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEECCCCCCCEEEEEECCCCHHHHHC
APNARITSVSADYDLNKQFSQIDNFIASGDNLLLINAVDPQAILPAIKRAQKAGAVVVVV
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEE
DVGAAGADAVVQTDNVAAGRLSCEYLAQKIGGKGNVAIQNGPQVSSVLDRVKGCKEALAK
ECCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHC
SPGITIVTDDQNGQGSRDAGFAVMQGYVVRFPDLAGVFTINDPQAIGSDLAARQAHRSGI
CCCEEEEECCCCCCCCCCCCHHHHHCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCE
VITSVDGAPDIVTALKDKTSSVLASSSQDPYQMGIDAVIAGQDLLDDKLKRGTVQLIMPK
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCHHHHHHHHCCHHEEHHHH
LITRDNVDSYQGWTNH
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]