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The map label for this gene is rhtB [H]

Identifier: 162148405

GI number: 162148405

Start: 2716242

End: 2716877

Strand: Reverse

Name: rhtB [H]

Synonym: GDI_2624

Alternate gene names: 162148405

Gene position: 2716877-2716242 (Counterclockwise)

Preceding gene: 162148407

Following gene: 162148404

Centisome position: 68.88

GC content: 67.61

Gene sequence:

>636_bases
ATGCTGCCACCGACGACAAGCCTGCTTGCCTTTGCCCTCGTGGCGTTCGGGATGGCGCTGACGCCGGGTCCCAACATGAT
CTATCTGATTTCCCGATCGCTCTGCCAGGGACCGCGCGCCGGCCTGGTCTCGCTTGGCGGGGTGGCCGCCGGGTTCGTGT
TTTACATGCTGTGCGCGGCGTTCGGGGTTACGGCGCTCGTCATGGCGGTGCCGTATGCGTACGATGGCCTGCGGATGGGC
GGGGCGGCCTACCTGGTCTATCTGGCCTGGCAGGCCATCCGTCCGGGCGGGCGTTCGCCCTTTCAGGTGCGGAACCTGCC
GCCCGATTCCCCCCGGCGTCTGCTGGCGATGGGCTTCGTGACGAACCTGCTCAACCCGAAGATCGCCGTGATGTACCTGT
CGCTGCTTCCCCAGTTCGTCAATCCGGCGCAGGGGCACGTGCTGTCGCAGTCCCTCACGCTCGGCACGACGCAGATCGTC
ATCAGCGTCATGGTCAACACGGTCATCGCCCTGATGGCCGGTTCGATCGCGCTGTTCATGACGACCCGGCCGCGCTGGGC
CCTGGTCCAGCGCTGGGTCATGGCCAGCGTGCTGGGCGGCCTGGCGGTCAGGATGGCGACCGAGGCGCGGCGCTGA

Upstream 100 bases:

>100_bases
TGGAAACCTGCCGTGCGAATTATGCTGTATCCGGAATGATCGGGCGGCGCCTACACTGCCGGTCAGTCGCACAGGACCGG
ACGGAAAGGAAAACGACGGA

Downstream 100 bases:

>100_bases
CAGGATGGTTCAGGACGGCCCTGTCGCAAAAGCGCGACAGGTGCCACGCCGGCGGGTCACGACCGGGTGATCGCGATGAC
GAGGGCTCTGGCGCACGGGA

Product: transporter protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MLPPTTSLLAFALVAFGMALTPGPNMIYLISRSLCQGPRAGLVSLGGVAAGFVFYMLCAAFGVTALVMAVPYAYDGLRMG
GAAYLVYLAWQAIRPGGRSPFQVRNLPPDSPRRLLAMGFVTNLLNPKIAVMYLSLLPQFVNPAQGHVLSQSLTLGTTQIV
ISVMVNTVIALMAGSIALFMTTRPRWALVQRWVMASVLGGLAVRMATEARR

Sequences:

>Translated_211_residues
MLPPTTSLLAFALVAFGMALTPGPNMIYLISRSLCQGPRAGLVSLGGVAAGFVFYMLCAAFGVTALVMAVPYAYDGLRMG
GAAYLVYLAWQAIRPGGRSPFQVRNLPPDSPRRLLAMGFVTNLLNPKIAVMYLSLLPQFVNPAQGHVLSQSLTLGTTQIV
ISVMVNTVIALMAGSIALFMTTRPRWALVQRWVMASVLGGLAVRMATEARR
>Mature_211_residues
MLPPTTSLLAFALVAFGMALTPGPNMIYLISRSLCQGPRAGLVSLGGVAAGFVFYMLCAAFGVTALVMAVPYAYDGLRMG
GAAYLVYLAWQAIRPGGRSPFQVRNLPPDSPRRLLAMGFVTNLLNPKIAVMYLSLLPQFVNPAQGHVLSQSLTLGTTQIV
ISVMVNTVIALMAGSIALFMTTRPRWALVQRWVMASVLGGLAVRMATEARR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=173, Percent_Identity=31.2138728323699, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788099, Length=192, Percent_Identity=23.9583333333333, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786522, Length=123, Percent_Identity=28.4552845528455, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22576; Mature: 22576

Theoretical pI: Translated: 11.55; Mature: 11.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPPTTSLLAFALVAFGMALTPGPNMIYLISRSLCQGPRAGLVSLGGVAAGFVFYMLCAA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FGVTALVMAVPYAYDGLRMGGAAYLVYLAWQAIRPGGRSPFQVRNLPPDSPRRLLAMGFV
HHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHH
TNLLNPKIAVMYLSLLPQFVNPAQGHVLSQSLTLGTTQIVISVMVNTVIALMAGSIALFM
HHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
TTRPRWALVQRWVMASVLGGLAVRMATEARR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPPTTSLLAFALVAFGMALTPGPNMIYLISRSLCQGPRAGLVSLGGVAAGFVFYMLCAA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FGVTALVMAVPYAYDGLRMGGAAYLVYLAWQAIRPGGRSPFQVRNLPPDSPRRLLAMGFV
HHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHH
TNLLNPKIAVMYLSLLPQFVNPAQGHVLSQSLTLGTTQIVISVMVNTVIALMAGSIALFM
HHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
TTRPRWALVQRWVMASVLGGLAVRMATEARR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]