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The map label for this gene is gltX

Identifier: 162147995

GI number: 162147995

Start: 2264360

End: 2265691

Strand: Reverse

Name: gltX

Synonym: GDI_2211

Alternate gene names: 162147995

Gene position: 2265691-2264360 (Counterclockwise)

Preceding gene: 162147996

Following gene: 162147994

Centisome position: 57.44

GC content: 68.99

Gene sequence:

>1332_bases
ATGAAACTCCGTTTTGCGCCCAGCCCGACGGGCCTGATCCATGTCGGCAACGCCCGCCAGGCGATTGCCAACGCCCTGTA
CGCGCGCCGTCACGGCGGCACGTTCCAACTGCGCATCGACGATACCGACCGCGAGCGTTCGCGCGACGAGTATGTCGATG
CCCTGCATACCGACCTGGCCTGGCTGGGGATCACGTGGGACGAAACGTTCCGCCAGTCCGACCGGCTGGACCGCTATGCG
GCGGCGATCGAAACGCTGAAGGCGTCGGGCCGACTGTATCCGTGTTTCGAGAGCGAGCAGGAACTGGCGTCGAAGCGCGA
GGCCCGGATTCGCATGCGCAAGCCGCCGATCTATGACCGCGCGATGCTGCGCATGACGGCGGAACAGCGCGCCCAGGCCG
AGGCGAACGGCAAGGTGCCGTACTGGCGCTTCCGCCTGTCGGACCAGGACCGTGGCGTGGACGACATGGTCATGGGCCGC
TCGCAGGTCAAATTGCAGTCGATTTCCGACCCGGTCCTGGTCCGGGCCGACGGCACGGTGCTGTACACGCTGGCCTCGGT
CGTGGACGACATGGAAACCGGCGTGACCCACGTGCTGCGCGGCGAGGACCATCTGACCAACACCGGGGTGCAGATCGACA
TCGCCGAGGCCCTGGGCGGAAAGGTCGGCCAGTTCGCCTTCGCCCACCTGCCGCTGCTGCTGGACGAAGCCGGGGGCAAG
CTGTCCAAGCGGTTCGACGGGCTGTCGATCCGCGCGCTGCGGCAGGACGGGATCGATCCCGTCGCCATCGTGTCCTACCT
GGCGCGGCTGGGCAGCGCGGACGACCCGGCGCCGCTGTCGTTCGACGACCTGGCCGCCAGCTATGACGTGCGGCGAGTGT
CGCGCTCGGCGGCCCGTTTCGACATGCGGCAGCTTCTGGCCCTGAACCGGCGGGTCATGCACCAGATGCCCTTCGGCGCC
ATTCGCGACCGCCTGCCCGAGGGTGCGACCGAGGCGTTCTGGATGGCCGTGCGCGGCAATGTCGACATGGTATCCGAACT
GCGCCACTGGTGGGACGTCGTCGGCGGCGTGATCGTGCCGCCGGTCCAGGACGATGAAGGCGCCTACCTGCTGCAGGCGC
TGGCCCTGCTGCCGCCCGAACCGTGGGACGCGCAGACCTGGAAGGACTGGACCACCGCCGTCCGCGACGCCACCGGCCGC
AGCGGCAAATCGGTCTTCCACCCCCTGCGCGTCGCCCTGACCGGCGAGGAAGAGGGCCCCGAAATGCGCGACCTGCTGCC
CCTGATGGGCCATGACCGGGTGGCGGAACGGCTACGCATCGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
ATGGTCGAGGATGACGAGGACGAGACCCCGGAATAGACCGCATCCGGCGGCCTTTCGTCGCGGCTGGTCTTTCGGGCGCG
TCCGACGCATATAGGGCGGC

Downstream 100 bases:

>100_bases
ACAGGATGCCCGCGGCGTCCCGGGCCGCGTGAGGGGCCATGGCCATGCGTTCGAACGGACTGGAATTCTTCTGGGGAAAA
GCGCGTGCTGTTCCAGCGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKLRFAPSPTGLIHVGNARQAIANALYARRHGGTFQLRIDDTDRERSRDEYVDALHTDLAWLGITWDETFRQSDRLDRYA
AAIETLKASGRLYPCFESEQELASKREARIRMRKPPIYDRAMLRMTAEQRAQAEANGKVPYWRFRLSDQDRGVDDMVMGR
SQVKLQSISDPVLVRADGTVLYTLASVVDDMETGVTHVLRGEDHLTNTGVQIDIAEALGGKVGQFAFAHLPLLLDEAGGK
LSKRFDGLSIRALRQDGIDPVAIVSYLARLGSADDPAPLSFDDLAASYDVRRVSRSAARFDMRQLLALNRRVMHQMPFGA
IRDRLPEGATEAFWMAVRGNVDMVSELRHWWDVVGGVIVPPVQDDEGAYLLQALALLPPEPWDAQTWKDWTTAVRDATGR
SGKSVFHPLRVALTGEEEGPEMRDLLPLMGHDRVAERLRIAAR

Sequences:

>Translated_443_residues
MKLRFAPSPTGLIHVGNARQAIANALYARRHGGTFQLRIDDTDRERSRDEYVDALHTDLAWLGITWDETFRQSDRLDRYA
AAIETLKASGRLYPCFESEQELASKREARIRMRKPPIYDRAMLRMTAEQRAQAEANGKVPYWRFRLSDQDRGVDDMVMGR
SQVKLQSISDPVLVRADGTVLYTLASVVDDMETGVTHVLRGEDHLTNTGVQIDIAEALGGKVGQFAFAHLPLLLDEAGGK
LSKRFDGLSIRALRQDGIDPVAIVSYLARLGSADDPAPLSFDDLAASYDVRRVSRSAARFDMRQLLALNRRVMHQMPFGA
IRDRLPEGATEAFWMAVRGNVDMVSELRHWWDVVGGVIVPPVQDDEGAYLLQALALLPPEPWDAQTWKDWTTAVRDATGR
SGKSVFHPLRVALTGEEEGPEMRDLLPLMGHDRVAERLRIAAR
>Mature_443_residues
MKLRFAPSPTGLIHVGNARQAIANALYARRHGGTFQLRIDDTDRERSRDEYVDALHTDLAWLGITWDETFRQSDRLDRYA
AAIETLKASGRLYPCFESEQELASKREARIRMRKPPIYDRAMLRMTAEQRAQAEANGKVPYWRFRLSDQDRGVDDMVMGR
SQVKLQSISDPVLVRADGTVLYTLASVVDDMETGVTHVLRGEDHLTNTGVQIDIAEALGGKVGQFAFAHLPLLLDEAGGK
LSKRFDGLSIRALRQDGIDPVAIVSYLARLGSADDPAPLSFDDLAASYDVRRVSRSAARFDMRQLLALNRRVMHQMPFGA
IRDRLPEGATEAFWMAVRGNVDMVSELRHWWDVVGGVIVPPVQDDEGAYLLQALALLPPEPWDAQTWKDWTTAVRDATGR
SGKSVFHPLRVALTGEEEGPEMRDLLPLMGHDRVAERLRIAAR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=486, Percent_Identity=30.0411522633745, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4826960, Length=285, Percent_Identity=23.1578947368421, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=31.875, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI226510925, Length=249, Percent_Identity=32.1285140562249, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=27.8825995807128, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324540, Length=497, Percent_Identity=26.9617706237425, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6324742, Length=107, Percent_Identity=33.6448598130841, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=31.0838445807771, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_GLUDA (A9HLD9)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602456.1
- RefSeq:   YP_002274840.1
- ProteinModelPortal:   A9HLD9
- SMR:   A9HLD9
- GeneID:   5792212
- GeneID:   6973823
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0429
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 49681; Mature: 49681

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRFAPSPTGLIHVGNARQAIANALYARRHGGTFQLRIDDTDRERSRDEYVDALHTDLA
CCEECCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCHH
WLGITWDETFRQSDRLDRYAAAIETLKASGRLYPCFESEQELASKREARIRMRKPPIYDR
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHEEECCCCCHHH
AMLRMTAEQRAQAEANGKVPYWRFRLSDQDRGVDDMVMGRSQVKLQSISDPVLVRADGTV
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCHHHHHHHCCCCCEEEEECCHH
LYTLASVVDDMETGVTHVLRGEDHLTNTGVQIDIAEALGGKVGQFAFAHLPLLLDEAGGK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCH
LSKRFDGLSIRALRQDGIDPVAIVSYLARLGSADDPAPLSFDDLAASYDVRRVSRSAARF
HHHHCCCCEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DMRQLLALNRRVMHQMPFGAIRDRLPEGATEAFWMAVRGNVDMVSELRHWWDVVGGVIVP
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCEEEC
PVQDDEGAYLLQALALLPPEPWDAQTWKDWTTAVRDATGRSGKSVFHPLRVALTGEEEGP
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCEEEEEEECCCCCC
EMRDLLPLMGHDRVAERLRIAAR
HHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKLRFAPSPTGLIHVGNARQAIANALYARRHGGTFQLRIDDTDRERSRDEYVDALHTDLA
CCEECCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCHH
WLGITWDETFRQSDRLDRYAAAIETLKASGRLYPCFESEQELASKREARIRMRKPPIYDR
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHEEECCCCCHHH
AMLRMTAEQRAQAEANGKVPYWRFRLSDQDRGVDDMVMGRSQVKLQSISDPVLVRADGTV
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCHHHHHHHCCCCCEEEEECCHH
LYTLASVVDDMETGVTHVLRGEDHLTNTGVQIDIAEALGGKVGQFAFAHLPLLLDEAGGK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCH
LSKRFDGLSIRALRQDGIDPVAIVSYLARLGSADDPAPLSFDDLAASYDVRRVSRSAARF
HHHHCCCCEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DMRQLLALNRRVMHQMPFGAIRDRLPEGATEAFWMAVRGNVDMVSELRHWWDVVGGVIVP
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCEEEC
PVQDDEGAYLLQALALLPPEPWDAQTWKDWTTAVRDATGRSGKSVFHPLRVALTGEEEGP
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCEEEEEEECCCCCC
EMRDLLPLMGHDRVAERLRIAAR
HHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA