Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 162147583

GI number: 162147583

Start: 1830347

End: 1831288

Strand: Direct

Name: holB [H]

Synonym: GDI_1799

Alternate gene names: 162147583

Gene position: 1830347-1831288 (Clockwise)

Preceding gene: 162147582

Following gene: 162147584

Centisome position: 46.41

GC content: 69.75

Gene sequence:

>942_bases
CTGGTGGGGCAGCAGGAAGCGCGACGCGCGTTCGAGGACGCGCTTCGCACCGGGCGGCTGCATCACGCCTGGCTGCTGAC
CGGCACCGAGGGCATCGGCAAGGCGACGATGGCCTTCTGGATGGCGCGGCTGCTGCTGAAGGGGACGGACCCCGACAGTC
CCGCCGCACGGCGGATCGCCGCCGGCAGCCATGCCGACCTGCTGACCATCGCCCGGGGGGTGGATGAAAAGCGGCAGCGC
CTGCGCGCTGAAATCGTGGCCGACGACGTGCGGCCGATCGGCCAGTTCCTGCACCGCACGGCAGCCGAGGATGGCTGGCG
CGTCGTGATCGTGGACGGCGCGGAATACATGAACCGCAGCGCCGCCAACGCGATCCTGAAACTGCTGGAGGAACCGCCCC
CGCGCGCCATCCTGATCCTGACCTGCGCCACGCCGGGGCGCCTGCTGCCGACGATCCGCAGCCGGTGCCGGACGCTGAAG
CTGGGCCCGCTGTCGCCCGCCGACATGCATGCCGTCCTGTCCACGGTTGTCGAGGACGCGGCCCCCGACGACATCGCGCG
CGTCGTGCCGCTGGCCCATGGATCGCCGGGCCGGGCGCTGGCGTTGCTGGCGGGTGACGGCGGCGCGCTGGGCGCCCTGG
TCGCGCGCGTGATGGACGCGCCGGCAGGCCCGGGAGCCATGCGGGAAGGAGAGATGTACGAGATTGCGGAAGCGGTCCTG
AAGCGGGAGAATGGCTTTTCCGTCTTCTTCGATCTGCTGTGCGATGCCATATCGGTGCGCACCCGCGCCGCCGTGCGCGG
CACGATCCCGGCGGAAAGGCCGGCCGACCGCATGGTCGATCTCTGGCGTCGTCTGGTCCATCTGCGGGCCGAGACGGAAC
AGTTCAATCTGGATAAACATCAGGCCATCCTGAGCGGCCTGACATTGGTGAGTGGAATATGA

Upstream 100 bases:

>100_bases
GCGGCCGACCGGCCGGTGGATGTGGTCGCGGGCGACATCGCCCGCCTGGTGGACGCAAGGCTGGAAACGGATGCCCCCGG
CGCCGCGTGACGCTCCGGAC

Downstream 100 bases:

>100_bases
CCCGGCGCTATTACGTCACGACACCGATCTATTATGTGAACGGGGCGCCCCATATCGGCCACGCCTATACATCGGTCGCC
GCCGACATCGTGGCGCGGTT

Product: DNA polymerase III delta' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MVGQQEARRAFEDALRTGRLHHAWLLTGTEGIGKATMAFWMARLLLKGTDPDSPAARRIAAGSHADLLTIARGVDEKRQR
LRAEIVADDVRPIGQFLHRTAAEDGWRVVIVDGAEYMNRSAANAILKLLEEPPPRAILILTCATPGRLLPTIRSRCRTLK
LGPLSPADMHAVLSTVVEDAAPDDIARVVPLAHGSPGRALALLAGDGGALGALVARVMDAPAGPGAMREGEMYEIAEAVL
KRENGFSVFFDLLCDAISVRTRAAVRGTIPAERPADRMVDLWRRLVHLRAETEQFNLDKHQAILSGLTLVSGI

Sequences:

>Translated_313_residues
MVGQQEARRAFEDALRTGRLHHAWLLTGTEGIGKATMAFWMARLLLKGTDPDSPAARRIAAGSHADLLTIARGVDEKRQR
LRAEIVADDVRPIGQFLHRTAAEDGWRVVIVDGAEYMNRSAANAILKLLEEPPPRAILILTCATPGRLLPTIRSRCRTLK
LGPLSPADMHAVLSTVVEDAAPDDIARVVPLAHGSPGRALALLAGDGGALGALVARVMDAPAGPGAMREGEMYEIAEAVL
KRENGFSVFFDLLCDAISVRTRAAVRGTIPAERPADRMVDLWRRLVHLRAETEQFNLDKHQAILSGLTLVSGI
>Mature_313_residues
MVGQQEARRAFEDALRTGRLHHAWLLTGTEGIGKATMAFWMARLLLKGTDPDSPAARRIAAGSHADLLTIARGVDEKRQR
LRAEIVADDVRPIGQFLHRTAAEDGWRVVIVDGAEYMNRSAANAILKLLEEPPPRAILILTCATPGRLLPTIRSRCRTLK
LGPLSPADMHAVLSTVVEDAAPDDIARVVPLAHGSPGRALALLAGDGGALGALVARVMDAPAGPGAMREGEMYEIAEAVL
KRENGFSVFFDLLCDAISVRTRAAVRGTIPAERPADRMVDLWRRLVHLRAETEQFNLDKHQAILSGLTLVSGI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI194306571, Length=228, Percent_Identity=26.7543859649123, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI194306567, Length=228, Percent_Identity=26.7543859649123, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI6677723, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786676, Length=189, Percent_Identity=29.1005291005291, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787341, Length=201, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR022754
- InterPro:   IPR012763 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33853; Mature: 33853

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGQQEARRAFEDALRTGRLHHAWLLTGTEGIGKATMAFWMARLLLKGTDPDSPAARRIA
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AGSHADLLTIARGVDEKRQRLRAEIVADDVRPIGQFLHRTAAEDGWRVVIVDGAEYMNRS
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
AANAILKLLEEPPPRAILILTCATPGRLLPTIRSRCRTLKLGPLSPADMHAVLSTVVEDA
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHC
APDDIARVVPLAHGSPGRALALLAGDGGALGALVARVMDAPAGPGAMREGEMYEIAEAVL
CCHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
KRENGFSVFFDLLCDAISVRTRAAVRGTIPAERPADRMVDLWRRLVHLRAETEQFNLDKH
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
QAILSGLTLVSGI
HHHHHHHHHHHCC
>Mature Secondary Structure
MVGQQEARRAFEDALRTGRLHHAWLLTGTEGIGKATMAFWMARLLLKGTDPDSPAARRIA
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AGSHADLLTIARGVDEKRQRLRAEIVADDVRPIGQFLHRTAAEDGWRVVIVDGAEYMNRS
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
AANAILKLLEEPPPRAILILTCATPGRLLPTIRSRCRTLKLGPLSPADMHAVLSTVVEDA
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHC
APDDIARVVPLAHGSPGRALALLAGDGGALGALVARVMDAPAGPGAMREGEMYEIAEAVL
CCHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
KRENGFSVFFDLLCDAISVRTRAAVRGTIPAERPADRMVDLWRRLVHLRAETEQFNLDKH
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
QAILSGLTLVSGI
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]