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The map label for this gene is metG [H]

Identifier: 162147584

GI number: 162147584

Start: 1831285

End: 1832823

Strand: Direct

Name: metG [H]

Synonym: GDI_1800

Alternate gene names: 162147584

Gene position: 1831285-1832823 (Clockwise)

Preceding gene: 162147583

Following gene: 162147585

Centisome position: 46.43

GC content: 67.77

Gene sequence:

>1539_bases
ATGACCCGGCGCTATTACGTCACGACACCGATCTATTATGTGAACGGGGCGCCCCATATCGGCCACGCCTATACATCGGT
CGCCGCCGACATCGTGGCGCGGTTCAAGCGGCTGGCGGGTTTCGACGTCTTCTTCCTGACCGGCACGGACGAACACGGCC
AGAAGGTGGAACAGGCCGCCCAGGCGGCGGGCCTGGCCCCGATCGCGTTCGCGGACCGCGTTTCAGCCGATTTCCGCGCG
ATGTACGACGTGATGAACGTCTCGTACGACGATTTCATCCGCACCACCGAACCGCGTCACATCGCCGGCGCCACGGCCCT
GTGGGAGAGGATCGCGGCGAACGGCCATATCTACCTGGGTGCGTACGAGGGCTGGTACGCCCTGCGCGACGAAAGCTTCT
ATAACGAGGACGAACTGGTTACCCAGCCCGACGGCACCCGGGTCGCGCCGACCGGCGCCCCGGTCGAATGGGTCCGCGAG
CCGTCCTATTTCTTCCGCCTGTCCGCCTTCGCCGACCGGCTGCTGGACCTGTACGAAACCCGCCCGGGCTTCGTGGAGCC
CGCGTCGCGGCGCAACGAGGTCGCAAGCTTCGTCCGCCAGGGCCTGCGCGACCTGTCGATCAGCCGCACCAGCTTCCGCT
GGGGCATTCCGGTGCCCGGCGATGCCGATCACGTGATGTATGTCTGGGTCGACGCGCTGGCCAACTACCTGTCCGCGATC
GGCTTTCCCGACGCCCGCAACCCGCGCGCGGGCTTCTGGCCGGCGAACCTGCACCTGGTGGGCAAGGACATCGTCCGCTT
CCACGCCATCTACTGGCCGGCGCTGCTGATGGCGGCGGGTCTGGACCTGCCGGACTGCGTCTTCTCGCACGGATGGTGGA
CCATCGAGGGCGAGAAGATGAGCAAGTCGCTGGGCAATGTCGTCGATCCGCGCGACCTGGTGGCGGAATTCGGCCTGGAC
CCGGTGCGCTTCTTCCTGATGCGCGAAATGCCCTTCGGCGGTGACAGCGACCTGAGCCGCAAGGCCATCATCAACCGGAT
GAATGTCGAACTGGCCAACGACCTGGGCAACCTGGCGCAGCGCACGCTGTCGCAGGTGGCGCGCAATTGCGGCGGGCTGC
TGCCGGAGCAGGGCACGCGGACCGACGACGACATCGCGCTGCTGGCGCAGGCCGACCTGCTGCCGACCCTGATGCACGAA
CAGATCGACCGCCGCGCCCTGACCGACGCGCTGGAAGAGGTCTGGCGCGTCATCCGCGCCTGCAACGCCTATATCGACCG
CCAGGCCCCGTGGGCGCTGAAGAAGACCGATCCCGACCGGATGGCGGTGGTGCTGCGCGTGCTGGTCGATGCCCTGCGCG
GCATCGGCACCATGCTGCAGCCCTACATGCCCGGCAGCATGGATGCGCTGCTGACGCAGCTTGGCGTGGCGGAGGACGAG
CGCGATTTCGCGTCCCTGGCCACGCCGCTGCCGGGCGGACGCGCCCTGCCGCCGCCGCAGGGGATCTTTCCCCGTTACGT
GGAGGCCGAGGCGCAATGA

Upstream 100 bases:

>100_bases
ATCTCTGGCGTCGTCTGGTCCATCTGCGGGCCGAGACGGAACAGTTCAATCTGGATAAACATCAGGCCATCCTGAGCGGC
CTGACATTGGTGAGTGGAAT

Downstream 100 bases:

>100_bases
CGGGACTGATCGATTCGCACTGCCATCTGGACCATTTCACGGATGACGAGATCCCGGACCTGCTGGACAGGGCGCGCCAG
GCCGGGGTCGAAGGGATGGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTRRYYVTTPIYYVNGAPHIGHAYTSVAADIVARFKRLAGFDVFFLTGTDEHGQKVEQAAQAAGLAPIAFADRVSADFRA
MYDVMNVSYDDFIRTTEPRHIAGATALWERIAANGHIYLGAYEGWYALRDESFYNEDELVTQPDGTRVAPTGAPVEWVRE
PSYFFRLSAFADRLLDLYETRPGFVEPASRRNEVASFVRQGLRDLSISRTSFRWGIPVPGDADHVMYVWVDALANYLSAI
GFPDARNPRAGFWPANLHLVGKDIVRFHAIYWPALLMAAGLDLPDCVFSHGWWTIEGEKMSKSLGNVVDPRDLVAEFGLD
PVRFFLMREMPFGGDSDLSRKAIINRMNVELANDLGNLAQRTLSQVARNCGGLLPEQGTRTDDDIALLAQADLLPTLMHE
QIDRRALTDALEEVWRVIRACNAYIDRQAPWALKKTDPDRMAVVLRVLVDALRGIGTMLQPYMPGSMDALLTQLGVAEDE
RDFASLATPLPGGRALPPPQGIFPRYVEAEAQ

Sequences:

>Translated_512_residues
MTRRYYVTTPIYYVNGAPHIGHAYTSVAADIVARFKRLAGFDVFFLTGTDEHGQKVEQAAQAAGLAPIAFADRVSADFRA
MYDVMNVSYDDFIRTTEPRHIAGATALWERIAANGHIYLGAYEGWYALRDESFYNEDELVTQPDGTRVAPTGAPVEWVRE
PSYFFRLSAFADRLLDLYETRPGFVEPASRRNEVASFVRQGLRDLSISRTSFRWGIPVPGDADHVMYVWVDALANYLSAI
GFPDARNPRAGFWPANLHLVGKDIVRFHAIYWPALLMAAGLDLPDCVFSHGWWTIEGEKMSKSLGNVVDPRDLVAEFGLD
PVRFFLMREMPFGGDSDLSRKAIINRMNVELANDLGNLAQRTLSQVARNCGGLLPEQGTRTDDDIALLAQADLLPTLMHE
QIDRRALTDALEEVWRVIRACNAYIDRQAPWALKKTDPDRMAVVLRVLVDALRGIGTMLQPYMPGSMDALLTQLGVAEDE
RDFASLATPLPGGRALPPPQGIFPRYVEAEAQ
>Mature_511_residues
TRRYYVTTPIYYVNGAPHIGHAYTSVAADIVARFKRLAGFDVFFLTGTDEHGQKVEQAAQAAGLAPIAFADRVSADFRAM
YDVMNVSYDDFIRTTEPRHIAGATALWERIAANGHIYLGAYEGWYALRDESFYNEDELVTQPDGTRVAPTGAPVEWVREP
SYFFRLSAFADRLLDLYETRPGFVEPASRRNEVASFVRQGLRDLSISRTSFRWGIPVPGDADHVMYVWVDALANYLSAIG
FPDARNPRAGFWPANLHLVGKDIVRFHAIYWPALLMAAGLDLPDCVFSHGWWTIEGEKMSKSLGNVVDPRDLVAEFGLDP
VRFFLMREMPFGGDSDLSRKAIINRMNVELANDLGNLAQRTLSQVARNCGGLLPEQGTRTDDDIALLAQADLLPTLMHEQ
IDRRALTDALEEVWRVIRACNAYIDRQAPWALKKTDPDRMAVVLRVLVDALRGIGTMLQPYMPGSMDALLTQLGVAEDER
DFASLATPLPGGRALPPPQGIFPRYVEAEAQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=40.3345724907063, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI14043022, Length=514, Percent_Identity=29.1828793774319, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1788432, Length=508, Percent_Identity=25.9842519685039, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=35.4775828460039, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=32.6781326781327, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17540794, Length=551, Percent_Identity=26.497277676951, Blast_Score=158, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6321610, Length=547, Percent_Identity=32.5411334552102, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6321704, Length=510, Percent_Identity=24.9019607843137, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=38.3697813121272, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655311, Length=554, Percent_Identity=27.7978339350181, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57162; Mature: 57030

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRYYVTTPIYYVNGAPHIGHAYTSVAADIVARFKRLAGFDVFFLTGTDEHGQKVEQAA
CCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
QAAGLAPIAFADRVSADFRAMYDVMNVSYDDFIRTTEPRHIAGATALWERIAANGHIYLG
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
AYEGWYALRDESFYNEDELVTQPDGTRVAPTGAPVEWVREPSYFFRLSAFADRLLDLYET
EECCEEEECCCCCCCCCCEEECCCCCEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHC
RPGFVEPASRRNEVASFVRQGLRDLSISRTSFRWGIPVPGDADHVMYVWVDALANYLSAI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GFPDARNPRAGFWPANLHLVGKDIVRFHAIYWPALLMAAGLDLPDCVFSHGWWTIEGEKM
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEHHHH
SKSLGNVVDPRDLVAEFGLDPVRFFLMREMPFGGDSDLSRKAIINRMNVELANDLGNLAQ
HHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RTLSQVARNCGGLLPEQGTRTDDDIALLAQADLLPTLMHEQIDRRALTDALEEVWRVIRA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CNAYIDRQAPWALKKTDPDRMAVVLRVLVDALRGIGTMLQPYMPGSMDALLTQLGVAEDE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCH
RDFASLATPLPGGRALPPPQGIFPRYVEAEAQ
HHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
>Mature Secondary Structure 
TRRYYVTTPIYYVNGAPHIGHAYTSVAADIVARFKRLAGFDVFFLTGTDEHGQKVEQAA
CCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
QAAGLAPIAFADRVSADFRAMYDVMNVSYDDFIRTTEPRHIAGATALWERIAANGHIYLG
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
AYEGWYALRDESFYNEDELVTQPDGTRVAPTGAPVEWVREPSYFFRLSAFADRLLDLYET
EECCEEEECCCCCCCCCCEEECCCCCEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHC
RPGFVEPASRRNEVASFVRQGLRDLSISRTSFRWGIPVPGDADHVMYVWVDALANYLSAI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GFPDARNPRAGFWPANLHLVGKDIVRFHAIYWPALLMAAGLDLPDCVFSHGWWTIEGEKM
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEHHHH
SKSLGNVVDPRDLVAEFGLDPVRFFLMREMPFGGDSDLSRKAIINRMNVELANDLGNLAQ
HHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RTLSQVARNCGGLLPEQGTRTDDDIALLAQADLLPTLMHEQIDRRALTDALEEVWRVIRA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CNAYIDRQAPWALKKTDPDRMAVVLRVLVDALRGIGTMLQPYMPGSMDALLTQLGVAEDE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCH
RDFASLATPLPGGRALPPPQGIFPRYVEAEAQ
HHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]