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The map label for this gene is emrA [H]

Identifier: 162147398

GI number: 162147398

Start: 1619887

End: 1621038

Strand: Reverse

Name: emrA [H]

Synonym: GDI_1613

Alternate gene names: 162147398

Gene position: 1621038-1619887 (Counterclockwise)

Preceding gene: 162147399

Following gene: 162147397

Centisome position: 41.1

GC content: 67.53

Gene sequence:

>1152_bases
ATGGCGGATGAAGACCGGAACGGAAACCGTGGTCACACGGACAGCAAGCAGAAGCATGGCAGTCCCGTCATGCGCGGGCT
CTTCATTGGCGGAGGTGCGGTCGTCGTCGTTTTCATCGCTGTCGTCCTCGCCATTATTTTCGTGCCCACACGCCGCGTCT
GGACCGATGACGCCTACGTCACCGCCCATTACACCGTCGTGGCCCCCCGCGTGTCCGGGCAGGTCGTCGCGGTGAATGTG
ACGGACAACCAGTCTGTCAAGGCGGGCCAGGTCCTGGTGGAACTGGATCCGCGCGATTACCGGACCGCGCTGGACCAGGC
CCGGGCCACCCTTGAACGGGACCGCTCGCTGGTGCAGGACGCCGCCAGTGCCGTGACGCGCCAGCCGTCGATCATCGACG
AAAGCGTCGGGGCCGCCGCCCGTGTCCGGGCCCAGCTTATCTTTGCCCGCCAGAACGCCCAGCGTTACGCCAACCTGGCG
CGGACCGGCGCCGGTTCGACGCAGGAAAACCAGCAATCCACCGCAACGCTGCGGGACATGGAGGCGAACCTCCAGTCGCT
CGAGGCCCGCGTCGCCGCCGCCCGGGCGCAGCTTCCCATCCTGCAGGCCCGCCACAAGGCCGCGCAGGCGACGGTGGACC
TCGATAATGCCAGGGTCCGCCAGGCCGAGCTCAACCTGTCCTACACCCGTATCCGCGCCCTGGAAGACGGCATGATCGGC
GAGCGCAGCGTCCAGATCGGCAACTACGTGTCCCCCGGCGCGGCGCTGATGGCGCTTGTCCCGCTCGACCAGATCTGGAT
CCGCGCCAACTACCGTGAACTGGCCCTGCGCCATATGCGCCCCGGGCAGCCCGTCCGGATCCACGTCGATGCCTATGACG
TCACCCTGAACGGGATCGTGGACAGCATTCCCCCGGCCTCCGGCGCGGTTTTCGCCCCCATCGCGCCGGAAAACGCCACC
GGCAACTTCACCAAGATCGTGCAGCGCCTGCCGGTCAAAATCATCCTCGCTCCGAACCAGCCCCTGGCGAAGCTGCTTCG
TCTCGGCTTCTCGGTCGAAACGACGGTCGATACCGGCTTTGCCGATGTCGTGGGCGCGCAGGGGCACGATGCGGCCGCGA
CCGTCACCGGCCGCGAGGGTGCCGGCCCATGA

Upstream 100 bases:

>100_bases
CCTTCCGGTCCGAACCTATCCCCCCCGTATCGTGTTCCAGTCGAAATAGTCCGCGGACGCCCGCCTGTCCCCGTCGGAAG
CAGAAAGGAAACATCGCCGC

Downstream 100 bases:

>100_bases
AACGGGCCGTCCCGCGTGTGGCCGCGTTCGCCCTGCCGCTGGCCATCGCCCTGGCAGGCTGTACCGTCGGCCCGGCCTTC
CACCGCCCCCGGATCGACGC

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MADEDRNGNRGHTDSKQKHGSPVMRGLFIGGGAVVVVFIAVVLAIIFVPTRRVWTDDAYVTAHYTVVAPRVSGQVVAVNV
TDNQSVKAGQVLVELDPRDYRTALDQARATLERDRSLVQDAASAVTRQPSIIDESVGAAARVRAQLIFARQNAQRYANLA
RTGAGSTQENQQSTATLRDMEANLQSLEARVAAARAQLPILQARHKAAQATVDLDNARVRQAELNLSYTRIRALEDGMIG
ERSVQIGNYVSPGAALMALVPLDQIWIRANYRELALRHMRPGQPVRIHVDAYDVTLNGIVDSIPPASGAVFAPIAPENAT
GNFTKIVQRLPVKIILAPNQPLAKLLRLGFSVETTVDTGFADVVGAQGHDAAATVTGREGAGP

Sequences:

>Translated_383_residues
MADEDRNGNRGHTDSKQKHGSPVMRGLFIGGGAVVVVFIAVVLAIIFVPTRRVWTDDAYVTAHYTVVAPRVSGQVVAVNV
TDNQSVKAGQVLVELDPRDYRTALDQARATLERDRSLVQDAASAVTRQPSIIDESVGAAARVRAQLIFARQNAQRYANLA
RTGAGSTQENQQSTATLRDMEANLQSLEARVAAARAQLPILQARHKAAQATVDLDNARVRQAELNLSYTRIRALEDGMIG
ERSVQIGNYVSPGAALMALVPLDQIWIRANYRELALRHMRPGQPVRIHVDAYDVTLNGIVDSIPPASGAVFAPIAPENAT
GNFTKIVQRLPVKIILAPNQPLAKLLRLGFSVETTVDTGFADVVGAQGHDAAATVTGREGAGP
>Mature_382_residues
ADEDRNGNRGHTDSKQKHGSPVMRGLFIGGGAVVVVFIAVVLAIIFVPTRRVWTDDAYVTAHYTVVAPRVSGQVVAVNVT
DNQSVKAGQVLVELDPRDYRTALDQARATLERDRSLVQDAASAVTRQPSIIDESVGAAARVRAQLIFARQNAQRYANLAR
TGAGSTQENQQSTATLRDMEANLQSLEARVAAARAQLPILQARHKAAQATVDLDNARVRQAELNLSYTRIRALEDGMIGE
RSVQIGNYVSPGAALMALVPLDQIWIRANYRELALRHMRPGQPVRIHVDAYDVTLNGIVDSIPPASGAVFAPIAPENATG
NFTKIVQRLPVKIILAPNQPLAKLLRLGFSVETTVDTGFADVVGAQGHDAAATVTGREGAGP

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=356, Percent_Identity=34.2696629213483, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789041, Length=341, Percent_Identity=33.4310850439883, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790519, Length=307, Percent_Identity=29.6416938110749, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789637, Length=314, Percent_Identity=28.6624203821656, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081951, Length=314, Percent_Identity=26.1146496815287, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41151; Mature: 41020

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEDRNGNRGHTDSKQKHGSPVMRGLFIGGGAVVVVFIAVVLAIIFVPTRRVWTDDAYV
CCCCCCCCCCCCCCCHHHCCCHHHHEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCCEE
TAHYTVVAPRVSGQVVAVNVTDNQSVKAGQVLVELDPRDYRTALDQARATLERDRSLVQD
EEEEEEEEECCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
AASAVTRQPSIIDESVGAAARVRAQLIFARQNAQRYANLARTGAGSTQENQQSTATLRDM
HHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
EANLQSLEARVAAARAQLPILQARHKAAQATVDLDNARVRQAELNLSYTRIRALEDGMIG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEHHHCCCHHHHHHHHHCCCCC
ERSVQIGNYVSPGAALMALVPLDQIWIRANYRELALRHMRPGQPVRIHVDAYDVTLNGIV
CCEEECCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHCCCCCCEEEEEEEEEEEEECHH
DSIPPASGAVFAPIAPENATGNFTKIVQRLPVKIILAPNQPLAKLLRLGFSVETTVDTGF
HCCCCCCCCEEECCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHCCCCEEECCCCCH
ADVVGAQGHDAAATVTGREGAGP
HHHCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
ADEDRNGNRGHTDSKQKHGSPVMRGLFIGGGAVVVVFIAVVLAIIFVPTRRVWTDDAYV
CCCCCCCCCCCCCCHHHCCCHHHHEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCCEE
TAHYTVVAPRVSGQVVAVNVTDNQSVKAGQVLVELDPRDYRTALDQARATLERDRSLVQD
EEEEEEEEECCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
AASAVTRQPSIIDESVGAAARVRAQLIFARQNAQRYANLARTGAGSTQENQQSTATLRDM
HHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
EANLQSLEARVAAARAQLPILQARHKAAQATVDLDNARVRQAELNLSYTRIRALEDGMIG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEHHHCCCHHHHHHHHHCCCCC
ERSVQIGNYVSPGAALMALVPLDQIWIRANYRELALRHMRPGQPVRIHVDAYDVTLNGIV
CCEEECCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHCCCCCCEEEEEEEEEEEEECHH
DSIPPASGAVFAPIAPENATGNFTKIVQRLPVKIILAPNQPLAKLLRLGFSVETTVDTGF
HCCCCCCCCEEECCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHCCCCEEECCCCCH
ADVVGAQGHDAAATVTGREGAGP
HHHCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]