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The map label for this gene is oprM [H]

Identifier: 162147397

GI number: 162147397

Start: 1618442

End: 1619890

Strand: Reverse

Name: oprM [H]

Synonym: GDI_1612

Alternate gene names: 162147397

Gene position: 1619890-1618442 (Counterclockwise)

Preceding gene: 162147398

Following gene: 162147394

Centisome position: 41.07

GC content: 68.67

Gene sequence:

>1449_bases
ATGAAACGGGCCGTCCCGCGTGTGGCCGCGTTCGCCCTGCCGCTGGCCATCGCCCTGGCAGGCTGTACCGTCGGCCCGGC
CTTCCACCGCCCCCGGATCGACGCCCCGCCGGCCTGGGGCGCCGAACCGAAGGCCGGAAGCCAGACCTATGCCGGCGCCA
TCGACACCCGGTGGTGGCGCAGCTTCGACGACGCGGAACTGACCGGCCTGGTGGACCGGCTCGGCCGGCAGAACCTCGAC
CTCCAGATCGCGGCCCAGCGAATCCGTGAAGCGCGCGCGCAGATGAAGGCCGTCGCCGCCGCCGGACTGCCGCAGGCCAA
CTGGTCGGGTTCCTACGCCTATCGCCAACTCAGCACGCACGGCATTTTCTCGCTGGCCGAACCAAGCCCCGGCGCCAGCC
TGAACTTCAACCTGTACAGCAATGTCCTCAGCACCAGCTGGGACCTGGACCTGTTCGGCGCCATTCGTCGCGGGGTCGAG
GCCCAGCGTGCCGATCAGTCCGCCGCCCTTGCCGCCCGCCGTGCGGCCGCCCTGGCCGCCGTCAGCGACCTTGCCGCCAG
CTACATGCAGCTTCGCGGTGTGCAGGCGCAGCAGGTCATCCTGCAGTCCAACATCGCTCTCGCCCGGACCAATCTGCAAC
TGGTGCGGGACCGCTTCGCCAACGGGGTCGCGGCGAACCTCGATGTCTCGCAGGCGGCTGCCCAGCTTGCCAGCATGGAA
TCCGGCCTGCCCGAACTGGAAAATGCCGAGGCCGCCCTGATCAACGCCATCGGCCTGCTTCTGTCCGAGCCACCCCGCGC
GCTGGAAGGCGAACTGAAAAACCGGTCGCCCCAGCCACGCCTTCCCGTCAGTGTTCCCGTCGGCCTGCCTCTGGACCTGA
CGCGTCGCCGGCCGGATATTCTGGAAGCCGAGGCACGCCTTCATGCCGCCACGGCGGAAGTGGGCGCTGCCATCGCCGCC
TTCTATCCGGACATCACGCTTGCCGGAAACATGGGCACGGAATCCTTCGCGGCCTCGGATTTCTTTTCTCTTCCCGCCAA
GCAGTTCAGTGTCGGCCCCACCCTGAACGTTCCGGTCTTCCAGGGCGGGCGGCTGATCGCCAGGCTGGCGTTCCGCAAGG
CGGCCCAGCAGGAAGCCGTCCTGACCTGGCGCAATACCGTCCTGAACGCCTGGCGTGAGGCCGATGATGCCCTGACCGCC
TATGCCAAGTCCCAATCCACCCATGCCCTGACCGAGCAGGCCCTGCTGCAGGACCGCGCCGCCTACACGGCCGCACGGCA
ACGCTACGGCGAGGGGGCGACGGATTACCTGAACGTCGTCGCGACGCAGGCCGCCCTGCTGTCCACCCAGTCCGCCCTTG
CCGACAGCCAGACACGCTCGGAAACCACGCTCGTGGCCCTGTATCGTGCGCTTGGCGGGGGATGGGAGTATGCCGAGCCG
GGGCGATAG

Upstream 100 bases:

>100_bases
TCTCGGTCGAAACGACGGTCGATACCGGCTTTGCCGATGTCGTGGGCGCGCAGGGGCACGATGCGGCCGCGACCGTCACC
GGCCGCGAGGGTGCCGGCCC

Downstream 100 bases:

>100_bases
GGTTTGTTCTACGGATCGCTTCGCTCGGCCGCGCTCGCCGCGCGAATGCCGGCGAATTCCGATCCGGGTTTCCACTGGGG
CCAGGTGTGCGAGAACGCGA

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKRAVPRVAAFALPLAIALAGCTVGPAFHRPRIDAPPAWGAEPKAGSQTYAGAIDTRWWRSFDDAELTGLVDRLGRQNLD
LQIAAQRIREARAQMKAVAAAGLPQANWSGSYAYRQLSTHGIFSLAEPSPGASLNFNLYSNVLSTSWDLDLFGAIRRGVE
AQRADQSAALAARRAAALAAVSDLAASYMQLRGVQAQQVILQSNIALARTNLQLVRDRFANGVAANLDVSQAAAQLASME
SGLPELENAEAALINAIGLLLSEPPRALEGELKNRSPQPRLPVSVPVGLPLDLTRRRPDILEAEARLHAATAEVGAAIAA
FYPDITLAGNMGTESFAASDFFSLPAKQFSVGPTLNVPVFQGGRLIARLAFRKAAQQEAVLTWRNTVLNAWREADDALTA
YAKSQSTHALTEQALLQDRAAYTAARQRYGEGATDYLNVVATQAALLSTQSALADSQTRSETTLVALYRALGGGWEYAEP
GR

Sequences:

>Translated_482_residues
MKRAVPRVAAFALPLAIALAGCTVGPAFHRPRIDAPPAWGAEPKAGSQTYAGAIDTRWWRSFDDAELTGLVDRLGRQNLD
LQIAAQRIREARAQMKAVAAAGLPQANWSGSYAYRQLSTHGIFSLAEPSPGASLNFNLYSNVLSTSWDLDLFGAIRRGVE
AQRADQSAALAARRAAALAAVSDLAASYMQLRGVQAQQVILQSNIALARTNLQLVRDRFANGVAANLDVSQAAAQLASME
SGLPELENAEAALINAIGLLLSEPPRALEGELKNRSPQPRLPVSVPVGLPLDLTRRRPDILEAEARLHAATAEVGAAIAA
FYPDITLAGNMGTESFAASDFFSLPAKQFSVGPTLNVPVFQGGRLIARLAFRKAAQQEAVLTWRNTVLNAWREADDALTA
YAKSQSTHALTEQALLQDRAAYTAARQRYGEGATDYLNVVATQAALLSTQSALADSQTRSETTLVALYRALGGGWEYAEP
GR
>Mature_482_residues
MKRAVPRVAAFALPLAIALAGCTVGPAFHRPRIDAPPAWGAEPKAGSQTYAGAIDTRWWRSFDDAELTGLVDRLGRQNLD
LQIAAQRIREARAQMKAVAAAGLPQANWSGSYAYRQLSTHGIFSLAEPSPGASLNFNLYSNVLSTSWDLDLFGAIRRGVE
AQRADQSAALAARRAAALAAVSDLAASYMQLRGVQAQQVILQSNIALARTNLQLVRDRFANGVAANLDVSQAAAQLASME
SGLPELENAEAALINAIGLLLSEPPRALEGELKNRSPQPRLPVSVPVGLPLDLTRRRPDILEAEARLHAATAEVGAAIAA
FYPDITLAGNMGTESFAASDFFSLPAKQFSVGPTLNVPVFQGGRLIARLAFRKAAQQEAVLTWRNTVLNAWREADDALTA
YAKSQSTHALTEQALLQDRAAYTAARQRYGEGATDYLNVVATQAALLSTQSALADSQTRSETTLVALYRALGGGWEYAEP
GR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=29.247311827957, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI2367348, Length=434, Percent_Identity=24.4239631336406, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51511; Mature: 51511

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAVPRVAAFALPLAIALAGCTVGPAFHRPRIDAPPAWGAEPKAGSQTYAGAIDTRWWR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHC
SFDDAELTGLVDRLGRQNLDLQIAAQRIREARAQMKAVAAAGLPQANWSGSYAYRQLSTH
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
GIFSLAEPSPGASLNFNLYSNVLSTSWDLDLFGAIRRGVEAQRADQSAALAARRAAALAA
CCEEECCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSDLAASYMQLRGVQAQQVILQSNIALARTNLQLVRDRFANGVAANLDVSQAAAQLASME
HHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SGLPELENAEAALINAIGLLLSEPPRALEGELKNRSPQPRLPVSVPVGLPLDLTRRRPDI
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCH
LEAEARLHAATAEVGAAIAAFYPDITLAGNMGTESFAASDFFSLPAKQFSVGPTLNVPVF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCC
QGGRLIARLAFRKAAQQEAVLTWRNTVLNAWREADDALTAYAKSQSTHALTEQALLQDRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AYTAARQRYGEGATDYLNVVATQAALLSTQSALADSQTRSETTLVALYRALGGGWEYAEP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
GR
CC
>Mature Secondary Structure
MKRAVPRVAAFALPLAIALAGCTVGPAFHRPRIDAPPAWGAEPKAGSQTYAGAIDTRWWR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHC
SFDDAELTGLVDRLGRQNLDLQIAAQRIREARAQMKAVAAAGLPQANWSGSYAYRQLSTH
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
GIFSLAEPSPGASLNFNLYSNVLSTSWDLDLFGAIRRGVEAQRADQSAALAARRAAALAA
CCEEECCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSDLAASYMQLRGVQAQQVILQSNIALARTNLQLVRDRFANGVAANLDVSQAAAQLASME
HHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SGLPELENAEAALINAIGLLLSEPPRALEGELKNRSPQPRLPVSVPVGLPLDLTRRRPDI
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCH
LEAEARLHAATAEVGAAIAAFYPDITLAGNMGTESFAASDFFSLPAKQFSVGPTLNVPVF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCC
QGGRLIARLAFRKAAQQEAVLTWRNTVLNAWREADDALTAYAKSQSTHALTEQALLQDRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AYTAARQRYGEGATDYLNVVATQAALLSTQSALADSQTRSETTLVALYRALGGGWEYAEP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]