Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 162146621

GI number: 162146621

Start: 818383

End: 818889

Strand: Direct

Name: ruvC

Synonym: GDI_0799

Alternate gene names: 162146621

Gene position: 818383-818889 (Clockwise)

Preceding gene: 162146620

Following gene: 162146622

Centisome position: 20.75

GC content: 72.39

Gene sequence:

>507_bases
ATGGTCCGGCTGCTGGGCATCGATCCGGGCCTGCGCTTCACCGGCTGGGGGCTGGTCGATGTGGACGGCAACCGGCTGTG
CCATGTCGCGGACGGCGTGATCGCCACGGACGGTGACGCGCCCGTGCCGGAGCGGCTGCGCTGCCTGCACGATTCCCTGC
TGGACCTCGTGCGCCGGTACGGCCCGCGCGAGGCCGCGGTGGAGGAGACGTACGTCAACCGCAACGGGGCCTCGACGCTG
AAGCTCGGCTATGCGCGCGGGGTGGCGCTGCTGGTGCCGGCGCTGGCCGGAATCGCGGTTTCGGAATACGGTGCCATGGC
GGTGAAGCGGGCGGTGGTCGGCACCGGCGCCGCCAGCAAGGATCAGGTGGAAATGATGGTGCGCCGTCTGCTGCCCGGGG
CGACGATCCGCCGGGCGGACGCGTCCGATGCGCTGGCGGTGGCGATCTGCCATGCCCATCACCGGGCCAGCGCCCTGCGG
GTGTCGGCCGGGACCCGCATGGCATGA

Upstream 100 bases:

>100_bases
AACATGACGACGTCCAGGCGGTCTACGCCAATTTCGACCTGCCCGACGACGTGGCCGAGAGGCTGTCCGCCTGACGCCCG
CCACGGGGCCGAATGGACGG

Downstream 100 bases:

>100_bases
TCGCGCACCTGACCGGGCTGGTGGGGCAGATGGAATCCGACCGCTGCGTGGTCGATGTCGGCGGCGTCGGCTATCTGGTC
CATGCCTCCACCCGCACGCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MVRLLGIDPGLRFTGWGLVDVDGNRLCHVADGVIATDGDAPVPERLRCLHDSLLDLVRRYGPREAAVEETYVNRNGASTL
KLGYARGVALLVPALAGIAVSEYGAMAVKRAVVGTGAASKDQVEMMVRRLLPGATIRRADASDALAVAICHAHHRASALR
VSAGTRMA

Sequences:

>Translated_168_residues
MVRLLGIDPGLRFTGWGLVDVDGNRLCHVADGVIATDGDAPVPERLRCLHDSLLDLVRRYGPREAAVEETYVNRNGASTL
KLGYARGVALLVPALAGIAVSEYGAMAVKRAVVGTGAASKDQVEMMVRRLLPGATIRRADASDALAVAICHAHHRASALR
VSAGTRMA
>Mature_168_residues
MVRLLGIDPGLRFTGWGLVDVDGNRLCHVADGVIATDGDAPVPERLRCLHDSLLDLVRRYGPREAAVEETYVNRNGASTL
KLGYARGVALLVPALAGIAVSEYGAMAVKRAVVGTGAASKDQVEMMVRRLLPGATIRRADASDALAVAICHAHHRASALR
VSAGTRMA

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=162, Percent_Identity=40.7407407407407, Blast_Score=103, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_GLUDA (A9HAV5)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601080.1
- RefSeq:   YP_002275616.1
- ProteinModelPortal:   A9HAV5
- SMR:   A9HAV5
- GeneID:   5788773
- GeneID:   6974622
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1218
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17751; Mature: 17751

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLLGIDPGLRFTGWGLVDVDGNRLCHVADGVIATDGDAPVPERLRCLHDSLLDLVRRY
CEEEEECCCCCEEECCEEEEECCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHC
GPREAAVEETYVNRNGASTLKLGYARGVALLVPALAGIAVSEYGAMAVKRAVVGTGAASK
CCCHHHHHHHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DQVEMMVRRLLPGATIRRADASDALAVAICHAHHRASALRVSAGTRMA
HHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCC
>Mature Secondary Structure
MVRLLGIDPGLRFTGWGLVDVDGNRLCHVADGVIATDGDAPVPERLRCLHDSLLDLVRRY
CEEEEECCCCCEEECCEEEEECCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHC
GPREAAVEETYVNRNGASTLKLGYARGVALLVPALAGIAVSEYGAMAVKRAVVGTGAASK
CCCHHHHHHHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DQVEMMVRRLLPGATIRRADASDALAVAICHAHHRASALRVSAGTRMA
HHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA