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The map label for this gene is nadB [H]

Identifier: 162146396

GI number: 162146396

Start: 579560

End: 581116

Strand: Direct

Name: nadB [H]

Synonym: GDI_0571

Alternate gene names: 162146396

Gene position: 579560-581116 (Clockwise)

Preceding gene: 162146395

Following gene: 162146397

Centisome position: 14.69

GC content: 75.08

Gene sequence:

>1557_bases
ATGACCGGTCCGGGCCTCACCGACGCCGGTTCCTGGGCCGGCCAGCCGGTCATCGCCGGGGCGGGGCTGGCCGGGCTGGC
GACGGCGCTGCTGATGGACCGTCCCTGCGTGGTGCTGTCGCCCGCGCCGCTGGCGACGGACGCCGCCAGCGCGCTGGCCC
AGGGCGGAATCGCCGCCGCGATCGGCCCGGACGACACGCCCGCCCTGCATGCGCGCGACACGCTGGAGGCCGGCGTCGGC
CTGTGCGACCCCGTAGTGGTGGACGCCATCACCCGCGCCGGCCCTGGCGCCATCGCCGCGTTGCGCGACCTGGGCGTGGC
GTTCGATACCGGCCCTGACGGCAGGCTGGACCTGCACCTGGAAGCGGCCCATTCCCGCCCGCGCATCGCCCACGCCCATG
GTGACGGCACGGGGGCGGAAATCATGCGCGCCCTGGTCGCGCGCACCCGTGCCACCCCGCGCATCACGGTGCTGGACGGC
GCGGCCCTGCGCCGCGTGATCGTGCGCGACGGACGGGTCGCGGGGGTGCGGATCGCGCGGGCCGGGCAGGAGCTGATCCT
GCCGACATCGGCCTGCGTCATCGCCACCGGTGGCGTCGGCGCGCTGTTTCCCACCACCACCAGCCCGCCCGGCGGGCTGG
GGTCCGGCCTGGCCTGCGCCGCCCGCGCGGGCGCGGTGCTGCGCGACCTGGAATTCGTGCAGTTCCATCCCACCGCGCTG
GCGGTCGGGTCGGACGCGGCCCCCCGCGTACTGATCAGTGAGGCCGTGCGGGGGGCGGGCGCGATCCTGGTCGATGAAAG
CGGCGCGCGTTTCACCGACGAACTGGCGCCGCGCGACATTGTGGCCCGCGCGATCCAGGCGCATCTGTCGGCCGGCCACC
GCGTCCTGCTGGATGCCCGGGCCGCCACGCAGGGCCACTTCGCCCGGCATTTCCCCGCCATCGCGCGGGCCTGTGTCCAA
CACGGCATCGACCCCGACCGGCAGCCGATTCCGGTGCGTCCGGCGGTGCACTACCATATGGGCGGGATCGAGACCGACCT
GCGCGGCCGCAGCACCGTCCCCGGCCTGTGGGCGTGCGGCGAGGCCGCGTCGACCGGCCTACATGGGGCCAACCGGCTGG
CCAGTAACTCGCTGCTGGAAGCCTTCGTCTGCGGCGGCTTCGTCGCCCGCGACCTGGACGCCACATCCCTGCCGGTGCCG
GACGGGCTGCCGGACGACACGCCCGTCCCCGGCGGCGGGGCCGACGACCTGGCGGATATCCGCGCCTGCATCGGCCGCGC
CGCCGGCATCCTGCGTGACGCGGACGGCCTGACCCGCGCCATCCGCGCGCTGGCCCCGCATGTCATGGATGACGACCACG
CCCTGGTCGGCGCGCTGCTGTGCGTGTCCGCGCTCGGCCGGCGGGAAAGCCGGGGCGGCCATTACCGGTCCGACTATCCG
GACACGGCGCCCCAGGCGGTGCATACCCGCCTGACGATGCGCCAGGGCCTGGCCGCGCTCGCATCGCTCGCGCCTGCCGC
GGCCTCTCTTTCTGACAATGGAATCCTCGTGCCATGA

Upstream 100 bases:

>100_bases
CGATCCGCCGGGCCCTGGAAACCATGACGCACGAGGTGACGATTCCCCCGCATCTGGTGGCGGGCGGGCGTCGCGCCGTG
GAACGGATGCTCGCCGCGTC

Downstream 100 bases:

>100_bases
CCCCCTTGCCCGATCTCATGCTCGAACCGCTGGTGCGTGCCGGATTGCTGGAGGACCTGGGGTGCGGCGGGGATATCACG
ACCGATGCGCTGGCGGCGCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTGPGLTDAGSWAGQPVIAGAGLAGLATALLMDRPCVVLSPAPLATDAASALAQGGIAAAIGPDDTPALHARDTLEAGVG
LCDPVVVDAITRAGPGAIAALRDLGVAFDTGPDGRLDLHLEAAHSRPRIAHAHGDGTGAEIMRALVARTRATPRITVLDG
AALRRVIVRDGRVAGVRIARAGQELILPTSACVIATGGVGALFPTTTSPPGGLGSGLACAARAGAVLRDLEFVQFHPTAL
AVGSDAAPRVLISEAVRGAGAILVDESGARFTDELAPRDIVARAIQAHLSAGHRVLLDARAATQGHFARHFPAIARACVQ
HGIDPDRQPIPVRPAVHYHMGGIETDLRGRSTVPGLWACGEAASTGLHGANRLASNSLLEAFVCGGFVARDLDATSLPVP
DGLPDDTPVPGGGADDLADIRACIGRAAGILRDADGLTRAIRALAPHVMDDDHALVGALLCVSALGRRESRGGHYRSDYP
DTAPQAVHTRLTMRQGLAALASLAPAAASLSDNGILVP

Sequences:

>Translated_518_residues
MTGPGLTDAGSWAGQPVIAGAGLAGLATALLMDRPCVVLSPAPLATDAASALAQGGIAAAIGPDDTPALHARDTLEAGVG
LCDPVVVDAITRAGPGAIAALRDLGVAFDTGPDGRLDLHLEAAHSRPRIAHAHGDGTGAEIMRALVARTRATPRITVLDG
AALRRVIVRDGRVAGVRIARAGQELILPTSACVIATGGVGALFPTTTSPPGGLGSGLACAARAGAVLRDLEFVQFHPTAL
AVGSDAAPRVLISEAVRGAGAILVDESGARFTDELAPRDIVARAIQAHLSAGHRVLLDARAATQGHFARHFPAIARACVQ
HGIDPDRQPIPVRPAVHYHMGGIETDLRGRSTVPGLWACGEAASTGLHGANRLASNSLLEAFVCGGFVARDLDATSLPVP
DGLPDDTPVPGGGADDLADIRACIGRAAGILRDADGLTRAIRALAPHVMDDDHALVGALLCVSALGRRESRGGHYRSDYP
DTAPQAVHTRLTMRQGLAALASLAPAAASLSDNGILVP
>Mature_517_residues
TGPGLTDAGSWAGQPVIAGAGLAGLATALLMDRPCVVLSPAPLATDAASALAQGGIAAAIGPDDTPALHARDTLEAGVGL
CDPVVVDAITRAGPGAIAALRDLGVAFDTGPDGRLDLHLEAAHSRPRIAHAHGDGTGAEIMRALVARTRATPRITVLDGA
ALRRVIVRDGRVAGVRIARAGQELILPTSACVIATGGVGALFPTTTSPPGGLGSGLACAARAGAVLRDLEFVQFHPTALA
VGSDAAPRVLISEAVRGAGAILVDESGARFTDELAPRDIVARAIQAHLSAGHRVLLDARAATQGHFARHFPAIARACVQH
GIDPDRQPIPVRPAVHYHMGGIETDLRGRSTVPGLWACGEAASTGLHGANRLASNSLLEAFVCGGFVARDLDATSLPVPD
GLPDDTPVPGGGADDLADIRACIGRAAGILRDADGLTRAIRALAPHVMDDDHALVGALLCVSALGRRESRGGHYRSDYPD
TAPQAVHTRLTMRQGLAALASLAPAAASLSDNGILVP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=510, Percent_Identity=29.4117647058824, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1788928, Length=517, Percent_Identity=36.7504835589942, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1790597, Length=501, Percent_Identity=31.7365269461078, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1786942, Length=539, Percent_Identity=27.2727272727273, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17550100, Length=515, Percent_Identity=29.9029126213592, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=516, Percent_Identity=28.4883720930233, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71986328, Length=453, Percent_Identity=27.8145695364238, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6322701, Length=546, Percent_Identity=31.1355311355311, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322416, Length=520, Percent_Identity=31.1538461538462, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6322511, Length=479, Percent_Identity=26.5135699373695, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320788, Length=462, Percent_Identity=25.5411255411255, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137288, Length=522, Percent_Identity=30.2681992337165, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24655642, Length=522, Percent_Identity=30.2681992337165, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24655647, Length=522, Percent_Identity=30.2681992337165, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24663005, Length=518, Percent_Identity=26.4478764478764, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 52578; Mature: 52447

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPGLTDAGSWAGQPVIAGAGLAGLATALLMDRPCVVLSPAPLATDAASALAQGGIAAA
CCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCEEEE
IGPDDTPALHARDTLEAGVGLCDPVVVDAITRAGPGAIAALRDLGVAFDTGPDGRLDLHL
ECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCEEEEEE
EAAHSRPRIAHAHGDGTGAEIMRALVARTRATPRITVLDGAALRRVIVRDGRVAGVRIAR
ECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCEEEEEEEC
AGQELILPTSACVIATGGVGALFPTTTSPPGGLGSGLACAARAGAVLRDLEFVQFHPTAL
CCCEEEECCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCEE
AVGSDAAPRVLISEAVRGAGAILVDESGARFTDELAPRDIVARAIQAHLSAGHRVLLDAR
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECC
AATQGHFARHFPAIARACVQHGIDPDRQPIPVRPAVHYHMGGIETDLRGRSTVPGLWACG
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEECC
EAASTGLHGANRLASNSLLEAFVCGGFVARDLDATSLPVPDGLPDDTPVPGGGADDLADI
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RACIGRAAGILRDADGLTRAIRALAPHVMDDDHALVGALLCVSALGRRESRGGHYRSDYP
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DTAPQAVHTRLTMRQGLAALASLAPAAASLSDNGILVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TGPGLTDAGSWAGQPVIAGAGLAGLATALLMDRPCVVLSPAPLATDAASALAQGGIAAA
CCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCEEEE
IGPDDTPALHARDTLEAGVGLCDPVVVDAITRAGPGAIAALRDLGVAFDTGPDGRLDLHL
ECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCEEEEEE
EAAHSRPRIAHAHGDGTGAEIMRALVARTRATPRITVLDGAALRRVIVRDGRVAGVRIAR
ECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCEEEEEEEC
AGQELILPTSACVIATGGVGALFPTTTSPPGGLGSGLACAARAGAVLRDLEFVQFHPTAL
CCCEEEECCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCEE
AVGSDAAPRVLISEAVRGAGAILVDESGARFTDELAPRDIVARAIQAHLSAGHRVLLDAR
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECC
AATQGHFARHFPAIARACVQHGIDPDRQPIPVRPAVHYHMGGIETDLRGRSTVPGLWACG
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEECC
EAASTGLHGANRLASNSLLEAFVCGGFVARDLDATSLPVPDGLPDDTPVPGGGADDLADI
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RACIGRAAGILRDADGLTRAIRALAPHVMDDDHALVGALLCVSALGRRESRGGHYRSDYP
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DTAPQAVHTRLTMRQGLAALASLAPAAASLSDNGILVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]