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The map label for this gene is yfjP [H]

Identifier: 162146346

GI number: 162146346

Start: 515075

End: 515767

Strand: Direct

Name: yfjP [H]

Synonym: GDI_0521

Alternate gene names: 162146346

Gene position: 515075-515767 (Clockwise)

Preceding gene: 162146342

Following gene: 162146349

Centisome position: 13.06

GC content: 71.0

Gene sequence:

>693_bases
ATGTCATCCGTCGTTCCGGTCTCCGAACCGATCTCCGCCGTGTCCCCCGAACTGGACCGCGCCATTCGTGCCCACCTGTC
CGCCGCCGATCCCGATCTGGCGGCCCTGGTGGCCCGGATCGGGCCGTGCGGCCTGCGCATGCATGCGGCGCGCGAGCCGT
ACGAGGCGCTGGTGGACGCCATCGCGCATCAGCAGCTTCATGGGCGGGCGGCGCAGGCCATTCTGGGGCGGATGGTGGCG
CTGGCCGGCGGCGTGTTCCCCGATCCGGCGGCGTTGCTGGCGTTGCCGGTCGAAGCCCTGCGCGGCTGCGGGTTCTCGGG
GTCCAAGATCGTGGCGATGCATGGCGTCGCCAAGGCGCGGCTGGACGGGCTGGTGCCCTCGCGCCGCCAGGCGGAATCGC
TGTCAGACGCCGAACTGATCGCCCGCCTGGTCAGCCTGCGCGGCATCGGGCGCTGGACGGTCGAAATGCTGTTGATCTTC
TCGCTGGGCCGGCTGGACATCATGCCGGTGGACGATTTCGGCGTGCGCGAGGGGTGGCGATTGCTGAAGGGGCTGGACGC
CCAGCCCCGACCGAAGCAACTGGCTGCTATTGCGGCGGACTGGAGCCCCTATCGGTCGGCGGCGGCGTGGTATCTGTGGC
GCGCGGCGGATGCGGTGAAGCCGCCGGACCGCGCCAACCCCATGACCGCGTAG

Upstream 100 bases:

>100_bases
TCGGGACCCTACCCTGTGGCGATCCCGTTGTCATGTTGCCACCCTGTCATGTTGCCACCGACAGGATTGTGTTGCCACAA
GAACTCTGTTGCCATGGGGC

Downstream 100 bases:

>100_bases
CGTCAATATTGTTCGGTGAAGGCTTCCAGCAGCGCGGCGAAGCGGGTCGGGTTTTCGACATGCAGCCAGTGGCCGGCCTC
TTCGATCCGTTCCAGCCAGT

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSSVVPVSEPISAVSPELDRAIRAHLSAADPDLAALVARIGPCGLRMHAAREPYEALVDAIAHQQLHGRAAQAILGRMVA
LAGGVFPDPAALLALPVEALRGCGFSGSKIVAMHGVAKARLDGLVPSRRQAESLSDAELIARLVSLRGIGRWTVEMLLIF
SLGRLDIMPVDDFGVREGWRLLKGLDAQPRPKQLAAIAADWSPYRSAAAWYLWRAADAVKPPDRANPMTA

Sequences:

>Translated_230_residues
MSSVVPVSEPISAVSPELDRAIRAHLSAADPDLAALVARIGPCGLRMHAAREPYEALVDAIAHQQLHGRAAQAILGRMVA
LAGGVFPDPAALLALPVEALRGCGFSGSKIVAMHGVAKARLDGLVPSRRQAESLSDAELIARLVSLRGIGRWTVEMLLIF
SLGRLDIMPVDDFGVREGWRLLKGLDAQPRPKQLAAIAADWSPYRSAAAWYLWRAADAVKPPDRANPMTA
>Mature_229_residues
SSVVPVSEPISAVSPELDRAIRAHLSAADPDLAALVARIGPCGLRMHAAREPYEALVDAIAHQQLHGRAAQAILGRMVAL
AGGVFPDPAALLALPVEALRGCGFSGSKIVAMHGVAKARLDGLVPSRRQAESLSDAELIARLVSLRGIGRWTVEMLLIFS
LGRLDIMPVDDFGVREGWRLLKGLDAQPRPKQLAAIAADWSPYRSAAAWYLWRAADAVKPPDRANPMTA

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320990, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 24557; Mature: 24426

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVPVSEPISAVSPELDRAIRAHLSAADPDLAALVARIGPCGLRMHAAREPYEALVDA
CCCCCCCCCCHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
IAHQQLHGRAAQAILGRMVALAGGVFPDPAALLALPVEALRGCGFSGSKIVAMHGVAKAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHH
LDGLVPSRRQAESLSDAELIARLVSLRGIGRWTVEMLLIFSLGRLDIMPVDDFGVREGWR
HHCCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
LLKGLDAQPRPKQLAAIAADWSPYRSAAAWYLWRAADAVKPPDRANPMTA
HHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSVVPVSEPISAVSPELDRAIRAHLSAADPDLAALVARIGPCGLRMHAAREPYEALVDA
CCCCCCCCCHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
IAHQQLHGRAAQAILGRMVALAGGVFPDPAALLALPVEALRGCGFSGSKIVAMHGVAKAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHH
LDGLVPSRRQAESLSDAELIARLVSLRGIGRWTVEMLLIFSLGRLDIMPVDDFGVREGWR
HHCCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
LLKGLDAQPRPKQLAAIAADWSPYRSAAAWYLWRAADAVKPPDRANPMTA
HHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]