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The map label for this gene is 162146029

Identifier: 162146029

GI number: 162146029

Start: 211606

End: 213219

Strand: Reverse

Name: 162146029

Synonym: GDI_0197

Alternate gene names: NA

Gene position: 213219-211606 (Counterclockwise)

Preceding gene: 162146030

Following gene: 162146017

Centisome position: 5.41

GC content: 66.67

Gene sequence:

>1614_bases
ATGAACGCGCGGAACCAGATCGATCTCCAGATCATGTGGAACCGCCTGATCGCCGTCGTCGGCGAGCAGGCTCAGGTCCT
GATTCGCACGGCCTTCAGCCCGATCGTGCGCGAATGCGAGGATATTTCGGCGGGAATCTTCGACCTGCGGGGCCGCATGA
TCGCGCAGGCCGTGACCGGCACCCCCGGCCACGTCAATTCGATGGCGGAATCCGTGGGCCATTTCCTGCGCCACTTCCCC
ATCGAGACCATGAAGGACGGCGATGCCTACATCACCAACGACCCGTGGATGGGCACGGGGCACCTGAACGATTTCGTCGT
CACGACCCCGGCCTTCCACAGGGGAAAGCTTGTGGGGCTGTTCTCCTGCACCAGCCACCTGATGGATATCGGCGGCCTGG
GCTGCGGCCCGGAAGGGACGGACGTGCTGATGGAGGGGCTGTCGATCCCCATGCTCAAGCTGATCGACCAGGGCCGGGTG
AACGAGACCCTGATGGCGATGATCCGCACCAACACCCGCCTGCCGGTCGACACCGAGGGCGATACCTATTCCCTGGCGGC
CTGCAACGACGTCGGCGTGCGCGCGCTGCGCGCGATGATGGACGAATTCGCCATCGATACGCTGGAACCGCTGGCCGATT
TCATCATCGAGCGATCGTACGATGCCGTCGTGGAAAAAATCGCCGCCCTGCCACGCGGCACATGGTCGTACGACATGACG
ATAGACGGCTACGATGCGCCGCTGACGCTGCGGGCGAGCCTGACGATCGATGCCGATGGCGTGCAGGTCGATTATGACGG
GTCGGACCCCGTCGTGCCACGCGGAATCAACGTACCGCTGGCCTATACCAAGGCCTATACGGTGTTCGGCCTGGCCTGCG
CCATCGCGCCGGACGTGCCCAACAATGCCGGATCACTGGCGGCCTATCGCGTCACGGCACCGGCGGGAAGCGTGCTCAAC
GCGCCGTACCCGGCGCCGGTCTCCGCACGGCATATCGTCGGGCAGATGCTGCCGGACATGGTGTTCGGCTGCCTGCGCAC
GCCCCTGCCCGACCGGATTCCGGCCGAAGGCACATCCTGCCTGTGGAACATCACGGTACGCGGAAACTGGGTGCCGGACG
GCCAGGATACGACGCAGGCCTTCGCCCTGGCCATCACGACCAATGGCGGCACGGGCGCGCGGCCGGGCCTGGACGGGCTG
TCGGCCACCGCGTTTCCCTCCGGCGTCCATGGCACCCCGATCGAGATCGCGGAAATACAGTGCCCCCTCATCTTCTGGCG
CAAGGAATTCCGCGAAGGCAGCGGCGGCGACGGCGAGACCCGCGGCGGCCTGGGTCAGATCATGGAGATCGGCAGCACGG
AACAGCGGCCGTTCGACCTGCTGGCCGCATTCGACCGGATCACATACCCCCCGCGCGGCGCAGTCGGGGGCAAGGACGGC
GCCCCCGGCTGCGTCAAGCTGGCGGGCGGCAGGGAACTCGCAGGCAAGGGGCTACAGATTATCCCGCCCGACGAGACGTT
GATCATCATGACGCCCGGCGGGGGCGGCCTTGGCGCGCCCGGGGCCCGGACGGAAACACGACGCCAGGCGGACCGCGAGG
GCGGTTATGTATAG

Upstream 100 bases:

>100_bases
TGATCGCCGAGCGCGAGACCACCACCTACGTTTCGGCCCGCTTCGACGCCTTTATCGACGGCAGCGGCAATATCGTCATG
GACATGAAGGAAACGGCCCA

Downstream 100 bases:

>100_bases
CATACGACGGTACTGGCAGGCGGTCGAAAGACCGCCTGCCCCCGAGGCAGCCAGTGCCGGTTCACAGACATGACGGAACG
GACGCATCGGCCGATGAGTC

Product: 5-oxoprolinase (ATP-hydrolysing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MNARNQIDLQIMWNRLIAVVGEQAQVLIRTAFSPIVRECEDISAGIFDLRGRMIAQAVTGTPGHVNSMAESVGHFLRHFP
IETMKDGDAYITNDPWMGTGHLNDFVVTTPAFHRGKLVGLFSCTSHLMDIGGLGCGPEGTDVLMEGLSIPMLKLIDQGRV
NETLMAMIRTNTRLPVDTEGDTYSLAACNDVGVRALRAMMDEFAIDTLEPLADFIIERSYDAVVEKIAALPRGTWSYDMT
IDGYDAPLTLRASLTIDADGVQVDYDGSDPVVPRGINVPLAYTKAYTVFGLACAIAPDVPNNAGSLAAYRVTAPAGSVLN
APYPAPVSARHIVGQMLPDMVFGCLRTPLPDRIPAEGTSCLWNITVRGNWVPDGQDTTQAFALAITTNGGTGARPGLDGL
SATAFPSGVHGTPIEIAEIQCPLIFWRKEFREGSGGDGETRGGLGQIMEIGSTEQRPFDLLAAFDRITYPPRGAVGGKDG
APGCVKLAGGRELAGKGLQIIPPDETLIIMTPGGGGLGAPGARTETRRQADREGGYV

Sequences:

>Translated_537_residues
MNARNQIDLQIMWNRLIAVVGEQAQVLIRTAFSPIVRECEDISAGIFDLRGRMIAQAVTGTPGHVNSMAESVGHFLRHFP
IETMKDGDAYITNDPWMGTGHLNDFVVTTPAFHRGKLVGLFSCTSHLMDIGGLGCGPEGTDVLMEGLSIPMLKLIDQGRV
NETLMAMIRTNTRLPVDTEGDTYSLAACNDVGVRALRAMMDEFAIDTLEPLADFIIERSYDAVVEKIAALPRGTWSYDMT
IDGYDAPLTLRASLTIDADGVQVDYDGSDPVVPRGINVPLAYTKAYTVFGLACAIAPDVPNNAGSLAAYRVTAPAGSVLN
APYPAPVSARHIVGQMLPDMVFGCLRTPLPDRIPAEGTSCLWNITVRGNWVPDGQDTTQAFALAITTNGGTGARPGLDGL
SATAFPSGVHGTPIEIAEIQCPLIFWRKEFREGSGGDGETRGGLGQIMEIGSTEQRPFDLLAAFDRITYPPRGAVGGKDG
APGCVKLAGGRELAGKGLQIIPPDETLIIMTPGGGGLGAPGARTETRRQADREGGYV
>Mature_537_residues
MNARNQIDLQIMWNRLIAVVGEQAQVLIRTAFSPIVRECEDISAGIFDLRGRMIAQAVTGTPGHVNSMAESVGHFLRHFP
IETMKDGDAYITNDPWMGTGHLNDFVVTTPAFHRGKLVGLFSCTSHLMDIGGLGCGPEGTDVLMEGLSIPMLKLIDQGRV
NETLMAMIRTNTRLPVDTEGDTYSLAACNDVGVRALRAMMDEFAIDTLEPLADFIIERSYDAVVEKIAALPRGTWSYDMT
IDGYDAPLTLRASLTIDADGVQVDYDGSDPVVPRGINVPLAYTKAYTVFGLACAIAPDVPNNAGSLAAYRVTAPAGSVLN
APYPAPVSARHIVGQMLPDMVFGCLRTPLPDRIPAEGTSCLWNITVRGNWVPDGQDTTQAFALAITTNGGTGARPGLDGL
SATAFPSGVHGTPIEIAEIQCPLIFWRKEFREGSGGDGETRGGLGQIMEIGSTEQRPFDLLAAFDRITYPPRGAVGGKDG
APGCVKLAGGRELAGKGLQIIPPDETLIIMTPGGGGLGAPGARTETRRQADREGGYV

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=549, Percent_Identity=29.6903460837887, Blast_Score=149, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI133901900, Length=539, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI133901902, Length=509, Percent_Identity=28.2907662082515, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322634, Length=546, Percent_Identity=25.6410256410256, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI45550492, Length=541, Percent_Identity=26.9870609981516, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 57171; Mature: 57171

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARNQIDLQIMWNRLIAVVGEQAQVLIRTAFSPIVRECEDISAGIFDLRGRMIAQAVTG
CCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
TPGHVNSMAESVGHFLRHFPIETMKDGDAYITNDPWMGTGHLNDFVVTTPAFHRGKLVGL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCEEEH
FSCTSHLMDIGGLGCGPEGTDVLMEGLSIPMLKLIDQGRVNETLMAMIRTNTRLPVDTEG
HHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
DTYSLAACNDVGVRALRAMMDEFAIDTLEPLADFIIERSYDAVVEKIAALPRGTWSYDMT
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEE
IDGYDAPLTLRASLTIDADGVQVDYDGSDPVVPRGINVPLAYTKAYTVFGLACAIAPDVP
ECCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHEEECCCCC
NNAGSLAAYRVTAPAGSVLNAPYPAPVSARHIVGQMLPDMVFGCLRTPLPDRIPAEGTSC
CCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
LWNITVRGNWVPDGQDTTQAFALAITTNGGTGARPGLDGLSATAFPSGVHGTPIEIAEIQ
EEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
CPLIFWRKEFREGSGGDGETRGGLGQIMEIGSTEQRPFDLLAAFDRITYPPRGAVGGKDG
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
APGCVKLAGGRELAGKGLQIIPPDETLIIMTPGGGGLGAPGARTETRRQADREGGYV
CCCEEEECCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MNARNQIDLQIMWNRLIAVVGEQAQVLIRTAFSPIVRECEDISAGIFDLRGRMIAQAVTG
CCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
TPGHVNSMAESVGHFLRHFPIETMKDGDAYITNDPWMGTGHLNDFVVTTPAFHRGKLVGL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCEEEH
FSCTSHLMDIGGLGCGPEGTDVLMEGLSIPMLKLIDQGRVNETLMAMIRTNTRLPVDTEG
HHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
DTYSLAACNDVGVRALRAMMDEFAIDTLEPLADFIIERSYDAVVEKIAALPRGTWSYDMT
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEE
IDGYDAPLTLRASLTIDADGVQVDYDGSDPVVPRGINVPLAYTKAYTVFGLACAIAPDVP
ECCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHEEECCCCC
NNAGSLAAYRVTAPAGSVLNAPYPAPVSARHIVGQMLPDMVFGCLRTPLPDRIPAEGTSC
CCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
LWNITVRGNWVPDGQDTTQAFALAITTNGGTGARPGLDGLSATAFPSGVHGTPIEIAEIQ
EEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
CPLIFWRKEFREGSGGDGETRGGLGQIMEIGSTEQRPFDLLAAFDRITYPPRGAVGGKDG
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
APGCVKLAGGRELAGKGLQIIPPDETLIIMTPGGGGLGAPGARTETRRQADREGGYV
CCCEEEECCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]