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The map label for this gene is 162146030

Identifier: 162146030

GI number: 162146030

Start: 213216

End: 215309

Strand: Reverse

Name: 162146030

Synonym: GDI_0198

Alternate gene names: NA

Gene position: 215309-213216 (Counterclockwise)

Preceding gene: 162146032

Following gene: 162146029

Centisome position: 5.46

GC content: 69.2

Gene sequence:

>2094_bases
ATGTCCCCCAAGATGCAAATTCGCCTGGCCGCCGATATTGGCGGAACGTTCACGGATATCGTGCTGGAAACCCCGCGCGG
GCGCCTGACGCGCAAGGTCCTGACGACGCCGCATGCGCCCGAGGAAGGGGTGATGACCGGAATCGGCCTGATCCTGGGCG
ATGCCGGGCTGGCCTTCGCCGACGTCACGGTCTTCGTCCATGGCACGACGCTGGCGACCAACGCCATTATCGAACGGCGA
GGCGCCCTGACGGTACTGATCGGCACCGAGGGATTTCGCGACATCCTGGAAATCGGCACGGAAAGCCGGTTCAACCAGTA
TGACATCATGATCCGGAAGCCGGCGCCGCTGGTTCCCCGTACCGCGCGCTTCACCGTGCCGGAGCGCATGGACGCACGGG
GGCGGGTCCAGAGGCCGCTGGACGAGGACGCGCTGCGCGCGCTGGCGCCGCGCCTGCGCGCCGCGGGGGCCGAGGCGATC
GCGATCGCCTTCATGCATTCCTACGCGAATCCGGACCATGAGCGCCGCGCCGGCGAGATCCTGGCCGAGGCGCTTCCGGA
CATTCCGATTTCGCTTTCCAGCGCCGTCTGCCCCGAAGTGCGTGAATATGAGCGCACCTCCACCACCGTCGCCAACGCCT
ATGTCCAGCCGCTGATGGCCGGCTATCTGGGCCGGCTGCGACAGGCGCTGGATGCGCAGTCGTTCCGTGGGGCGCTGTAT
CTGGTGACGTCGGGCGGCGGACTGACGTCGCTGGAGACGGCGCGGCAGTTTCCGGTGCGCCTGGTCGAATCCGGTCCGGC
GGGCGGCGCGATCTTCGCCGCCCAATGCGCCGGGCGCCTGGCCGAGGATCGTGTCCTGTCCTTCGACATGGGCGGCACCA
CGGCCAAGATCTGCCTGATCGAACACGGTCAGCCCGCATCCTCGCGCGTGTTCGAAGTGGATCGCACGGCCCGTTTCATG
AAAGGATCGGGCCTGCCGCTGCGCATTCCCGTCATCGAAATGGTCGAGATCGGCGCGGGGGGCGGGTCGCTCGTCCATCT
GGACGCCATGAAGCGCGTCGTCGTCGGGCCGGAAAGCGCATCGTCCGTACCCGGGCCGGCCTGCTACGGGCTGGGGGGCA
CGCGCCCGGCCGTGACCGACGCGGACGTGACGCTGGGCCTGATCCAGCCGGAGGCCTTCGCCGGCGGCAGCATGACCCTG
CAGCCCGAGCGCGCGGCGATCGCGTTGCAGCAGGCCGTCGGCACCACGCTGGGCCTGTCGCCGGAAATGGCGGCCCACGC
GGTCTATGAAATCGTGTGCGAGAACATGGCCAGCGCCGCCCGCGTGCATGCCGCCGAACGTGGGGCGGTCATCGGTACCC
ACACCATGATCGCCTTCGGCGGCGCGGCCCCGCTGCATGCCGCCCGCGTGGCCGAAAAACTGGGGGTCCGCCGGCTGGTC
GTGCCGTCGAACGCCGGTGTGGGGTCCGCCGTCGGCTTCCTGGCCGCCCCGGTCTCGTTCGAGATCGTCCGCTCCTGCCC
GGTGCGGCTGAATGCCGGCTTCGACACCGCCGCCATGACGTCCCTGCTGGACGATATGACGGACACGGCGCGGGACATGG
TGCAGGACGGAGCCGACGCGGCGGCGCTGACCGTCCGGCGCGGCGGCTTCATGCGCTATGTCGGACAGGGACACGAAATC
ATCGTGTCCCTGCCGGACGGACCGCTGACGCCCGACGCCCTGCCGGCGCTGCGGGCGGCATTCGAGGCGGAATATGCCCG
CCAGTTCGAGCGCGTCATCCCCAACGCCCCGGTCGAGATCCTGAACTGGTCGGTCTGTGTTTCGACCACGCCCGCGAAAC
CCGAACCCTTCGCCCCCACGCCGGCACGGCACGGGGCGGCGCCGGAACGGACGCGCCGGCTGTTCGACGGGGCCAGGGGG
CAGTTCGTAGAACTGGCGGTCCATGACCGCGACGACATGCGCCCCGGCAGCATCGTTCCCGGACCGGCCCTGATCGCCGA
GCGCGAGACCACCACCTACGTTTCGGCCCGCTTCGACGCCTTTATCGACGGCAGCGGCAATATCGTCATGGACATGAAGG
AAACGGCCCAATGA

Upstream 100 bases:

>100_bases
GGCCGGGTTTCGTGTTCGTCATGAGGCATCTCGCTCCCGTCTATTCGGCGCCGGACCGTGGCGGCGCAGCCTGATCCTAA
CCTGAACCCGGAGGCAGGGC

Downstream 100 bases:

>100_bases
ACGCGCGGAACCAGATCGATCTCCAGATCATGTGGAACCGCCTGATCGCCGTCGTCGGCGAGCAGGCTCAGGTCCTGATT
CGCACGGCCTTCAGCCCGAT

Product: 5-oxoprolinase (ATP-hydrolysing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSPKMQIRLAADIGGTFTDIVLETPRGRLTRKVLTTPHAPEEGVMTGIGLILGDAGLAFADVTVFVHGTTLATNAIIERR
GALTVLIGTEGFRDILEIGTESRFNQYDIMIRKPAPLVPRTARFTVPERMDARGRVQRPLDEDALRALAPRLRAAGAEAI
AIAFMHSYANPDHERRAGEILAEALPDIPISLSSAVCPEVREYERTSTTVANAYVQPLMAGYLGRLRQALDAQSFRGALY
LVTSGGGLTSLETARQFPVRLVESGPAGGAIFAAQCAGRLAEDRVLSFDMGGTTAKICLIEHGQPASSRVFEVDRTARFM
KGSGLPLRIPVIEMVEIGAGGGSLVHLDAMKRVVVGPESASSVPGPACYGLGGTRPAVTDADVTLGLIQPEAFAGGSMTL
QPERAAIALQQAVGTTLGLSPEMAAHAVYEIVCENMASAARVHAAERGAVIGTHTMIAFGGAAPLHAARVAEKLGVRRLV
VPSNAGVGSAVGFLAAPVSFEIVRSCPVRLNAGFDTAAMTSLLDDMTDTARDMVQDGADAAALTVRRGGFMRYVGQGHEI
IVSLPDGPLTPDALPALRAAFEAEYARQFERVIPNAPVEILNWSVCVSTTPAKPEPFAPTPARHGAAPERTRRLFDGARG
QFVELAVHDRDDMRPGSIVPGPALIAERETTTYVSARFDAFIDGSGNIVMDMKETAQ

Sequences:

>Translated_697_residues
MSPKMQIRLAADIGGTFTDIVLETPRGRLTRKVLTTPHAPEEGVMTGIGLILGDAGLAFADVTVFVHGTTLATNAIIERR
GALTVLIGTEGFRDILEIGTESRFNQYDIMIRKPAPLVPRTARFTVPERMDARGRVQRPLDEDALRALAPRLRAAGAEAI
AIAFMHSYANPDHERRAGEILAEALPDIPISLSSAVCPEVREYERTSTTVANAYVQPLMAGYLGRLRQALDAQSFRGALY
LVTSGGGLTSLETARQFPVRLVESGPAGGAIFAAQCAGRLAEDRVLSFDMGGTTAKICLIEHGQPASSRVFEVDRTARFM
KGSGLPLRIPVIEMVEIGAGGGSLVHLDAMKRVVVGPESASSVPGPACYGLGGTRPAVTDADVTLGLIQPEAFAGGSMTL
QPERAAIALQQAVGTTLGLSPEMAAHAVYEIVCENMASAARVHAAERGAVIGTHTMIAFGGAAPLHAARVAEKLGVRRLV
VPSNAGVGSAVGFLAAPVSFEIVRSCPVRLNAGFDTAAMTSLLDDMTDTARDMVQDGADAAALTVRRGGFMRYVGQGHEI
IVSLPDGPLTPDALPALRAAFEAEYARQFERVIPNAPVEILNWSVCVSTTPAKPEPFAPTPARHGAAPERTRRLFDGARG
QFVELAVHDRDDMRPGSIVPGPALIAERETTTYVSARFDAFIDGSGNIVMDMKETAQ
>Mature_696_residues
SPKMQIRLAADIGGTFTDIVLETPRGRLTRKVLTTPHAPEEGVMTGIGLILGDAGLAFADVTVFVHGTTLATNAIIERRG
ALTVLIGTEGFRDILEIGTESRFNQYDIMIRKPAPLVPRTARFTVPERMDARGRVQRPLDEDALRALAPRLRAAGAEAIA
IAFMHSYANPDHERRAGEILAEALPDIPISLSSAVCPEVREYERTSTTVANAYVQPLMAGYLGRLRQALDAQSFRGALYL
VTSGGGLTSLETARQFPVRLVESGPAGGAIFAAQCAGRLAEDRVLSFDMGGTTAKICLIEHGQPASSRVFEVDRTARFMK
GSGLPLRIPVIEMVEIGAGGGSLVHLDAMKRVVVGPESASSVPGPACYGLGGTRPAVTDADVTLGLIQPEAFAGGSMTLQ
PERAAIALQQAVGTTLGLSPEMAAHAVYEIVCENMASAARVHAAERGAVIGTHTMIAFGGAAPLHAARVAEKLGVRRLVV
PSNAGVGSAVGFLAAPVSFEIVRSCPVRLNAGFDTAAMTSLLDDMTDTARDMVQDGADAAALTVRRGGFMRYVGQGHEII
VSLPDGPLTPDALPALRAAFEAEYARQFERVIPNAPVEILNWSVCVSTTPAKPEPFAPTPARHGAAPERTRRLFDGARGQ
FVELAVHDRDDMRPGSIVPGPALIAERETTTYVSARFDAFIDGSGNIVMDMKETAQ

Specific function: Unknown

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=748, Percent_Identity=29.8128342245989, Blast_Score=201, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI133901900, Length=400, Percent_Identity=30.75, Blast_Score=149, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322634, Length=749, Percent_Identity=26.0347129506008, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550492, Length=738, Percent_Identity=29.1327913279133, Blast_Score=224, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 73824; Mature: 73692

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKMQIRLAADIGGTFTDIVLETPRGRLTRKVLTTPHAPEEGVMTGIGLILGDAGLAFA
CCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEE
DVTVFVHGTTLATNAIIERRGALTVLIGTEGFRDILEIGTESRFNQYDIMIRKPAPLVPR
EEEEEEECCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCC
TARFTVPERMDARGRVQRPLDEDALRALAPRLRAAGAEAIAIAFMHSYANPDHERRAGEI
CEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
LAEALPDIPISLSSAVCPEVREYERTSTTVANAYVQPLMAGYLGRLRQALDAQSFRGALY
HHHHCCCCCEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEE
LVTSGGGLTSLETARQFPVRLVESGPAGGAIFAAQCAGRLAEDRVLSFDMGGTTAKICLI
EEECCCCCCHHHHHHHCCEEEEECCCCCCEEEHHHHHCCHHHCCEEEEECCCCEEEEEEE
EHGQPASSRVFEVDRTARFMKGSGLPLRIPVIEMVEIGAGGGSLVHLDAMKRVVVGPESA
ECCCCCCCCEEEHHHHHHHHCCCCCEEEECCEEEEEECCCCCCEEEHHHHHHEEECCCCC
SSVPGPACYGLGGTRPAVTDADVTLGLIQPEAFAGGSMTLQPERAAIALQQAVGTTLGLS
CCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCCCC
PEMAAHAVYEIVCENMASAARVHAAERGAVIGTHTMIAFGGAAPLHAARVAEKLGVRRLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHCCEEEE
VPSNAGVGSAVGFLAAPVSFEIVRSCPVRLNAGFDTAAMTSLLDDMTDTARDMVQDGADA
ECCCCCCCHHHHHHHCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AALTVRRGGFMRYVGQGHEIIVSLPDGPLTPDALPALRAAFEAEYARQFERVIPNAPVEI
EEEEEECCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LNWSVCVSTTPAKPEPFAPTPARHGAAPERTRRLFDGARGQFVELAVHDRDDMRPGSIVP
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCC
GPALIAERETTTYVSARFDAFIDGSGNIVMDMKETAQ
CCCEEECCCCCEEEEEEEEEEECCCCCEEEECHHHCC
>Mature Secondary Structure 
SPKMQIRLAADIGGTFTDIVLETPRGRLTRKVLTTPHAPEEGVMTGIGLILGDAGLAFA
CCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEE
DVTVFVHGTTLATNAIIERRGALTVLIGTEGFRDILEIGTESRFNQYDIMIRKPAPLVPR
EEEEEEECCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCC
TARFTVPERMDARGRVQRPLDEDALRALAPRLRAAGAEAIAIAFMHSYANPDHERRAGEI
CEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
LAEALPDIPISLSSAVCPEVREYERTSTTVANAYVQPLMAGYLGRLRQALDAQSFRGALY
HHHHCCCCCEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEE
LVTSGGGLTSLETARQFPVRLVESGPAGGAIFAAQCAGRLAEDRVLSFDMGGTTAKICLI
EEECCCCCCHHHHHHHCCEEEEECCCCCCEEEHHHHHCCHHHCCEEEEECCCCEEEEEEE
EHGQPASSRVFEVDRTARFMKGSGLPLRIPVIEMVEIGAGGGSLVHLDAMKRVVVGPESA
ECCCCCCCCEEEHHHHHHHHCCCCCEEEECCEEEEEECCCCCCEEEHHHHHHEEECCCCC
SSVPGPACYGLGGTRPAVTDADVTLGLIQPEAFAGGSMTLQPERAAIALQQAVGTTLGLS
CCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCCCC
PEMAAHAVYEIVCENMASAARVHAAERGAVIGTHTMIAFGGAAPLHAARVAEKLGVRRLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHCCEEEE
VPSNAGVGSAVGFLAAPVSFEIVRSCPVRLNAGFDTAAMTSLLDDMTDTARDMVQDGADA
ECCCCCCCHHHHHHHCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AALTVRRGGFMRYVGQGHEIIVSLPDGPLTPDALPALRAAFEAEYARQFERVIPNAPVEI
EEEEEECCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LNWSVCVSTTPAKPEPFAPTPARHGAAPERTRRLFDGARGQFVELAVHDRDDMRPGSIVP
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCC
GPALIAERETTTYVSARFDAFIDGSGNIVMDMKETAQ
CCCEEECCCCCEEEEEEEEEEECCCCCEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]