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The map label for this gene is yofA [H]

Identifier: 162146019

GI number: 162146019

Start: 200124

End: 201014

Strand: Direct

Name: yofA [H]

Synonym: GDI_0187

Alternate gene names: 162146019

Gene position: 200124-201014 (Clockwise)

Preceding gene: 162146018

Following gene: 162146020

Centisome position: 5.07

GC content: 64.65

Gene sequence:

>891_bases
ATGGACTTGAAGAGTCTGGAGGCCGTGCTGTGGATCGACAGGCTGGGCGGCTTCAAGGCGGCGGCCGAGGCGCTGCGCAT
GACGCAGCCCGCGATCTCGATCCGGGTGTCGCAGCTGGAGGCCATGCTGGAGACGCGGATCTTCCATCGCTCCGGCAAGC
GTGTCTTGCCCACGCCCGTCGGCGTGACGCTGATCCACTACGCCGAGCGCATCCTGCAGCTGCGGGACGAGGCACTTCTG
ACCATCAAGGGCCATAACGAGGAAAACGGTATCCTGCGCCTCGGGACCGTGGAAACGACCGCGCATACCTGGCTGGCGCA
ACTGCTCTGCCGCATCGACGAGAAATACCCCAATATCACGGTCGATCTCGATATCGGCATTTCCGACGATATCCAGCGCA
AGGTCGCGAAGGGCATGCTGGATGTCGGCCTGTTCATGGGGCCGGTCAATTCTCCGATGATGATCGAGACGCCGGTCTGC
AGCTTCGAGGTCGCGCTGCTGGCGCATCGCACGGTCGTGGGCCAGCGCGGCGGGCGCACGGATCTGGGCAGCCTGCCCGC
GCTTCCGATCATGACCTTCTCACGCAATACCGTTCCGCATTCGATGCTGCACCGCCTGCTGGGCAGGGAAGGGGGCAGCC
ACCATCGCGTTCATGCCATGAGTTCGGTACCGACGATCGTCAGCATGCTCCTCAGCGGCGCGGGACTGGCCCTGTTGCCG
CCGGATGTCGTGCGCGAACATCTGGATTCGGGTGCATTGTGCAGACTCGATGTCGGGCAGGCGCTGCCGCCTCTCAATTG
CGTCGCCGGCCGGCTGATGAAGCACAGCTCGGGCCTGGTGGAGGATGTCGTCGCCATGGCGCGGAACGTGGCGCAAAGCG
CAGGGGCCTGA

Upstream 100 bases:

>100_bases
GTCCTGTGGCCACGGGCGGCTGTGCGGAGGGAGGCTTATCTTTGGCGTGCCGACCGGTCCCTGTGATATAAACGCGCCTT
ATAGGCAGGGATCGGGTGCG

Downstream 100 bases:

>100_bases
CGCAGGTGACGCTCCGGCGACGGCCGAAGCCGCGCATGACACAAGAACCGGGAATGACACGGGAAATCGAGCGGTATCGT
GGCGGACGATACGAAGAAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDLKSLEAVLWIDRLGGFKAAAEALRMTQPAISIRVSQLEAMLETRIFHRSGKRVLPTPVGVTLIHYAERILQLRDEALL
TIKGHNEENGILRLGTVETTAHTWLAQLLCRIDEKYPNITVDLDIGISDDIQRKVAKGMLDVGLFMGPVNSPMMIETPVC
SFEVALLAHRTVVGQRGGRTDLGSLPALPIMTFSRNTVPHSMLHRLLGREGGSHHRVHAMSSVPTIVSMLLSGAGLALLP
PDVVREHLDSGALCRLDVGQALPPLNCVAGRLMKHSSGLVEDVVAMARNVAQSAGA

Sequences:

>Translated_296_residues
MDLKSLEAVLWIDRLGGFKAAAEALRMTQPAISIRVSQLEAMLETRIFHRSGKRVLPTPVGVTLIHYAERILQLRDEALL
TIKGHNEENGILRLGTVETTAHTWLAQLLCRIDEKYPNITVDLDIGISDDIQRKVAKGMLDVGLFMGPVNSPMMIETPVC
SFEVALLAHRTVVGQRGGRTDLGSLPALPIMTFSRNTVPHSMLHRLLGREGGSHHRVHAMSSVPTIVSMLLSGAGLALLP
PDVVREHLDSGALCRLDVGQALPPLNCVAGRLMKHSSGLVEDVVAMARNVAQSAGA
>Mature_296_residues
MDLKSLEAVLWIDRLGGFKAAAEALRMTQPAISIRVSQLEAMLETRIFHRSGKRVLPTPVGVTLIHYAERILQLRDEALL
TIKGHNEENGILRLGTVETTAHTWLAQLLCRIDEKYPNITVDLDIGISDDIQRKVAKGMLDVGLFMGPVNSPMMIETPVC
SFEVALLAHRTVVGQRGGRTDLGSLPALPIMTFSRNTVPHSMLHRLLGREGGSHHRVHAMSSVPTIVSMLLSGAGLALLP
PDVVREHLDSGALCRLDVGQALPPLNCVAGRLMKHSSGLVEDVVAMARNVAQSAGA

Specific function: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=26.530612244898, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788481, Length=258, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=31.875, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=170, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789639, Length=261, Percent_Identity=26.4367816091954, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788626, Length=125, Percent_Identity=33.6, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790262, Length=295, Percent_Identity=24.406779661017, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32081; Mature: 32081

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKSLEAVLWIDRLGGFKAAAEALRMTQPAISIRVSQLEAMLETRIFHRSGKRVLPTPV
CCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCH
GVTLIHYAERILQLRDEALLTIKGHNEENGILRLGTVETTAHTWLAQLLCRIDEKYPNIT
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEE
VDLDIGISDDIQRKVAKGMLDVGLFMGPVNSPMMIETPVCSFEVALLAHRTVVGQRGGRT
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCC
DLGSLPALPIMTFSRNTVPHSMLHRLLGREGGSHHRVHAMSSVPTIVSMLLSGAGLALLP
CCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEECC
PDVVREHLDSGALCRLDVGQALPPLNCVAGRLMKHSSGLVEDVVAMARNVAQSAGA
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLKSLEAVLWIDRLGGFKAAAEALRMTQPAISIRVSQLEAMLETRIFHRSGKRVLPTPV
CCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCH
GVTLIHYAERILQLRDEALLTIKGHNEENGILRLGTVETTAHTWLAQLLCRIDEKYPNIT
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEE
VDLDIGISDDIQRKVAKGMLDVGLFMGPVNSPMMIETPVCSFEVALLAHRTVVGQRGGRT
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCC
DLGSLPALPIMTFSRNTVPHSMLHRLLGREGGSHHRVHAMSSVPTIVSMLLSGAGLALLP
CCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEECC
PDVVREHLDSGALCRLDVGQALPPLNCVAGRLMKHSSGLVEDVVAMARNVAQSAGA
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA