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The map label for this gene is trmE

Identifier: 161899035

GI number: 161899035

Start: 3562131

End: 3563504

Strand: Reverse

Name: trmE

Synonym: STH3336

Alternate gene names: 161899035

Gene position: 3563504-3562131 (Counterclockwise)

Preceding gene: 51894471

Following gene: 51894469

Centisome position: 99.93

GC content: 72.63

Gene sequence:

>1374_bases
GTGGGGGAGGAGACCATCGCCGCGATCGCGACCGGAGCCGGAGAGGGCGGCATTGGCATCGTGCGGATAAGCGGGGCCGA
CGCCCTGCAGGTGGCCGAGCGGATCTTCCGCCCGCGGCGGGGCCGGCCGCTGGGTTGCCGGCGTTCGCACACGGTGACTT
ACGGCTGGGTGGTCACGCCGGGCGGTGATCGGATCGATGAGGCGCTGGCCCTGGTCATGCGGGGGCCGCACTCGTACACG
GGCGAGGACGTGGTGGAACTGCAGTGCCACGGCGGCCAGCTGGCGGTGCGCCGGGTGCTGGAGCAGGCGCTGCAGGCCGG
GGCGCGCCTCGCGGAGCCGGGAGAGTTCACCCGGCGGGCATTCCTCAACGGCCGGCTCGACCTGTCGCAGGCCGAGGCCG
TTGTCGACCTGATCCGGGCGAAGACCGACCGGGCGATGGCAGCCGCGGTCGCCCACCTGAGGGGCAGCCTGCGCCAGGCC
ATCGGGCGGATCCGGGAACGGCTGATGGAGATGATGGCGCACCTGGAGGCGGACATCGACTTCCCGGAGCTGGAGCTGGA
GGTGCAGACCCGGGAGGAAGTGGCGGCCGGCTGCGCCTGGTGTCTGGGCGAGGTGGAGCGGCTGCTCGGCGGCGCCCGCA
CCGGCCGCATCCTGCGGGAGGGGCTGCGGGCCGTGCTGGCGGGGCGGCCCAACGTGGGCAAGTCAAGCCTGCTCAACCGG
CTTGTGCGGGAGAACCGGGCGATTGTGACGCCGATCCCCGGCACGACCCGGGACGTGATCGCCGAGTGGGTCGAGCTGGG
CGGCGTGCCGGTGCAGCTCTTCGACACGGCGGGCCTCAGGCCGACGGACGATCCCGTGGAGAGGATCGGGGTGGCGCGCA
CCCATGAAGCCCTGGCCCAGGCGCATCTGGTTCTGGTGGTGGTGGACGCCGCGGCCGGGCTGGGGCCGGAGGATCGGGAG
TGGATCTCCCAGTTGCCGCAAGGCGCGGCGCGGGTCGGGGTGGCCAACAAGATCGACCTGAATCCGGCGTTCGAGCTGTC
GGCCTTGCGGGAGGCGCTGGGCGGCGCTCCGGTGGTGGGGGTCTCGGCCGAGACGGGGGAAGGGTTTGACGCGCTGGAGG
CGGAGGTCGCCCGGGTGGCGGGGGCCTTCGACGCCAGCGAGGAACTGCTGGTGAACGCCCGGCAGGCCGAAGCGATTCGG
CGTGCCAGGAATCACCTGCGGGACGCGCAGGCGACCCTGGAGAGCGGGCTAGGGGACGAGCTGGTGGCGATCGACCTGCG
TGCCGCCTGGATGGCCCTGGGGGAGGTCACCGGCGAGACGGCCGGCGAGGAGCTGCTGGACCAGATCTTCAGCCGGTTCT
GCATTGGGAAGTGA

Upstream 100 bases:

>100_bases
ATCCGTTCCGCAGGGTGGTCATCTATAAGAAGGATTAGAAGGGTGCCGGTGGCCAGGTAGCATCGCTACCCGGCCACTGT
TGTTGAAAGGAGGAAAAGCC

Downstream 100 bases:

>100_bases
GCGGGATGGGCCTGACCAAGGAGTACGACGTGATCGTGGTCGGCGCCGGGCACGCCGGAATCGAGGCGGCGCTCGCGGCC
GCACGCAAGGGCATGCGCAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MGEETIAAIATGAGEGGIGIVRISGADALQVAERIFRPRRGRPLGCRRSHTVTYGWVVTPGGDRIDEALALVMRGPHSYT
GEDVVELQCHGGQLAVRRVLEQALQAGARLAEPGEFTRRAFLNGRLDLSQAEAVVDLIRAKTDRAMAAAVAHLRGSLRQA
IGRIRERLMEMMAHLEADIDFPELELEVQTREEVAAGCAWCLGEVERLLGGARTGRILREGLRAVLAGRPNVGKSSLLNR
LVRENRAIVTPIPGTTRDVIAEWVELGGVPVQLFDTAGLRPTDDPVERIGVARTHEALAQAHLVLVVVDAAAGLGPEDRE
WISQLPQGAARVGVANKIDLNPAFELSALREALGGAPVVGVSAETGEGFDALEAEVARVAGAFDASEELLVNARQAEAIR
RARNHLRDAQATLESGLGDELVAIDLRAAWMALGEVTGETAGEELLDQIFSRFCIGK

Sequences:

>Translated_457_residues
MGEETIAAIATGAGEGGIGIVRISGADALQVAERIFRPRRGRPLGCRRSHTVTYGWVVTPGGDRIDEALALVMRGPHSYT
GEDVVELQCHGGQLAVRRVLEQALQAGARLAEPGEFTRRAFLNGRLDLSQAEAVVDLIRAKTDRAMAAAVAHLRGSLRQA
IGRIRERLMEMMAHLEADIDFPELELEVQTREEVAAGCAWCLGEVERLLGGARTGRILREGLRAVLAGRPNVGKSSLLNR
LVRENRAIVTPIPGTTRDVIAEWVELGGVPVQLFDTAGLRPTDDPVERIGVARTHEALAQAHLVLVVVDAAAGLGPEDRE
WISQLPQGAARVGVANKIDLNPAFELSALREALGGAPVVGVSAETGEGFDALEAEVARVAGAFDASEELLVNARQAEAIR
RARNHLRDAQATLESGLGDELVAIDLRAAWMALGEVTGETAGEELLDQIFSRFCIGK
>Mature_456_residues
GEETIAAIATGAGEGGIGIVRISGADALQVAERIFRPRRGRPLGCRRSHTVTYGWVVTPGGDRIDEALALVMRGPHSYTG
EDVVELQCHGGQLAVRRVLEQALQAGARLAEPGEFTRRAFLNGRLDLSQAEAVVDLIRAKTDRAMAAAVAHLRGSLRQAI
GRIRERLMEMMAHLEADIDFPELELEVQTREEVAAGCAWCLGEVERLLGGARTGRILREGLRAVLAGRPNVGKSSLLNRL
VRENRAIVTPIPGTTRDVIAEWVELGGVPVQLFDTAGLRPTDDPVERIGVARTHEALAQAHLVLVVVDAAAGLGPEDREW
ISQLPQGAARVGVANKIDLNPAFELSALREALGGAPVVGVSAETGEGFDALEAEVARVAGAFDASEELLVNARQAEAIRR
ARNHLRDAQATLESGLGDELVAIDLRAAWMALGEVTGETAGEELLDQIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=474, Percent_Identity=38.3966244725738, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=37.007874015748, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI193082993, Length=464, Percent_Identity=38.1465517241379, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=39.9141630901288, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=40.1639344262295, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=32.7548806941432, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=494, Percent_Identity=32.5910931174089, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45550813, Length=486, Percent_Identity=32.3045267489712, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SYMTH (Q67J33)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_077161.2
- HSSP:   Q9X1F8
- GeneID:   2981084
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3336
- NMPDR:   fig|292459.1.peg.3202
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   STHE292459:STH3336-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48885; Mature: 48754

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 86-86 BINDING 125-125 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEETIAAIATGAGEGGIGIVRISGADALQVAERIFRPRRGRPLGCRRSHTVTYGWVVTP
CCCHHEEHEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
GGDRIDEALALVMRGPHSYTGEDVVELQCHGGQLAVRRVLEQALQAGARLAEPGEFTRRA
CCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FLNGRLDLSQAEAVVDLIRAKTDRAMAAAVAHLRGSLRQAIGRIRERLMEMMAHLEADID
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FPELELEVQTREEVAAGCAWCLGEVERLLGGARTGRILREGLRAVLAGRPNVGKSSLLNR
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LVRENRAIVTPIPGTTRDVIAEWVELGGVPVQLFDTAGLRPTDDPVERIGVARTHEALAQ
HHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCHHHHHHHHHH
AHLVLVVVDAAAGLGPEDREWISQLPQGAARVGVANKIDLNPAFELSALREALGGAPVVG
HHHHEEEEEHHCCCCCCHHHHHHHCCCHHHHCCCCCEECCCCHHHHHHHHHHHCCCCEEE
VSAETGEGFDALEAEVARVAGAFDASEELLVNARQAEAIRRARNHLRDAQATLESGLGDE
ECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LVAIDLRAAWMALGEVTGETAGEELLDQIFSRFCIGK
EEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GEETIAAIATGAGEGGIGIVRISGADALQVAERIFRPRRGRPLGCRRSHTVTYGWVVTP
CCHHEEHEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
GGDRIDEALALVMRGPHSYTGEDVVELQCHGGQLAVRRVLEQALQAGARLAEPGEFTRRA
CCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FLNGRLDLSQAEAVVDLIRAKTDRAMAAAVAHLRGSLRQAIGRIRERLMEMMAHLEADID
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FPELELEVQTREEVAAGCAWCLGEVERLLGGARTGRILREGLRAVLAGRPNVGKSSLLNR
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LVRENRAIVTPIPGTTRDVIAEWVELGGVPVQLFDTAGLRPTDDPVERIGVARTHEALAQ
HHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCHHHHHHHHHH
AHLVLVVVDAAAGLGPEDREWISQLPQGAARVGVANKIDLNPAFELSALREALGGAPVVG
HHHHEEEEEHHCCCCCCHHHHHHHCCCHHHHCCCCCEECCCCHHHHHHHHHHHCCCCEEE
VSAETGEGFDALEAEVARVAGAFDASEELLVNARQAEAIRRARNHLRDAQATLESGLGDE
ECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LVAIDLRAAWMALGEVTGETAGEELLDQIFSRFCIGK
EEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA