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The map label for this gene is gidA1

Identifier: 51894469

GI number: 51894469

Start: 3560233

End: 3562125

Strand: Reverse

Name: gidA1

Synonym: STH3335

Alternate gene names: 51894469

Gene position: 3562125-3560233 (Counterclockwise)

Preceding gene: 161899035

Following gene: 51894468

Centisome position: 99.89

GC content: 69.62

Gene sequence:

>1893_bases
ATGGGCCTGACCAAGGAGTACGACGTGATCGTGGTCGGCGCCGGGCACGCCGGAATCGAGGCGGCGCTCGCGGCCGCACG
CAAGGGCATGCGCACGGCCTGCTTTACCACGACCCTGGAGAACATCGGAGCGATGAACTGCAACCCGTCCATCGGCGGGC
CCGCGAAGGGGCACCTGGTGCGGGAGATTGACGCCCTGGGCGGGCAGATGGCGCTCACCGCCGATGCCACCTTCCTGCAG
ATGCGGCTCCTGAACAGCGGGAAGGGGCCGGCGGTGCAGGCCCTGCGCGCGCAGATCGACAAGCGGGCGTACGCCTGGGC
GATGCGGCTGGTGCTGGAGCGCACGCCCAACCTGGACCTCAAGCAGGCGATGGTGCAGGAGATCGTGGTGGAGGATGGGC
GGGTGCGCGGGATCGTCACCGCCACCGGCATCTTCTACGGCGCCAAGGCGGTGGTGCTCAGCACCGGCACCTACCTGCAC
GGCCGGACGATCATCGGCGAGGTGCAGCGCTCCTCCGGACCCGGCGGGCTGGCGCCCGCGGTGGGGCTCACCGAGAGCCT
GAAGAGGCTCGGCTTCGAGGTCGGGCGATTCAAGACGGGCACGCCCCCGCGGGTGGACGGGCGCACCGTGGACTTCAGCC
GGATGGTGCGGCAGGACGGCGACCCGGAACCGCACCGGTTCAGCTTCATGTCGCCCCTGGACCACCGGGAGCAGCTGCCC
TGCTGGCTCACCCACACGACCCCGGCGAGCCACGAGCTGATCCGGCGCAACCTGCACCGCGCCCCGATGTTCACGGGCGT
CATCGAGGGGCGTGGGCCCCGGTACTGCCCGTCGGTGGAGGACAAGGTGGTGCGCTTCGCCGATAAGGAGAGCCACCAGG
TCTTCCTGGAGCCCGAGTCGCGGGAGTCCCACGAGATGTACGTGCTGGGCCTCTCCACCAGCCTTCCCGAGGAAGTGCAG
ATCGAACTGGTCCGCACCGTTCCGGGGATGGAGGAGGCCGAGCTCATGCGGCCGGGGTACGCCATCGAGTACGACTACAT
CGTCAGCACGCAGCTGAAGCCCAGCCTGGAGACCAAGCTGGTTCGGGGCCTCTTCTGCGGCGGGCAGATCAACGGTACGT
CGGGTTACGAGGAGGCGGCCGCCCAGGGGTTAATCGCCGGGATCAACGCCGCCTGCTACGTGGAGGGACGGGAGCCCCTG
GTGATCAGCCGCTCGGAGGGGTACATCGGCGTGCTGATCGACGACCTGGTGACCAAAGGCTCGCCGGAGCCGTACCGGAT
GCTGACCTCCCGGGCCGAGTTCCGGATGATGCTCCGGCAGGACAATGCCCACCTCCGGCTCACCGAGAAAGGGCGGGAAT
ACGGCCTTGTGGACGACGCCCGCTGGGAGGTGTTTGTCGCGCTCCGGGACGGCATCGAGGCGGAGCGGGAGCGGCTGGCC
CGGACCGTGGTGGGTCCGGCGCCGGAGGTGCAGCGGGTGCTGGAGCGGCTGGGCTCGGCGCCCCTGAAGGCCGGCGTGAC
CCTGGAGCAGCTGCTGCGGAGGCCCGAGGTGCGCTACGAGCACCTTGTGGAGATGGGTCTGGGCCGTCCGGATCTGGATG
AGGCCATCTGGCGGGAAGTGGAGACGCAGGTCAAGTACGCGGGGTACATCGCGAAGGAGCAACAGCAGGTGGATCGGATG
CGCAGGATGGAGGCCCGGCGGATCCCGCCGACGCTGGATTACCAGGCGCTGACGGGGCTCTCCATGGAAGCCCGGGAGAA
GCTCTCGCGGATCCGGCCGGAGACCCTGGGCCAGGCCAGTCGGATCTCGGGCGTCTCGCCGGCCGACGTGGCGGTCCTGA
TGGTTCACCTGGACCGGCTCGCAAGGGCGCGGGACCGTGCGGAGGAGGCGTAG

Upstream 100 bases:

>100_bases
GTGCCGCCTGGATGGCCCTGGGGGAGGTCACCGGCGAGACGGCCGGCGAGGAGCTGCTGGACCAGATCTTCAGCCGGTTC
TGCATTGGGAAGTGAGCGGG

Downstream 100 bases:

>100_bases
ACAGGAGATGGATGCGACGGCGTACCGGGGCTTGCTGGAAGAGGGATTGGCCAGCCTGGGGGTAGAGGCTGAACCGGGCG
GGGTGGAGGCGGTGCTGCTC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MGLTKEYDVIVVGAGHAGIEAALAAARKGMRTACFTTTLENIGAMNCNPSIGGPAKGHLVREIDALGGQMALTADATFLQ
MRLLNSGKGPAVQALRAQIDKRAYAWAMRLVLERTPNLDLKQAMVQEIVVEDGRVRGIVTATGIFYGAKAVVLSTGTYLH
GRTIIGEVQRSSGPGGLAPAVGLTESLKRLGFEVGRFKTGTPPRVDGRTVDFSRMVRQDGDPEPHRFSFMSPLDHREQLP
CWLTHTTPASHELIRRNLHRAPMFTGVIEGRGPRYCPSVEDKVVRFADKESHQVFLEPESRESHEMYVLGLSTSLPEEVQ
IELVRTVPGMEEAELMRPGYAIEYDYIVSTQLKPSLETKLVRGLFCGGQINGTSGYEEAAAQGLIAGINAACYVEGREPL
VISRSEGYIGVLIDDLVTKGSPEPYRMLTSRAEFRMMLRQDNAHLRLTEKGREYGLVDDARWEVFVALRDGIEAERERLA
RTVVGPAPEVQRVLERLGSAPLKAGVTLEQLLRRPEVRYEHLVEMGLGRPDLDEAIWREVETQVKYAGYIAKEQQQVDRM
RRMEARRIPPTLDYQALTGLSMEAREKLSRIRPETLGQASRISGVSPADVAVLMVHLDRLARARDRAEEA

Sequences:

>Translated_630_residues
MGLTKEYDVIVVGAGHAGIEAALAAARKGMRTACFTTTLENIGAMNCNPSIGGPAKGHLVREIDALGGQMALTADATFLQ
MRLLNSGKGPAVQALRAQIDKRAYAWAMRLVLERTPNLDLKQAMVQEIVVEDGRVRGIVTATGIFYGAKAVVLSTGTYLH
GRTIIGEVQRSSGPGGLAPAVGLTESLKRLGFEVGRFKTGTPPRVDGRTVDFSRMVRQDGDPEPHRFSFMSPLDHREQLP
CWLTHTTPASHELIRRNLHRAPMFTGVIEGRGPRYCPSVEDKVVRFADKESHQVFLEPESRESHEMYVLGLSTSLPEEVQ
IELVRTVPGMEEAELMRPGYAIEYDYIVSTQLKPSLETKLVRGLFCGGQINGTSGYEEAAAQGLIAGINAACYVEGREPL
VISRSEGYIGVLIDDLVTKGSPEPYRMLTSRAEFRMMLRQDNAHLRLTEKGREYGLVDDARWEVFVALRDGIEAERERLA
RTVVGPAPEVQRVLERLGSAPLKAGVTLEQLLRRPEVRYEHLVEMGLGRPDLDEAIWREVETQVKYAGYIAKEQQQVDRM
RRMEARRIPPTLDYQALTGLSMEAREKLSRIRPETLGQASRISGVSPADVAVLMVHLDRLARARDRAEEA
>Mature_629_residues
GLTKEYDVIVVGAGHAGIEAALAAARKGMRTACFTTTLENIGAMNCNPSIGGPAKGHLVREIDALGGQMALTADATFLQM
RLLNSGKGPAVQALRAQIDKRAYAWAMRLVLERTPNLDLKQAMVQEIVVEDGRVRGIVTATGIFYGAKAVVLSTGTYLHG
RTIIGEVQRSSGPGGLAPAVGLTESLKRLGFEVGRFKTGTPPRVDGRTVDFSRMVRQDGDPEPHRFSFMSPLDHREQLPC
WLTHTTPASHELIRRNLHRAPMFTGVIEGRGPRYCPSVEDKVVRFADKESHQVFLEPESRESHEMYVLGLSTSLPEEVQI
ELVRTVPGMEEAELMRPGYAIEYDYIVSTQLKPSLETKLVRGLFCGGQINGTSGYEEAAAQGLIAGINAACYVEGREPLV
ISRSEGYIGVLIDDLVTKGSPEPYRMLTSRAEFRMMLRQDNAHLRLTEKGREYGLVDDARWEVFVALRDGIEAERERLAR
TVVGPAPEVQRVLERLGSAPLKAGVTLEQLLRRPEVRYEHLVEMGLGRPDLDEAIWREVETQVKYAGYIAKEQQQVDRMR
RMEARRIPPTLDYQALTGLSMEAREKLSRIRPETLGQASRISGVSPADVAVLMVHLDRLARARDRAEEA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=652, Percent_Identity=42.3312883435583, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI19882217, Length=677, Percent_Identity=40.768094534712, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI183227703, Length=692, Percent_Identity=39.8843930635838, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=52.520325203252, Blast_Score=635, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=630, Percent_Identity=39.8412698412698, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6321202, Length=638, Percent_Identity=41.2225705329154, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24658174, Length=644, Percent_Identity=44.2546583850932, Blast_Score=470, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_SYMTH (Q67J34)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_077160.1
- ProteinModelPortal:   Q67J34
- SMR:   Q67J34
- GeneID:   2980038
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3335
- NMPDR:   fig|292459.1.peg.3201
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   STHE292459:STH3335-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR013027
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69759; Mature: 69628

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTKEYDVIVVGAGHAGIEAALAAARKGMRTACFTTTLENIGAMNCNPSIGGPAKGHLV
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
REIDALGGQMALTADATFLQMRLLNSGKGPAVQALRAQIDKRAYAWAMRLVLERTPNLDL
HHHHHCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
KQAMVQEIVVEDGRVRGIVTATGIFYGAKAVVLSTGTYLHGRTIIGEVQRSSGPGGLAPA
HHHHHHHHHHCCCCEEEEEEECHHHHCCEEEEEECCCEEECCHHHHHHHCCCCCCCCCHH
VGLTESLKRLGFEVGRFKTGTPPRVDGRTVDFSRMVRQDGDPEPHRFSFMSPLDHREQLP
HHHHHHHHHHCHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCEECCCCCCHHHCCC
CWLTHTTPASHELIRRNLHRAPMFTGVIEGRGPRYCPSVEDKVVRFADKESHQVFLEPES
EEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCC
RESHEMYVLGLSTSLPEEVQIELVRTVPGMEEAELMRPGYAIEYDYIVSTQLKPSLETKL
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEECCCCCHHHHHH
VRGLFCGGQINGTSGYEEAAAQGLIAGINAACYVEGREPLVISRSEGYIGVLIDDLVTKG
HHHHEECCEECCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCEEEEEEHHHHHCC
SPEPYRMLTSRAEFRMMLRQDNAHLRLTEKGREYGLVDDARWEVFVALRDGIEAERERLA
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
RTVVGPAPEVQRVLERLGSAPLKAGVTLEQLLRRPEVRYEHLVEMGLGRPDLDEAIWREV
HHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHH
ETQVKYAGYIAKEQQQVDRMRRMEARRIPPTLDYQALTGLSMEAREKLSRIRPETLGQAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCHHCCCHH
RISGVSPADVAVLMVHLDRLARARDRAEEA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLTKEYDVIVVGAGHAGIEAALAAARKGMRTACFTTTLENIGAMNCNPSIGGPAKGHLV
CCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
REIDALGGQMALTADATFLQMRLLNSGKGPAVQALRAQIDKRAYAWAMRLVLERTPNLDL
HHHHHCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
KQAMVQEIVVEDGRVRGIVTATGIFYGAKAVVLSTGTYLHGRTIIGEVQRSSGPGGLAPA
HHHHHHHHHHCCCCEEEEEEECHHHHCCEEEEEECCCEEECCHHHHHHHCCCCCCCCCHH
VGLTESLKRLGFEVGRFKTGTPPRVDGRTVDFSRMVRQDGDPEPHRFSFMSPLDHREQLP
HHHHHHHHHHCHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCEECCCCCCHHHCCC
CWLTHTTPASHELIRRNLHRAPMFTGVIEGRGPRYCPSVEDKVVRFADKESHQVFLEPES
EEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCC
RESHEMYVLGLSTSLPEEVQIELVRTVPGMEEAELMRPGYAIEYDYIVSTQLKPSLETKL
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEECCCCCHHHHHH
VRGLFCGGQINGTSGYEEAAAQGLIAGINAACYVEGREPLVISRSEGYIGVLIDDLVTKG
HHHHEECCEECCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCEEEEEEHHHHHCC
SPEPYRMLTSRAEFRMMLRQDNAHLRLTEKGREYGLVDDARWEVFVALRDGIEAERERLA
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
RTVVGPAPEVQRVLERLGSAPLKAGVTLEQLLRRPEVRYEHLVEMGLGRPDLDEAIWREV
HHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHH
ETQVKYAGYIAKEQQQVDRMRRMEARRIPPTLDYQALTGLSMEAREKLSRIRPETLGQAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCHHCCCHH
RISGVSPADVAVLMVHLDRLARARDRAEEA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA