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The map label for this gene is trmA

Identifier: 161870527

GI number: 161870527

Start: 1621157

End: 1622107

Strand: Reverse

Name: trmA

Synonym: NMCC_1592

Alternate gene names: 161870527

Gene position: 1622107-1621157 (Counterclockwise)

Preceding gene: 161870530

Following gene: 161870526

Centisome position: 75.33

GC content: 48.9

Gene sequence:

>951_bases
ATGTTTTATGCCATGTTTGAAAAAGGACAAAAGGCTGGCGGGGCAAGCCTGATCCGTTGCGACCGTTTTGAAGCGGCTTC
CGAAGCCGTCAACCGCCTGATGCCAGAATTGATTGCCGTTGCCGCCCAATCTGCCGAACTGAGAAACCGTTGGTATGCCG
TCGAATTTTTGTCTACGCTCAGCGGGGAAATGCTGGTTACCATGATTTACCACAAAAGGCTTGATAATGAGTGGATGCAG
GCGGCGCAAGCGTTACAGCAACAGTTGGATATTTCCGTCATCGGGCGGAGCAGGGGACAGAAAATAGTCTTAAAACAGGA
CTATGTAACGGAAACTTTGAAGGTCGGCAACCGGGATTTCCGTTATCGGCAAATTGAAGGCAGTTTTACCCAACCGAATG
CCGCCGTGTGTCAGAAAATGCTTGAATGGGCCTGCGGTGCAGCAGAAGGATTGGATGGGGATTTGCTCGAACTGTATTGC
GGAAACGGCAACTTTACGCTTCCTTTATCTGAAAAGTTTGAACGTGTTTTGGCAACCGAAATCTCCAAAACCTCCGTCAG
CGCGGCGCAATGGAATATCGAAGCCAACCGCATCGGCAACATCAAAATCGCCCGTCTGTCTGCTGAAGAATTTACCGAAG
CCTATACCGGAAAACGTGAGTTTAAACGCCTTAAGGATGGCGGTATTGCTTTGACGGATTACGCATTTTCCACTATTTTC
GTCGATCCGCCGCGTGCCGGTATCGATGAGGAAACTTTGAAGCTGGTGTCGCAGTTTGACAACATCATCTACATTTCCTG
CAATCCCGAAACGCTGCGTACCAATTTGGACACCTTGGCGGAAACCCATACGGTTGAACGTGCCGCCCTGTTCGATCAAT
TTCCGTTTACGCACCATATTGAAAGCGGTGTGCTGTTGAAAAAGAAAATCCTTGGAAAAAGCAAGAGGTAA

Upstream 100 bases:

>100_bases
TTTTGCAGGTTTGGATGTTCCTGAGTGGGAAGTGTACGAATCTCCGGACAAACATTACCGTATGCGTGCCGAGTTCCGTA
TTTGGCACGAAGGCGGTGAA

Downstream 100 bases:

>100_bases
GGACTATGTACCGACACATCGAATACTATCCCGGCGATCCGATTTTGAGTTTGGTCGAAACCTTCAAAAACGACCCGCGC
CCCGAAAAAGTCAATTTGAG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRWYAVEFLSTLSGEMLVTMIYHKRLDNEWMQ
AAQALQQQLDISVIGRSRGQKIVLKQDYVTETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYC
GNGNFTLPLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLKDGGIALTDYAFSTIF
VDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETHTVERAALFDQFPFTHHIESGVLLKKKILGKSKR

Sequences:

>Translated_316_residues
MFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRWYAVEFLSTLSGEMLVTMIYHKRLDNEWMQ
AAQALQQQLDISVIGRSRGQKIVLKQDYVTETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYC
GNGNFTLPLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLKDGGIALTDYAFSTIF
VDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETHTVERAALFDQFPFTHHIESGVLLKKKILGKSKR
>Mature_316_residues
MFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRWYAVEFLSTLSGEMLVTMIYHKRLDNEWMQ
AAQALQQQLDISVIGRSRGQKIVLKQDYVTETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYC
GNGNFTLPLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLKDGGIALTDYAFSTIF
VDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETHTVERAALFDQFPFTHHIESGVLLKKKILGKSKR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Escherichia coli, GI1790403, Length=314, Percent_Identity=53.5031847133758, Blast_Score=346, Evalue=9e-97,
Organism=Escherichia coli, GI1789148, Length=188, Percent_Identity=33.5106382978723, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24666579, Length=170, Percent_Identity=30, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_NEIM0 (A9M1Q1)

Other databases:

- EMBL:   CP000381
- RefSeq:   YP_001599699.1
- ProteinModelPortal:   A9M1Q1
- SMR:   A9M1Q1
- EnsemblBacteria:   EBNEIT00000012496
- GeneID:   5796943
- GenomeReviews:   CP000381_GR
- KEGG:   nmn:NMCC_1592
- GeneTree:   EBGT00050000020231
- HOGENOM:   HBG608402
- ProtClustDB:   PRK05031
- BioCyc:   NMEN374833:NMCC_1592-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 35586; Mature: 35586

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 318-318 BINDING 182-182 BINDING 210-210 BINDING 215-215 BINDING 231-231 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRWYAVEFLSTL
CEEHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYVTETLKVGNRDF
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHHCCCCCC
RYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTLPLSEKFERVLATE
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECHHHHHHHHHHHH
ISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLKDGGIALTDYAFSTIF
HHHHCCHHHEEEEECCCCCCEEEEEECHHHHHHHHCCHHHHHHHHCCCEEEEEECEEEEE
VDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETHTVERAALFDQFPFTHHI
ECCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
ESGVLLKKKILGKSKR
CCCHHHHHHHHCCCCC
>Mature Secondary Structure
MFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRWYAVEFLSTL
CEEHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYVTETLKVGNRDF
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHHCCCCCC
RYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTLPLSEKFERVLATE
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECHHHHHHHHHHHH
ISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLKDGGIALTDYAFSTIF
HHHHCCHHHEEEEECCCCCCEEEEEECHHHHHHHHCCHHHHHHHHCCCEEEEEECEEEEE
VDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETHTVERAALFDQFPFTHHI
ECCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
ESGVLLKKKILGKSKR
CCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA