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The map label for this gene is metE [H]

Identifier: 161869856

GI number: 161869856

Start: 887635

End: 889911

Strand: Direct

Name: metE [H]

Synonym: NMCC_0887

Alternate gene names: 161869856

Gene position: 887635-889911 (Clockwise)

Preceding gene: 161869855

Following gene: 161869857

Centisome position: 41.22

GC content: 52.7

Gene sequence:

>2277_bases
ATGACAACATTACATTTTTCAGGCTTCCCGCGTGTCGGTGCCTTCCGCGAATTGAAATTTGCCCAAGAAAAATACTGGCG
CAAAGAAATCAGCGAGCAAGAATTGCTGGCTGTTGCCAAAGACTTGCGCGAGAAAAACTGGAAACACCAAGCCGCTGCCA
ACGCCGATTACGTTGCCGTAGGCGATTTCACTTTCTACGATCACATCCTCGACCTGCAAGTCGCTACCGGCGCGATTCCC
GCCCGCTTCGGCTTCGACAGCCAAAACCTGTCTTTGGAACAATTCTTCCAACTGGCGCGCGGCAACAAAGACCAATTCGC
TATCGAAATGACCAAATGGTTCGACACCAACTATCACTACTTGGTGCCTGAATTCCACGCCGATACCGAATTCAAAGCCA
ACGCCAAACACTACGTTCAACAACTGCAAGAAGCCCAAGCTTTGGGCTTGAAAGCCAAACCGACCGTTGTTGGTCCGCTG
ACTTTCCTGTGGGTAGGTAAAGAAAAAGGTGCGGTTGAATTCGACCGTCTGAGCCTGTTGCCTAAACTGTTGCCTGTTTA
CGTTGAAATCCTGACTGCGTTGGTTGAAGCCGGTGCCGAGTGGATTCAAATCGACGAGCCTGCTTTGACTGTCGATCTGC
CTAAAGAATGGGTAGAAGCATACAAAGACGTTTACGCTACTTTGAGCAAAGTAAGTGCCAAAATCCTGTTGGGCACTTAC
TTCGGTTCTGTTGCCGAACACGCCGCATTGCTGAAAGCCCTGCCCGTTGACGGCCTGCACATCGACTTGGTACGCGCTCC
CGAGCAACCGGACGCGTTCGCCGACTACGACAAAGTCCTGTCTGTCGGCGTGATTGACGGCCGCAACATTTGGCGCGCCA
ACCTGAACAAAGTTTTGGAAACTGTTGAGCCTCTGCAAGCCAAACTGGGCGAGCGTTTGTGGATTTCCAGCTCTTGCTCG
CTGTTGCACACTCCGTTTGACTTGTCAGTTGAAGAAAAACTGAAAGCCAACAAACCCGACCTGTACTCTTGGTTGGCATT
TACCCTGCAAAAAACCCAAGAATTGCGCGTTCTGAAAGCCGCATTGAACGAAGGTCGTGATTCTGTTGCCGAAGAACTCG
CCGCCAGCCAAGCTGCCGCTGATTCACGCGCCAACAGCAGCGAAATCCACCGCGCGGACGTTGCCAAACGCCTGGCCGAT
TTGCCTGCCAACGCAGACCAACGCAAATCACCGTTTGTCAACCGTATCAAAGCGCAACAAGCATGGTTGAACCTGCCTCT
GCTGCCGACGACCAACATCGGTTCTTTCCCGCAAACTACCGAAATTCGTCAAGCACGCGCCGCCTTCAAAAAAGGCGAAC
TGTCTGCCGCCGATTACGAAGCCGCGATGAAAAAAGAAATCGCCTTGGTGGTTGAAGAGCAAGAAAAACTGGACTTGGAC
GTACTGGTACACGGCGAAGCCGAGCGTAACGACATGGTCGAATACTTCGGCGAACTGTTGAGCGGTTTTGCATTCACCCA
ATACGGCTGGGTACAAAGCTACGGCTCACGCTGCGTGAAACCACCGATTATCTTCGGTGACGTAAGCCGTCCTGAAGCCA
TGACTGTCGCTTGGTCTACTTACGCTCAAAGCCTGACCAAACGCCCGATGAAAGGTATGTTGACCGGCCCTGTAACCATT
CTGCAATGGTCTTTCGTCCGCAACGATATTCCCCGCGCTACCGTGTGCAAACAAATCGCACTGGCTCTGAACGACGAAGT
ATTGGATCTGGAAAAAGCAGGCATCAAAGTCATCCAAATCGACGAACCTGCCATCCGCGAAGGCCTGCCTTTGAAACGTG
CCGATTGGGATGCCTACCTGAACTGGGCCGGCGAATCTTTCCGCCTGTCCTCTGCCGGTTGTGAAGACAGCACTCAGATC
CACACCCATATGTGCTACTCCGAGTTCAACGACATCCTGCCGGCCATCGCAGCTATGGATGCCGACGTGATTACTATCGA
AACTTCACGTTCCGATATGGAACTCCTGACCGCGTTCGGCAAGTTCAAATACCCGAACGACATCGGCCCGGGCGTTTACG
ACATCCACAGCCCGCGCGTACCGACTGAAGCCGAAGTGGAACACCTGTTGCGTAAAGCCATCGAAGTCGTACCGGTTGAG
CGTCTGTGGGTGAATCCTGACTGCGGCCTGAAAACACGCGGCTGGAAAGAAACTCTGGAACAACTCCAAGTGATGATGAA
CGTAACCCGCAAACTGCGTGCCGAACTGGCGAAATAA

Upstream 100 bases:

>100_bases
TCTGAAAAGCAAAACGCCGCCCCATCTAAGCCATTCTGATTTACAATACCGGCCGATTCGGATCGAACCGGTCCTTACAA
AATCCAACTGGAGAGTTTAT

Downstream 100 bases:

>100_bases
GCCGGAACCGTATGAACAAATGCCGTCTGAAAGCCCTTCAGACGGCATTTTGCCCTGAGTTGCGGCGCAAGGGTGCAGTT
GCCGGGAAATTTTCTCATTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLREKNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLSLEQFFQLARGNKDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWVEAYKDVYATLSKVSAKILLGTY
FGSVAEHAALLKALPVDGLHIDLVRAPEQPDAFADYDKVLSVGVIDGRNIWRANLNKVLETVEPLQAKLGERLWISSSCS
LLHTPFDLSVEEKLKANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRADVAKRLAD
LPANADQRKSPFVNRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFKKGELSAADYEAAMKKEIALVVEEQEKLDLD
VLVHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTI
LQWSFVRNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLSSAGCEDSTQI
HTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGKFKYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVE
RLWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK

Sequences:

>Translated_758_residues
MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLREKNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLSLEQFFQLARGNKDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWVEAYKDVYATLSKVSAKILLGTY
FGSVAEHAALLKALPVDGLHIDLVRAPEQPDAFADYDKVLSVGVIDGRNIWRANLNKVLETVEPLQAKLGERLWISSSCS
LLHTPFDLSVEEKLKANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRADVAKRLAD
LPANADQRKSPFVNRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFKKGELSAADYEAAMKKEIALVVEEQEKLDLD
VLVHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTI
LQWSFVRNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLSSAGCEDSTQI
HTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGKFKYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVE
RLWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK
>Mature_757_residues
TTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLREKNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPA
RFGFDSQNLSLEQFFQLARGNKDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPLT
FLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWVEAYKDVYATLSKVSAKILLGTYF
GSVAEHAALLKALPVDGLHIDLVRAPEQPDAFADYDKVLSVGVIDGRNIWRANLNKVLETVEPLQAKLGERLWISSSCSL
LHTPFDLSVEEKLKANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRADVAKRLADL
PANADQRKSPFVNRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFKKGELSAADYEAAMKKEIALVVEEQEKLDLDV
LVHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTIL
QWSFVRNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAGESFRLSSAGCEDSTQIH
THMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGKFKYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVER
LWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=761, Percent_Identity=54.796320630749, Blast_Score=821, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=768, Percent_Identity=47.5260416666667, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85086; Mature: 84955

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLREKNWKHQAAANADYVAV
CCEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGNKDQFAIEMTKWFDTNYHY
CCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHCCCCCEE
LVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPLTFLWVGKEKGAVEFDRLSLL
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCCCHHHHHHH
PKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWVEAYKDVYATLSKVSAKILLGTY
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSVAEHAALLKALPVDGLHIDLVRAPEQPDAFADYDKVLSVGVIDGRNIWRANLNKVLE
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHH
TVEPLQAKLGERLWISSSCSLLHTPFDLSVEEKLKANKPDLYSWLAFTLQKTQELRVLKA
HHHHHHHHHCCEEEECCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ALNEGRDSVAEELAASQAAADSRANSSEIHRADVAKRLADLPANADQRKSPFVNRIKAQQ
HHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AWLNLPLLPTTNIGSFPQTTEIRQARAAFKKGELSAADYEAAMKKEIALVVEEQEKLDLD
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCEE
VLVHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWST
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEHHHH
YAQSLTKRPMKGMLTGPVTILQWSFVRNDIPRATVCKQIALALNDEVLDLEKAGIKVIQI
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEEEE
DEPAIREGLPLKRADWDAYLNWAGESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMD
CCHHHHCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ADVITIETSRSDMELLTAFGKFKYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVE
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHH
RLWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK
HEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLREKNWKHQAAANADYVAV
CEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGNKDQFAIEMTKWFDTNYHY
CCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHCCCCCEE
LVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPLTFLWVGKEKGAVEFDRLSLL
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCCCHHHHHHH
PKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWVEAYKDVYATLSKVSAKILLGTY
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSVAEHAALLKALPVDGLHIDLVRAPEQPDAFADYDKVLSVGVIDGRNIWRANLNKVLE
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHH
TVEPLQAKLGERLWISSSCSLLHTPFDLSVEEKLKANKPDLYSWLAFTLQKTQELRVLKA
HHHHHHHHHCCEEEECCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ALNEGRDSVAEELAASQAAADSRANSSEIHRADVAKRLADLPANADQRKSPFVNRIKAQQ
HHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AWLNLPLLPTTNIGSFPQTTEIRQARAAFKKGELSAADYEAAMKKEIALVVEEQEKLDLD
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCEE
VLVHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWST
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEHHHH
YAQSLTKRPMKGMLTGPVTILQWSFVRNDIPRATVCKQIALALNDEVLDLEKAGIKVIQI
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEEEE
DEPAIREGLPLKRADWDAYLNWAGESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMD
CCHHHHCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ADVITIETSRSDMELLTAFGKFKYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVE
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHH
RLWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK
HEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA