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The map label for this gene is metF [H]

Identifier: 161869855

GI number: 161869855

Start: 886724

End: 887500

Strand: Direct

Name: metF [H]

Synonym: NMCC_0886

Alternate gene names: 161869855

Gene position: 886724-887500 (Clockwise)

Preceding gene: 161869849

Following gene: 161869856

Centisome position: 41.18

GC content: 55.34

Gene sequence:

>777_bases
ATGGAAACGATGCTGTGGGATTCCATCCATCGTCTGCAAACCCTGCATCCCAAGTTCGTATCCGTAACTTACGGTGCCAA
CTCTGGCGAACGCGACCGCACGCACGGCATCGTCAAACGCATCAAGCAGGAAACCGGCTTGGAGGCCGCGCCTCACCTGA
CCGGTATCGACGCTTCTCCCGACGAATTGCGCCAAATTGCCAAAGATTATTGGGACAGCGGTATCCGCCGCATTGTCGCC
CTGCGCGGCGACGAGCCGCCCGGTTACGAGAAAAAACCGTTTTACGCCGAAGACTTGGTCAAACTGTTGCGCTCCGTCGC
CGACTTCGACATTTCTGTAGCGGCATATCCCGAAGTGCATCCCGAAGCGAAATCCGCACAAGCCGACCTGATTAATTTGA
AACGCAAAATCGATGCGGGCGCGAACCACGTCATCACCCAATTCTTCTTCGATGTGGAACGCTACCTGCGCTTCCGCGAC
CGCTGCGTGATGTTGGGCATCGATGTGGAAATCGTCCCCGGCATCCTGCCCGTTACCAACTTCAAGCAGCTCGGCAAAAT
GGCGCAGGTAACCAACGTCAAAATCCCAAGCTGGCTGTCGCAAATGTATGAAGGTTTGGACGACGACCAAGGCACGCGCA
ATCTGGTGGCGGCAAGTATCGCCATCGATATGGTCAAAGTCCTGTCCCGCGAAGGCGTGAAAGATTTCCACTTCTATACG
CTTAACCGCAGCGAGCTGACTTACGCCATCTGCCATATTTTAGGCGTGCGCCCTTAA

Upstream 100 bases:

>100_bases
GAATTACGCAAAAGAAATCAATGCGTTAAACAACAGCCTTTCCGATTTGAAAGGCGATATCAACGTTTCGTTTGAATTTT
TCCCGCCGAAAAACGAACAA

Downstream 100 bases:

>100_bases
AGCTGAAAACGGCTTCAGACGGCATTTGAGGCCGTCTGAAAAGCAAAACGCCGCCCCATCTAAGCCATTCTGATTTACAA
TACCGGCCGATTCGGATCGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
METMLWDSIHRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAKDYWDSGIRRIVA
LRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVITQFFFDVERYLRFRD
RCVMLGIDVEIVPGILPVTNFKQLGKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYT
LNRSELTYAICHILGVRP

Sequences:

>Translated_258_residues
METMLWDSIHRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAKDYWDSGIRRIVA
LRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVITQFFFDVERYLRFRD
RCVMLGIDVEIVPGILPVTNFKQLGKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYT
LNRSELTYAICHILGVRP
>Mature_258_residues
METMLWDSIHRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAKDYWDSGIRRIVA
LRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVITQFFFDVERYLRFRD
RCVMLGIDVEIVPGILPVTNFKQLGKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYT
LNRSELTYAICHILGVRP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=259, Percent_Identity=35.1351351351351, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790377, Length=258, Percent_Identity=76.7441860465116, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25147436, Length=261, Percent_Identity=30.6513409961686, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=261, Percent_Identity=30.6513409961686, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=278, Percent_Identity=32.0143884892086, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=267, Percent_Identity=30.3370786516854, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24662658, Length=223, Percent_Identity=26.457399103139, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 29212; Mature: 29212

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMLWDSIHRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASP
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
DELRQIAKDYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCC
PEAKSAQADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEECCCCCCCCC
FKQLGKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYT
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LNRSELTYAICHILGVRP
ECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METMLWDSIHRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASP
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
DELRQIAKDYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCC
PEAKSAQADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEECCCCCCCCC
FKQLGKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYT
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LNRSELTYAICHILGVRP
ECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]