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The map label for this gene is pnp [H]

Identifier: 161869702

GI number: 161869702

Start: 726149

End: 728269

Strand: Direct

Name: pnp [H]

Synonym: NMCC_0722

Alternate gene names: 161869702

Gene position: 726149-728269 (Clockwise)

Preceding gene: 161869701

Following gene: 161869705

Centisome position: 33.72

GC content: 53.65

Gene sequence:

>2121_bases
ATGTTCGACAAACACGTTAAAACTTTCCAATACGGCAACCACACCGTTACTTTGGAAACCGGCGAAATTGCCCGCCAAGC
CGCCGCTGCCGTTAAAGTCTCTATGGGCGACACCGTTGTTTTGGTTGCCGTTACCACCAACAAAGAAGTGAAAGAAGGTC
AAGACTTCTTCCCCCTGACCGTCGATTATTTGGAACGCACTTACGCCGCAGGTAAAATCCCCGGCGGTTTCTTCAAACGC
GAAGGCAAACAAAGCGAAAAAGAAATCCTGACCAGCCGTCTGATCGACCGCCCGATTCGTCCGCTGTTCCCCGAAGGTTT
CTACCACGACATCCAAATCGTAGCGATGGTCGTGTCCGTCGATCCCGAAATCGATTCCGACATTCCTGCAATGTTGGGCG
CGTCTGCCGCGCTGGTGTTGAGCGGCGTACCGTTTGCCGGTCCGATTGGTGCGGCACGCGTGGGTTATATAAACGGCGTG
TACGTTTTGAACCCGACTAAAGCCGAATTGGCTAAATCGCAATTGGACTTGGTGGTCGCCGGTACGTCCAAAGCCGTGTT
GATGGTGGAATCCGAAGCCAAAATCCTGCCCGAAGACGTGATGTTGGGCGCGGTGGTTTACGGCCACGATCAAATGCAGG
CGGCGATTAACGCCATCAATGAATTTGCCGACGAAGTCAATCCGGAAGTTTGGGATTGGAAAGCACCTGAAACCAATGAA
GAATTAGTTGCCAAAGTCCGCGGGATTGCCGGCGAAACCATTAAAGAAGCGTTCAAAATCCGTCAAAAACAAGCGCGTTC
CGCCAAATTGGACGAGGCTTGGAATGCGGTAAAAGAAGCCTTGATTACCGAAGAAACCGATACTTTGGCCGCCAACGAAA
TCAAAGGTATTTTCAAACACTTGGAAGCCGATGTTGTCCGCAGCCAAATTTTGGACGGTCAACCGCGCATCGACGGCCGC
GACACCCGCACCGTCCGTCCTTTGAACATCCAAACCGGCGTATTGCCGCGTACACACGGTTCCGCATTGTTTACCCGTGG
CGAAACCCAAGCTTTGGCGGTTGCAACTTTGGGTACTTCGCGCGACGAGCAAATCATTGACGCGCTGTCCGGCGAATACA
CCGACCGCTTCATGCTGCACTACAACTTTCCGCCGTACTCTACCGGAGAAGTGGGTCGCGTAGGTGCGCCGAAACGCCGT
GAAATCGGTCACGGCCGTTTGGCTAAACGTGCATTGTTGGCCGTATTGCCGAAACCTGAAGATTTCAGCTACACCATGCG
CGTGGTCTCCGAAATTACCGAATCCAACGGCTCTTCCTCTATGGCTTCCGTCTGCGGCGGCTGCCTGAGCCTGCTGTCTG
CCGGCGTGCCTTTGAAAGCACACGTTGCCGGTATCGCGATGGGTCTGATTCTGGAAGGCAACAAATTTGCCGTCCTGACC
GACATTTTGGGCGACGAAGACCACTTGGGCGATATGGACTTTAAAGTGGCCGGTACGACCGAAGGCGTTACCGCGCTGCA
AATGGACATCAAAATCCAAGGCATTACCAAAGAAATTATGCAAATCGCTTTGGCACAGGCCAAAGAAGCGCGTCTGCACA
TCTTGGATCAGATGAAAGCCGCCGTTGCGGGCCCGCAAGAGTTGTCCGCACACGCGCCGCGTTTGTTTACGATGAAAATC
AACCAAGACAAAATCCGCGAAGTTATCGGTAAGGGCGGCGAAACCATCCGTTCGATTACCGCTGAAACCGGTACGGAAAT
CAATATTGCCGAAGACGGTACGATTACCATTGCTGCGACCACTCAAGAAGCCGGCGATGCGGCGAAAAAACGTATCGAGC
AGATTACTGCCGAAGTGGAAGTGGGCAAAGTGTATGAAGGCACTGTGGTGAAAATCCTCGACAACAACGTCGGCGCGATT
GTCAGCGTGATGCCGGGCAAAGACGGTTTGGTACACATCAGCCAAATCGCCCACGAGCGCGTACGCAATGTCGGTGACTA
CCTGCAAGTCGGTCAGGTGGTGAACGTGAAAGCATTGGAAGTGGATGACAGAGGCCGTGTGCGCCTGTCCATCAAAGCCC
TGCTGGACGCGCCTGCCCGTGAGGAAAATGCCGCCGAGTAA

Upstream 100 bases:

>100_bases
TTACAACCTGCCGCACGGTCTGAAACCCTAACTATGCACATTCGGATTTTAGTGTGCATTATTAGTGTTTTAGCAGTGCG
GTATTTTGAAAGGAACAATA

Downstream 100 bases:

>100_bases
CGCTTAGGGTGAAAGTGCCGTCTGAACAGGTTTCAGACGGTATTTTTTACGGGTATCGGGAATGAATGGGGCTTACAGCC
ACAGGACGGCAAGTTTCCAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAVTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKR
EGKQSEKEILTSRLIDRPIRPLFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINAINEFADEVNPEVWDWKAPETNE
ELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNAVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGR
DTRTVRPLNIQTGVLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVGRVGAPKRR
EIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILEGNKFAVLT
DILGDEDHLGDMDFKVAGTTEGVTALQMDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKI
NQDKIREVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKVYEGTVVKILDNNVGAI
VSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVDDRGRVRLSIKALLDAPAREENAAE

Sequences:

>Translated_706_residues
MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAVTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKR
EGKQSEKEILTSRLIDRPIRPLFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINAINEFADEVNPEVWDWKAPETNE
ELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNAVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGR
DTRTVRPLNIQTGVLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVGRVGAPKRR
EIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILEGNKFAVLT
DILGDEDHLGDMDFKVAGTTEGVTALQMDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKI
NQDKIREVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKVYEGTVVKILDNNVGAI
VSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVDDRGRVRLSIKALLDAPAREENAAE
>Mature_706_residues
MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAVTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKR
EGKQSEKEILTSRLIDRPIRPLFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINAINEFADEVNPEVWDWKAPETNE
ELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNAVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGR
DTRTVRPLNIQTGVLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVGRVGAPKRR
EIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILEGNKFAVLT
DILGDEDHLGDMDFKVAGTTEGVTALQMDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKI
NQDKIREVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKVYEGTVVKILDNNVGAI
VSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVDDRGRVRLSIKALLDAPAREENAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=717, Percent_Identity=37.7963737796374, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=63.2034632034632, Blast_Score=878, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=708, Percent_Identity=32.0621468926554, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI281362905, Length=733, Percent_Identity=37.1077762619372, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651641, Length=733, Percent_Identity=37.1077762619372, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651643, Length=733, Percent_Identity=37.1077762619372, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI161079377, Length=674, Percent_Identity=37.240356083086, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76237; Mature: 76237

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAVTTNKEVKEGQDFFPLT
CCCCCCCEEEECCEEEEEECHHHHHHHHHHEEEECCCEEEEEEEECCCHHHCCCCCCCEE
VDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPLFPEGFYHDIQIVAMVVSV
HHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEEEC
DPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGVYVLNPTKAELAKSQLDLVVA
CCCCCCCCHHHHCCCHHHEEECCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHEEEEEE
GTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINAINEFADEVNPEVWDWKAPETNE
CCCCEEEEEECCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
ELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNAVKEALITEETDTLAANEIKGIFKH
HHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGVLPRTHGSALFTRGETQALAVATLGTS
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCC
RDEQIIDALSGEYTDRFMLHYNFPPYSTGEVGRVGAPKRREIGHGRLAKRALLAVLPKPE
CHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCC
DFSYTMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILEGNKFAVLT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEEE
DILGDEDHLGDMDFKVAGTTEGVTALQMDIKIQGITKEIMQIALAQAKEARLHILDQMKA
ECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAGPQELSAHAPRLFTMKINQDKIREVIGKGGETIRSITAETGTEINIAEDGTITIAAT
HHCCHHHHHCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEEE
TQEAGDAAKKRIEQITAEVEVGKVYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHER
CCHHHHHHHHHHHHHHHHEEECEEECCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
VRNVGDYLQVGQVVNVKALEVDDRGRVRLSIKALLDAPAREENAAE
HHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEEHHCCCCCCCCCCC
>Mature Secondary Structure
MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAVTTNKEVKEGQDFFPLT
CCCCCCCEEEECCEEEEEECHHHHHHHHHHEEEECCCEEEEEEEECCCHHHCCCCCCCEE
VDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPLFPEGFYHDIQIVAMVVSV
HHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEEEC
DPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGVYVLNPTKAELAKSQLDLVVA
CCCCCCCCHHHHCCCHHHEEECCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHEEEEEE
GTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINAINEFADEVNPEVWDWKAPETNE
CCCCEEEEEECCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
ELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNAVKEALITEETDTLAANEIKGIFKH
HHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGVLPRTHGSALFTRGETQALAVATLGTS
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCC
RDEQIIDALSGEYTDRFMLHYNFPPYSTGEVGRVGAPKRREIGHGRLAKRALLAVLPKPE
CHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCC
DFSYTMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILEGNKFAVLT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEEE
DILGDEDHLGDMDFKVAGTTEGVTALQMDIKIQGITKEIMQIALAQAKEARLHILDQMKA
ECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAGPQELSAHAPRLFTMKINQDKIREVIGKGGETIRSITAETGTEINIAEDGTITIAAT
HHCCHHHHHCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEEE
TQEAGDAAKKRIEQITAEVEVGKVYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHER
CCHHHHHHHHHHHHHHHHEEECEEECCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
VRNVGDYLQVGQVVNVKALEVDDRGRVRLSIKALLDAPAREENAAE
HHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEEHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA