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The map label for this gene is dapF [H]

Identifier: 161869705

GI number: 161869705

Start: 729457

End: 730308

Strand: Direct

Name: dapF [H]

Synonym: NMCC_0725

Alternate gene names: 161869705

Gene position: 729457-730308 (Clockwise)

Preceding gene: 161869702

Following gene: 161869706

Centisome position: 33.87

GC content: 55.63

Gene sequence:

>852_bases
ATGAACACGTTGAAATTTACCAAAATGCACGGTTTGGGCAACGATTTTATGGTGATTGACGCGGTCAGTCAGGATTTTAC
CCCCGAGGACGCGCCGATTGCGGCATGGGCGGACCGCTTCCGAGGCGTGGGCTTCGACCAGCTTTTGGTGGTCGGGCGTT
CGGAAACCGAAGGCGTGGATTTCCGTTACCGTATTTTCAATGCCGACGGCAGCGAGGTCGGGCAATGCGGCAACGGAGCG
CGTTGTTTTGCCCGTTTTGTTGCAGACAAGGGTTTGACCGATAAGAAAGAAATTTGTGTTGAAACGGCAAATGGCGTTAT
TTTTCCGAAATTGTCCGATAACGGCATGGTTACGGTCAATATGGGCAAACCGAAGTTTATGCCGTCTGAAATACCGTTTG
TCCCCGAATCGGGCGAGGGGGATGATGCCTGTATTTACGGGGTGCATCTCGAATCCGGCATTCAGCCTGTCAGCTGCGTC
AATATGGGCAACCCCCATGCGGTGATTGTGGTCGATGACGTAGAATGCGCGCCGGTGCGCGAAACCGGTTCGCTTATCGA
ACCGCACAGGCAGTTTCCCGAACGCGTCAACGTCGGCTTTATGCAGATTGTCAGCCGAACCGCGATTCGTTTGCGCGTGT
TCGAGCGCGGCGTGGGCGAAACCCAAGCGTGCGGTACGGGCGCGTGTGCGGCTGTGGTGGCGGGTATCCGTCTGGGGTTG
TTGGATGAAGGGAAAACGGTAGAGGTGATTTTGCCGGGCGGGACTTTATATATCGAATGGGCCTGCGGCGGCGATGTGAT
GATGACCGGCCCTGCGGAAGCGGTGTTTGAAGGCGAGTTGGCGTATTCATGA

Upstream 100 bases:

>100_bases
CTGATTTTAATCCTCAGGCGGCGGGAGATGGAAGCATTTCCCTTCGGTGCGGGGGATTTCGGATTCGGAAGCAACAGACG
ATACGGGATTTCGGAACAAT

Downstream 100 bases:

>100_bases
TTTTGCTGCATTTGGATTTTTTGTCTGCCTTACTGTATGCGGCGGTTTTTCTGTTTCTGATATTCCGCGCAGGAATGTTG
CAATGGTTTTGGGCGAGTAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVGFDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGA
RCFARFVADKGLTDKKEICVETANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGVGETQACGTGACAAVVAGIRLGL
LDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVFEGELAYS

Sequences:

>Translated_283_residues
MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVGFDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGA
RCFARFVADKGLTDKKEICVETANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGVGETQACGTGACAAVVAGIRLGL
LDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVFEGELAYS
>Mature_283_residues
MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVGFDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGA
RCFARFVADKGLTDKKEICVETANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGVGETQACGTGACAAVVAGIRLGL
LDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVFEGELAYS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=56.0714285714286, Blast_Score=286, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30393; Mature: 30393

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVGFDQLLVVGRSETEGVD
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCE
FRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVETANGVIFPKLSDNGMVTVN
EEEEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHEECCCCEEEEEECCCCEEEEE
MGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCVNMGNPHAVIVVDDVECAPVR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCC
ETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGVGETQACGTGACAAVVAGIRLGL
CCCCCCCHHHHCHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHEEE
LDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVFEGELAYS
ECCCCEEEEEECCCEEEEEEECCCCEEEECCCCCEEECCCCCC
>Mature Secondary Structure
MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVGFDQLLVVGRSETEGVD
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCE
FRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVETANGVIFPKLSDNGMVTVN
EEEEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHEECCCCEEEEEECCCCEEEEE
MGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCVNMGNPHAVIVVDDVECAPVR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCC
ETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGVGETQACGTGACAAVVAGIRLGL
CCCCCCCHHHHCHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHEEE
LDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVFEGELAYS
ECCCCEEEEEECCCEEEEEEECCCCEEEECCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]