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The map label for this gene is gcvT [H]

Identifier: 161869507

GI number: 161869507

Start: 540901

End: 542001

Strand: Direct

Name: gcvT [H]

Synonym: NMCC_0520

Alternate gene names: 161869507

Gene position: 540901-542001 (Clockwise)

Preceding gene: 161869494

Following gene: 161869508

Centisome position: 25.12

GC content: 54.41

Gene sequence:

>1101_bases
ATGATTACTCTGAAAACCACCCCATTTCATCAAGCCCATCAAGATGCAGGCGCGAAGCCGGTAGATTTTGCCGGCTGGGA
GCTGCCCATCCATTATGGTTCGCAAATCGCCGAACACGAAGCCGTGCGCACCGACGCCGGTATGTTTGACGTATCCCATA
TGCTTGTTACCGACGTAGCAGGCGCAAATGCCAAAGCCTTTTTCCGCAAATTGATTGCCAACGATGTCGCCAAGCTCGCT
TTTGTCGGCAAAGCCCTTTATTCCGCTTTGCTCAACGACAACGGCGGCGTGATTGACGACTTAATCGTTTACCGCACCAA
CGAAGCCGAAACCCAATACCGCATCGTGTCCAACGGCGCGACTCGCGAAAAAGATACGGCACAATTCCACAAAGTCGGAC
AAGAGTTCGGTGTTTCCTTCAATCCGCGCTACGACCTCGGCATGCTCGCCGTACAAGGTCCTAAAGCCATTGAAAAACTC
CTGACCGTCAAACCCGAATGGGCAGATGTCGTCCATAACCTCAAACCGTTCCAAGGCGCGGATTTGGGCAACGACTGGTT
TGTCGCCCGCACTGGCTATACCGGCGAAGACGGCGTGGAAGTCATCCTGCCCGGTACCGAAGCCGTTGCATTCTTCAAAG
CCCTGCAAGCAGCAGGCGTACATCCTTGCGGCCTCGGCGCACGCGACACCCTGCGCATGGAAGCCGGTATGAACCTCTAC
GGCAACGATATGGACGACAATACCAGTCCGCTCGAAGCAGGCATGGGTTGGACGGTTGATTTGAAAGACGAAAGCCGCGA
TTTTGTCGGTAAAACCGCTTTGCTGGCATTGAAAGAAAAAGGCGTTGCCGTCAAACAGGTCGGTCTGTTGCTCGACAAAG
GCGGCATCCTGCGCGCGCATATGGAAGTGTTGACCGACAAAGGCAAAGGCGAAACCACCAGCGGCGTATTCTCTCCAAGC
CTGAAACAATCCATCGCCATCGCCCGCGTGCCGAAAGACTTTGACGGCGATACCGCCAAAGTTCTGATTCGCGGCAAAGA
AGTGGACGTACGCGTACTGAAGCTGCCGTTTGTGCGCAACGGACAGAAACAGTTTGATTGA

Upstream 100 bases:

>100_bases
TGCGGATACGGCCGAAAATCTCAGGTTCAAGGACAGATAGGGTCATCCGCGCACAGGTGCGCGGGTGGCATCTGAACAAA
AAATCCGGAGAAACTTGAGA

Downstream 100 bases:

>100_bases
TGCGGTTTCAGACGGCATTTTCATTTCATATGCCGTCTGAAAGCAGGTTTTAATTGTTGTCCGATACGGACGTTTGTAGA
AAGCATTGAACAAGGCATCT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLA
FVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAAGVHPCGLGARDTLRMEAGMNLY
GNDMDDNTSPLEAGMGWTVDLKDESRDFVGKTALLALKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPS
LKQSIAIARVPKDFDGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD

Sequences:

>Translated_366_residues
MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLA
FVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAAGVHPCGLGARDTLRMEAGMNLY
GNDMDDNTSPLEAGMGWTVDLKDESRDFVGKTALLALKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPS
LKQSIAIARVPKDFDGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD
>Mature_366_residues
MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLA
FVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAAGVHPCGLGARDTLRMEAGMNLY
GNDMDDNTSPLEAGMGWTVDLKDESRDFVGKTALLALKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPS
LKQSIAIARVPKDFDGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=376, Percent_Identity=36.1702127659575, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI257796258, Length=359, Percent_Identity=35.6545961002786, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI257796254, Length=369, Percent_Identity=32.2493224932249, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI257796256, Length=322, Percent_Identity=34.7826086956522, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI24797151, Length=364, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI197927446, Length=306, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI21361378, Length=306, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789272, Length=364, Percent_Identity=52.1978021978022, Blast_Score=349, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17560118, Length=378, Percent_Identity=35.1851851851852, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71994052, Length=317, Percent_Identity=25.2365930599369, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=317, Percent_Identity=25.2365930599369, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320222, Length=394, Percent_Identity=34.2639593908629, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI20129441, Length=373, Percent_Identity=34.5844504021448, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39732; Mature: 39732

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVA
CEEECCCCCHHHHHHCCCCCCCCCCCEEEEHHCCHHHHHHHHHCCCCHHHHHHHHHHHCC
GANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEECCC
TREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKLLTVKPEWADVVHNLKPFQGA
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHCCCCCCCC
DLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAAGVHPCGLGARDTLRMEAGMNLY
CCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCC
GNDMDDNTSPLEAGMGWTVDLKDESRDFVGKTALLALKEKGVAVKQVGLLLDKGGILRAH
CCCCCCCCCCHHCCCCCEEEECCCCCHHHHHHHHEEHHHCCCCHHHHHEEECCCCCHHHH
MEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDFDGDTAKVLIRGKEVDVRVLKLPFVRN
HHHHHCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCC
GQKQFD
CHHCCC
>Mature Secondary Structure
MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVA
CEEECCCCCHHHHHHCCCCCCCCCCCEEEEHHCCHHHHHHHHHCCCCHHHHHHHHHHHCC
GANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEECCC
TREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKLLTVKPEWADVVHNLKPFQGA
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHCCCCCCCC
DLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAAGVHPCGLGARDTLRMEAGMNLY
CCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCC
GNDMDDNTSPLEAGMGWTVDLKDESRDFVGKTALLALKEKGVAVKQVGLLLDKGGILRAH
CCCCCCCCCCHHCCCCCEEEECCCCCHHHHHHHHEEHHHCCCCHHHHHEEECCCCCHHHH
MEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDFDGDTAKVLIRGKEVDVRVLKLPFVRN
HHHHHCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCC
GQKQFD
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]