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The map label for this gene is grpE [H]

Identifier: 161869494

GI number: 161869494

Start: 528768

End: 529346

Strand: Direct

Name: grpE [H]

Synonym: NMCC_0507

Alternate gene names: 161869494

Gene position: 528768-529346 (Clockwise)

Preceding gene: 161869492

Following gene: 161869507

Centisome position: 24.55

GC content: 54.75

Gene sequence:

>579_bases
ATGAGCGAACAGACACAGCAGCAAAACAGTGAAGAAGCGGTTGAAAATGTGGAGGCGGTGGAAACCGTCGAGACAGTAGG
AAATGCGGACGGTGTGCAGGAACAGGCTGCCGCAGAGCCGGCTTATGAGGATTTGCAGGCGCGGATTGCCGAGCTGGAAG
CGCAGTTGAAAGACGAGCAGCTGCGCGCTTTGGCAAACGAGCAAAACCTGCGCCGCCGCCACCAGCAGGAAATTGCGGAT
ACGCACAAGTTCGCCGGACAGAAGTTTGCCGTGGAAATGCTGCCGGTCAAGGATTATCTGGAAATGGCGCTTTTGGATCA
GAGCGGCAATTTCGATGCGCTGAAAATGGGCGTGCAGATGACTTTGAACGAGTTGCAGAAAGCATTTGATGCTACGCAAA
TCAAGGAAATCAATCCTAAAGCGGGCGATAAGCTCGATCCGAATATCCATCAGGCGATGCAGGCGGTGGCAAGCGAACAG
GAACCCAATACCGTGGTGGGCGTGATGAAGAAGGGTTATACGCTGTCCGACCGCGTGTTGCGCCCGGCTATGGTTACGGT
GGCACAGAAGGAAGCCTGA

Upstream 100 bases:

>100_bases
TTGAAATTCTCTGCTTTTGCTTGAAGTTTCTTGAAAATGTCCTTATCTTGCGCGGGTAATAACTGGATTTTGATTTCCAA
TTTGTTTTAAGGGATACGAT

Downstream 100 bases:

>100_bases
AGGCGTCTGGGGAATAATCTGATTTATTTCCTGAAGCGCGTTTTGCGTATAAACCGATCGAAGTAAAGCGGCAATGCCGT
CTGAACCCGCCTGTCGGGCT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYEDLQARIAELEAQLKDEQLRALANEQNLRRRHQQEIAD
THKFAGQKFAVEMLPVKDYLEMALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA

Sequences:

>Translated_192_residues
MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYEDLQARIAELEAQLKDEQLRALANEQNLRRRHQQEIAD
THKFAGQKFAVEMLPVKDYLEMALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA
>Mature_191_residues
SEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYEDLQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADT
HKFAGQKFAVEMLPVKDYLEMALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQE
PNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=173, Percent_Identity=36.4161849710983, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI1788967, Length=185, Percent_Identity=38.9189189189189, Blast_Score=103, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=160, Percent_Identity=33.75, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=199, Percent_Identity=31.6582914572864, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653432, Length=188, Percent_Identity=35.6382978723404, Blast_Score=83, Evalue=9e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21322; Mature: 21191

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYEDLQARIAELEAQLKDEQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEMALLDQSGNFDALKMGVQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
TLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQEPNTVVGVMKKGYTLSDRVL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
RPAMVTVAQKEA
HHHHHHHHHCCC
>Mature Secondary Structure 
SEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYEDLQARIAELEAQLKDEQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEMALLDQSGNFDALKMGVQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
TLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQEPNTVVGVMKKGYTLSDRVL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
RPAMVTVAQKEA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA