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The map label for this gene is rfbB2 [H]

Identifier: 161869097

GI number: 161869097

Start: 85412

End: 86479

Strand: Direct

Name: rfbB2 [H]

Synonym: NMCC_0087

Alternate gene names: 161869097

Gene position: 85412-86479 (Clockwise)

Preceding gene: 161869096

Following gene: 161869098

Centisome position: 3.97

GC content: 54.03

Gene sequence:

>1068_bases
ATGAGAAAAATCCTTGTTACCGGCGGTGCGGGCTTTATCGGCTCTGCCGTTGTCCGTCATATTATCCGAAACACCCAAGA
TTCCGTCGTCAACCTCGACAAACTGACCTACGCGGGCAATCTCGAATCGCTGACCGACATTGCCGATAATCCCCGCTATG
CTTTCGAGCAAGTGGACATTTGCGACCGCGCCGAACTCGACCGCGTATTCGCACAACACCGGCCCGATGCCGTGATGCAC
TTGGCGGCGGAAAGCCATGTCGACCGCTCTATCGGTTCGGCAGGCGAGTTTATCCAAACCAATATCGTCGGCACATTCAA
TCTGCTTGAAGCAGCCCGCGCCTACCGGCAACAAATGCCGTCTGAACAGCACGAAGCCTTCCGTTTCCACCATATTTCCA
CCGATGAAGTCTATGGCGATTTAGGCGGCACGGACGATTTGTTTACCGAAACCGCGCCCTACGCGCCGTCCAGCCCCTAC
TCTGCCTCTAAAGCGTCCAGCGACCACCTCGTCCGCGCGTGGTTGCGTACTTACGGCTTGCCGACCATTGTAACCAACTG
CTCCAACAACTACGGTCCTTACCATTTTCCGGAAAAACTCATTCCTTTGATGATTCTGAACGCGCTTGACGGCAAACCGC
TGCCTGTGTACGGCGACGGTATGCAAATCCGCGACTGGCTGTTTGTCGAAGACCACGCGCGCGCGCTGTATCAGGTTGTT
ACCGAAGGTGTTGTCGGCGAAACCTACAATATCGGCGGCCACAATGAAAAAGCCAATATTGAAGTCGTCAAAACCATCTG
CGCCCTGCTGGAAGAACTCGCTCCCGAAAAACCGGCCGGTGTGGCGCGTTATGAAGATTTGATTACTTTCGTACAAGACC
GCCCCGGCCATGACGTGCGCTACGCCATTGATGCAGCCAAAATCAGTAGGGAACTAGGCTGGAAACCGCAGGAAACCTTC
GAGTCCGGCCTCCGCAAAACCGTGCAATGGTATCTGGACAACAAAACCTGGTGGCAAAATGTATTGAACGGCAGCTATCG
TTTGGAACGTTTAGGTACTGGAAAATAG

Upstream 100 bases:

>100_bases
GCCGTCTGAAAGAGATGTTTTCAGACGGCCTCTTTATCTGAAAAACACACATTCTGTCTGCTATAATCTGTTTATATTTT
TTGGCTATCCTCTGAAATTT

Downstream 100 bases:

>100_bases
TTTTCAGACGGCATCCCGACGCAATGCCGTCTGAAAACCCATCGCAAAGGAAGAAAGAAAAGATGAAAGGCATCATACTG
GCAGGCGGCAGCGGCACGCG

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMH
LAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYRQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVV
TEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDVRYAIDAAKISRELGWKPQETF
ESGLRKTVQWYLDNKTWWQNVLNGSYRLERLGTGK

Sequences:

>Translated_355_residues
MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMH
LAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYRQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVV
TEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDVRYAIDAAKISRELGWKPQETF
ESGLRKTVQWYLDNKTWWQNVLNGSYRLERLGTGK
>Mature_355_residues
MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMH
LAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYRQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVV
TEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDVRYAIDAAKISRELGWKPQETF
ESGLRKTVQWYLDNKTWWQNVLNGSYRLERLGTGK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=36.0703812316716, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=24.4776119402985, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=76.9886363636364, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=72.5212464589235, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=25.4237288135593, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788366, Length=359, Percent_Identity=24.5125348189415, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788365, Length=349, Percent_Identity=22.0630372492837, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=37.0588235294118, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=33.8368580060423, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=25.2054794520548, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=25.0681198910082, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=23.5294117647059, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=372, Percent_Identity=28.7634408602151, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.9021739130435, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=23.4421364985163, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24158427, Length=277, Percent_Identity=24.5487364620939, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39800; Mature: 39800

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDI
CCEEEEECCCCHHHHHHHHHHHHCCHHHHEEHHHHEECCCHHHHHHHHCCCCHHHHHCCH
CDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYRQQMP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGL
CHHHHHHHHCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVV
CCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEHEEEEHHHHHHHHHHH
TEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDVR
HHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCE
YAIDAAKISRELGWKPQETFESGLRKTVQWYLDNKTWWQNVLNGSYRLERLGTGK
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCCCC
>Mature Secondary Structure
MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDI
CCEEEEECCCCHHHHHHHHHHHHCCHHHHEEHHHHEECCCHHHHHHHHCCCCHHHHHCCH
CDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYRQQMP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGL
CHHHHHHHHCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVV
CCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEHEEEEHHHHHHHHHHH
TEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGHDVR
HHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCE
YAIDAAKISRELGWKPQETFESGLRKTVQWYLDNKTWWQNVLNGSYRLERLGTGK
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]