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The map label for this gene is rfbA2 [H]

Identifier: 161869098

GI number: 161869098

Start: 86398

End: 87408

Strand: Direct

Name: rfbA2 [H]

Synonym: NMCC_0088

Alternate gene names: 161869098

Gene position: 86398-87408 (Clockwise)

Preceding gene: 161869097

Following gene: 161869099

Centisome position: 4.01

GC content: 51.73

Gene sequence:

>1011_bases
ATGGTATCTGGACAACAAAACCTGGTGGCAAAATGTATTGAACGGCAGCTATCGTTTGGAACGTTTAGGTACTGGAAAAT
AGTTTTCAGACGGCATCCCGACGCAATGCCGTCTGAAAACCCATCGCAAAGGAAGAAAGAAAAGATGAAAGGCATCATAC
TGGCAGGCGGCAGCGGCACGCGCCTCTACCCCATCACGCGCGGCGTATCCAAACAGCTCCTGCCCGTGTACGACAAACCG
ATGATTTATTACCCCTTGTCGGTTTTGATGCTGGCGGGAATCCGCGATATTTTGGTGATTACCGCGCCTGAAGACAACGC
CTCTTTCAAACGCCTGCTTGGCGACGGCAGCGATTTCGGCATTTCCATCAGTTATGCCGTGCAACCCAGTCCGGACGGCT
TGGCACAGGCATTTATCATCGGCGAAGAATTTATCGGCAACGACAATGTTTGCTTGGTTTTGGGCGACAATATTTTTTAC
GGTCAGTCGTTTACGCAAACATTGAAACAGGCGGCAGCGCAAACGCACGGCGCAACCGTGTTTGCTTATCAGGTCAAAAA
CCCCGAACGTTTCGGCGTGGTTGAATTTAACGAAAACTTCCGCGCCGTTTCCATCGAAGAAAAACCGCAACGGCCCAAAT
CCGATTGGGCGGTAACCGGCTTGTACTTCTACGACAACCGTGCCGTCGAGTTCGCCAAACAAATCAAACCGTCCGCACGC
GGCGAATTGGAAATTACCGACCTCAACCGGATGTATTTGGAAGACGGCTCGCTCTCCGTTCAAATATTGGGACGCGGTTT
CGCGTGGCTGGACACCGGTACGCACGAAAGCCTGCACGAGGCGGCTTCGTTCGTCCAAACCGTGCAAAACATCCAAAACC
TGCACATCGCCTGCCTGGAAGAAATCGCTTGGCGCAACGGTTGGCTTTCCGATGAAAAACTGGAAGAATTGGCGCGCCCG
ATGGCGAAAAACCAATACGGCCAATATTTGCTGCGCCTGTTGAAAAAATAA

Upstream 100 bases:

>100_bases
GCCATGACGTGCGCTACGCCATTGATGCAGCCAAAATCAGTAGGGAACTAGGCTGGAAACCGCAGGAAACCTTCGAGTCC
GGCCTCCGCAAAACCGTGCA

Downstream 100 bases:

>100_bases
TGTTTGAGGCCGTCTGAAACTTTTCAGACGGCCTTTAGCTGAAAGATAAAAAGATGAACATCATTGATACCGCCATTCCT
GACGTAAAACTGCTTGAGCC

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MVSGQQNLVAKCIERQLSFGTFRYWKIVFRRHPDAMPSENPSQRKKEKMKGIILAGGSGTRLYPITRGVSKQLLPVYDKP
MIYYPLSVLMLAGIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNVCLVLGDNIFY
GQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQIKPSAR
GELEITDLNRMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARP
MAKNQYGQYLLRLLKK

Sequences:

>Translated_336_residues
MVSGQQNLVAKCIERQLSFGTFRYWKIVFRRHPDAMPSENPSQRKKEKMKGIILAGGSGTRLYPITRGVSKQLLPVYDKP
MIYYPLSVLMLAGIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNVCLVLGDNIFY
GQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQIKPSAR
GELEITDLNRMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARP
MAKNQYGQYLLRLLKK
>Mature_336_residues
MVSGQQNLVAKCIERQLSFGTFRYWKIVFRRHPDAMPSENPSQRKKEKMKGIILAGGSGTRLYPITRGVSKQLLPVYDKP
MIYYPLSVLMLAGIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNVCLVLGDNIFY
GQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQIKPSAR
GELEITDLNRMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARP
MAKNQYGQYLLRLLKK

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=287, Percent_Identity=72.8222996515679, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788351, Length=286, Percent_Identity=65.034965034965, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1787488, Length=241, Percent_Identity=24.4813278008299, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788355, Length=237, Percent_Identity=25.3164556962025, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=183, Percent_Identity=22.9508196721311, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320148, Length=194, Percent_Identity=26.8041237113402, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355443, Length=228, Percent_Identity=25.8771929824561, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644084, Length=228, Percent_Identity=25.8771929824561, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 37868; Mature: 37868

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSGQQNLVAKCIERQLSFGTFRYWKIVFRRHPDAMPSENPSQRKKEKMKGIILAGGSGT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEEEECCCCC
RLYPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILVITAPEDNASFKRLLGDGSDFG
EEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCC
ISISYAVQPSPDGLAQAFIIGEEFIGNDNVCLVLGDNIFYGQSFTQTLKQAAAQTHGATV
EEEEEEECCCCCHHHHEEEEHHHHCCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCEE
FAYQVKNPERFGVVEFNENFRAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQIKPSAR
EEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECCHHHHHHHHCCCCCC
GELEITDLNRMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLE
CCEEEEECCEEEEECCCEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAWRNGWLSDEKLEELARPMAKNQYGQYLLRLLKK
HHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MVSGQQNLVAKCIERQLSFGTFRYWKIVFRRHPDAMPSENPSQRKKEKMKGIILAGGSGT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEEEECCCCC
RLYPITRGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILVITAPEDNASFKRLLGDGSDFG
EEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCC
ISISYAVQPSPDGLAQAFIIGEEFIGNDNVCLVLGDNIFYGQSFTQTLKQAAAQTHGATV
EEEEEEECCCCCHHHHEEEEHHHHCCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCEE
FAYQVKNPERFGVVEFNENFRAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQIKPSAR
EEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECCHHHHHHHHCCCCCC
GELEITDLNRMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLE
CCEEEEECCEEEEECCCEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAWRNGWLSDEKLEELARPMAKNQYGQYLLRLLKK
HHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]