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The map label for this gene is alkA [H]

Identifier: 161526313

GI number: 161526313

Start: 3437179

End: 3438150

Strand: Reverse

Name: alkA [H]

Synonym: Bmul_3144

Alternate gene names: 161526313

Gene position: 3438150-3437179 (Counterclockwise)

Preceding gene: 161526314

Following gene: 161526312

Centisome position: 99.7

GC content: 73.35

Gene sequence:

>972_bases
TTGAGCATCGAAATCGCAACGATCGCATCGACAGCGGACGGCGGCCACGTGCCGCCGTCCGCGCAACTGGAACTGCGCTT
CAAGGCGCCGTACGACTGGCCGCGCGTGCTGCGCTTTTTCAGCGGCCGCGCGATCCCCGGCGTGGAGCAGGTGGCGCACG
GCGTCTATCGACGCATCGTCGATCTGCACGGCGAACCGGGCCGCCTCACCGTGACGCGGCATCCGCGTCGGCATTGTCTG
ATCGCGACCGTCGAAGGCGCGGCGGCGCGCCACGTCGACGATGCATTCGCCGCACGTGTCGCGTCGATGTTCGATCTCGG
CGCGGACCCGGCCGCGATCGGCAGCGGGCTCGCGCGCGACCCGTGGTTCGCGCCGCTCGTGGCGGCCGCGCCGGGGCTGC
GCGTGCCGGGCGCGTGGTCGGGTTTCGAGCTGGCGGTGCGGGCGATCGTCGGCCAGCAGGTCAGCGTGAAGGCCGCGACG
ACGATCGTCGGGCGGCTCGTCGAACGGGCGGGCGAACGCGTGCTGCATGACGATGCAGATGCGGCCGACGGCGCGCCCGC
CTGGCGCTTTCCGACGCCGGACGCGCTCGCCGCGTGCGATCTCGCGAAGATCGGCATGCCGGGCAAGCGCGCGGCCGCGC
TGACCGGGATGGCGCGCGCGGTCGCGGCCGGCGACGTGCCGGTCGACACCGCGCAGGCCGATCTCGCGACGCTGCGCGCC
ACGTGGCTCGAACTGCCGGGCATCGGCCCGTGGACGGTCGAATACATCGCGATGCGCGCATGGCGCGATCCGGACGCTTG
GCCCGCGTCCGATCTGGTCCTGATGCAGTCGATCGCCGCGCGCGATCCGTCGCTCGACCGGCTCGCGAGCCAGAAGCAGC
GCACCGAGGGCTGGCGTCCGTGGCGCGCGTACGCGGCGCTGCATCTGTGGAACGAGGTCGCGGACCGGATGGGCGCCGCG
CGCGGCGGCTAG

Upstream 100 bases:

>100_bases
GGTGGGGCGTGCGCCGCAAGGCGACGCTGCTCGCATCCGAAGCGCGCCGCGCACGCGACGACGCACCGGCCACGGCCGCC
ATCGAGGAGGACAGCACAGC

Downstream 100 bases:

>100_bases
ACGCTATTGCATCGAAACCGGCGCTGCGTACGATGGCCGCGCGCCGCGTTCCGTCGGACGAATCCGCAAGCGCCTGCGCA
GCCGCCTCGCTGCATGCATC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase II, inducible; TAG II; DNA-3-methyladenine glycosidase II; DNA-3-methyladenine glycosylase II [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSIEIATIASTADGGHVPPSAQLELRFKAPYDWPRVLRFFSGRAIPGVEQVAHGVYRRIVDLHGEPGRLTVTRHPRRHCL
IATVEGAAARHVDDAFAARVASMFDLGADPAAIGSGLARDPWFAPLVAAAPGLRVPGAWSGFELAVRAIVGQQVSVKAAT
TIVGRLVERAGERVLHDDADAADGAPAWRFPTPDALAACDLAKIGMPGKRAAALTGMARAVAAGDVPVDTAQADLATLRA
TWLELPGIGPWTVEYIAMRAWRDPDAWPASDLVLMQSIAARDPSLDRLASQKQRTEGWRPWRAYAALHLWNEVADRMGAA
RGG

Sequences:

>Translated_323_residues
MSIEIATIASTADGGHVPPSAQLELRFKAPYDWPRVLRFFSGRAIPGVEQVAHGVYRRIVDLHGEPGRLTVTRHPRRHCL
IATVEGAAARHVDDAFAARVASMFDLGADPAAIGSGLARDPWFAPLVAAAPGLRVPGAWSGFELAVRAIVGQQVSVKAAT
TIVGRLVERAGERVLHDDADAADGAPAWRFPTPDALAACDLAKIGMPGKRAAALTGMARAVAAGDVPVDTAQADLATLRA
TWLELPGIGPWTVEYIAMRAWRDPDAWPASDLVLMQSIAARDPSLDRLASQKQRTEGWRPWRAYAALHLWNEVADRMGAA
RGG
>Mature_322_residues
SIEIATIASTADGGHVPPSAQLELRFKAPYDWPRVLRFFSGRAIPGVEQVAHGVYRRIVDLHGEPGRLTVTRHPRRHCLI
ATVEGAAARHVDDAFAARVASMFDLGADPAAIGSGLARDPWFAPLVAAAPGLRVPGAWSGFELAVRAIVGQQVSVKAATT
IVGRLVERAGERVLHDDADAADGAPAWRFPTPDALAACDLAKIGMPGKRAAALTGMARAVAAGDVPVDTAQADLATLRAT
WLELPGIGPWTVEYIAMRAWRDPDAWPASDLVLMQSIAARDPSLDRLASQKQRTEGWRPWRAYAALHLWNEVADRMGAAR
GG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=294, Percent_Identity=36.3945578231293, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 34527; Mature: 34395

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIATIASTADGGHVPPSAQLELRFKAPYDWPRVLRFFSGRAIPGVEQVAHGVYRRIV
CCEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DLHGEPGRLTVTRHPRRHCLIATVEGAAARHVDDAFAARVASMFDLGADPAAIGSGLARD
HCCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
PWFAPLVAAAPGLRVPGAWSGFELAVRAIVGQQVSVKAATTIVGRLVERAGERVLHDDAD
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
AADGAPAWRFPTPDALAACDLAKIGMPGKRAAALTGMARAVAAGDVPVDTAQADLATLRA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TWLELPGIGPWTVEYIAMRAWRDPDAWPASDLVLMQSIAARDPSLDRLASQKQRTEGWRP
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
WRAYAALHLWNEVADRMGAARGG
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIEIATIASTADGGHVPPSAQLELRFKAPYDWPRVLRFFSGRAIPGVEQVAHGVYRRIV
CEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DLHGEPGRLTVTRHPRRHCLIATVEGAAARHVDDAFAARVASMFDLGADPAAIGSGLARD
HCCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
PWFAPLVAAAPGLRVPGAWSGFELAVRAIVGQQVSVKAATTIVGRLVERAGERVLHDDAD
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
AADGAPAWRFPTPDALAACDLAKIGMPGKRAAALTGMARAVAAGDVPVDTAQADLATLRA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TWLELPGIGPWTVEYIAMRAWRDPDAWPASDLVLMQSIAARDPSLDRLASQKQRTEGWRP
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
WRAYAALHLWNEVADRMGAARGG
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6094528; 9097040; 9278503; 6389535; 3529081; 8706135; 8706136 [H]